Review
Biochemistry & Molecular Biology
Vinzent Schulz, Sven-A Freibert, Linda Boss, Ulrich Muehlenhoff, Oliver Stehling, Roland Lill
Summary: This review focuses on the structure, function and specificity of mitochondrial [2Fe-2S] FDXs and their important roles in various biological processes. Mitochondrial FDXs play crucial functions in steroid transformations, Fe/S protein biogenesis, and cofactor biosynthesis through electron transfer.
Article
Biochemistry & Molecular Biology
Danyun Zhang, Owen R. Dailey, Daniel J. Simon, Kamilah Roca-Datzer, Yasaman Jami-Alahmadi, Mikayla S. Hennen, James A. Wohlschlegel, Carla M. Koehler, Deepa Dabir
Summary: Yeast utilizes the MIA pathway to maintain redox-regulated import of cysteine-rich precursors, with Aim32 identified as a key protein interacting with sulfhydryl oxidase Erv1. Aim32 interacts with multiple proteins and plays a role in regulating the oxidation state of mitochondrial proteins.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
R. Sophia Weerth, Amy E. Medlock, Harry A. Dailey
Summary: The article explores the distribution and function of [2Fe-2S] clusters in ferrochelatases involved in heme synthesis, revealing that while these clusters are rare within the bacterial ferrochelatase family, they are prevalent in certain phyla and correlate with microbial aerobic metabolism. Analysis also suggests that Firmicute ferrochelatases are the most ancient and preceded the Alphaproteobacterial precursor to eukaryotic mitochondria, shedding light on the evolution of these clusters.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Ping Li, Amber L. Hendricks, Yong Wang, Rhiza Lyne E. Villones, Karin Lindkvist-Petersson, Gabriele Meloni, J. A. Cowan, Kaituo Wang, Pontus Gourdon
Summary: This study utilizes cryo-EM to reveal the structure of Atm1 transporter and proposes its molecular mechanism in the maturation of iron-sulfur proteins. These findings are of significant importance for understanding the transport mechanism of eukaryotic ABCB7-type proteins.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Christian Schneider, Sophie J. Gross, Serhiy Demeshko, Sebastien Bontemps, Franc Meyer, C. Gunnar Werncke
Summary: In this study, a unique diferrous [2Fe-2S](0) complex with only three-coordinate iron ions was synthesized by reducing a four-coordinate diferric [2Fe-2S](2+) complex and losing ligands. The obtained compounds were extensively analyzed for their properties using Fe-57 Mossbauer spectroscopy and magnetic susceptibility measurements. Additionally, facile cleavage of the [2Fe-2S] rhombus by CS2 was demonstrated.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Inorganic & Nuclear
Kady Oakley, Kevin Sterling, Jason Shearer, Eunsuk Kim
Summary: The study demonstrates how MitoNEET releases its [2Fe-2S] cluster under acidic conditions by controlling proton reactions to rearrange the cluster structure. Site-differentiated [2Fe-2S] clusters prepared experimentally undergo redox reactions with temperature changes.
INORGANIC CHEMISTRY
(2021)
Article
Biology
Henri-Baptiste Marjault, Ola Karmi, Ke Zuo, Dorit Michaeli, Yael Eisenberg-Domovich, Giulia Rossetti, Benoit de Chassey, Jacky Vonderscher, Ioav Cabantchik, Paolo Carloni, Ron Mittler, Oded Livnah, Eric Meldrum, Rachel Nechushtai
Summary: Marjault et al. report the crystal structures of a drug molecule bound to NEET proteins and its effects on diabetic mice. They found that this molecule can reduce hyperglycemia in diabetic mice without causing weight gain. This study suggests a new approach for managing diabetes by targeting NEET proteins.
