Article
Materials Science, Multidisciplinary
Joel Betorz, Gaurav R. Bokil, Shreyas M. Deshpande, Shardool Kulkarni, Daniel R. Araya, Chiara Venturini, Pablo Saez
Summary: In this study, a computational model of cell motility was developed to investigate the mechanisms of cell protrusion, polarization, and retrograde flow. The model was validated through experiments on cell spreading, polarization, and two types of cell migration. Further analysis was done on the effects of chemical and mechanical signals on cell migration, as well as the competition between chemotaxis and durotaxis. The study provides insights into controlling directed cell migration and has implications for tissue and biomedical engineering.
JOURNAL OF THE MECHANICS AND PHYSICS OF SOLIDS
(2023)
Review
Chemistry, Multidisciplinary
Gao-Peng Dang, Wen Qin, Qian-Qian Wan, Jun-Ting Gu, Kai-Yan Wang, Zhao Mu, Bo Gao, Kai Jiao, Franklin R. Tay, Li-Na Niu
Summary: This review discusses the gradient composition of the tendon-bone interface, revealing the regulatory mechanisms and applications, with the aim of creating a mise en scene for future research in interface tissue engineering.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Cell Biology
Jianjun Li, Yufan Liu, Yijie Zhang, Bin Yao, Enhejirigala, Zhao Li, Wei Song, Yuzhen Wang, Xianlan Duan, Xingyu Yuan, Xiaobing Fu, Sha Huang
Summary: This review emphasizes the importance of understanding the biophysical and biochemical cues in guiding mesenchymal stem cell (MSC) behaviors, as well as integrating these cues into biomaterials to enhance the prospects of MSC applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Markus Rimann, Astrid Juengel, Sara Mousavi, Nicole Moeschlin, Maurizio Calcagni, Karin Wuertz-Kozak, Florian Brunner, Stefan Dudli, Oliver Distler, Christian Adlhart
Summary: This study demonstrated the suitability of two synthetic polymers in creating electrospun scaffolds for 3D skin cell models, with P(AN-MA) mats showing enhanced cell compatibility and infiltration compared to Pul/PVA/PAA mats.
Article
Engineering, Biomedical
Shengran Wang, Changlai Zhu, Bin Zhang, Junxia Hu, Jinghui Xu, Chengbin Xue, Shuangxi Bao, Xiaokun Gu, Fei Ding, Yumin Yang, Xiaosong Gu, Yun Gu
Summary: A favorable microenvironment is crucial for nerve regeneration. This study investigates the components and spatial organization of extracellular matrix (ECM) secreted by different cells and finds that ECM derived from bone marrow mesenchymal cells (BMSCs) is more similar to acellular nerves. ECM-modified nerve grafts (ECM-NGs) co-cultivated with BMSCs exhibit the most promising nerve repair properties and create a superior microenvironment for nerve regeneration, indicating a potential alternative for clinical repair of peripheral nerve defects.
Article
Cell Biology
Panpan Xu, Jingjing Guan, Yu Chen, Hui Xiao, Tianhao Yang, Hengheng Sun, Nan Wu, Changchun Zhang, Yingji Mao
Summary: This study evaluated the encapsulation of nucleus pulposus cells (NPCs) in GelMA hydrogel to improve intervertebral disc degeneration. Adjusting the concentration of GelMA affected the mechanical properties and viability of the encapsulated NPCs. The controllability of GelMA hydrogels makes them promising candidates for NP tissue engineering and repairing endogenous NPCs.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Review
Biotechnology & Applied Microbiology
Rebecca Powell, Despoina Eleftheriadou, Simon Kellaway, James B. Phillips
Summary: This review discusses the physical processes by which natural biomaterials mimic the function of the extracellular matrix and regulate cellular behavior, as well as highlights representative cases of controllable cell microenvironments developed through the combination of cell biology and tissue engineering principles.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Jing Xie, Min Bao, Xinyu Hu, Werner J. H. Koopman, Wilhelm T. S. Huck
Summary: Cells respond to mechanical cues from the extracellular matrix by adjusting their cytoskeleton and forming focal adhesions, which can lead to changes in ATP levels and activation of AMPK. This can affect cell spreading and tension, as well as influence stem cell fate in response to ECM mechanics. Lowered ATP levels on softer substrates limit cellular mechanoresponses.
Article
Engineering, Biomedical
Zhichao Hao, Lin Ren, Zhen Zhang, Zaiwu Yang, Shujie Wu, Gen Liu, Bin Cheng, Jun Wu, Juan Xia
Summary: Recently, it has been shown that it is important to reproduce the bone microenvironment for bone regeneration. This study developed a neural tissue engineering hydrogel-encapsulated system using Schwann cell-derived exosomes (SC Exo) that were able to enhance bone regeneration by promoting innervation, immunoregulation, vascularization, and osteogenesis. In addition, this system also induced M2 polarization of macrophages, tube formation of HUVECs, and BMSCs osteogenic differentiation in vitro, and promoted BMSCs osteogenesis through the TGF-beta 1/ SMAD2/3 signaling pathway.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Shan Su, Yueshun Qiu, Xingxu Shi, Teng Zhou, Peng Wang, E. Xiao, Qiang Wei, Changsheng Zhao
Summary: Stem cell therapy is advancing quickly, however, current expansion techniques are inadequate for large-scale cell use. The surface chemistry and morphology of materials are crucial for cell behaviors and functions, guiding the design of biomaterials. Cell adhesion and growth are affected by these factors, making the design of a suitable biomaterial interface a major focus of recent research.
