Article
Biochemistry & Molecular Biology
Jingqian Su, Siyuan Chen, Jianbin Xiao, Zhihua Feng, Shan Hu, Qiaofen Su, Qi Chen, Duo Chen
Summary: This study explores the therapeutic potential of aloe-emodin (AE) in the treatment of CLP-induced sepsis, demonstrating its ability to mitigate systemic inflammation and regulate the gut microbiota. The study provides novel insights into the molecular mechanisms underlying the anti-inflammatory effects of AE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Bin Zhang, Feng Zheng, Anpeng Liu, Zhen Li, Fei Zheng, Qiangsheng Liu, Liu Yang, Kai Chen, Yanlin Wang, Zongze Zhang, Qianwen He, Jia Zhan
Summary: Activation of CB2 receptors plays a protective role in sepsis by inhibiting pyroptosis and reducing the release of inflammatory mediators. The protective effect depends on the presence of endogenous CB2 receptors.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Plant Sciences
Jae-Hun Ahn, Eun-Jung Song, Do-Hyeon Jung, Yeong-Jun Kim, In-Su Seo, Seong-Chan Park, You-Seok Jung, Eun-Seo Cho, Sang Hyun Mo, Jung Joo Hong, Jeong-Yong Cho, Jong-Hwan Park
Summary: The sesquiterpene lactone estafiatin derived from Artemisia scoparia was found to have anti-inflammatory effects on LPS-stimulated macrophages and protect mice from sepsis.
Article
Toxicology
Takashi Ashino, Yuki Nakamura, Hirokazu Ohtaki, Yoichiro Iwakura, Satoshi Numazawa
Summary: In the study of polymicrobial sepsis, pro-inflammatory cytokines play a vital role in regulating hepatic drug transporter expression. The research found that both CLP and LPS can reduce the expression of drug transporters, but IL-6 is the major factor in the downregulation of hepatic multidrug transporters caused by polymicrobial sepsis.
TOXICOLOGY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Pratsanee Hiengrach, Peerapat Visitchanakun, Pakteema Tongchairawewat, Ponphisudti Tangsirisatian, Thitiphat Jungteerapanich, Patcharee Ritprajak, Dhammika Leshan Wannigama, Pattarin Tangtanatakul, Asada Leelahavanichkul
Summary: BAM15 shows potential as an adjuvant therapy against sepsis hyperinflammation by attenuating multiple aspects of septic conditions, such as survival, organ damage, apoptosis, and brain injury, as well as regulating mitochondrial functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
He Miao, Song Chen, Renyu Ding
Summary: Sepsis is a complex syndrome characterized by dysregulated host responses and multiple organ dysfunction, with underlying molecular mechanisms remaining unknown. Proteomics technology can help monitor proteome changes and identify differentially expressed proteins in sepsis, providing insights into the pathophysiological process of sepsis. This review aims to comprehensively summarize available literature on sepsis, proteomics, and related factors to offer novel perspectives on the molecular mechanisms of sepsis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Neurosciences
Cheng-Long Ge, Wei Chen, Li-Na Zhang, Yu-Hang Ai, Yu Zou, Qian-Yi Peng
Summary: Our study reveals the important role of the HPC-PFC pathway in improving cognitive dysfunction in a mouse model of CLP sepsis and provides a novel pathogenetic mechanism for SAE.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Genetics & Heredity
Karim Ullah, Yan Li, Qiaoshan Lin, Kaichao Pan, Tu Nguyen, Solanki Aniruddhsingh, Qiaozhu Su, Willard Sharp, Rongxue Wu
Summary: This study performed a comparative analysis of two widely used mouse models of septic cardiomyopathy, and identified key genes and pathways involved in its development. The two models were able to induce septic heart dysfunction within 24 hours, but they had different transcriptome expression profiles and potentially diverse pathways contributing to sepsis-induced heart failure.
Article
Biochemistry & Molecular Biology
Warerat Kaewduangduen, Peerapat Visitchanakun, Wilasinee Saisorn, Ariya Phawadee, Charintorn Manonitnantawat, Chirapas Chutimaskul, Paweena Susantitaphong, Patcharee Ritprajak, Naraporn Somboonna, Thanya Cheibchalard, Dhammika Leshan Wannigama, Patipark Kueanjinda, Asada Leelahavanichkul
Summary: Bacteria-free DNA in sepsis mice is partly derived from gut translocation, influencing systemic inflammation and exacerbating sepsis severity. Inhibition of TLR-9 can attenuate the effects of bacterial DNA in macrophages, potentially improving sepsis hyper-inflammation in some cases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Donghwan Park, MyungJa Ro, A-Jin Lee, Dong-Wook Kwak, Yunro Chung, Jae-Hong Kim
Summary: The study found that BLT2, a receptor for leukotriene B-4 with low affinity, plays a role in sepsis, a systemic inflammatory response syndrome caused by infection. Levels of BLT2 ligands and synthesizing enzymes for LTB4 and 12(S)-HETE were significantly increased in lung and liver tissues in a septic mouse model. Blocking BLT2 suppressed the production of sepsis-associated cytokines and alleviated lung inflammation in the sepsis group, suggesting that BLT2 may be a potential therapeutic target for sepsis patients.
