期刊
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
卷 68, 期 -, 页码 946-949出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1744309112026899
关键词
AdpA; Streptomyces griseus; A-factor regulatory cascade; DNA-binding domain
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan
Streptomyces griseus AdpA is the central transcription factor in the A-factor regulatory cascade and activates a number of genes that are required for both secondary metabolism and morphological differentiation, leading to the onset of streptomycin biosynthesis as well as aerial mycelium formation and sporulation. The DNA-binding domain of AdpA consists of two helixturnhelix DNA-binding motifs and shows low nucleotide-sequence specificity. To reveal the molecular basis of the low nucleotide-sequence specificity, an attempt was made to obtain cocrystals of the DNA-binding domain of AdpA and several kinds of duplex DNA. The best diffracting crystal was obtained using a 14-mer duplex DNA with two-nucleotide overhangs at the 5'-ends. The crystal diffracted X-rays to 2.8 angstrom resolution and belonged to space group C2221, with unit-cell parameters a = 76.86, b = 100.96, c = 101.25 angstrom. The Matthews coefficient (VM = 3.71 angstrom 3 Da-1) indicated that the crystal was most likely to contain one DNA-binding domain of AdpA and one duplex DNA in the asymmetric unit, with a solvent content of 66.8%.
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