Article
Chemistry, Analytical
Xufang Hu, Zongping Wang, Haolin Chen, An Zhao, Nianrong Sun, Chunhui Deng
Summary: An advanced metabolic analysis tool based on highly porous metal oxides derived from metal-organic frameworks (MOFs) is developed in this study. The tool successfully differentiates and stages renal cell carcinoma (RCC), as well as identifies the size of tumors. This achievement shows promising prospects for designer matrix-based analytical tools in disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Article
Medicine, General & Internal
Henning Austermann, Frank Schaeffel, Ute Mathis, Verena Hund, Frank Musshoff, Focke Ziemssen, Sven Schnichels
Summary: Low-dose atropine eye drops have been shown to inhibit myopia in children and juveniles, but information on manufacturing and composition is lacking. A study on corneal penetration found that preservatives did not enhance the penetration of atropine, at least in an ex vivo setting.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Chenjie Yang, Aizhu Miao, Chaochao Yang, Chuwen Huang, Haolin Chen, Yongxiang Jiang, Chunhui Deng, Nianrong Sun
Summary: This study introduces a metabolic analysis tool for clinical coping with cataracts, which can successfully differentiate different types of cataracts and reveal the significant impact of diabetes and high myopia on cataracts.
Article
Chemistry, Analytical
Ying Feng, Xianhu Wei, Moutong Chen, Honghui Zhu, Jumei Zhang, Youxiong Zhang, Liang Xue, Lanyan Huang, Guoyang Chen, Qihui Gu, Shi Wu, Yu Ding, Qingping Wu
Summary: This study developed a new deep semi-quantitative fingerprinting method using pseudotargeted metabolomics and deep learning techniques, achieving a prediction accuracy of Listeria monocytogenes serotypes exceeding 99%. This technology demonstrates great potential for the rapid and accurate identification of pathogens.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Katerina V. Djambazova, Jacqueline M. Van Ardenne, Jeffrey M. Spraggins
Summary: Imaging mass spectrometry allows for the untargeted mapping of biomolecules directly from tissue sections, with widespread clinical applicability in disease diagnosis and classification. This review summarizes the two most widely employed IMS technologies and their advancements in biomedical applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Margaux Fresnais, Siwen Liang, Deniz Seven, Nevena Prodanovic, Julia Sundheimer, Walter E. Haefeli, Juergen Burhenne, Remi Longuespee
Summary: The development and validation of desorption/ionization mass spectrometric assays for drug quantification in tissue sections is crucial for their widespread application in (clinical) pharmacology. Advances in desorption electrospray ionization have demonstrated its usefulness for developing targeted quantification methods that meet validation requirements. Factors such as desorption spot morphology, analytical time, and sample surface need to be considered for successful method development. This study highlights the importance of considering desorption kinetics during DESI analyses, which can help reduce analytical time, verify solvent-based drug extraction methods, and predict feasibility of imaging assays. These findings provide valuable guidance for future development of validated DESI-profiling and imaging methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Xiaowei Song, Qingce Zang, Chao Li, Tianhao Zhou, Richard N. N. Zare
Summary: We developed an immunoassay-based desorption electrospray ionization mass spectrometry imaging (immuno-DESI-MSI) technique for visualizing functional macromolecules. Boronic acid mass tags (BMTs) were synthesized to label antibodies as MSI probes, which can be released from the antibody by cleaving the boronic ester bond under acidic conditions. The BMTs can work in both optical and MS imaging modes and can be readily identified due to the introduction of the boron element.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Sangwon Cha, Gyouwoong Jun, Yougyeong Park, Sung Jun An, Donghoon Lee
Summary: Nanospray desorption electrospray ionization (nano-DESI) was used to analyze the metabolite fingerprints on Hypericum leaves. The intensity changes of key metabolites over time were observed through full mass scan experiments and monitored using selected ion monitoring and pseudo-selected reaction monitoring experiments. The correlation between the time-dependent changes in the metabolite fingerprints and depth-wise metabolite distribution was examined by removing the surface layers of the leaves multiple times.