Review
Physiology
Seth M. Woodbury, W. Benton Swanson, Yuji Mishina
Summary: Skeletal stem and progenitor cells (SSPCs) are versatile cells that form the hematopoietic environment and adventitial structures in skeletal tissues. The differentiation of SSPCs is influenced by the physical properties of their microenvironment and mechanical forces within the skeletal system.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Engineering, Biomedical
Jose L. Cadavid, Simon Latour, Ferris Nowlan, Ileana L. Co, Natalie Landon-Brace, Bradly G. Wouters, Barbara T. Grunwald, Mark Nitz, Hartland Warren Jackson, Alison P. McGuigan
Summary: The spatial configuration of cells in the tumor microenvironment affects cancer and fibroblast cell phenotypes, leading to the clinical challenges of tumor heterogeneity and therapeutic resistance. In this study, a versatile system using pancreatic ductal adenocarcinoma as a model is described to investigate the impact of tissue architecture on cell phenotype. High dimensional characterization using CyTOF reveals distinct hypoxia and proliferation gradients in tissue architecture, and phenotypic markers for both cell types are also graded in a way that cannot be explained by hypoxia or coculture alone.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Jialun Cai, Junjuan Wang, Chenxuan Sun, Jianwu Dai, Can Zhang
Summary: Interface tissue engineering is a rapidly growing field focused on developing engineered tissue replacements to promote integration between multiple tissue types by using biomaterials with specific compositions and stiffness gradients to regulate cellular behavior and tissue regeneration.
BIOMEDICAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Michaela Petaroudi, Aleixandre Rodrigo-Navarro, Oana Dobre, Matthew J. Dalby, Manuel Salmeron-Sanchez
Summary: Living interfaces are a novel class of active materials that allow precise control over cell behavior. This study presents a biointerface between engineered bacteria and mammalian cells, which successfully regulates the stemness markers of human mesenchymal stem cells.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Engineering, Biomedical
Peng Guo, Xizhe Liu, Penghui Zhang, Zhongyuan He, Zhen Li, Mauro Alini, R. Geoff Richards, Sibylle Grad, Martin J. Stoddart, Guangqian Zhou, Xuenong Zou, Danny Chan, Wei Tian, Dafu Chen, Manman Gao, Zhiyu Zhou, Shaoyu Liu
Summary: This study maps the transcriptional landscapes of different osteogenic microenvironments for mesenchymal stromal cells (MSCs) at single-cell resolution, providing insights into the regulation of bone regeneration by three-dimensional hydroxyapatite (HA) scaffolds and osteogenic medium (OM). The research reveals that HA scaffolds activate osteogenic-related signaling pathways and inhibit adipogenesis and fibrosis, while OM mediates osteogenesis through the ZBTB16 and WNT signaling pathways. The study also demonstrates the heterogeneity of MSCs and provides new directions for improving osteogenic biomaterials.
BIOACTIVE MATERIALS
(2022)
Article
Hematology
Terence Gee, Emily Farrar, Yidong Wang, Bingruo Wu, Kevin Hsu, Bin Zhou, Jonathan Butcher
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2020)
Article
Cardiac & Cardiovascular Systems
Kiran A. Vaidya, Matthew P. Donnelly, Terence W. Gee, Marine-Ayan Ibrahim Aibo, Stephen Byers, Jonathan T. Butcher
CARDIOVASCULAR PATHOLOGY
(2020)
Article
Multidisciplinary Sciences
Maria C. Palumbo, Lisa Q. Rong, Jiwon Kim, Pedram Navid, Razia Sultana, Jonathan Butcher, Alberto Redaelli, Mary J. Roman, Richard B. Devereux, Leonard N. Girardi, Mario F. L. Gaudino, Jonathan W. Weinsaft
Article
Biophysics
Huseyin Enes Salman, Maha Alser, Akshay Shekhar, Russell A. Gould, Fatiha M. Benslimane, Jonathan T. Butcher, Huseyin C. Yalcin
Summary: This study investigates disturbed hemodynamics and deteriorated cardiac growth following left atrial ligation (LAL) to explore potential mechanobiological mechanisms for the embryonic development of hypoplastic left heart syndrome (HLHS), highlighting the critical importance of hemodynamic disturbances during heart valve and ventricle development.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shaohua Wu, Vikas Kumar, Peng Xiao, Mitchell Kuss, Jung Yul Lim, Chittibabu Guda, Jonathan Butcher, Bin Duan
SCIENTIFIC REPORTS
(2020)
Article
Engineering, Biomedical
Terence W. Gee, Jennifer M. Richards, Ablajan Mahmut, Jonathan T. Butcher
Summary: This study utilized a mechanically stressed 3D culture system to replicate valve interstitial cell-induced matrix calcification and investigated the collaborative interactions between valve endothelial cells and interstitial cells in calcific pathogenesis. Results showed that endothelial cells induce pathological remodeling and calcification of interstitial cells through EndMT and paracrine signaling.
