Article
Biochemistry & Molecular Biology
Han Gao, Jia-Qi Li, Peng-Wei Kang, Jia-Zhu Chigan, Huan Wang, Lu Liu, Yin-Sui Xu, Le Zhai, Ke-Wu Yang
Summary: This study constructed and characterized an effective M beta L inhibitor, which showed preferential inhibition against NDM-1, potential antibacterial activities, and synergistic effects with meropenem. The research provided a new framework for the development of NDM-1 inhibitors.
BIOORGANIC CHEMISTRY
(2021)
Article
Microbiology
Amrita Bhattacharjee, Kirsty Sands, Shravani Mitra, Ritojeet Basu, Bijan Saha, Olivier Clermont, Shanta Dutta, Sulagna Basu
Summary: This study analyzed 80 isolates of Escherichia coli collected from neonates with sepsis between 2009 and 2019. The majority of the isolates were multidrug-resistant and 44% were carbapenem-resistant due to the presence of the bla(NDM) gene. Different phylogroups and clonal complexes were identified, with ST167 and ST131 being the most common epidemic clones.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Le Zhai, Yue Jiang, Yang Shi, Miao Lv, Ya-Li Pu, Hua-Lei Cheng, Jia-Yu Zhu, Ke-Wu Yang
Summary: The irregular use of antibiotics has led to the development of antibiotic-resistant bacteria, specifically metallo-beta-lactamases. This study identified eight compounds that have strong inhibitory effects on NDM-1, with one compound being the most effective inhibitor. The presence of a hydroxyl group in the compounds was found to improve their inhibitory activity. Additionally, these compounds demonstrated antibacterial effects on NDM-1-producing bacteria and showed low cytotoxicity in cell experiments.
BIOORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jia-Qi Li, Le-Yun Sun, Zhihui Jiang, Cheng Chen, Han Gao, Jia-Zhu Chigan, Huan-Huan Ding, Ke-Wu Yang
Summary: A new scaffold was constructed for inhibiting NDM-1, showing promising results in combating bacterial infections and providing important insights for the development of anti-NDM-1 drugs.
BIOORGANIC CHEMISTRY
(2021)
Review
Microbiology
Xiuyun Li, Jing Zhao, Bin Zhang, Xuexia Duan, Jin Jiao, Weiwei Wu, Yuxia Zhou, Hefeng Wang
Summary: β-lactams are powerful tools for combating bacterial infections, but their effectiveness is threatened by drug resistance. Metallo-β-lactamases are now increasingly threatening and pose a global challenge to public health. This review provides an overview of potential candidates, including new antibacterial drugs, non-antibacterial drugs, antimicrobial peptides, natural products, and zinc chelators, to combat metallo-β-lactamase producers.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Jacqueline Findlay, Laurent Poirel, Julie Kessler, Andreas Kronenberg, Patrice Nordmann
Summary: The study in Switzerland found that NDM-positive CPE, primarily consisting of Klebsiella pneumoniae and Escherichia coli, were prevalent, with NDM-1 and NDM-5 variants being the most common. Some isolates also co-produced a second carbapenemase and a 16S rRNA methylase. Successful plasmids and global lineages were identified as major factors contributing to the increasing prevalence of NDMs in Switzerland.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Immunology
Eduardo Rodriguez-Noriega, Elvira Garza-Gonzalez, Paola Bocanegra-Ibarias, Beatriz Alejandra Paz-Velarde, Sergio Esparza-Ahumada, Esteban Gonzalez-Diaz, Hector R. Perez-Gomez, Rodrigo Escobedo-Sanchez, Gerardo Leon-Garnica, Rayo Morfin-Otero
Summary: This study identified the previous use of antibiotics, the use of a urinary catheter, the use of a central venous catheter, the use of mechanical ventilation, and ICU stay as predictors and independent risk factors for infection with NDM-1 K. pneumoniae.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Bejan Mahmud, Meghan A. Wallace, Kimberly A. Reske, Kelly Alvarado, Carol E. Muenks, David A. Rasmussen, Carey-Ann D. Burnham, Cristina Lanzas, Erik R. Dubberke, Gautam Dantas
Summary: ESBL-producing Escherichia coli is becoming increasingly prevalent, driven by the spread of ESBL-encoding plasmids. This study provides an in-depth genomic investigation of clinical ESBL-like E. coli isolates, revealing a comprehensive map of plasmid sharing across different boundaries. Through sequence-based network analysis, specific plasmid lineages responsible for disseminating major ESBLs have been identified. This work highlights the importance of plasmid-mediated spread in the dissemination of ESBLs and provides valuable insight into the factors underlying the increased prevalence of these genes in clinical settings.
