Article
Plant Sciences
Yufan Chen, Mingfa Lv, Zhibin Liang, Zhiqing Liu, Jianuan Zhou, Lian-Hui Zhang
Summary: In this study, researchers identified two c-di-GMP receptor proteins, YcgR and BcsA, in the bacterial pathogen Dickeya oryzae. They found that these proteins play a critical role in regulating bacterial motility, biofilm formation, and virulence. This study fills a gap in our understanding of c-di-GMP modulation of bacterial pathogenicity and provides valuable insights for further research on the virulence regulatory mechanisms of this important plant pathogen.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Multidisciplinary Sciences
Xiaoli Zeng, Min Huang, Qing-Xue Sun, Ye-Jun Peng, Xiaomei Xu, Yun-Bin Tang, Ju-Yuan Zhang, Yiling Yang, Cheng-Cai Zhang
Summary: This study identified a c-di-GMP receptor called CdgR, which plays a crucial role in regulating cell size in cyanobacteria. CdgR interacts with a global transcription factor DevH and functions through c-di-GMP-mediated signaling. The findings provide insights into the mechanism of c-di-GMP signaling in controlling bacterial cell size.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Immunology
Avinash Singh, Jerilyn R. Izac, Edward J. A. Schuler, Dhara T. Patel, Christopher Davies, Richard T. Marconi
Summary: This study presents the crystal structure of the Borreliella burgdorferi PlzA protein complexed with c-di-GMP for the first time, revealing the c-di-GMP binding site formed by two structurally similar domains. Additionally, the research findings indicate that the apo and holo forms of PlzA exhibit distinct structural differences.
PATHOGENS AND DISEASE
(2021)
Article
Biotechnology & Applied Microbiology
Kaihong Bai, Xiaoli Xu, Xudong Wang, Yao Li, Chengxuan Yu, Na Jiang, Jianqiang Li, Laixin Luo
Summary: This study used RNA-seq technology to investigate the mechanism of VBNC state induced by copper ion in Xcc. The results showed significant changes in gene expression and enriched metabolism-related pathways in different VBNC stages. Genes associated with cell motility were down-regulated, while pathogenicity related genes were up-regulated. This study not only summarized the related pathways of VBNC state, but also provided new ideas for studying gene expression profiles in different survival states under stress.
Article
Microbiology
Riccardo Fontana, Anna Caproni, Raissa Buzzi, Mariaconcetta Sicurella, Mattia Buratto, Francesca Salvatori, Mariangela Pappada, Stefano Manfredini, Anna Baldisserotto, Peggy Marconi
Summary: The study investigated the antibacterial effects of Moringa oleifera Lam. leaf extracts on Xanthomonas campestris pv. campestris, showing that bioactive compounds in the extracts can alter membrane permeability and affect biofilm formation in the bacterium. In planta experiments demonstrated a reduction in necrosis area in infected radishes treated with the extracts.
Review
Microbiology
Zhaoqing Yu, Wei Zhang, He Yang, Shan-Ho Chou, Michael Y. Galperin, Jin He
Summary: The authors review the diversity of sensory domains related to the bacterial CME regulation and specifically discuss those domains that are capable of sensing gaseous or light signals and the mechanisms they use for regulating cellular c-di-GMP levels.
FEMS MICROBIOLOGY REVIEWS
(2023)
Article
Plant Sciences
Maria Tortosa, Pablo Velasco, Victor Manuel Rodriguez, Maria Elena Cartea
Summary: Understanding plant's response mechanisms against pathogenesis is fundamental for resistant crop development. This study compared the proteomes of Brassica oleracea leaves infected with Xanthomonas campestris pv. campestris at different post-infection times. The results revealed that different cell processes, including primary metabolism, hormone signaling, and defense mechanisms, were involved in the plant's response to infection.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Filip Gazdik, Samuel Magnus, Steven J. Roberts, Rafal Baranski, Jana Cechova, Robert Pokluda, Ales Eichmeier, Dariusz Grzebelus, Miroslav Baranek
Summary: This study aimed to monitor the persistence of Xanthomonas campestris pv. campestris (Xcc) in field soil in central Europe, using different PCR assays. It was found that Xcc can persist in soil for up to two years, and the nested real-time PCR method showed higher sensitivity in detecting Xcc in soil compared to conventional PCR.