Review
Cardiac & Cardiovascular Systems
Katherine Driscoll, Alexander D. Cruz, Jonathan T. Butcher
Summary: Calcific aortic valve disease is on the rise globally, but treatment is limited to prosthetic replacement. With a growing number of younger and more active patients, alternative therapies are needed. There is potential for biologically based diagnostics and treatments to help alleviate or delay disease progression, but a limited understanding of the disease mechanisms hinders progress towards clinical interventions.
CIRCULATION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Michael F. Z. Wang, Madhav Mantri, Shao-Pei Chou, Gaetano J. Scuderi, David W. McKellar, Jonathan T. Butcher, Charles G. Danko, Iwijn De Vlaminck
Summary: Conventional single-cell RNA sequencing analysis relies on genome annotations that may be incomplete or inaccurate especially for understudied organisms. Here the authors present a bioinformatic tool that leverages single-cell data to uncover biologically relevant transcripts beyond the best available genome annotation.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Madhav Mantri, Gaetano J. Scuderi, Roozbeh Abedini-Nassab, Michael F. Z. Wang, David McKellar, Hao Shi, Benjamin Grodner, Jonathan T. Butcher, Iwijn De Vlaminck
Summary: By utilizing single-cell and spatial transcriptomics in chicken hearts, the authors conducted a census of cellular interactions from early to late four-chambered heart stage, identifying a distinct epicardial-mesenchymal cell population with a migratory phenotype.
NATURE COMMUNICATIONS
(2021)
Article
Engineering, Biomedical
Stephanie E. Lindsey, Irene E. Vignon-Clementel, Jonathan T. Butcher
Summary: Mechanical forces play a crucial role in the growth and remodeling of primitive pharyngeal arch arteries into the heart's great vessels. However, the direct correlation between hemodynamics and PAA morphogenesis has been poorly understood. A study using minimally invasive occlusion experiments in avian embryos and 3D anatomical models revealed significant changes in morphology and hemodynamics following vessel occlusion, providing insights into the dynamic vessel adaptation process.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Developmental Biology
David Bassen, Mingkun Wang, Duc Pham, Shuofei Sun, Rashmi Rao, Rishabh Singh, Jonathan Butcher
Summary: This study investigated the influence of compressive and tensile stresses on valve growth and shape maturation, identifying a mechanically activated switch between valve growth and maturation. Compressive stress was found to drive cell proliferation via BMP signaling, while tensile stress promoted valve maturation through MLC2 contractility. The effects of osmotic stress on valve development were shown to be conserved through different stages.
Article
Anatomy & Morphology
Duc H. Pham, Charles R. Dai, Belle Y. Lin, Jonathan T. Butcher
Summary: The study found that the elongation of the fetal semilunar valve occurs through fibrosa-sided endocardial proliferation. Low shear stress induces canonical Wnt signaling in fetal valve endothelium, driving endocardial proliferation, while high shear stress activates Notch signaling to inhibit Wnt signaling. This novel mechanically regulated molecular switch explains how fluid shear stress guides the growth direction of valve endothelium.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Engineering, Biomedical
Nianfang Ma, Daniel Y. Cheung, Jonathan T. Butcher
Summary: In this study, a novel photocrosslinkable composite hydrogel mNCC-MeGel (mNG) was fabricated and evaluated for heart valve applications. Encapsulated HADMSC in mNG showed support for a quiescent fibroblastic phenotype and resistance towards calcification, making it an attractive biomaterial for TEHV. Additionally, mNG was successfully used for 3D bioprinting a tall tubular structure that sustained cell viability, demonstrating its potential for tissue engineering.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Multidisciplinary Sciences
Emily J. Farrar, Emilye Hiriart, Ablajan Mahmut, Bernd Jagla, David S. Peal, David J. Milan, Jonathan T. Butcher, Michel Puceat
Summary: Studies have found that a subset of mouse endocardial cells express Oct4 induced by nuclear factor kappa B (NF kappa B) during the process of transition starting at day 9.5 of embryo development. These cells acquire a chondro-osteogenic fate and in adulthood lead to valve calcification. Genetic deletion of OCT4 can prevent aortic stenosis and valve calcification.
Correction
Engineering, Biomedical
Sraeyes Sridhar, Duc H. Pham, Terence W. Gee, Joanna Hua, Jonathan T. Butcher
CURRENT OPINION IN BIOMEDICAL ENGINEERING
(2021)