MOLECULES AND CELLS
(2021)
Article
Physiology
Jose Maria Moreno-Navarrete, Ferran Comas, Vincent de Jager, Jose Manuel Fernandez-Real, Hjalmar R. Bouma
Summary: The study found that sepsis reduces the expression of adipogenesis-related genes in white adipose tissue while promoting inflammation in adipose tissue. In contrast, sepsis increases inflammation in brown adipose tissue without affecting the expression of thermogenic genes.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Immunology
Takashi Ashino, Yuki Nakamura, Hirokazu Ohtaki, Yoichiro Iwakura, Satoshi Numazawa
Summary: Sepsis affects drug metabolism and the expression of cytochrome P450s. The study shows that cecal ligation and puncture (CLP) induces the expression of proinflammatory cytokines and leads to decreased survival in IL-6(-/-) mice. CLP also downregulates the expression of Cyp2c and Cyp3a11 genes, but this effect is abolished by IL-6 depletion.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Critical Care Medicine
Xiancheng Chen, Xiaojing Li, Huimin Lu, Yali Xu, Yu Wei, Ke Cao, Zhanghua Zhu, Ming Chen, Wenkui Yu
Summary: PIICS poses a growing challenge in ICUs, and we established a mouse model to study the disease's pathophysiology and treatment. The model shows two mortality peaks and a protracted course of 14 days, indicating a re-aggravated status compared to previous models.
Article
Pharmacology & Pharmacy
Changnan Wang, Jihong Yuan, Jiankui Du
Summary: The study showed that resveratrol could alleviate sepsis-induced acute lung injury/acute respiratory distress syndrome by regulating mitochondrial function and mitophagy.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Immunology
Claudia V. de Araujo, Frederik Denorme, W. Zac Stephens, Qing Li, Mark J. Cody, Jacob L. Crandell, Aaron C. Petrey, Kimberly A. Queisser, John L. Rustad, Judah L. Evangelista, Michael S. Kay, Joshua D. Schiffman, Robert A. Campbell, Christian C. Yost
Summary: Neutrophil extracellular traps (NETs) can contribute to host inflammatory tissue damage during sepsis. This study investigated a novel therapeutic agent, neonatal NET-Inhibitory Factor (nNIF), and found that nNIF inhibited NET formation and improved survival in a mouse model of sepsis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Decheng Yin, Jian Fu, Ida Allabauer, Oliver Witzke, Song Rong, Andre Hoerning
Summary: The study presents a modified surgical technique for heterotopic abdominal heart transplantation in mice, using the intrathoracic inferior vena cava for anastomosis to improve success rates.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Biochemistry & Molecular Biology
Inga Soerensen-Zender, Song Rong, Hermann Haller, Roland Schmitt
Summary: This study investigated the therapeutic potential of increasing AZGP1 in treating fibrotic kidney disease. Both systemic treatment with recombinant AZGP1 and conditional overexpression of AZGP1 in proximal tubular cells showed beneficial effects, including reduced collagen deposition and improved lipid metabolism in tubular cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Wenyu Xiang, Shuai Han, Cuili Wang, Hongjun Chen, Lingling Shen, Tingting Zhu, Kai Wang, Wenjie Wei, Jing Qin, Nelli Shushakova, Song Rong, Hermann Haller, Hong Jiang, Jianghua Chen
Summary: In this study, RNA sequencing and bioinformatics methods were used to characterize the peripheral blood mononuclear cells of patients with acute renal allograft rejection. Differentially expressed genes and inflammatory pathways associated with rejection were identified, and a combination of genes was able to distinguish between rejection and non-rejection with high accuracy. This study provides a foundation for the discovery of biomarkers and a better understanding of acute renal allograft rejection.
FRONTIERS IN MEDICINE
(2022)
Article
Agriculture, Multidisciplinary
Elisabeth Koch, Nadja Kampschulte, Nils Helge Schebb
Summary: This study analyzed n3 supplements made from fish oil, krill oil, and micro-algae oil using LC-MS. The results showed significant variations in total oxylipins and non-esterified fatty acid content among the supplements, with krill oil having the highest concentration and micro-algae oil having the lowest. The study also identified terminal hydroxylation products as authenticity markers for micro-algae oil. Refined micro-algae and fish oil had low levels of non-esterified fatty acids.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Urology & Nephrology
Anne M. Huesing, Vera C. Wulfmeyer, Svenja Gaedcke, Susanne V. Fleig, Song Rong, David DeLuca, Hermann Haller, Roland Schmitt, Sibylle von Vietinghoff
Summary: The risk of cardiovascular events increases after renal ischemia-reperfusion (IR) injury. Leukocytes play a crucial role in the growth and instability of atherosclerotic plaques. This study established a model of enhanced remote atherosclerosis after renal IR injury and investigated the underlying inflammatory mechanisms.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Immunology
Lisha Teng, Lingling Shen, Wenjun Zhao, Cuili Wang, Shi Feng, Yucheng Wang, Yan Bi, Song Rong, Nelli Shushakova, Hermann Haller, Jianghua Chen, Hong Jiang
Summary: This study identifies six hub genes and functionally enriched pathways related to acute rejection (AR) in kidney transplantation, and confirms the involvement of SLAMF8 through TLR4 in the process of AR.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Shuai Han, Wenjun Zhao, Cuili Wang, Yucheng Wang, Rong Song, Hermann Haller, Hong Jiang, Jianghua Chen
Summary: Currently, kidney transplant biopsy is the main method for diagnosing acute allograft rejection, but it is invasive. By combining proteomics studies and public data sources, we found that immunoglobulin heavy constant alpha 1 and immunoglobulin kappa constant showed good performance in diagnosing acute allograft rejection and were validated through RNA sequencing.
