Article
Biochemistry & Molecular Biology
Haiyan Cai, Parvathy Sasikumar, Gemma Little, Dominique Bihan, Samir W. Hamaia, Aiwu Zhou, Jonathan M. Gibbins, Richard W. Farndale
Summary: Research revealed that HSP47 primarily binds to a few GXR motifs in collagen, with most binding sites located near the N-terminal part of the triple-helical region. Molecular modelling and binding energy calculation showed that residues flanking the key Arg in collagen play a crucial role in defining the high-affinity HSP47 binding sites.
Review
Medicine, Research & Experimental
Eslam E. Abd El-Fattah, Amr Y. Zakaria
Summary: This article discusses the impact of heat shock proteins HSP70 and HSP47 on liver fibrosis, with HSP70 reducing protein aggregation and HSP47 promoting collagen synthesis. Additionally, the article explores potential therapeutic approaches targeting HSP70 and HSP47 as drug candidates for treating liver fibrosis.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Jie Wang, Mei Bai, Cui Zhang, Ning An, Li Wan, Xiao-ning Wang, Rong-hui Du, Yan Shen, Zhi-yao Yuan, Xu-dong Wu, Xue-feng Wu, Qiang Xu
Summary: This study investigated the therapeutic potential and underlying mechanisms of fraxinellone, a degraded limonoid, in the treatment of intestinal fibrosis. The results showed that fraxinellone administration alleviated intestinal impairments and reduced the production of fibrosis biomarkers and cytokines. In vitro experiments demonstrated that fraxinellone relieved TGF-β-induced fibrosis responses by inhibiting the TGF-β/Smad2/3 signaling pathway.
ACTA PHARMACOLOGICA SINICA
(2023)
Review
Biochemistry & Molecular Biology
Nicholas Willumsen, Christina Jensen, George Green, Neel Nissen, Jaclyn Neely, David M. Nelson, Rasmus S. Pedersen, Peder Frederiksen, Inna M. Chen, Mogens K. Boisen, Astrid Z. Johansen, Daniel H. Madsen, Inge Marie Svane, Allan Lipton, Kim Leitzel, Suhail M. Ali, Janine T. Erler, Daan P. Hurkmans, Ron H. J. Mathijssen, Joachim Aerts, Mohammed Eslam, Jacob George, Claus Christiansen, Mina J. Bissel, Morten A. Karsdal
Summary: Due to fibroblast activation into cancer-associated fibroblasts, there is increased tumor fibrosis. Measuring collagen deposition can predict prognosis and guide future drug development.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Joana Alpoim-Moreira, Carina Fernandes, Maria Rosa Rebordao, Ana Luisa Costa, Miguel Bliebernicht, Telmo Nunes, Anna Szostek-Mioduchowska, Dariusz J. Skarzynski, Graca Ferreira-Dias
Summary: This study aimed to identify possible blood biomarkers for endometrosis diagnosis and fertility assessment on mares. The results suggest that serum COL3 concentration might be considered as a potential aid for the diagnosis of endometrosis and fertility prognosis in the mare. In contrast, COL1 and hydroxyproline did not prove to be effective as biomarkers of endometrial fibrosis in this species.
Article
Biochemistry & Molecular Biology
Alexandra Louise Moller, Stefanie Thoeni, Felix Keller, Samir Sharifli, Daniel Guldager Kring Rasmussen, Federica Genovese, Morten Asser Karsdal, Gert Mayer
Summary: This study investigated the effect of combination therapy of renin-angiotensin system inhibition and sodium-glucose co-transporter-2 inhibitors on endotrophin levels in individuals with type 2 diabetes. The results showed that combination therapy led to a significant reduction in endotrophin levels and a lower risk of kidney disease progression.
Article
Biochemistry & Molecular Biology
Nadja Sparding, Michaela Neprasova, Dita Maixnerova, Federica Genovese, Morten Asser Karsdal, Marek Kollar, Helena Koprivova, Zdenka Hruskova, Vladimir Tesar
Summary: Kidney fibrosis, a characteristic of chronic kidney disease, can be assessed by measuring different biomarkers of collagen type III. In this study, three collagen type III biomarkers were measured in serum and urine samples of IgA nephropathy patients. The results showed that these biomarkers provided different information about fibrotic burden and had different correlations with the degree of fibrosis in kidney biopsies. Measuring different fragments of the same ECM protein in different matrices can provide valuable insights into pathological kidney tissue alterations in patients with IgA nephropathy.
Article
Medicine, General & Internal
Mitchel J. R. Ruigrok, Khaled E. M. El Amasi, Diana J. Leeming, Jannie M. B. Sand, Henderik W. Frijlink, Wouter L. J. Hinrichs, Peter Olinga
Summary: IPF is a chronic lung disease with an urgent need for more effective drugs, with HSP47 identified as a potential therapeutic target. Targeted siRNA therapy against Serpinh1 showed reduction in fibronectin secretion in fibrotic lung slices, indicating a promising approach for fibrosis treatment.