Article
Chemistry, Medicinal
Teng-Fei Xuan, Zi-Qiang Wang, Jun Liu, Hai-Tao Yu, Qian-Wen Lin, Wei-Min Chen, Jing Lin
Summary: The study proposed a new antibiofilm strategy involving the induction of dysfunctional G-quadruplexes by c-di-GMP to block the biofilm regulatory pathway. Compound 5h exhibited favorable c-di-GMP G-quadruplex-inducing and biofilm inhibitory activities, without any DNA intercalation effect. The performance of 5h in interfering with c-di-GMP-related biological functions was confirmed through reporter strain and transcriptome analysis.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Microbiology
Weiwei Yan, Yiming Wei, Susu Fan, Chao Yu, Fang Tian, Qi Wang, Fenghuan Yang, Huamin Chen
Summary: The study characterized the GGDEF domain protein GdpX6 in the rice blight pathogen Xoo, demonstrating its DGC activity and its role in controlling virulence, swimming and sliding motility, and biofilm formation.
Article
Agronomy
Jia-Bao Li, Lan-Tu Xiong, Yan-Rong Lu, Yu-Qing Zhang, Xiao-Li Xu, Hai-Hong Wang, Xin Deng, Xu-Hong Hu, Zi-Ning Cui
Summary: Cruciferous black rot, caused by Xcc infection, is a widespread disease globally. Excessive use of bactericide leads to bacterial resistance. This study discovered that compound SPF-9 inhibits virulence and pathogenicity of Xcc, showing potential as a novel T3SS inhibitor.
PEST MANAGEMENT SCIENCE
(2023)
Article
Microbiology
Anna Pecina, Meike Schwan, Vitan Blagotinsek, Tim Rick, Patrick Klueber, Tabea Leonhard, Gert Bange, Kai M. Thormann
Summary: This study identified a protein, MotL, which specifically regulates the activity of lateral flagellar motors in response to c-di-GMP levels. MotL, containing a PilZ domain binding c-di-GMP, slows down the rotation of lateral flagella at elevated c-di-GMP levels and may speed up the movement at low c-di-GMP concentrations. Interaction studies suggest an interaction of MotL with the C-ring of lateral flagellar motors, demonstrating differential c-di-GMP-dependent regulation of the two flagellar systems.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Qian Su, Xin-Xin Wang, Ming Leng, Yan-Hua Qi, Fu-Yuan Pang, Ji-Liang Tang, Guang-Tao Lu
Summary: This study investigated a previously uncharacterized nucleoid-associated protein, HU-like protein (Hlp), in Xcc, and found that Hlp affects virulence, extracellular polysaccharide production, and cell motility, with partially overlapping and complementary functions with the previously studied Fis-like protein (Flp). These findings contribute to a better understanding of the complex regulatory pathways involved in virulence in Xcc.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Immunology
Avinash Singh, Jerilyn R. Izac, Edward J. A. Schuler, Dhara T. Patel, Christopher Davies, Richard T. Marconi
Summary: This study presents the crystal structure of PIzA in complex with c-di-GMP, revealing its unique two-domain structure and the mechanism of c-di-GMP binding site formation. The information obtained will facilitate further research on the regulatory mechanisms of PlzA in ticks and mammals.
PATHOGENS AND DISEASE
(2021)
Article
Plant Sciences
Lu Lu, Sokrat G. Monakhos, Yong Pyo Lim, So Young Yi
Summary: The study identified significant differences in the expression of defense-related genes and antioxidant activity between black rot disease-resistant cabbage lines and susceptible lines, indicating a potential role for Xcc-induced reactive oxygen species in black rot disease development.
