Article
Biochemistry & Molecular Biology
Tomoo Suzuki, Yidan Dai, Masanori Ono, Junya Kojima, Toru Sasaki, Hiroshi Fujiwara, Naoaki Kuji, Hirotaka Nishi
Summary: Uterine leiomyomas, the most common pelvic tumors in women of reproductive age, can cause various symptoms and reproductive issues. This study found that UCHL1 expression is significantly higher in leiomyomas than in the myometrium. Inhibition of UCHL1 resulted in downregulation of COL1A1 and COL3A1 expression and decreased migration and contractility of leiomyoma cells. Targeting UCHL1 activity may be a potential therapeutic strategy for uterine leiomyoma.
Article
Immunology
Tobias Zrzavy, Romana Hoftberger, Isabella Wimmer, Thomas Berger, Paulus Rommer, Stefan Macher
Summary: Autoimmune encephalitis presents a diagnostic challenge due to its diverse clinical manifestations. A monocentric cohort study showed that some AIE patients had normal CSF results at diagnosis, highlighting the importance of antibody testing in timely diagnosis. OCB status in anti-NMDAR AIE patients may serve as a potential prognostic biomarker, but further studies are needed for validation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Obstetrics & Gynecology
Meghan C. H. Ozcan, Lisa Cruz, Morgan F. F. Woodman, Fusun Gundogan, Kathryn J. J. Grive
Summary: The unique deubiquitinating enzyme UCHL1 regulates protein turnover only in oocytes, spermatogonia, and neurons. This study investigates the variation in UCHL1 expression across fetal maturation of oocytes to assess the lifelong ovarian reserve. The study found that UCHL1 expression in oocytes increases during ovarian development and reaches a plateau at 27 weeks gestational age, maintaining elevated levels until 36 weeks. This increase in expression is correlated with the increase in oocyte area, particularly during the transition of oocytes into primordial follicles.
REPRODUCTIVE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yuan Hu, Chenyang Qi, Jiaoyu Shi, Weiqiang Tan, Zhonghua Zhao, Yanyong Xu, Huijuan Wu, Zhigang Zhang
Summary: UCHL1 deficiency leads to severe kidney damage and proteinuria in podocytes, which is associated with ER stress, protein accumulation, and cell apoptosis. UCHL1 may be a potential target for preventing non-immune complex-mediated glomerulopathy.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Infectious Diseases
Medha Tandon, Saurabh Kataria, Jenil Patel, Tejas R. Mehta, Maha Daimee, Viral Patel, Apoorv Prasad, Anisa Anila Chowdhary, Shruti Jaiswal, Shitiz Sriwastava
Summary: Limited literature exists on Cerebrospinal fluid (CSF) findings in COVID-19 patients with neurological symptoms. Analysis of 113 patients showed elevated CSF protein levels in fatal cases and higher CSF protein and cell counts in severe cases. Further studies on the pathophysiology of neurological complications in COVID-19 are recommended.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biophysics
Jinhee Lee, Bryant J. Kane, Mukund Khanwalker, Koji Sode
Summary: The incidence of traumatic brain injury (TBI) is increasing yearly, highlighting the importance of timely diagnosis for improved patient outcomes. In this study, a novel system for rapid and one-step electrochemical detection of ubiquitin C-terminal hydrolase L1 (UCHL1) was developed. The system demonstrated fast detection, no need for washing steps, and wide concentration range in artificial serum samples, making it a promising candidate for on-site TBI detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Oncology
Xiaowei Wang, Na Zhang, Meihua Li, Tao Hong, Wei Meng, Taohui Ouyang
Summary: Ubiquitin C-terminal hydrolase-L1 (UCH-L1) is a lesser-known member of the deubiquitinating enzyme family that has both deubiquitinase and ubiquitin ligase activity and plays a role in stabilizing ubiquitin. It was first discovered in the brain and is involved in regulating cell differentiation, proliferation, transcriptional regulation, and many other biological processes. UCH-L1 is predominantly expressed in the brain and has a role in tumor promotion or inhibition. The effect of UCH-L1 dysregulation in cancer and its underlying mechanisms are still debated. Extensive research into the role of UCH-L1 in different types of cancer is vital for future UCH-L1-associated cancer treatment. This review provides insights into the molecular structure and function of UCH-L1, summarizes its role in different types of cancer, and discusses how novel treatment targets contribute to cancer research.
