Article
Microbiology
Neele J. Froboese, Ioana D. Olaru, Julia Sophie Schneider, Wenlan Zhang, Alexander Mellmann, Franziska Schuler, Tobias Grebe, Frieder Schaumburg
Summary: The study aimed to assess the impact of selective pre-PCR enrichment on the performance of RIDA (R) GENE EHEC/EPEC for detecting diarrheagenic Escherichia coli from stool samples. Both stx1/2 and eae were analyzed in the 250 samples with and without pre-PCR enrichment. The results showed that pre-PCR enrichment did not improve the test performance, as the sensitivities and specificities of both methods were similar.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Yaqi Song, Min Chen, Lu Han, Zhongyu Yan, Leiqing Pan, Kang Tu
Summary: In this study, a novel sensing platform based on fluorescence quenching was developed for rapid and sensitive detection of E. coli. The platform combined alendronic acid coated upconversion nanoparticles and Nile Blue with polymerase chain reaction (PCR). The sensing platform showed a detection limit of 33 CFU mL-1 and could detect E. coli within 1.5 hours. The study also discussed the sensitivity of the fluorescence sensor under different PCR cycle numbers and demonstrated the potential of constructing PCR fluorescence sensors for detecting E. coli.
ANALYTICA CHIMICA ACTA
(2023)
Article
Multidisciplinary Sciences
Babak Pakbin, Afshin Akhondzadeh Basti, Ali Khanjari, Wolfram Manuel Bruck, Leila Azimi, Abdollah Karimi
Summary: A PCR-HRM assay targeting the rrsA gene was designed and developed in this study to differentiate Shigella species. The assay demonstrated good sensitivity and specificity in distinguishing the Shigella isolates from clinical and food samples, indicating its potential application in public health and food safety surveillance systems.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Jude Fonbah Leinyuy, Innocent Mbulli Ali, Karimo Ousenu, Christopher B. Tume
Summary: This study analyzed 200 Enterobacteriaceae isolates resistant and susceptible to ESBL, quinolones, and aminoglycosides. It found a high carriage of resistance genes, but the carriage of resistance genes may not always be correlated with phenotypic expression.
Article
Engineering, Environmental
Naveen Joseph, Jane Lucas, Nikhil Viswanath, Reed Findlay, Jim Sprinkle, Michael S. Strickland, Eric Winford, Alan S. Kolok
Summary: The United States National Forests are multi-use lands that support human recreation and cattle grazing. Fecal contamination in the Mink Creek watershed is found to be caused by human activities and unknown sources.
Article
Multidisciplinary Sciences
Mojisola C. Hosu, Sandeep D. Vasaikar, Grace E. Okuthe, Teke Apalata
Summary: The study evaluated antimicrobial resistance patterns of P. aeruginosa, identifying a high rate of multi-drug resistant strains carrying bla(TEM) and bla(SHV) genes in the Eastern Cape. Continuous monitoring and strict compliance with infection prevention and control practices are crucial in preventing the spread of MDR P. aeruginosa.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Byron M. Berenger, Linda Chui, Christina Ferrato, Tracie Lloyd, Vincent Li, Dylan R. Pillai
Summary: This study compared the ability of four commercially available RT-PCR tests to detect five major bacterial enteric pathogens. The results showed high concordance rates between RT-PCR and culture for most pathogens, except for Yersinia. Only one of the assays could detect a specific subtype, while none could detect two specific bacterial species. Depending on the prevalence of these pathogens, culture methods may still be critical for their detection.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Food Science & Technology
Shan Shan, Yanmei Huang, Zhaohong Huang, Zhonger Long, Chengwei Liu, Xuelong Zhao, Keyu Xing, Xiaoyue Xiao, Jintao Liu, Yunhong Huang, Weihua Lai, Daofeng Liu
Summary: In this study, a method for accurate and rapid subtyping of STEC single colonies was developed using asPCR combined with LFIA, allowing for identification and subtyping of STEC with different Shiga toxin gene types.
Article
Microbiology
Mitul A. Patel, Aparna Pandey, A. C. Patel, S. S. Patel, H. C. Chauhan, M. D. Shrimali, Pankaj A. Patel, S. K. Mohapatra, B. S. Chandel
Summary: This study revealed the molecular characteristics and high occurrence of ESBL E. coli in poultry in Gujarat, India, mainly attributed to the genes bla(TEM), bla(SHV), and bla(OXA).
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Xiaoxiao Gu, Xue Ma, Qin Wu, Qiaoxiaoci Tao, Yingjin Chai, Xia Zhou, Mengli Han, Jie Li, Xin Huang, Tongzhong Wu, Xingxing Zhang, Fagang Zhong, Yiheng Cao, Liyuan Zhang
Summary: This study investigated the phylogenetic groups, virulence genes, and antibiotic resistance characteristics of pathogenic Escherichia coli isolates in Xinjiang, China. The majority of isolates belonged to phylogenetic groups A and B1, with the highest detection rates of the virulence genes crl and hlyE. The isolates showed the highest rates of resistance against streptomycin. These characteristics present challenges for the prevention and treatment of E. coli-related diseases in Xinjiang.
