Article
Immunology
Mahreen Nawaz, Asad Ullah, Alhanouf Al-Harbi, Mahboob Ul Haq, Alaa R. Hameed, Sajjad Ahmad, Aamir Aziz, Khadija Raziq, Saifullah Khan, Muhammad Irfan, Riaz Muhammad
Summary: This study aimed to develop a broad-spectrum, multi-epitope vaccine to control bacterial infections and reduce the burden on healthcare systems. A computational framework was used to filter immunogenic vaccine candidates, and the selected candidates were further optimized and analyzed through molecular dynamics simulations. The results showed that the vaccine candidates had strong binding potential with immune receptors and were exposed to the host's immune system.
Article
Biochemistry & Molecular Biology
Doaa M. AlEraky, Hatem M. Abuohashish, Amr S. Bugshan, Maha M. Abdelsalam, Hussain A. AlHawaj, Taleb T. AlKhamis, Fatimah A. AlDossary, Nabras M. Alrayes, Yasser M. Ragab, Zeinab AbdelKhalek, Omneya M. Helmy, Mohammed A. Ramadan
Summary: This study aimed to identify antigenic peptides in H. pylori and evaluate their effectiveness as potential vaccine candidates. The results showed that the SabA antigen was particularly effective in inducing an immune response against H. pylori in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tohid Piri-Gharaghie, Abbas Doosti, Seyed Abbas Mirzaei
Summary: By using subtractive proteomics and reverse vaccinology approaches, suitable vaccine candidates against multidrug-resistant Acinetobacter baumannii were predicted. The Hcp and OmpC proteins were identified as potential vaccine constructs based on their immunogenicity and physicochemical properties. The in silico study found that the Hcp and OmpC dual vaccine construct has high specificity and is capable of inducing a significant humoral and cellular immune response.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Lu Liu, Wanli Yu, Kuojun Cai, Siyuan Ma, Yanfeng Wang, Yuhui Ma, Hongqiong Zhao
Summary: Through pangenome analysis and reverse vaccinology, 16 potential vaccine candidates for R. equi were identified, and antigenic epitopes with strong immunogenicity were predicted, providing a foundation for the development of a multivalent or universal vaccine.
Article
Biochemistry & Molecular Biology
Joana Couto, Goncalo Seixas, Christian Stutzer, Nicholas A. Olivier, Christine Maritz-Olivier, Sandra Antunes, Ana Domingos
Summary: In the study, reverse vaccinology approaches were used to identify potential vaccine candidates from Rhipicephalus bursa proteins, with nine candidates selected and three peptides chosen as potential vaccine targets. By combining in silico methods with in vivo immunogenicity evaluation, this study demonstrated the potential of these peptides as anti-tick vaccines prior to costly infestation studies on ovine hosts.
Article
Immunology
Ana Clara Gazzinelli-Guimaraes, Denise Silva Nogueira, Chiara Cassia Oliveira Amorim, Fabricio Marcus Silva Oliveira, Anderson Coqueiro-Dos-Santos, Samuel Alexandre Pimenta Carvalho, Lucas Kraemer, Fernando Sergio Barbosa, Vanessa Gomes Fraga, Flaviane Vieira Santos, Joseane Camilla de Castro, Remo Castro Russo, Milena Apetito Akamatsu, Paulo Lee Ho, Maria Elena Bottazzi, Peter J. Hotez, Bin Zhan, Daniella Castanheira Bartholomeu, Lilian Lacerda Bueno, Ricardo Toshio Fujiwara
Summary: Control of human ascariasis relies on mass drug administration, but a vaccine should be the primary target for infection control. A multipeptide chimera vaccine, ASCVac-1, demonstrated significant efficacy in reducing parasite count in a mouse model through strong IgG-based Th2 immune responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Abolfazl Hajialibeigi, Jafar Amani, Seyed Latif Mousavi Gargari
Summary: In this study, new vaccine candidates against Shigella flexneri were identified and experimentally evaluated through reverse vaccinology approach. The results demonstrate the potential of the identified antigens for vaccine development against shigellosis and support the use of reverse vaccinology for discovering novel effective antigens.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Asad Ullah, Mahboob ul Haq, Madiha Iqbal, Muhammad Irfan, Saifullah Khan, Riaz Muhammad, Amin Ullah, Muhammad Khurram, Metab Alharbi, Abdullah F. F. Alasmari, Sajjad Ahmad