FRONTIERS IN MEDICINE
(2021)
Article
Multidisciplinary Sciences
Xuexiang Han, Ningqiang Gong, Lulu Xue, Margaret M. Billingsley, Rakan El-Mayta, Sarah J. Shepherd, Mohamad-Gabriel Alameh, Drew Weissman, Michael J. Mitchell
Summary: By synthesizing AA-lipidoids with high delivery efficiency and selectivity, and validating in a preclinical model of liver fibrosis, the potential of AA-lipidoids for targeted RNA delivery to activated fibroblasts has been demonstrated.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Anne Linde Mak, Jenny Lee, Anne-Marieke van Dijk, Yasaman Vali, Guruprasad P. Aithal, Joern M. Schattenberg, Quentin M. Anstee, M. Julia Brosnan, Mohammad Hadi Zafarmand, Dewkoemar Ramsoekh, Stephen A. Harrison, Max Nieuwdorp, Patrick M. Bossuyt, Adriaan G. Holleboom
Summary: This study summarized the performance of Pro-C3 in detecting significant fibrosis and advanced fibrosis in patients with NAFLD, and concluded that Pro-C3 is an important candidate biomarker for non-invasive assessment of liver fibrosis.
Article
Cardiac & Cardiovascular Systems
Tianlin He, Michaela Mischak, Andrew L. Clark, Ross T. Campbell, Christian Delles, Javier Diez, Gerasimos Filippatos, Alexandre Mebazaa, John J. McMurray, Arantxa Gonzalez, Julia Raad, Rafael Stroggilos, Helle S. Bosselmann, Archie Campbell, Shona M. Kerr, Colette E. Jackson, Jane A. Cannon, Morten Schou, Nicolas Girerd, Patrick Rossignol, Alex McConnachie, Kasper Rossing, Joost P. Schanstra, Faiez Zannad, Antonia Vlahou, William Mullen, Vera Jankowski, Harald Mischak, Zhenyu Zhang, Jan A. Staessen, Agnieszka Latosinska
Summary: This study investigated the association between urinary peptides and different subtypes of heart failure, finding that urinary peptides associated with heart failure mainly reflect changes in collagen turnover and inflammation. Patients with heart failure, regardless of ejection fraction subtype, showed differences in urinary peptide abundance. These peptides could serve as useful tools for early detection, prognosis, and predicting therapeutic response in heart failure patients.
EUROPEAN JOURNAL OF HEART FAILURE
(2021)
Article
Biochemistry & Molecular Biology
Alexandra Louise Moller, Ninna Hahn Tougaard, Daniel Guldager Kring Rasmussen, Federica Genovese, Pernille Falberg Ronn, Tine Willum Hansen, Morten Asser Karsdal, Peter Rossing
Summary: The pro-fibrotic molecule endotrophin, generated during collagen type VI formation, has been identified as a potential risk marker for complications in type 1 diabetes. Higher levels of serum endotrophin were associated with kidney complications and increased mortality in type 1 diabetes patients. However, there was no significant association between serum endotrophin and cardiovascular events or diabetic eye disease. Urinary endotrophin was not found to be associated with any outcomes.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Retraction
Chemistry, Multidisciplinary
Essak S. Khan, Shrikrishnan Sankaran, Julieta I. Paez, Christina Muth, Mitchell K. L. Han, Aranzazu del Campo
Summary: This article, published online on May 3, 2019, has been retracted due to concerns raised by a third party and subsequent investigation by the authors. The data in Figure 4 and Figure 5 could not be reproduced in follow-up experiments, rendering the associated conclusions invalid.
Article
Biology
Christopher J. Brereton, Liudi Yao, Elizabeth R. Davies, Yilu Zhou, Milica Vukmirovic, Joseph A. Bell, Siyuan Wang, Robert A. Ridley, Lareb S. N. Dean, Orestis G. Andriotis, Franco Conforti, Lennart Brewitz, Soran Mohammed, Timothy Wallis, Ali Tavassoli, Rob M. Ewing, Aiman Alzetani, Benjamin G. Marshall, Sophie Fletcher, Philipp J. Thurner, Aurelie Fabre, Naftali Kaminski, Luca Richeldi, Atul Bhaskar, Christopher J. Schofield, Matthew Loxham, Donna E. Davies, Yihua Wang, Mark G. Jones
Summary: This study provides evidence that the activation of the hypoxia-inducible factor (HIF) pathway is a critical regulator of pathogenetic collagen structure-function in fibrosis. Oxidative stress can drive the activation of pseudohypoxic HIF pathway, which alters collagen nanostructure and increases tissue stiffness.
Article
Cardiac & Cardiovascular Systems
Daniel Guldager Kring Rasmussen, Michael K. Hansen, Joseph Blair, Timothy A. Jatkoe, Bruce Neal, Morten A. Karsdal, Federica Genovese
Summary: It was found in this study that endotrophin (ETP) generated during the formation of collagen type VI predicts cardiovascular, kidney, and mortality outcomes in patients with type 2 diabetes. Therefore, ETP may be used for patient enrichment in future clinical trials.
CARDIOVASCULAR DIABETOLOGY
(2022)