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Alejandro Villalta, Batoul Srour, Audrey Lartigue, Martin Clemancey, Deborah Byrne, Florence Chaspoul, Antoine Loquet, Bruno Guigliarelli, Genevieve Blondin, Chantal Abergel, Benedicte Burlat
Summary: Scientists have discovered a protein in the giant virus mimivirus that is rich in the amino acids glycine and cysteine. This protein, called GciS, is one of the most abundant proteins in the viral particles and is likely essential for infection. It has unique binding properties with iron and inorganic sulfide.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Ola Karmi, Henri-Baptiste Marjault, Fang Bai, Susmita Roy, Yang-Sung Sohn, Merav Darash Yahana, Faruck Morcos, Konstantinos Ioannidis, Yaakov Nahmias, Patricia A. Jennings, Ron Mittler, Jose N. Onuchic, Rachel Nechushtai
Summary: The NEET protein family plays a crucial role in regulating mitochondrial labile iron and reactive oxygen species homeostasis. MiNT interacts with VDAC1 to transfer [2Fe-2S] clusters from mitochondria to the cytosol, impacting mitochondrial structure and function in breast cancer cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Michele Invernici, Giulia Selvolini, Jose Malanho Silva, Giovanna Marrazza, Simone Ciofi-Baffoni, Mario Piccioli
Summary: This study presents the effects of different iron-sulfide-glutathione ratios on the speciation of iron-sulfur cluster glutathione complexes. An excess of sulfide promotes the formation of a tetranuclear complex, while an excess of iron ions favors the formation of a dinuclear complex. These two complexes establish an interconversion equilibrium.
CHEMICAL COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Sven-A Freibert, Michal T. Boniecki, Claudia Stuempfig, Vinzent Schulz, Nils Krapoth, Dennis R. Winge, Ulrich Muehlenhoff, Oliver Stehling, Miroslaw Cygler, Roland Lill
Summary: The conserved N-terminal Tyr35 of ISCU2 plays a crucial mechanistic role in de novo mitochondrial [2Fe-2S] cluster synthesis, with ionic forces replacing Tyr-Tyr interactions during dimerization-induced [2Fe-2S] cluster formation. Hydrophobic interaction of two conserved N-terminal tyrosines induces ISCU2 dimerization and concomitant [2Fe-2S] cluster synthesis.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Sambuddha Sen, Amber L. Hendricks, James A. Cowan
Summary: Mitochondrial BOLA1 forms a [2Fe-2S] cluster-bridged heterodimeric complex with mitochondrial monothiol glutaredoxin GLRX5, with unclear function. This study explores the cluster exchange reactivity of the BOLA1-GLRX5 complex, demonstrating the ability to form the complex from certain donors but not others. The holo [2Fe-2S] BOLA1-GLRX5 complex is incapable of donating clusters to apo protein acceptors, providing evidence for a non-trafficking role. Additionally, the study compares the stability and cluster exchange reactivity of the holo-heterodimer and holo BOLA1 homodimer.
Article
Biology
Layla Drwesh, Benjamin Heim, Max Graf, Linda Kehr, Lea Hansen-Palmus, Mirita Franz-Wachtel, Boris Macek, Hubert Kalbacher, Johannes Buchner, Doron Rapaport
Summary: This study reconstituted the early cytosolic steps of signal-anchored (SA) protein biogenesis and identified molecular (co)chaperones, including Hsp70, Hsp90, and Hsp40 family co-chaperones, that interact with newly synthesized SA proteins. These interactions are mediated by the hydrophobic transmembrane segments of the SA proteins. The study also demonstrated the inhibitory effect of interfering with these interactions on SA protein biogenesis and successfully reconstituted the transfer of peptides from Hsp70 chaperone to the mitochondrial Tom70 receptor in vitro.
Article
Chemistry, Inorganic & Nuclear
Suman Dhara, Sanjib Panda, Goutam Kumar Lahiri
Summary: The facile reduction of 2,2'-dithiobisbenzothiazole through metal-to-ligand charge transfer or internal reducing equivalent is demonstrated, resulting in various binding modes of thiolates in a series of mononuclear and dinuclear ruthenium complexes. The electron transfer processes in the dinuclear complex resemble that of a [2Fe-2S] cluster.
DALTON TRANSACTIONS
(2021)
Article
Chemistry, Inorganic & Nuclear
Maddalena Corsini, Piero Zanello
Summary: Although archaea do not possess certain multiprotein machinery found in bacteria and eukarya, some of them are capable of encoding Fe-S proteins. This study focuses on the enzymatic reactions triggered by structurally characterized members of the archaeal family, emphasizing their biological functions and, when available, their electrochemical behavior.