Article
Plant Sciences
Huiyong Xu, Hongfu Chen, Yuemao Shen, Liangcheng Du, Shan-Ho Chou, Hongxia Liu, Guoliang Qian, Fengquan Liu
Article
Biotechnology & Applied Microbiology
Yi-Chia Kuan, Yue-Bin Xu, Wen-Ching Wang, Ming-Te Yang
ENZYME AND MICROBIAL TECHNOLOGY
(2018)
Article
Multidisciplinary Sciences
Jai-Shin Liu, Yi-Chia Kuan, Yu Tsou, Tung-Yueh Lin, Wen-Hwei Hsu, Ming-Te Yang, Jong-Yih Lin, Wen-Ching Wang
SCIENTIFIC REPORTS
(2018)
Article
Genetics & Heredity
Chi-Ren Tsai, Hsiu-Fen Lee, Ching-Shiang Chi, Ming-Te Yang, Chia-Chi Hsu
Article
Multidisciplinary Sciences
Ming-Fen Huang, Shin-Jen Lin, Tzu-Ping Ko, Yi-Ting Liao, Kai-Cheng Hsu, Hao-Ching Wang
Article
Plant Sciences
Yi-Ting Liao, Shih-Shun Lin, Shin-Jen Lin, Wan-Ting Sun, Bing-Nan Shen, Han-Pin Cheng, Chan-Pin Lin, Tzu-Ping Ko, Yi-Fan Chen, Hao-Ching Wang
Article
Microbiology
Hung-Wei Chang, Cheng-Han Yang, Yu-Chun Luo, Bo-Gang Su, Huei-Yin Cheng, Shu-Yun Tung, Kathleen Joyce D. Carillo, Yi-Ting Liao, Der-Lii M. Tzou, Hao-Ching Wang, Wen Chang
Article
Microbiology
Michael Y. Galperin, Shan-Ho Chou
JOURNAL OF BACTERIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Jin He, Wen Yin, Michael Y. Galperin, Shan-Ho Chou
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Yi-Ting Liao, Shin-Jen Lin, Tzu-Ping Ko, Chang-Yi Liu, Kai-Cheng Hsu, Hao-Ching Wang
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Review
Microbiology
Wen Yin, Siyang Xu, Yiting Wang, Yuling Zhang, Shan-Ho Chou, Michael Y. Galperin, Jin He
Summary: Biofilms are complex microbial architectures that provide microorganisms with enhanced resistance against hostile environments. Harmful biofilms pose threats to food safety, plant and animal health, and medical fields, urging the development of effective control strategies. This review discusses various strategies to control biofilm formation, hoping to inspire innovative and effective approaches for managing harmful biofilms.
CRITICAL REVIEWS IN MICROBIOLOGY
(2021)
Review
Microbiology
Yuling Zhang, Siyang Xu, Yijun Yang, Shan-Ho Chou, Jin He
Summary: The emergence of antibiotic-resistant bacteria (ARB) in the human intestine is mainly due to the excessive use of antibiotics, with antibiotic-resistant genes (ARGs) primarily spreading through the gut. Various methods are needed to prevent the emergence of ARB and inhibit the spread of ARGs in the human intestine, and ultimately, an interdisciplinary 'One Health' approach may be able to overcome this challenge.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Microbiology
Michael Y. Galperin, Shan-Ho Chou
Summary: The HD-GYP domain is a specialized version of the HD phosphohydrolase domain that is involved in bacterial signal transduction and can hydrolyze the bacterial second messengers c-di-GMP and cGAMP. The study provides a refined sequence model for the HD-GYP domain and describes its roles in metal and/or substrate binding. The authors also calculate the numbers of HD-GYPs encoded in various genomes and list common domain combinations involving HD-GYP.
JOURNAL OF BACTERIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Zhou Li, He Yang, Yifei Wang, Shan-Ho Chou, Jin He
Summary: This article introduces the critical role of microbial cell factories in green biomanufacturing. It proposes the concept of the "spatial position effect" and uses 3D genomics technology to study the spatial structure characteristics of the chassis strain's 3D genome. It also suggests a method to design integration sites for synthetic gene circuits and achieve stable and efficient chassis strains for green biomanufacturing.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Eric H. -L. Chen, Chun-Hsiung Wang, Yi-Ting Liao, Feng-Yueh Chan, Yui Kanaoka, Takayuki Uchihashi, Koichi Kato, Longsheng Lai, Yi-Wei Chang, Meng-Chiao Ho, Rita P. -Y. Chen
Summary: The abuse of antibiotics has led to the emergence of multidrug-resistant microbial pathogens. Antimicrobial peptides (AMPs) have shown potential in combating these superbugs, but their mechanism of action is not fully understood. This study used high-resolution imaging techniques to directly observe the disruption of outer and inner membranes of Escherichia coli by a de novo-designed peptide, providing critical insights into the mechanisms of this class of antimicrobial agents.
NATURE COMMUNICATIONS
(2023)