Article
Chemistry, Multidisciplinary
Mi-Ran Ki, Ki Sung Park, Mohamed A. A. Abdelhamid, Seung Pil Pack
Summary: Efforts to mimic nature have led to the development of a novel silica polymerization protein, AqCtLSN, which can produce silica in an environmentally friendly manner. AqCtLSN demonstrated silica-condensing activity and self-assembly properties, allowing for the fabrication of a silica-hybrid material. This material exhibited enhanced cell proliferation, alkaline phosphatase activity, and calcium mineral precipitation, making it suitable for biomedical applications, particularly bone regeneration.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Environmental Sciences
Marina Pozzolini, Ivan Miksik, Stefano Ghignone, Caterina Oliveri, Eleonora Tassara, Marco Giovine
Summary: This study describes the protein content of sponge spicules in Petosia ficiformis using a combination of transcriptomic and proteomic analysis. The results reveal a diverse group of proteins, including enzymes that may be related to lysosomes, suggesting a possible evolutionary origin of the biosilica machinery in sponges.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Kasun Godigamuwa, Kazunori Nakashima, Sota Tsujitani, Satoru Kawasaki
Summary: The fusion of silicatein with other proteins allows for its catalytic activity under mild conditions, making it suitable for immobilization on materials like chitin. This technique opens up possibilities for the development of novel biocatalytic systems and biosensors in various fields.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Chemistry, Applied
Yanhui Kong, Wenjing Zhang, Tian He, Xue Yang, Wanghua Bi, Jiwei Li, Wenzhe Yang, Weichao Chen
Summary: In this study, a bilayered asymmetric nanofibrous membrane was prepared, with a hydrophilic chitosan/chitosan oligosaccharide (COS) nanofibrous membrane as the bottom layer and a hydrophobic polycaprolactone (PCL) nanofibrous membrane as the top layer. Results showed that incorporating COS improved the wettability of the CS membrane and increased the antibacterial effects against Escherichia coli and Staphylococcus aureus. The PCL layer prevented water and bacteria adhesion. This wettable membrane has the potential to serve as a new antibacterial dressing for wound healing.
CARBOHYDRATE POLYMERS
(2023)
Article
Biochemistry & Molecular Biology
Heinz C. Schroeder, Xiaohong Wang, Meik Neufurth, Shunfeng Wang, Rongwei Tan, Werner E. G. Mueller
Summary: Two biocatalytically produced inorganic biomaterials, bio-silica and bio-polyphosphate (bio-polyP), have shown great potential in regenerative medicine and other medical applications. Bio-silica is synthesized by a group of enzymes called silicateins, which form amorphous hydrated silica from monomeric precursors. Bio-polyP has morphogenetic activity and can induce cell differentiation, as well as provide metabolic energy through enzymatic cleavage of its high-energy phosphoanhydride bonds. Silicatein and alkaline phosphatase are key enzymes involved in the metabolism and biological activity of these materials.
Article
Chemistry, Medicinal
Stefania Lama, Amalia Luce, Giuseppe Bitti, Pilar Chacon-Millan, Annalisa Itro, Pasquale Ferranti, Giovanni D'Auria, Marcella Cammarota, Giovanni Francesco Nicoletti, Giuseppe Andrea Ferraro, Chiara Schiraldi, Michele Caraglia, Evzen Amler, Paola Stiuso
Summary: This study investigated the potential application of polycaprolactone/polydatin (PCL/PD) nanofiber scaffold in bone tissue engineering. The results showed that osteosarcoma cells and mesenchymal stem cells cultured on PD-PCL nanofiber scaffold exhibited enhanced cell adhesion and osteogenic differentiation.