Article
Immunology
Yu Jia, Mingyu Li, Dawei Li, Mengyao Zhang, Huifang Wang, Lidong Jiao, Zhaoyang Huang, Jing Ye, Aihua Liu, Yuping Wang
Summary: This study analyzed the characteristics of immune-mediated cerebellar ataxias (IMCAs) associated with neuronal surface antibodies (NSAbs). Compared with other forms of IMCAs, patients with anti-NSAbs-associated CAs showed more symptoms of encephalopathy, a higher rate of pleocytosis and positive oligoclonal bands in cerebrospinal fluid (CSF), and positive therapeutic effect.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medical Laboratory Technology
Julia Maroto-Garcia, Ana Martinez-Escribano, Virginia Delgado-Gil, Minerva Manez, Carmen Mugueta, Nerea Varo, Angela Garcia de la Torre, Maximiliano Ruiz-Galdon
Summary: Multiple sclerosis (MS) is the most common demyelinating disease of the central nervous system. Currently, there is no definitive cure for MS, but new therapies have been developed based on the search for new biomarkers. Diagnosis of MS relies on integrating clinical, imaging, and laboratory findings, as there is no single specific clinical feature or diagnostic laboratory biomarker. The presence of immunoglobulin G oligoclonal bands (OCB) in cerebrospinal fluid is the most commonly used laboratory test, but other biomarkers, such as kappa free light chain, may have higher sensitivity and specificity.
CLINICA CHIMICA ACTA
(2023)
Article
Neurosciences
Linrui Dong, Qingqing Chang, Jianjun Ma, Chuanze Liu, Dashuai Guo, Xiaohuan Li, Dawei Yang, Yongyan Fan, Keke Liang, Dongsheng Li, Qi Gu
Summary: This study investigates the relationship between serum UCH-L1 levels, plasma NfL levels, and cognitive dysfunction in PD patients. The results show significant differences in UCH-L1 and NfL levels among PD patients with normal cognitive function, mild cognitive impairment, and dementia. Decreased UCH-L1 levels and elevated NfL levels are associated with cognitive dysfunction in PD patients, suggesting they can be potential biomarkers for its diagnosis.
NEUROSCIENCE LETTERS
(2023)
Article
Immunology
Benjamin Vlad, Ina Reichen, Stephan Neidhart, Marc Hilty, Dimitra Lekaditi, Christine Heuer, Amanda Eisele, Mario Ziegler, Markus Reindl, Andreas Lutterotti, Axel Regeniter, Ilijas Jelcic
Summary: Basic CSF parameters vary considerably in different clinical phenotypes of MOGAD. Q(Alb)>10x10(-3) and absence of CSF-restricted OCB are highly useful to differentiate MOGAD from MS.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Fotini Boufidou, Aigli G. Vakrakou, Maria Anagnostouli, Kostas Patas, Georgios Paraskevas, Stylianos Chatzipanagiotou, Leonidas Stefanis, Maria-Eleftheria Evangelopoulos
Summary: The performance of a new quantitative marker for intrathecal IgG synthesis was evaluated and compared with established markers used for the same purpose. A retrospective analysis was conducted on a cohort of 372 patients with central nervous system demyelination, who underwent lumbar puncture and oligoclonal bands (OCBs) detection. Auer's formula had lower sensitivity compared to Reiber's formula and IgG index, possibly due to the mathematical model used. The use of Reiber's hyperbolic formula and similar formulas are recommended for discriminating between CNS-derived and blood-derived molecules in clinical laboratories.
Article
Chemistry, Multidisciplinary
Anna Sankiewicz, Tomasz Guszcz, Ewa Gorodkiewicz
Summary: The study investigates the relationship between the ubiquitin-proteasome system and transitional cell carcinoma, using SPRi-based biosensors to detect 20S proteasome and UCH-L1 in body fluids of TCC patients. The concentrations of both proteins were found to be significantly elevated in TCC patients compared to healthy subjects, suggesting their potential as cancer biomarkers.
APPLIED SCIENCES-BASEL
(2021)
Article
Medicine, General & Internal
Sawsan Abuhamdah, Tahia H. Saleem, Bakheet E. M. Elsadek, Omyma Ashraf, Ali R. Hamdan, Eslam El Sayed El-Khateeb, Saeda M. Abd Elwahab, Mohammed H. Hassan
Summary: The study aimed to assess the role of serum UCH-L1 and NGB in predicting severity and outcome of traumatic spinal cord injury (TSCI). The results showed that UCH-L1 could be a helpful marker in reflecting the degree of TSCI and predicting its outcome, while NGB needs further assessment.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2022)
Article
Cell Biology
Dan-Dan Wu, Yan-Ming Xu, De-Ju Chen, Zhan-Ling Liang, Xu-Li Chen, Machteld N. Hylkema, Marianne G. Rots, Sheng-Qing Li, Andy T. Y. Lau
Summary: Chronic exposure to cadmium can lead to a variety of diseases including cancer. By studying cadmium-transformed human lung epithelial cells, researchers identified differentially expressed proteins in five subcellular fractions and found that UCHL1 plays a crucial role in suppressing EMT. This suggests that UCHL1 could be a potential therapeutic target for chronic cadmium-induced carcinogenesis.
CELL BIOLOGY AND TOXICOLOGY
(2021)