VETERINARY MEDICINE AND SCIENCE
(2023)
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Multidisciplinary Sciences
Joseph Blondeau, Marthe Kenny Charles, Vivian Loo, Heather Adam, Marcela Gonzalez Del Vecchio, Christiane Ghakis, Emma O'Callaghan, Radwan El Ali
Summary: This study analyzed 5 years of Canadian data to identify the predominant pathogens and antimicrobial resistance patterns in adult Gram-negative infections. The results showed that E. coli, P. aeruginosa, and K. pneumoniae were the most frequent isolates, and P. aeruginosa was the most common respiratory pathogen. The analysis of these national data can contribute to antimicrobial stewardship and guide treatment policies for adult patient care.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Review
Parasitology
Ineka Gow, Nicholas C. Smith, Damien Stark, John Ellis
Summary: Leishmania infections pose a significant health issue, primarily affecting impoverished regions. Research on accurate diagnosis and prevention using molecular methods like polymerase chain reaction is of great importance.
PARASITES & VECTORS
(2022)
Article
Infectious Diseases
Samanta Freire, Teresa Grilo, Laurent Poirel, Marta Aires-de-Sousa
Summary: This study investigated the potential role of urban pigeons in the dissemination of multidrug-resistant bacteria. The results showed that these pigeons can carry ESBL-producing Escherichia coli strains, which may contribute to the spread of antimicrobial resistance in the environment.
Review
Pediatrics
Valerie Sung, Frank D'Amico, Michael D. Cabana, Kim Chau, Gideon Koren, Francesco Savino, Hania Szajewska, Girish Deshpande, Christophe Dupont, Flavia Indrio, Silja Mentula, Anna Partty, Daniel Tancredi
Article
Obstetrics & Gynecology
Francesco Savino, Ilaria Galliano, Maria Garro, Andrea Savino, Paolo Manzoni, Valentina Dapra, Paola Montanari, Massimiliano Bergallo
AMERICAN JOURNAL OF PERINATOLOGY
(2018)
Article
Environmental Sciences
Andrea Lucchi, Edith Ladurner, Andrea Iodice, Francesco Savino, Renato Ricciardi, Francesca Cosci, Giuseppe Conte, Giovanni Benelli
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2018)
Article
Pediatrics
Francesco Savino, Maria Garro, Paola Montanari, Ilaria Galliano, Massimiliano Bergallo
JOURNAL OF PEDIATRICS
(2018)
Review
Nutrition & Dietetics
Silvia Salvatore, Francesco Savino, Maartje Singendonk, Merit Tabbers, Marc A. Benninga, Annamaria Staiano, Yvan Vandenplas
Editorial Material
Nutrition & Dietetics
Yvan Vandenplas, Francesco Savino
Article
Pediatrics
Francesco Savino, Ilaria Galliano, Andrea Savino, Valentina Dapra, Paola Montanari, Cristina Calvi, Massimiliano Bergallo
FRONTIERS IN PEDIATRICS
(2019)
Article
Immunology
Massimiliano Bergallo, Ilaria Galliano, Paola Montanari, Elena Zaniol, Elisa Graziano, Cristina Calvi, Carla Alliaudi, Valentina Dapra, Francesco Savino
MICROBES AND INFECTION
(2020)
Article
Nutrition & Dietetics
Francesco Savino, Paola Montanari, Ilaria Galliano, Valentina Dapra, Massimiliano Bergallo
Article
Nutrition & Dietetics
Simone Ceratto, Francesco Savino, Silvia Vannelli, Luisa De Sanctis, Francesca Giuliani
Article
Chemistry, Multidisciplinary
Federica Dal Bello, Enrica Mecarelli, Daniela Gastaldi, Francesco Savino, Claudio Medana
APPLIED SCIENCES-BASEL
(2020)
Article
Nutrition & Dietetics
Francesco Savino, Francesca Giuliani, Stefano Giraudi, Ilaria Galliano, Paola Montanari, Valentina Dapra, Massimiliano Bergallo
Summary: The aim of this study was to assess the serum values of IL-4, IL-5, IL-10, and IL-13 in infants with non-IgE mediated food allergies treated with a hydrolyzed formula. The study found that the levels of IL-4 and IL-13 were significantly higher in the non-IgE food allergy group compared to the control group, while IL-5 and IL-10 levels were significantly lower.
Article
Medicine, General & Internal
Francesco Savino, Valentina Dapra, Andrea Savino, Cristina Calvi, Paola Montanari, Ilaria Galliano, Massimiliano Bergallo
Summary: This study aimed to measure IFN-gamma and IDO1 values in infants' White blood cells during respiratory tract infections. The results showed a significant reduction in IFN-gamma expression in infants with RSV bronchiolitis, compared with healthy controls, while there was no significant difference in IDO1 expression between the two groups. New investigations focusing on RSV-infected subjects at different stages of infancy could provide further insights into the relationship between IDO1 expression and age.
Article
Pediatrics
Simone Ceratto, Francesco Savino
ITALIAN JOURNAL OF PEDIATRICS
(2019)
Review
Medicine, General & Internal
Morris Gordon, Elena Biagioli, Miriam Sorrenti, Carla Lingua, Lorenzo Moja, Shel S. C. Banks, Simone Ceratto, Francesco Savino
COCHRANE DATABASE OF SYSTEMATIC REVIEWS
(2018)