Article
Chemistry, Analytical
Xuetong Qu, Tao Wang, Xingyue Liu, Xinrong Jiang, Xiao Liang, Jianmin Wu
Summary: A dual-mechanism-driven strategy for high-coverage detection of serum lipids was proposed, using a novel SALDI-MS target composed of composite nanostructures. The strategy enabled simultaneous detection of multiple lipid types in a fast analysis. Furthermore, the method demonstrated high sensitivity and specificity in diagnosing hepatocellular carcinoma.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Eva Gato, Manuel J. Arroyo, Gema Mendez, Ana Candela, Bruno Kotska Rodino-Janeiro, Javier Fernandez, Belen Rodriguez-Sanchez, Luis Mancera, Jorge Arca-Suarez, Alejandro Beceiro, German Bou, Marina Oviano
Summary: This study proposes a machine learning approach that uses the complete cell spectra for the direct prediction of carbapenemase-producing Klebsiella pneumoniae (CPK) isolates. The method achieved high accuracy and revealed the contribution of the complete proteome to the algorithm-based classification.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Pharmacology & Pharmacy
Ines Garcia Del Valle, Carmen Alvarez-Lorenzo
Summary: Atropine, an old-known drug, has gained increasing attention for its therapeutic effects on eye structures. When administered topically, attention should be paid to atropine ocular pharmacokinetics and its ability to access the posterior segment. There is an urgent need to design formulations that can selectively deliver atropine to the target tissue for each specific application.
EXPERT OPINION ON DRUG DELIVERY
(2021)
Article
Chemistry, Analytical
Fengjian Chu, Gaosheng Zhao, Wangyu Li, Wei Wei, Weiwei Chen, Zihan Ma, Zhan Gao, Nazifi Sani Shuaibu, Jikui Luo, Bingwen Yu, Hongru Feng, Yuanjiang Pan, Xiaozhi Wang
Summary: A microwave plasma torch-based ion/molecular reactor (MPTIR) has been developed for rapid oxidation reactions. This device can activate oxygen in the air to achieve oxidation of thioethers, alcohols, and various environmental pollutants on a millisecond scale without the use of external oxidants or catalysts. It can also accurately predict environmental pollutants and study the mechanism of atmospheric oxidative processes.
ANALYTICAL CHEMISTRY
(2023)
Review
Neuroimaging
Keng Siang Lee, Isabel Siow, John J. Y. Zhang, Nicholas L. Syn, Conor S. Gillespie, Linus Z. H. Yuen, Gopinathan Anil, Cunli Yang, Bernard P. L. Chan, Vijay Kumar Sharma, Hock-Luen Teoh, Jing Mingxue, Kevin Soon Hwee Teo, May Zin Myint, Pervinder Bhogal, Lukas Meyer, Stefan Schob, Ching-Hui Sia, Anastasios Mpotsaris, Volker Maus, Tommy Andersson, Fabian Arnberg, Vamsi Krishna Gontu, Tsong-Hai Lee, Benjamin Y. Q. Tan, Leonard L. L. Yeo
Summary: In patients with acute ischemic stroke due to basilar artery occlusion, bridging intravenous thrombolysis was associated with lower mortality rates at 90 days compared with direct mechanical thrombectomy. However, there were no improved functional outcomes or increased symptomatic intracranial hemorrhage or subarachnoid hemorrhage between the two treatment arms. However, patients with large artery atherosclerosis benefited from bridging intravenous thrombolysis, with better functional outcomes.