Article
Biochemistry & Molecular Biology
Xiaojie Sun, Jing Li, Kai Shao, Chang Su, Shichao Bi, Yuzhi Mu, Kaichao Zhang, Zheng Cao, Xiaoye Wang, Xiguang Chen, Chao Feng
Summary: The AC-DB sponge demonstrated rapid hemostatic ability and good biocompatibility, activating intrinsic coagulation pathway to accelerate clotting process, with shortest clotting time and minimal blood loss in vivo assessments.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Zheng Cao, Chang Su, Xiaojie Sun, Kai Shao, Xiaoye Wang, Yuzhi Mu, Xiguang Chen, Chao Feng
Summary: The temperature-sensitive hydrogel composed of diatom biosilica and hydroxybutyl chitosan shows significantly enhanced mechanical strength and favorable blood compatibility and biocompatibility. By adjusting the ratio of diatom biosilica and hydroxybutyl chitosan, the properties of the hydrogel can be flexibly controlled.
CARBOHYDRATE POLYMERS
(2022)
Article
Polymer Science
Hasan Subhi, Adam Husein, Dasmawati Mohamad, Nik Rozainah Nik Abdul Ghani, Asma-Abdullah Nurul
Summary: APC-CT is a promising material for endodontic use, as it enhances cell attachment and dentinogenic/osteogenic differentiation, promoting mineralization activity in stem cells.
Article
Biochemistry & Molecular Biology
Wentao Shi, Xuan Zhang, Lu Bian, Yao Dai, Zhe Wang, Yanjun Zhou, Shuang Yu, Zhijian Zhang, Peng Zhao, Hong Tang, Qing Wang, Xiaojie Lu
Summary: In this study, the researchers evaluated the osteogenic differentiation of ectomesenchymal stem cells (EMSCs) using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL@AL) in vitro. The results showed that the CS/PCL@AL scaffold enhanced osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10, and laminin expression, as well as activating the yes-associated protein (YAP) signaling pathway. In vivo experiments in rats with calvarial defects also demonstrated that the CS/PCL@AL scaffold promoted new and mature bone formation. This study highlights the potential of CS/PCL@AL scaffolds as an efficient delivery system for bone defect repair.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Physical
Azin Rashidy Ahmady, Atefeh Solouk, Saeed Saber-Samandari, Somaye Akbari, Hadi Ghanbari, Bogumil E. Brycki
Summary: Nano-in-nano delivery technique is explored to address the limitations of most nanostructures used for drug delivery. This study investigated the release behavior of a novel nanoparticles-in-nanofibers delivery system composed of capsaicin-loaded alginate nanoparticles embedded in polycaprolactone-chitosan nanofiber mats. The results showed that embedding capsaicin-loaded alginate nanoparticles in polycaprolactone-chitosan nanofibers prolonged capsaicin release and effectively inhibited the proliferation of MCF-7 human breast cells.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Physical
Hanyang Li, Zainen Qin, Yiqiang Ouyang, Bo Zheng, Hong Wei, Jun Ou, Chong Shen
Summary: A hydroxyapatite/chitosan-metformin (HA/CS-MF) composite coating was prepared on AZ31 magnesium alloy by hydrothermal treatment to improve the corrosion resistance and osteogenic activity. The coating exhibited excellent biocompatibility, anti-inflammatory activity, and upregulated bone-related gene expression.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Article
Biophysics
Hussein E. Rozan, Guangsheng Wu, Zhongzheng Zhou, Qinfeng Li, Mohamed Sharaf, Xiguang Chen
Summary: In this study, a hydroxybutyl chitosan/diatom biosilica/doxycycline composite hydrogel was developed, which exhibited significant antibacterial activity and biocompatibility. It could promote wound healing and show potential for clinical applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Iman Shirzaei Sani, Mostafa Rezaei, Ali Baradar Khoshfetrat, Donya Razzaghi
Summary: Chitosan and poly (epsilon-caprolactone) were combined to fabricate bone tissue engineering scaffolds with enhanced bioactivity and mechanical properties. The incorporation of chitosan-g-poly (epsilon-caprolactone) significantly decreased fiber diameter and hydroxyapatite nanoparticles enhanced mineralization and degradation of the scaffold. In vitro cytocompatibility assays showed high cell viability and proliferation, indicating the potential use of the microfabricated nanocomposite scaffolds in bone tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Nanoscience & Nanotechnology