Article
Biochemistry & Molecular Biology
Dominique T. Capraro, David J. Burban, Patricia A. Jennings
JOURNAL OF MOLECULAR BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Renee Otten, Ricardo A. P. Padua, H. Adrian Bunzel, Vy Nguyen, Warintra Pitsawong, MacKenzie Patterson, Shuo Sui, Sarah L. Perry, Aina E. Cohen, Donald Hilvert, Dorothee Kern
Article
Mathematics, Interdisciplinary Applications
Ezgi Ayturk, Heining Cham, Patricia A. Jennings, Joshua L. Brown
Summary: The study found that LMS-cat is excellent at estimating structural parameters but may produce highly biased measurement parameters. The performance of LMS-cat varies under different conditions, as revealed by the simulation study.
STRUCTURAL EQUATION MODELING-A MULTIDISCIPLINARY JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Shuo Sui, Anne Mulichak, Raviraj Kulathila, Joshua McGee, Danny Filiatreault, Sarthak Saha, Aina Cohen, Jinhu Song, Holly Hung, Jonathan Selway, Christina Kirby, Om K. Shrestha, Wilhelm Weihofen, Michelle Fodor, Mei Xu, Rajiv Chopra, Sarah L. Perry
Summary: This novel approach utilizes capillary-based microfluidic devices and X-ray crystallography to accelerate the process of small-molecule-compound screening. The devices are designed for efficient sample manipulation and crystal handling, opening up new possibilities for research in this field.
JOURNAL OF APPLIED CRYSTALLOGRAPHY
(2021)
Article
Physiology
Cynthia S. Indralingam, Alma K. Gutierrez-Gonzalez, Scott C. Johns, Tzuhan Tsui, Daniel T. Cannon, Mark M. Fuster, Timothy D. Bigby, Patricia A. Jennings, Ellen C. Breen
Summary: Pulmonary arterial hypertension (PAH) is a severe disease characterized by pulmonary artery remodeling, elevated right ventricular pressure, and cardiac failure. Pulmonary endothelial cells play a crucial role in PAH by sensing danger or damage and triggering cell proliferation through alarmin cytokines. This study found that IL-33/ST2 signaling is essential for the proliferative response of pulmonary artery endothelial cells in PAH.
PHYSIOLOGICAL REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Izumi Ishigami, Silvia Russi, Aina Cohen, Syun-Ru Yeh, Denis L. Rousseau
Summary: This study investigated the relationship between the structural integrity of bovine CcO in the fully oxidized state and synchrotron radiation using microspectroscopy-coupled X-ray crystallography. The results showed that X-ray exposure caused the conversion of the oxidized state (O) to a hybrid state (O*) in which all four metal centers were reduced, but the protein matrix remained in the O conformation and the ligands in the BNC were intact. Annealing the O* crystal induced the relaxation of the structure to a new state (R*) where the protein matrix converted to the fully reduced R conformation, except for partial retention of the O conformation in helix X.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Galen J. Correy, Daniel W. Kneller, Gwyndalyn Phillips, Swati Pant, Silvia Russi, Aina E. Cohen, George Meigs, James M. Holton, Stefan Gahbauer, Michael C. Thompson, Alan Ashworth, Leighton Coates, Andrey Kovalevsky, Flora Meilleur, James S. Fraser
Summary: This study determined the crystal structures of the nonstructural protein 3 (NSP3) macrodomain of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), revealing the reorganization of water networks upon binding of ADP-ribose and other ligands. The catalytic mechanism of coronavirus macrodomains was found to be distinct from that of human macrodomains, providing insights for the development of inhibitors.
Article
Psychology, Educational
Polina P. Mischenko, Pamela Nicholas-Hoff, Deborah L. Schussler, Jessica Iwu, Patricia A. Jennings
Summary: This study provides an overarching analysis on the barriers and facilitators that implementers experienced in delivering a mindfulness-based social-emotional learning program. It emphasizes the importance of relational trust between implementers and actors within various ecological levels. The study applies a framework of relational trust to understand the relationships that influence the effectiveness of program delivery.