JOURNAL OF NEUROINTERVENTIONAL SURGERY
(2023)
Review
Cardiac & Cardiovascular Systems
Tiffany T. S. Ye, Qi Zhuang Siah, Benjamin Y. Q. Tan, Jamie S. Y. Ho, Nicholas L. X. Syn, Yao Hao Teo, Yao Neng Teo, James W. Yip, Tiong-Cheng Yeo, Weiqin Lin, Raymond C. C. Wong, Ping Chai, Bernard Chan, Vijay Kumar Sharma, Leonard L. L. Yeo, Ching-Hui Sia
Summary: Hypertrophic cardiomyopathy patients are at risk of acute cerebrovascular events, such as ischemic stroke, transient ischemic attack, and systemic thromboembolism. Atrial fibrillation further increases this risk. The presence of atrial fibrillation in hypertrophic cardiomyopathy patients significantly raises the risk of thromboembolic events, including ischemic stroke and transient ischemic attack.
JOURNAL OF THROMBOSIS AND THROMBOLYSIS
(2023)
Article
Surgery
Darren W. Chua, Nicholas Syn, Ye-Xin Koh, Jin-Yao Teo, Peng-Chung Cheow, Alexander Y. F. Chung, Chung-Yip Chan, Brian K. P. Goh
Summary: This study analyzed the effects of body mass index (BMI) and standardized liver volume (SLV) on outcomes in minimally invasive liver resections (MILR). The results showed that high BMI and SLV were associated with increased blood loss and operative conversions, while low BMI was associated with a higher risk of major complications.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2023)
Article
Cardiac & Cardiovascular Systems
Yip Han Chin, Oliver Lim, Chaoxing Lin, Yu Yi Chan, Gwyneth Kong, Cheng Han Ng, Bryan Chong, Nicholas Syn, Kai En Chan, Mark D. Muthiah, Mohammad Shadab Siddiqui, Jiong-Wei Wang, Gemma Figtree, Mark Y. Chan, Nicholas W. S. Chew
Summary: This study analyzed data from placebo-controlled arms without background LLT and found that lipid-lowering therapy has a modest placebo effect but prominent nocebo effect, especially in secondary prevention RCTs, with musculoskeletal, headache, and gastrointestinal symptoms being the most affected.
EUROPEAN HEART JOURNAL-QUALITY OF CARE AND CLINICAL OUTCOMES
(2023)
Article
Endocrinology & Metabolism
Calesta Hui Yi Teo, Molly Tzu-Yu Lin, Isabelle Xin Yu Lee, Siew-Kwan Koh, Lei Zhou, Dylan Shaoying Goh, Hyungwon Choi, Hiromi Wai Ling Koh, Amanda Yun Rui Lam, Paik Shia Lim, Jodhbir S. Mehta, Jean-Paul Kovalik, Thomas M. Coffman, Hong Chang Tan, Yu-Chi Liu
Summary: In this study, fenofibrate, a PPAR-alpha agonist, was shown to significantly promote corneal nerve regeneration and reduce nerve edema in patients with DCN. It also improved corneal epithelial cell morphology and alleviated ocular surface neuroinflammation. The mechanisms of action may involve modulation of neurotrophin signaling pathway and lipid metabolism.
Review
Clinical Neurology
Keith Zhi Xian Toh, Ming Yi Koh, Enver De Wei Loh, Gabriel Yi Ren Kwok, Yao Hao Teo, Yao Neng Teo, Claire Xin Yi Goh, Nicholas Li Xun Syn, Andrew Fu Wah Ho, Ching-Hui Sia, Patrick A. Brouwer, Tommy Andersson, Lukas Meyer, Jens Fiehler, Pervinder Bhogal, Vijay K. Sharma, Benjamin Y. Q. Tan, Leonard L. L. Yeo
Summary: This systematic review and meta-analysis compared the efficacy and safety of stent retrievers (SR) and aspiration catheters (AC) in the treatment of acute ischaemic stroke due to distal medium vessel occlusion (AIS-DMVO). The results showed that SR/PC had higher odds of functional independence and lower odds of mortality compared to AC alone. However, further trials are needed to validate the efficacy and safety of SR use in AIS-DMVO.