Yiwen Dong, Litao Yao, Lei Cai, Mi Jin, Tymour Forouzanfar, Lianjun Wu, Jinsong Liu, Gang Wu
Summary: In this study, an antibacterial and pro-osteogenic coaxially electrospun nanofiber guided bone regeneration (GBR) membrane was developed to meet the complex requirements of the GBR process. The membrane exhibited excellent antibacterial properties and promoted bone regeneration, making it a promising approach for patients with large-volume bone defects caused by periodontitis or peri-implantitis.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Samy Yousef, Justas Sereika, Andrius Tonkonogovas, Tawheed Hashem, Alaa Mohamed
Summary: This research aims to study the influence of high temperatures and pressure on the selectivity performance of Polyethersulfone (PES) membranes for CO2/N2, CO2/H2, and CO2/CH4, and their potential applications in biogas upgrading systems. The results showed that low pressure and high temperature can enhance the selectivity performance and improve gas permeability. PES membranes are considered a promising technology for biogas upgrading with high selectivity, especially at high separation temperatures and low pressure.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Biochemistry & Molecular Biology
Alaa Mohamed, Elvia P. Valadez Sanchez, Evgenia Bogdanova, Britta Bergfeldt, Ammar Mahmood, Roman V. Ostvald, Tawheed Hashem
Summary: UiO-66-NH2 CNM exhibited high fluoride removal efficiency with a maximum adsorption capacity of 95 mg/L, which showed better fitting with the pseudo-second-order model. As the initial fluoride concentration increased from 20 to 100 mg/L after 60 min, fluoride adsorption decreased by 57% for UiO-66 CNM and 30% for UiO-66-NH2 CNM.
Article
Biochemistry & Molecular Biology
Tawheed Hashem, Elvia P. Valadez Sanchez, Evgenia Bogdanova, Anna Ugodchikova, Alaa Mohamed, Matthias Schwotzer, Mohamed H. Alkordi, Christof Woell
Summary: Metal-organic frameworks (MOFs) are intensively explored for novel applications and as alternatives to existing materials in thin film nanotechnologies. The stability of SURMOFs in harsh aqueous environments varies, with UiO-66-NH2 SURMOFs showing stability over a large window of pH values.
Article
Engineering, Mechanical
Alaa Mohamed, Samy Yousef, Tawheed Hashem, Mohammed Ali Abdelnaby
Summary: The mechanical properties of PES membranes and their nanocomposites were studied, showing a significant reduction in mechanical properties due to the addition of nanofillers altering the pore topology. The constitutive model effectively derived the uniaxial stress-strain curves of PES membranes and their nanocomposites with high predictability.
INTERNATIONAL JOURNAL OF MECHANICAL SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Raphael Greifenstein, Tim Ballweg, Tawheed Hashem, Eric Gottwald, David Achauer, Frank Kirschhoefer, Michael Nusser, Gerald Brenner-Weiss, Elaheh Sedghamiz, Wolfgang Wenzel, Esther Mittmann, Kersten S. Rabe, Christof M. Niemeyer, Matthias Franzreb, Christof Woell
Summary: We have demonstrated a MOF-based continuous flow enzyme reactor system that allows efficient and stable catalysis in organic solvents. The stability and productivity of the enzyme were significantly improved under aqueous conditions, without the need for additional functionalization, making the fabrication process time- and cost-efficient using label-free enzymes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Engineering, Chemical
Bahram Hosseini Monjezi, Benedikt Sapotta, Sarah Moulai, Jinju Zhang, Robert Oestreich, Bradley P. Ladewig, Klaus Mueller-Buschbaum, Christoph Janiak, Tawheed Hashem, Alexander Knebel
Summary: The metal-organic framework (MOF) MIL-68(In)-NH2 was tested for its applications in dye removal from wastewater and gas separation. Results showed that MIL-68(In)-NH2 exhibited excellent performance in both dye removal and gas separation.