PSYCHOLOGY IN THE SCHOOLS
(2022)
Editorial Material
Psychology, Clinical
Patricia A. Jennings
Summary: There is a growing interest in studying the impacts of mindfulness-based programs in PreK-12 school settings, but the research lacks rigor and the results have been mixed. Baelen et al. present a conceptual framework for implementing school-based mindfulness programs, which will contribute to building a more solid evidence base. It is important to simultaneously focus on implementation practice to bridge the gap between knowledge and practice.
Article
Education & Educational Research
Holly E. Brophy-Herb, Ahnalee Brincks, Jody L. Cook, Ann Stacks, Claire D. Vallotton, Cynthia Frosch, Russell Carson, Rebecca Wheeler, Haiden A. Perkins, Patricia A. Jennings
Summary: This study examined the perceptions of stress and exhaustion among infant/toddler teachers. The findings revealed that workload and personal life stressors were associated with stress and exhaustion. Teachers used fewer than two coping strategies per day, and reframing was the only strategy negatively associated with stress and emotional exhaustion. The study highlights the need for professional development supports and workload reduction for infant/toddler teachers.
EARLY EDUCATION AND DEVELOPMENT
(2023)
Article
Education & Educational Research
Holly E. Brophy-Herb, Ann M. Stacks, Cynthia Frosch, Ahnalee M. Brincks, Jody L. Cook, Claire D. Vallotton, Haiden A. Perkins, Loria E. Kim, Russell Carson, Maria Muzik, Katherine Rosenblum, Patricia A. Jennings
Summary: The study focused on a relationship-based professional development intervention for infant and toddler teachers to enhance their use of mindfulness-based strategies for coping. While the intervention did not have an impact on stress and exhaustion trajectories, teachers in the intervention group reported increased use of mindfulness-based coping strategies over time.
EARLY CHILDHOOD EDUCATION JOURNAL
(2022)
Review
Psychology, Clinical
Patricia A. Jennings, Helen H. Min
Summary: Teachers' social and emotional competence and well-being are crucial for creating and maintaining supportive learning environments. However, teachers often lack the necessary support to provide trauma-sensitive care to students who have experienced adversity. This review explores the concept of empathy-based stress and proposes that shifting towards compassionate responding can help teachers effectively meet students' needs while protecting their own well-being.
Article
Biochemical Research Methods
Zhongrui Liu, Kevin K. Gu, Megan L. Shelby, Deepshika Gilbile, Artem Y. Lyubimov, Silvia Russi, Aina E. Cohen, Sankar Raju Narayanasamy, Sabine Botha, Christopher Kupitz, Raymond G. Sierra, Fredric Poitevin, Antonio Gilardi, Stella Lisova, Matthew A. Coleman, Matthias Frank, Tonya L. Kuhl
Summary: Over the past two decades, serial X-ray crystallography has made significant progress in determining the structures of proteins. This study presents an improved design of a polymer microfluidic chip that facilitates the delivery of fragile and small crystals to X-ray beams. The chip is customizable for different types of crystals and offers a user-friendly, cost-effective, and robust sample-delivery platform. Experimental results demonstrate the effectiveness of the chip design in obtaining high-resolution data sets for various proteins. The improved chip design provides a powerful sample-delivery tool for structural biologists, especially when working with limited sample volumes and fragile crystals.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2023)
Article
Psychology, Clinical
Sebrina L. Doyle L. Fosco, Deborah L. Schussler, Patricia A. Jennings
Summary: This research investigated how school and district leaders perceive and respond to an adapted version of the CARE program. The study found that acceptability of the program was inconsistent among educational leaders, with those with prior mindfulness experience benefiting the most. The format of the program also influenced acceptability, with many leaders finding it too long. However, all leaders reported using CARE practices and saw improvements in intrapersonal and interpersonal aspects.
Article
Biochemical Research Methods
Sarthak Saha, Can oezden, Alfred Samkutty, Silvia Russi, Aina Cohen, Margaret M. Stratton, Sarah L. Perry
Summary: Proteins are essential for various biological functions and their structure determines their function. X-ray crystallography is used to determine protein structure, but protein crystallization is required. We have developed a microfluidic device that can handle and measure fluids for protein crystallization without the need for additional connections. The use of UV-curable polymers allows for in situ X-ray crystallography, simplifying the analysis process. We demonstrate the device's utility using lysozyme and CaMKII beta as examples.