EUROPEAN STROKE JOURNAL
(2023)
Article
Oncology
Roberto Montalti, Mariano Cesare Giglio, Andrew G. R. Wu, Federica Cipriani, Mizelle D'Silva, Amal Suhool, Phan Phuoc Nghia, Yutaro Kato, Chetana Lim, Paulo Herman, Fabricio Ferreira Coelho, Moritz Schmelzle, Johann Pratschke, Davit L. Aghayan, Qiu Liu, Marco V. Marino, Andrea Belli, Adrian K. H. Chiow, Iswanto Sucandy, Arpad Ivanecz, Fabrizio Di Benedetto, Sung Hoon Choi, Jae Hoon Lee, James O. Park, Mikel Prieto, Yoelimar Guzman, Constantino Fondevila, Mikhail Efanov, Fernando Rotellar, Gi-Hong Choi, Ricardo Robles-Campos, Xiaoying Wang, Robert P. Sutcliffe, Chung Ngai Tang, Charing C. Chong, Mathieu D'Hondt, Bernardo Dalla Valle, Andrea Ruzzenente, T. Peter Kingham, Olivier Scatton, Rong Liu, Alejandro Mejia, Kohei Mishima, Go Wakabayashi, Santiago Lopez-Ben, Franco Pascual, Daniel Cherqui, Fabio Forchino, Alessandro Ferrero, Giuseppe Maria Ettorre, Giovanni Battista Levi Sandri, Atsushi Sugioka, Bjorn Edwin, Tan-To Cheung, Tran Cong Duy Long, Mohammad Abu Hilal, Luca Aldrighetti, David Fuks, Ho-Seong Han, Roberto I. Troisi, Brian K. P. Goh, Nicholas L. Syn, Mikel Gastaca, Juul Meurs, Celine De Meyere, Eric C. H. Lai, Felix Krenzien, Prashant Kadam, Kit-Fai Lee, Diana Salimgereeva, Ruslan Alikhanov, Lip-Seng Lee, Jae Young Jang, Kevin P. Labadie, Masayuki Kojima, Asmund Avdem Fretland, Jacob Ghotbi, Jaime Arthur Pirola Kruger, Victor Lopez-Lopez, Paolo Magistri, Marco Colasanti, Margarida Robert, Mansour Saleh, Edoardo Poletto
Summary: This study aimed to evaluate the risk factors and outcomes of open conversion during minimally invasive liver surgery, including the impact of different approaches (laparoscopic vs. robotic) on conversions. Male sex, laparoscopic approach, cirrhosis, previous abdominal surgery, concomitant other surgery, higher ASA score, larger tumor size, conventional surgery, and specific procedure types were associated with an increased risk of conversion. While robotic assistance reduced the risk of conversion, converted robotic procedures had worse outcomes compared to converted laparoscopic procedures, including increased blood loss, blood transfusion rate, major complications, and mortality rates.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Article
Medicine, Research & Experimental
Yunzhe Li, Yunran Hu, Lingling Yang, Jingshu Liu, Chenxi Cui, Muyao Yang, Dongling Zou, Lei Zhou, Qi Zhou, Weihong Ge, Tingyuan Lang
Summary: In this study, it is found that luteolin, a natural flavonoid, directly binds to KDM4C and suppresses the stemness of ovarian cancer stem cells (OCSCs) by epigenetically inhibiting the PPP2CA/YAP axis. The maximal non-toxic dose of luteolin reduced the stemness properties of OCSCs, including sphere-forming capacity and the expression of OCSCs markers. Mechanistically, luteolin blocked KDM4C-induced histone demethylation of the PPP2CA promoter, leading to the inhibition of PPP2CA transcription and YAP dephosphorylation, thus attenuating YAP activity and the stemness of OCSCs.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Oncology