CHEMIE INGENIEUR TECHNIK
(2022)
Article
Engineering, Biomedical
Meik Neufurth, Shunfeng Wang, Heinz C. Schroeder, Bilal Al-Nawas, Xiaohong Wang, Werner E. G. Mueller
Summary: Three-dimensional printing processes are recognized as important fabrication techniques in tissue engineering. This study proposes a bio-ink formulation enriched with polyphosphate (polyP) to increase the viability and migration propensity of mesenchymal stem cells (MSC). The bio-ink also stimulates the growth and differentiation of MSC to mineral depositing osteoblasts.
Article
Infectious Diseases
Reham M. Goda, Ahmed M. El-Baz, Eman M. Khalaf, Nada K. Alharbi, Tarek A. Elkhooly, Mohamed M. Shohayeb
Summary: This study successfully inhibited bacterial colonization on urinary tract catheters by coating them with silver nanoparticles. The silver nanoparticles significantly inhibited the colonization of antibiotic-resistant clinical bacteria and showed stronger antibacterial effects against Gram-negative bacteria.
Article
Biochemistry & Molecular Biology
Heinz C. Schroeder, Xiaohong Wang, Meik Neufurth, Shunfeng Wang, Rongwei Tan, Werner E. G. Mueller
Summary: Two biocatalytically produced inorganic biomaterials, bio-silica and bio-polyphosphate (bio-polyP), have shown great potential in regenerative medicine and other medical applications. Bio-silica is synthesized by a group of enzymes called silicateins, which form amorphous hydrated silica from monomeric precursors. Bio-polyP has morphogenetic activity and can induce cell differentiation, as well as provide metabolic energy through enzymatic cleavage of its high-energy phosphoanhydride bonds. Silicatein and alkaline phosphatase are key enzymes involved in the metabolism and biological activity of these materials.
Article
Materials Science, Multidisciplinary
Werner E. G. Mueller, Hadrian Schepler, Meik Neufurth, Shunfeng Wang, Veronica Ferrucci, Massimo Zollo, Rongwei Tan, Heinz C. Schroeder, Xiaohong Wang
Summary: The polyP hydrogel formulation can accelerate the healing of chronic wounds and provide more metabolic energy by encapsulating and killing bacteria.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Review
Nanoscience & Nanotechnology
Heinz C. Schroeder, Meik Neufurth, Huan Zhou, Shunfeng Wang, Xiaohong Wang, Werner E. G. Mueller
Summary: Inorganic polyphosphates (polyP) are unique polymers that exhibit both morphogenetic and metabolic energy delivering properties, making them of significant medical interest. Understanding the mechanisms of their formation and transformations is crucial for the design of novel materials.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Mousa AlTarabeen, Qosay Al-Balas, Amgad Albohy, Werner Ernst Georg Mueller, Peter Proksch
Summary: Manzamines are a group of compounds extracted from Acanthostrongylophora ingens species. We have identified and characterized seven compounds. Based on previous screening work by Mayer et al., who suggested that manzamine A could be an inhibitor of RSK1 kinase, our work focused on targeting the RSK1 N-terminal kinase domain. Docking using Autodock Vina software showed that Manzamine A N-oxide (5) exhibited the highest activity with an IC50 value of 3.1 μM.
Article
Pharmacology & Pharmacy
Shunfeng Wang, Meik Neufurth, Hadrian Schepler, Rongwei Tan, Zhending She, Bilal Al-Nawas, Xiaohong Wang, Heinz C. Schroeder, Werner E. G. Mueller
Summary: Amorphous calcium carbonate (ACC) precipitated in the presence of inorganic polyphosphate (polyP) has shown potential as a material for bone regeneration. ACC center dot PP particles, stabilized by polyP, have enzymatic activity that promotes the transformation of ACC into crystalline polymorphs. In experiments conducted in vitro and in vivo, ACC center dot PP was found to stimulate cell migration and accelerate wound healing, particularly in chronic wounds.