Summary: Moraxella lacunata is an emerging gram-negative bacterium responsible for multiple nosocomial infections. A multi-epitope-based vaccine has been designed for M. lacunata using fusion of selected epitopes and cholera toxin-B adjuvant. Docking analysis and molecular dynamics simulation showed strong interactions and stable binding conformation between the vaccine and immune cell receptors. Theoretical binding free energies were estimated using MM-PBSA and MM-GBSA methods.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biology
Anjali Lathwal, Rajesh Kumar, Gajendra P. S. Raghava
Summary: This study developed subunit vaccine candidates against lung cancer-causing oncogenic viruses using a reverse vaccinology approach. Through systematic analysis of protein components from nine oncogenic virus species, 125 best antigenic epitopes were identified with predicted B-cell, T-cell, and/or MHC-binding capability, as well as vaccine adjuvant potential. These epitopes show promising immunogenic potential for future development as vaccines against lung cancer-causing viruses.
COMPUTERS IN BIOLOGY AND MEDICINE
(2021)
Review
Immunology
Kanwal Khalid, Chit Laa Poh
Summary: The clinical use of antibiotics has led to the emergence of multi-drug resistant bacteria, resulting in the current antibiotic resistance crisis. To address this issue, next-generation vaccines are being developed to prevent antimicrobial resistance caused by multi-drug resistant bacteria. Traditional vaccine platforms, such as inactivated vaccines and live attenuated vaccines, have shown reduced efficacy against emerging antibiotic-resistant bacteria, including multi-drug resistant M. tuberculosis.
Article
Veterinary Sciences
Xiaodong Zai, Ying Yin, Fengyu Guo, Qiaoling Yang, Ruihua Li, Yaohui Li, Jun Zhang, Junjie Xu, Wei Chen
Summary: This study utilized core proteome analysis and a compositive RV methodology to screen potential broad-spectrum antigens against Brucella spp. Thirty-two candidate antigens were identified, and three proteins with a high degree of conservation were selected for assessment of immunogenicity and preliminary protection in a mouse model. These antigens induced specific humoral and cellular immune responses, reducing organ bacterial load and providing varying degrees of protection. Further evaluation is needed to determine the levels of protection conferred by the vaccine antigens against wild-type pathogenic Brucella species challenge.
VETERINARY RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Syed Aun Muhammad, Jinlei Guo, Komal Noor, Aymen Mustafa, Anam Amjad, Baogang Bai
Summary: Through immunoproteomics, potential vaccine candidates for NiV have been predicted, which can activate the host immune system to prevent NiV infection.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Fisheries
Basmeet Kaur, Naveen B. T. Kumar, Anuj Tyagi, Shanthanagouda Admane Holeyappa, Niraj Kumar Singh
Summary: The study demonstrated that the whole-cell A. hydrophila biofilm vaccine could protect fish against infections from different geographical isolates. Six protective proteins and fourteen potential vaccine candidates were identified through the research, providing valuable insights for developing vaccines against A. hydrophila pathogens.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ilhem Meniai, Alexandre Thibodeau, Sylvain Quessy, Valeria R. Parreira, Philippe Fravalo, Guy Beauchamp, Marie-Lou Gaucher
Summary: The study identified potential immunogenic proteins of C. perfringens strains through genomic analysis, resulting in the selection of 12 candidate proteins, with 6 showing higher antigenic potential and possible association with the virulence of C. perfringens.