Zewei Chen, Mengqiu Yin, Junhao Fu, Shian Yu, Nicholas L. Syn, Darren W. Chua, T. Peter Kingham, Wanguang Zhang, Tijs J. Hoogteijling, Davit L. Aghayan, Tiing Foong Siow, Olivier Scatton, Paulo Herman, Marco V. Marino, Vincenzo Mazzaferro, Adrian K. H. Chiow, Iswanto Sucandy, Arpad Ivanecz, Sung Hoon Choi, Jae Hoon Lee, Mikel Prieto, Marco Vivarelli, Felice Giuliante, Andrea Ruzzenente, Chee-Chien Yong, Safi Dokmak, Constantino Fondevila, Mikhail Efanov, Zenichi Morise, Fabrizio Di Benedetto, Raffaele Brustia, Raffaele Dalla Valle, Ugo Boggi, David Geller, Andrea Belli, Riccardo Memeo, Salvatore Gruttadauria, Alejandro Mejia, James O. Park, Fernando Rotellar, Gi-Hong Choi, Ricardo Robles-Campos, Xiaoying Wang, Robert P. Sutcliffe, Johann Pratschke, Eric C. H. Lai, Charing C. N. Chong, Mathieu D'Hondt, Kazuteru Monden, Santiago Lopez-Ben, Qu Liu, Rong Liu, Alessandro Ferrero, Giuseppe Maria Ettorre, Federica Cipriani, Daniel Cherqui, Xiao Liang, Olivier Soubrane, Go Wakabayashi, Roberto I. Troisi, Tan-To Cheung, Yutaro Kato, Atsushi Sugioka, Ho-Seong Han, Tran Cong duy Long, David Fuks, Mohammad Abu Hilal, Luca Aldrighetti, Kuo-Hsin Chen, Bjorn Edwin, Brian K. P. Goh
Summary: This study evaluates the impact of body mass index (BMI) on the perioperative outcomes of laparoscopic left lateral sectionectomy (L-LLS). The results show that increasing BMI is associated with increased difficulty in L-LLS, including increased blood loss, open conversions, longer operative time, use of Pringles maneuver, and reduced length of stay.
Article
Gastroenterology & Hepatology
Andrea Ruzzenente, Bernardo Dalla Valle, Edoardo Poletto, Nicholas L. Syn, Tousif Kabir, Atsushi Sugioka, Federica Cipriani, Daniel Cherqui, Ho-Seong Han, Thomas Armstrong, Tran Cong Duy Long, Olivier Scatton, Paolo Herman, Johann L. Pratschke, Davit Aghayan, Rong Liu, Marco Marino, Adrian K. H. Chiow, Iswanto Sucandy, Arpad Ivanecz, Marco Vivarelli, Fabrizio Di Benedetto, Sung-Hoon Choi, Jae Hoon Lee, Mikel Prieto, Constantino Fondevila, Mikhail Efanov, Fernando Rotellar, Gi-Hong Choi, Ricardo Robles-Campos, Xiaoying P. Wang, Robert Sutcliffe, Eric C. H. Lai, Charing Chong, Mathieu D'Hondt, Chee Chien Yong, Roberto Troisi, T. Peter Kingham, Alessandro Ferrero, Giovanni Battista Levi Sandri, Olivier Soubrane, Mengqiu Yin, Santiago Lopez-Ben, Vincenzo Mazzaferro, Felice Giuliante, Kazateru Monden, Kohei Mishima, Go Wakabayashi, Tan-To Cheung, David Fuks, Mohammad Abu Hilal, Kuo-Hsin Chen, Luca Aldrighetti, Bjorn Edwin, Brian K. P. Goh
Summary: This multicentric study aimed to investigate the impact of tumor location and size on the difficulty of Laparoscopic- Left Hepatectomy (L- LH). The study found that tumors larger than 40 mm in diameter and located in PS Segment 4a were associated with the highest degree of technical difficulty. However, post-operative outcomes were not significantly different compared to smaller tumors or tumors located in other segments.