FRONTIERS IN MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Johanna Stoermer, Wilfried Gwinner, Katja Derlin, Stephan Immenschuh, Song Rong, Mi-Sun Jang, Nelli Shushakova, Hermann Haller, Faikah Gueler, Robert Greite
Summary: This study investigated the effects of diclofenac on the progression of AKI and long-term renal consequences in the setting of pre-existing subclinical AKI. The results showed that diclofenac aggravated renal injury in a dose and time-dependent manner and even a single dose can cause progression to chronic kidney disease.
Article
Chemistry, Medicinal
Nadja Kampschulte, Tim Berking, Ibrahim E. Celik, Stefan F. Kirsch, Nils Helge Schebb
Summary: In this study, the structure-activity relationship of the inhibition of fatty acid-oxidizing cytochrome P450 monooxygenase by flavonoids was investigated. A potent and selective compound, 4'-trifluoromethylflavone, was identified as an inhibitor of 20-HETE formation.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Decheng Yin, Jian Fu, Rongjun Chen, Nelli Shushakova, Ida Allabauer, Xin-Yi Wei, Mario Schiffer, Diana Dudziak, Song Rong, Andre Hoerning
Summary: This article introduces the key steps and surgical caveats of kidney transplantation in mice, as well as important modifications to the conventional procedure. The modifications include reducing the risk of ureter necrosis and urinary tract occlusion, flexibly adjusting the size of the vascular anastomosis, and using a non-traumatic technique for donor ureter and recipient bladder anastomosis.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Biochemistry & Molecular Biology
Robert Greite, Li Wang, Lukas Gohlke, Sebastian Schott, Kirill Kreimann, Julian Doricic, Andreas Leffler, Igor Tudorache, Jawad Salman, Ruslan Natanov, Fabio Ius, Christine Fegbeutel, Axel Haverich, Ralf Lichtinghagen, Rongjun Chen, Song Rong, Hermann Haller, Vijith Vijayan, Magnus Gram, Irina Scheffner, Faikah Gueler, Wilfried Gwinner, Stephan Immenschuh
Summary: This study investigated the levels of CFH and CFH-binding hemoglobin in LuTx patients and found that CFH may be associated with postoperative AKI. Moreover, experimental studies on a mouse model of mild renal ischemia reperfusion injury confirmed the role of CFH in promoting AKI. Targeting CFH may be a therapeutic option to prevent AKI after LuTx.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Elisabeth Koch, Michelle Wiebel, Ariane Loewen, Ina Willenberg, Nils Helge Schebb
Summary: Enzymatic and nonenzymatic oxidation of linoleic (LA) and alpha-linolenic acid (ALA) during pressing and storage of plant oils leads to the formation of various oxylipins. The concentrations of oxylipins are found to be correlated with the peroxide value (PV) of the oils, while secondary volatile aldehydes do not show similar correlations. Comprehensive analysis of different types of hydroxy-, epoxy-, and dihydroxy-LA/-ALA provides new insights into the composition of plant oils and ongoing oxidation processes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Elisabeth Koch, Mustafa Bagci, Michael Kuhn, Nicole M. Hartung, Malwina Mainka, Katharina M. Rund, Nils Helge Schebb
Summary: In this study, a rapid gas chromatographic-mass spectrometry method was developed for the separation and quantification of oxysterols bearing hydroxy, epoxy, and dihydroxy groups. The method allows for precise analysis of the oxysterols, and was used to investigate oxysterol formation during oxidative stress in HepG2 cells.
Article
Biochemical Research Methods
Nicole M. Hartung, Malwina Mainka, Rebecca Pfaff, Michael Kuhn, Sebastian Biernacki, Lilli Zinnert, Nils Helge Schebb
Summary: This study describes a quantitative multi-omics approach combining liquid chromatography tandem mass spectrometry-based targeted oxylipin metabolomics and proteomics. It enables the simultaneous quantitative analysis of human COX and LOX pathway enzymes and 239 oxylipins. Using this sensitive multi-omics approach, the researchers comprehensively characterized oxylipin and protein patterns in human monocytic cell line THP-1 and differently polarized primary macrophages, demonstrating its usefulness in studying molecular modes of action involved in the modulation of the ARA cascade.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Elisabeth Koch, Ariane Loewen, Sharline Nikolay, Ina Willenberg, Nils Helge Schebb
Summary: New parameters related to the condition of frying oils were discovered by studying the oxidation products of fatty acids during deep-frying.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)