Article
Microbiology
Jie Li, Jingxuan Qiu, Zhiqiang Huang, Tao Liu, Jing Pan, Qi Zhang, Qing Liu
Summary: Salmonella is a major foodborne pathogen, and vaccination is the most effective means to combat its infections. In this study, reverse vaccinology was used to identify vaccine candidates with high immunogenicity and protective immunity. The results showed that some proteins screened in the study could serve as good candidates for broad-spectrum vaccines.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Orthopedics
Badaruddin Sahito, Sheikh Muhammad Ebad Ali, Sofia Fatima Farooqui, Awais Abro, Javed Ahmed, Younis
Summary: This retrospective comparative study examined the effects of neoadjuvant denosumab on the functional outcomes, recurrence rate, and complications in patients who underwent arthrodesis of the shoulder after resection of grade III giant cell tumors (GCT) of the proximal humerus. The study found no significant differences between the denosumab and non-denosumab groups in terms of functional outcomes, recurrence, and complications. However, patients in the denosumab group had lower pain scores at 6 weeks postoperatively.
EUROPEAN JOURNAL OF ORTHOPAEDIC SURGERY AND TRAUMATOLOGY
(2023)
Review
Chemistry, Inorganic & Nuclear
Tanzeela Anis, Syed Mujtaba ul Hassan, Ahmat Khurshid, M. Fakhar-e-Alam, Faisal Shahzad, A. Ali, Jamil Ahmad, Nazia Hossain
Summary: This review provides a comprehensive overview of hollow, multifunctional structures for biomedical applications, with a focus on the use of nanotechnology and its unique prospects in personalized treatment, multimodal imaging, multimodal therapy, and targeted drug delivery. The performance of different materials and synthesis strategies are discussed, and the development of more sophisticated systems in the future is anticipated.
JOURNAL OF CLUSTER SCIENCE
(2023)
Article
Agronomy
Muhammad Abdullah, Muhammad Zulkiffal, Tabussam Tufail, Farah Naz Akbar, Muhammad Owais, Javed Ahmed, Ahsan Javed, Muhammad Ijaz Tabassum, Amna Kanwal, Muhammad Asghar, Muhammad Musa, M. A. Rehman Arif, Amir Hameed, Iftikhar Haider
Summary: This study aimed to incorporate barley flour into whole wheat flour to develop chapatti. Different samples of flour blend were made by adding barley at different rates, and their proximate analyses and quality variables were determined. The results showed that the addition of barley increased protein, ash, energy, and beta-glucan contents, while decreasing gluten, carbohydrates, and phytates. Similar trends were observed in the characteristics of chapatti. Sensory evaluation indicated that chapatti with 20% barley incorporation had good nutritional quality and acceptability.
CEREAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Swati Verma, Anupinder Singh, Sandeep Sharma, Paramjit Kaur, Sachin Kumar Godara, Parambir Singh Malhi, Jahangeer Ahmed, P. D. Babu, Mandeep Singh
Summary: Polycrystalline samples of M-type hexaferrite with varying chemical composition were successfully synthesized using solid state reaction technique. The structural, microstructural and magnetic properties of these samples were investigated. The results showed good phase purity, decreased crystallite size and increased cell volume with increasing dopant concentration. Furthermore, the hexaferrite samples exhibited excellent magnetic properties, making them suitable for various applications.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Article
Biochemistry & Molecular Biology
Javed Ahmed, Mohsin Abbas Khan, Muhammad Ehsan Khalid, Irshad Ahmad, Irfan Pervaiz, Umair Khurshid, Saharish Khaliq, Kashif ur Rehman Khan, Muhammad Adeel Arshad, Ghadeer M. M. Albadrani, Ahmed E. E. Altyar, Amany A. Sayed, Mousa O. Germoush, Mohamed M. Abdel-Daim
Summary: The study synthesized and characterized different metformin derivatives with potent antidiabetic potential and minimal side-effects. Metal complexes containing Cu II and Zn II were generated using aromatic aldehydes and ketones, and characterized through various techniques. The derivatives showed significant antidiabetic activities against targeted enzymes and hold promise for future study and development.