JOURNAL OF HEPATO-BILIARY-PANCREATIC SCIENCES
(2023)
Article
Oncology
Jian Chu, Yunzhe Li, Misi He, Hui Zhang, Lingling Yang, Muyao Yang, Jingshu Liu, Chenxi Cui, Liquan Hong, Xingchi Hu, Lei Zhou, Tangya Li, Changchun Li, Huiwen Fan, Guoqin Jiang, Tingyuan Lang
Summary: The aberrant suppression of ZSCAN1 in breast cancer indicates a poor prognosis. Ectopic expression of ZSCAN1 inhibits the proliferation, clonogenicity, and tumorigenicity of breast cancer cells. Mechanistic studies reveal that ZSCAN1 directly binds to the promoter region of WWTR1 (encodes TAZ), inhibiting its transcription and thereby suppressing the stemness of breast cancer stem cells.
FRONTIERS IN ONCOLOGY
(2023)
Article
Medicine, General & Internal
Joy Wong, Gayathri Govindasamy, Arun Prasath, William Hwang, Aloysius Ho, Sharon Yeo, Louis Tong
Summary: This study aimed to evaluate the effectiveness of umbilical cord plasma eyedrops for the treatment of recalcitrant dry eyes in a local Singaporean context. The results showed that this treatment significantly improved kerato-epitheliopathy staining scores in patients. This suggests that allogeneic plasma may be a promising form of treatment for recalcitrant dry eye.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Gastroenterology & Hepatology
Wen Hui Lim, Snow Yunni Lin, Cheng Han Ng, Darren Jun Hao Tan, Jieling Xiao, Jie Ning Yong, Phoebe Wen Lin Tay, Nicholas Syn, Yip Han Chin, Kai En Chan, Chin Meng Khoo, Nichola Chew, Roger S. Y. Foo, Asim Shabbir, Eunice X. Tan, Daniel Q. Huang, Mazen Noureddin, Arun J. Sanyal, Mohammad Shadab Siddiqui, Mark D. Muthiah
Summary: This meta-analysis compared the effects of restrictive bariatric procedures and foregut bypass on NAFLD patients. It found that both procedures significantly improved fibrosis resolution, but foregut bypass had a greater impact on fibrosis and steatosis scores. Foregut bypass also led to significant improvements in BMI and LDL compared to restrictive procedures. The study concluded that foregut bypass is a more favorable choice for managing NAFLD due to its histological and metabolic advantages.
HEPATOBILIARY SURGERY AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Veluchamy Amutha Barathi, Candice E. H. Ho, Louis Tong
Summary: Myopia is a prevalent refractive error disorder characterized by elongation of the eye. This study identified the TGM2 gene and muscarinic receptors as important factors in the development of myopia. Targeting TGM2 and blocking muscarinic receptors could potentially be beneficial in interfering with the progression of myopia.
Article
Surgery
Bobby V. M. Dasari, Dimitri Raptis, Nicholas Syn, Alejandro Serrablo, Jose Manuel Ramia, Andrea Laurenzi, Christian Sturesson, Timothy M. Pawlik, Ajith K. Siriwardena, Mickael Lesurtel, Scientific Committee of E-AHPBA
Summary: A retrospective international multicentre study was conducted to assess the clinical and pathological determinants of overall survival and recurrence-free survival among patients undergoing surgical clearance of bilobar liver metastases from colorectal cancer. The study included 1236 patients from 70 centres, majority with synchronous liver metastases. Overall survival rates at 1, 2, 3, and 5 years were 86.4%, 67.5%, 52.6%, and 33.8% respectively, with recurrence-free survival rates of 48.7%, 26.6%, 19.2%, and 10.5%. Adjuvant chemotherapy in margin-positive resections showed a survival advantage. The study developed a prognostic nomogram for overall survival with high accuracy.