Article
Multidisciplinary Sciences
Vikram Khipple Mulligan, Sean Workman, Tianjun Sun, Stephen Rettie, Xinting Li, Liam J. Worrall, Timothy W. Craven, Dustin T. King, Parisa Hosseinzadeh, Andrew M. Watkins, P. Douglas Renfrew, Sharon Guffy, Jason W. Labonte, Rocco Moretti, Richard Bonneau, Natalie C. J. Strynadka, David Baker
Summary: This study presents a structure-guided computational method for designing peptide macrocycles using a mixture of L- and D-amino acids to inhibit therapeutic targets. The experimental results show that this method can effectively predict peptide binding propensity and inhibitory activity, as demonstrated in the interaction with NDM-1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Pharmacology & Pharmacy
Yan Guo, Hongtao Liu, Mengge Yang, Rui Ding, Yawen Gao, Xiaodi Niu, Xuming Deng, Jianfeng Wang, Haihua Feng, Jiazhang Qiu
Summary: The study identified dexrazoxane, embelin, candesartan cilexetil, and nordihydroguaiaretic acid as promising carbapenem adjuvants against metallo-β-lactamase-producing carbapenem-resistant bacterial pathogens. These drugs inhibit the activity of metallo-β-lactamases and show in vitro synergistic bactericidal effect with meropenem.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Anka Lucic, Philip Hinchliffe, Tika R. Malla, Catherine L. Tooke, Jurgen Brem, Karina Calvopina, Christopher T. Lohans, Patrick Rabe, Michael A. McDonough, Timothy Armistead, Allen M. Orville, James Spencer, Christopher J. Schofield
Summary: Penems have shown potential as antibacterials and beta-lactamase inhibitors, but their clinical use is limited compared to carbapenems. Faropenem, with a C-2 tetrahydrofuran ring, is resistant to some beta-lactamases. Studies on reactions of faropenem with beta-lactamases demonstrate different outcomes, suggesting further research is needed for optimizing the interactions between penems and beta-lactamases.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Alessandra Piccirilli, Emanuele Criscuolo, Fabrizia Brisdelli, Paola Sandra Mercuri, Sabrina Cherubini, Maria Laura De Sciscio, Mauro Maccarrone, Moreno Galleni, Gianfranco Amicosante, Mariagrazia Perilli
Summary: Study on NDM-1 mutants revealed that mutations in leucine residues can modify the hydrolytic profile of the enzyme against certain beta-lactams, with L218T and L221T showing significant reduction in hydrolysis efficiency. The stability of the mutants was explored through thermofluor assay, showing NDM-1 and L218T to be the most stable enzymes. Molecular dynamic studies justified differences in kinetic behavior observed among the mutants.
Article
Biochemistry & Molecular Biology
Lalitha Cheepurupalli, Aathithya Diaz, Adithya Conjeevaram Gopal, Sudarshan Singh Rathore, Vigneshwar Ramakrishnan, Jayapradha Ramakrishnan
Summary: The increase in drug resistance poses a major threat to public health, with carbapenem-resistant bacteria being a significant concern leading to higher morbidity and mortality rates. Inhibiting the activity of New Delhi metallo-beta-lactamase-1 is seen as a potential way to treat multidrug-resistant pathogens. The study identified potential inhibitors for New Delhi metallo-beta-lactamase-1, providing insights into novel treatment strategies for combating drug resistance.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Microbiology
Dennis Nurjadi, Kaan Kocer, Quan Chanthalangsy, Sabrina Klein, Klaus Heeg, Sebastien Boutin
Summary: This study found that the emergence of resistance to Cefiderocol in metallo-beta-lactamase-producing bacteria is facilitated by mutations in the CirA siderophore receptor, which are induced by the New Delhi metallo-beta-lactamase. However, inhibiting the metallo-beta-lactamase activity using dipicolinic acid can successfully prevent the development of Cefiderocol-resistant mutants. Therefore, caution should be taken when using Cefiderocol to treat infections caused by metallo-beta-lactamase-producing bacteria.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Anka Lucic, Tika R. Malla, Karina Calvopina, Catherine L. Tooke, Jurgen Brem, Michael A. McDonough, James Spencer, Christopher J. Schofield
Summary: This study investigated the reaction of the carbapenem biapenem with different subclasses of beta-lactamases. The results showed the formation of enamine and various types of imine products. The findings support the idea that prolonging the lifetime of beta-lactamase carbapenem complexes by optimizing the tautomerization process could lead to improved carbapenems.
Article
Infectious Diseases
Shrikala Baliga, Christina Murphy, Leesha Sharon, Suchitra Shenoy, Dhanashree Biranthabail, Helena Weltman, Steve Miller, Ranjan Ramasamy, Jyotsna Shah
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2018)
Article
Public, Environmental & Occupational Health
Pradeepa H. Dakappa, Sathish B. Rao, B. Ganaraja, Gopalkrishna K. Bhat, Chakrapani Mahabala
Article
Infectious Diseases
Dhiraj Krishna, Biranthabail Dhanashree
Summary: The study focused on the biofilm formation, antibiotic susceptibility, and virulence genes of Salmonella enterica serovars Typhi and Paratyphi, which are causative agents of enteric fever. The results demonstrated variable biofilm formation and antibiotic resistance patterns among the isolates. Presence of invA and spvC genes indicated their ability of invasiveness and intracellular survival.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Agriculture, Dairy & Animal Science
Khyati Bhardwaj, Suchitra M. Shenoy, Shrikala Baliga, B. Unnikrishnan, B. Shantharam Baliga, Vasanth Kumar Shetty
Summary: The study conducted in poultry farms of coastal Southern Karnataka, India, found high levels of antibiotic resistance in Escherichia coli and Klebsiella pneumoniae isolates, with a focus on resistance to fluoroquinolones and tetracycline. The most common resistance genes detected were qnrB, qnrS, and qnrA, with most isolates showing high MAR indices reflecting widespread antibiotic use.