Article
Surgery
Ceranic Miljan, Ahmed Gendia, Masood U. R. Rehman, Djukanovic Blagoje, Janic Mladen, Krdzic Igor, Stojakovic Nebojsa, Gluhovic Aleksandar, Loncar Zlatibor, Jamil Ahmed, Parvaiz Amjad
Summary: The Serbian National Training Programme for minimally invasive colorectal surgery (LapSerb) aimed to establish laparoscopic colorectal surgery across Serbia. This study reports the outcomes of laparoscopic colorectal resection performed by LapSerb certified surgeons.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2023)
Review
Chemistry, Physical
Sanjay Kumar, Himanshi, Jyoti Prakash, Ankit Verma, Suman, Rohit Jasrotia, Abhishek Kandwal, Ritesh Verma, Sachin Kumar Godara, M. A. Majeed Khan, Saad. M. M. Alshehri, Jahangeer Ahmed
Summary: Graphene-based materials, known for their remarkable electrical, mechanical, and optical traits, have gained significant scientific interest in modern technology research. In this review paper, different synthesis methods for extracting graphene and its derivatives are discussed, along with their structural, mechanical, and optical characteristics. The application of graphene-based composites in environmental purification techniques such as filtration, adsorption, and photocatalysis is extensively reviewed, highlighting the challenges and opportunities for advanced environmental applications.
Review
Neurosciences
Rabea Basri, Faryal Mehwish Awan, Burton B. B. Yang, Usman Ayub Awan, Ayesha Obaid, Anam Naz, Aqsa Ikram, Suliman Khan, Ijaz ul Haq, Sadiq Noor Khan, Muslim Bin Aqeel
Summary: Regulating autophagy, a cellular waste-recycling system, in the brain is crucial for preventing neurodegeneration. Dysfunction in autophagy can lead to neuronal death. Evidence suggests that circular RNAs (circRNAs) play a role in autophagosome assembly, but the regulatory networks involving circRNAs, autophagy, and neurodegeneration are not well understood. Understanding these interactions could provide valuable resources for the diagnosis and therapy of neurodegenerative diseases. Future research on brain-protective mechanisms of autophagy associated circRNAs may shed light on the biology of the nervous system and lead to new approaches for treating these diseases.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Engineering, Electrical & Electronic
Sachin Kumar Godara, Sher Singh Meena, Rohit Jasrotia, Jyoti Prakash, Ankit Verma, Ranjit Singh, A. K. Srivastava, Mandeep Singh, Pradip K. Maji, Ankit Jain, Ashwani Kumar Sood, Jahangeer Ahmed, Saad M. Alshehri, Amir Mehtab, Tokeer Ahmad, Aslam Hossain, Abhishek Kandwal
Summary: In this study, M-type hexaferrites Ba0.5Ca0.5ZnxZrxFe12-2xO19 were fabricated using the sol-gel technique. The exchange of Fe3+ ions by Zn2+-Zr4+ ions was confirmed by powder X-ray diffraction patterns. The lattice parameters 'a' and 'c' increased as the doping level increased. Scanning electron microscopy images showed a slight decrease in grain size with doping. The highest saturation magnetization was observed for the x = 0.2 configuration. The coercivity decreased significantly with Zn2+-Zr4+ doping. Mossbauer spectra revealed the preferential site occupancy by dopant ions and the ionic state of Fe-ions.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
(2023)
Article
Chemistry, Physical
Nisha Yadav, Shivani Shiva, Preeti Oswal, Arun Kumar, Ajai Kumar Singh, Balvinder Singh, Bidhan Pandit, Jahangeer Ahmed, Gyandshwar Kumar Rao
Summary: The paper presented the role of ligands in stabilizing colloidal silver-quantum dots (Ag-QDs) and their applications in colorimetric detection of metal ions/anions. The Ag-QDs were also used for reducing nitroaromatics and showed selective colorimetric sensing of Hg(II)/Cu(II) and I- ions. The results suggest that the ligands with donor atoms effectively stabilize the Ag-QDs and the pendant alkyl chain governs their dispersion, while the colorimetric change is attributed to ion-mediated aggregation of QDs.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Engineering, Electrical & Electronic
Pargam Vashishtha, Manoj Kumar, Pukhraj Prajapat, Jahangeer Ahmed, Vidya Nand Singh, Govind Gupta
Summary: By utilizing ultraviolet-responsive gallium nitride (GaN) and infrared-activated tin selenide (SnSe), a broadband heterojunction-based optical detector was fabricated, combining the detection capabilities of GaN in the ultraviolet to visible range and SnSe in the visible to shortwave-infrared range. The detector exhibited high responsivity, low dark current, and edge contact geometry for direct access to the heterostructure interface of both materials. The device showed excellent performance across the ultraviolet-C to shortwave-infrared region, with a maximum responsivity of 128 AW-1, a noise equivalent power of 5.2 x 10-14 WHz- 1/2, and a quantum efficiency of 5 x 104% for ultraviolet illumination, and a maximum responsivity of 6.06 AW-1 and noise equivalent power of 1 x 10-12 WHz- 1 for infrared illumination. This research opens up new possibilities for wideband detection in optoelectronic applications utilizing heterostructure-based devices.
MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING
(2023)
Article
Multidisciplinary Sciences
Hassan Ayaz, Nouman Aslam, Faryal Mehwish Awan, Rabea Basri, Bisma Rauff, Badr Alzahrani, Muhammad Arif, Aqsa Ikram, Ayesha B. Obaid, Anam Naz, Sadiq Noor Khan, Burton B. Yang, Azhar Nazir
Summary: Non-coding RNAs (ncRNAs), including circular RNAs (circRNAs), play important roles in genetic information control, RNA stability, and epigenetic modifications. This study aimed to explore the regulatory networks driven by SARS-CoV-2 related circRNAs, miRNAs, and mRNAs to identify potential therapeutic options. The analysis identified a circRNA-miRNA-mRNA network and suggested that two differentially expressed circRNAs could serve as potential theranostic agents for SARS-CoV-2 infection. Overall, this study sheds light on the functional role of circRNAs as ceRNAs during SARS-CoV-2 infection.
Article
Genetics & Heredity
Cristina Skrypnyk, Aseel Ahmed Husain, Hisham Y. Hassan, Jameel Ahmed, Abdulla Darwish, Latifa Almusalam, Noureddine Ben Khalaf, Fahad Al Qashar
Summary: This study reports two Arab patients from consanguineous families diagnosed with different severities of nemaline myopathy. The clinical phenotype was linked to homozygous variants in NEB and KLHL40 genes. The study highlights the importance of detailed assessment in prenatal, neonatal, and infancy stages for accurate diagnosis and intervention of nemaline myopathy.
FRONTIERS IN GENETICS
(2023)
Article
Green & Sustainable Science & Technology
Mahvish Aslam, Imran Shafi, Jamil Ahmed, Mirtha Silvana Garat de Marin, Emmanuel Soriano Flores, Marco Antonio Rojo Gutierrez, Imran Ashraf
Summary: This research paper examines the impact of innovative HRM practices on firm performance, using four different models to analyze small and medium enterprises in a country. The results show that most innovative HRM practices have a significant impact on labor productivity, product innovation, process innovation, and marketing innovation. These findings suggest that SMEs can benefit from implementing innovative HRM practices to achieve future growth potential.
Article
Materials Science, Multidisciplinary
Khawaja Adeel Tariq, Jamil Ahmad, Syed Ali Husnain, Muhammad Sufyan Ijaz
Summary: This research investigates the use of environment friendly materials, such as coir fibers, polypropylene fibers, and jute fibers, in concrete. By reducing the diameter of steel bars using fiber ropes, an economical reinforced concrete is produced. The study examines the compressive and flexural strength of fiber-reinforced concrete with the combination of polypropylene fiber, jute fiber ropes, and steel bars. The results show that the addition of fibers enhances the properties of concrete, increasing the flexural strength and compressive strength by 6% and 7% respectively in coir concrete with fiber ropes.
JOURNAL OF THE MECHANICAL BEHAVIOR OF MATERIALS
(2023)
