期刊
ACTA BIOMATERIALIA
卷 10, 期 5, 页码 2014-2023出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2014.01.022
关键词
Silk fibroin scaffolds; Freeze-drying; Neovascularization; Heparin; Vascular graft
资金
- National Natural Science Foundation of China (NSFC) [51173084, 81171478, 31100699]
- National Program on Key Basic Research Project [2011CB606202]
- Tianjin Research Program of Application Foundation and Advanced Technology [11 JCYBJC 10300]
Silk fibroin (SF) scaffolds have been designed and fabricated for multiple organ engineering owing to SF's remarkable mechanical property, excellent biocompatibility and biodegradability, as well as its low immunogenicity. In this study, an easy-to-adopt and mild approach based on a modified freeze-drying method was developed to fabricate a highly interconnected porous SF scaffold. The physical properties of the SF scaffold, including pore morphology, pore size, porosity and compressive modulus, could be adjusted by the amount of ethanol added, the freezing temperature and the concentration of SF. Fourier transform infrared spectroscopy illustrated that treatment of the lyophilized scaffolds with 90% methanol led to a structure transition of SF from silk I (random coil) to silk II (beta-sheet), which stabilized the SF scaffolds in water. We also incorporated heparin during fabrication to obtain a heparin-loaded scaffold which possessed excellent anticoagulant property. The heparin that was incorporated into the SF scaffolds could be released in a sustain manner for approximately 7 days, inhibiting the proliferation of human smooth muscle cells within the scaffold in vitro while promoting neovascularization in vivo. We therefore propose that the SF porous scaffold fabricated here may be an attractive candidate for use as a potential vascular graft for implantation based on its high porosity, excellent blood compatibility and mild fabrication process. (C) 2014 Acts Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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