Article
Biology
Anoop Jaiswal, Monica Singh, Arindam Chakraborty
Summary: This study compared the performance of the Truenat assay with four RT-PCR assay kits and found that Truenat RT-PCR is a more reliable technique for the detection of SARC-CoV-2.
ADVANCES IN HUMAN BIOLOGY
(2022)
Article
Infectious Diseases
K. Archana Bhat, Deepak Madi, Sevitha Bhat, Therese Mary, Shalini Shenoy Mulki, Himani Kotian
Summary: Secondary bacterial infections with multi-drug-resistant bacteria in COVID-19 patients were studied to evaluate their impact on the outcome. The proportion and spectrum of these infections, as well as the co-existing comorbidities, treatment, and outcome in the patients were determined.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
V. Vandhana, K. Vishwas Saralaya, Sevitha Bhat, Shalini Shenoy Mulki, Archana. K. Bhat
Summary: This study identified the emerging variant of Klebsiella pneumoniae, hypervirulent K. pneumoniae (Hv-Kp), as causing infections with higher mortality rates and increased multidrug resistance in immunocompetent individuals. Early identification of Hv-Kp strains and determination of their antimicrobial susceptibility patterns are crucial for providing appropriate treatment and preventing potential outbreaks.
INTERNATIONAL JOURNAL OF MICROBIOLOGY
(2022)
Article
Immunology
Archana Bhat, Suchetha S. Rao, Sevitha Bhat, Katara Vidyalakshmi, Biranthabail Dhanashree
Summary: This study aims to detect common paediatric viral and bacterial diarrhoea pathogens using microscopy, stool culture, and multiplex polymerase chain reaction (mPCR). The results showed that Shigella spp. and rotavirus are the main causative agents of childhood diarrhoea in the region. The rate of detection of bacterial aetiology by culture was low, while mPCR proved to be a better choice for early detection of pathogens, ensuring timely diagnosis, treatment, and a reduction in mortality.
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
M. Sreejisha, Shalini Shenoy Mulki, Suchitra Shenoy, Biranthabail Dhanashree, M. Chakrapani, Gopalakrishna Bhat
Summary: This study investigated infections caused by methicillin resistant Staphylococcus aureus (MRSA) in diabetic and non-diabetic patients in four tertiary care hospitals in Coastal Karnataka, South India, with a focus on heterogeneous vancomycin intermediate S. aureus (hVISA). The results showed that the rate of hVISA among all MRSA isolates was 6.4%, and the risk of hVISA infection was three times higher in diabetic patients.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Biotechnology & Applied Microbiology
Monica Singh, Arindam Chakraborty, Anuj Kumar Tyagi
Summary: This study examines the frequency of false seropositivity of Salmonella specific antibody in Dengue and COVID-19 patients. The results suggest that caution should be exercised when reporting false positive serological tests, and repeated testing should be conducted until the etiological agents are confirmed to minimize misdiagnosis and prevent morbidity and mortality.
JOURNAL OF PURE AND APPLIED MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Pedduru Mallikarjuna, Biranthabail Dhanashree
Summary: This study assessed the colistin minimum inhibitory concentration (MIC), biofilm formation, presence of mod A and psl A genes, and types of motilities in multidrug-resistant (MDR) and multidrug-susceptible (MDS) P. aeruginosa. The findings revealed a significant association among strong biofilm formation, modA and pslA genes, and drug resistance in P. aeruginosa isolated from clinical samples.
JOURNAL OF TAIBAH UNIVERSITY MEDICAL SCIENCES
(2023)
Article
Public, Environmental & Occupational Health
Khyati Bhardwaj, Suchitra M. Shenoy, Shrikala Baliga, B. Unnikrishnan, B. Shantharam Baliga
Summary: The study revealed that most respondents have a fairly appropriate attitude towards accessing antibiotics, but there are misconceptions regarding antibiotic use and effects. Knowledge regarding antibiotic resistance and its causes is limited, highlighting the need for creating awareness among the public on the prudent use of antibiotics in the future.
CLINICAL EPIDEMIOLOGY AND GLOBAL HEALTH
(2021)
Article
Computer Science, Interdisciplinary Applications
Sridevi Saralaya, Vishwas Saralaya, Rio D'Souza
SERVICE ORIENTED COMPUTING AND APPLICATIONS
(2020)
Article
Microbiology
Munmun B. Marak, Biranthabail Dhanashree
INTERNATIONAL JOURNAL OF MICROBIOLOGY
(2018)