Article
Dermatology
Burcu Beksac, Laura Gleason, Sarah Baik, John M. Ringe, Pierluigi Porcu, Neda Nikbakht
Summary: This study investigated the effect of dermal fibroblasts and fibronectin on the survival and proliferation of malignant cutaneous lymphoma cells. The results showed that dermal fibroblasts increased the proliferation rates of the lymphoma cells but did not rescue them from apoptosis. Additionally, an overexpression of a fibronectin isoform was found in the lymphoma skin. The malignant cells expressed fibronectin-binding integrins and adhered to fibronectin, but adhesion-mediated survival was not observed. These findings have important implications for understanding and targeting the malignant skin stromal microenvironment in cutaneous lymphomas.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2022)
Article
Engineering, Biomedical
Agustina Setiawati, Dongyoon Jang, Daeyon Cho, Shingyu Cho, Hayan Jeong, Sungmin Park, Jungsug Gwak, Soo Ryeon Ryu, Won Hee Jung, Bong-Gun Ju, Kwang-Hwan Jung, Oh-Sun Kwon, Kwanwoo Shin
Summary: The research introduces a new method to coat absorbable sutures with fibronectin, promoting cellular migration and adhesion while preventing infection. The modified suture also reduces friction with wounded tissue, enhancing overall healing outcomes.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Xian Xie, Zhuo Li, Xuefeng Yang, Boguang Yang, Zhixian Zong, Xuemei Wang, Liting Duan, Sien Lin, Gang Li, Liming Bian
Summary: In this study, a peptide fiber-reinforced hydrogel was developed, where the stiff β-sheet peptide fiber acted as a multivalent cross-linker to enhance the macroscopic stability of the hydrogel. The dynamic imine cross-link between the peptide fiber and polymer network endowed the hydrogel with a microscopically dynamic network. The resulting fibrillar nanocomposite hydrogel, with its cell-adaptable dynamic network, significantly enhanced cell-matrix and cell-cell interactions, promoting mechanotransduction, metabolic energetics, and osteogenesis of encapsulated stem cells. Additionally, the hydrogel could deliver a fiber-attached inductive drug to further enhance bone regeneration. This work provides valuable guidance for the design of cell-adaptive and bioactive biomaterials for therapeutic applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Engineering, Biomedical
Minjeong Jang, Seung Won Oh, Yunji Lee, Jin Young Kim, Eun Sun Ji, Pilnam Kim
Summary: In this study, the researchers found that accumulated AGEs in the ECM can alter the phenotype of fibroblast within a three-dimensional collagen matrix, leading to the acquisition of a cancer-associated fibroblast (CAF)-like phenotype. These effects can be blocked by neutralizing antibodies against the AGE receptor (RAGE) or by inhibiting focal adhesion signaling. In addition, an AGE cross-link breaker called ALT-711 can reduce the transformation of fibroblasts into the CAF-like phenotype. Decreased matrix stiffness also attenuates fibroblast activation. These findings suggest potential therapeutic strategies targeting non-enzymatic ECM glycation to regulate fibroblast activation.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Meng Zhang, Fenghua Zhao, Xue Zhang, Linda A. Brouwer, Janette K. Burgess, Martin C. Harmsen
Summary: This study found that MRC-5 fibroblasts promote collagen fiber remodeling and deposition of fibronectin in the hydrogel, leading to modifications in the ECM microenvironment and promoting vascularization.
MATERIALS TODAY BIO
(2023)
Article
Biotechnology & Applied Microbiology
Subhajit Konar, Scott M. Bolam, Brendan Coleman, Nicola Dalbeth, Sue R. McGlashan, Sophia Leung, Jillian Cornish, Dorit Naot, David S. Musson
Summary: Tendinopathy is characterized by pathological changes in tendon matrix composition, architecture, and stiffness, with inflammation also playing an important role. This study found that substrate stiffness affects tendon-derived cells and macrophages. Tendon-derived cells showed minor responses to substrate stiffness, while macrophages exhibited a more inflammatory phenotype on non-physiological stiffness substrates. These subtle variations in matrix stiffness may contribute to the onset and progression of tendinopathy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Eun-Ju Lee, Khurshid Ahmad, Shiva Pathak, SunJu Lee, Mohammad Hassan Baig, Jee-Heon Jeong, Kyung-Oh Doh, Dong-Mok Lee, Inho Choi
Summary: This study introduces novel peptides derived from fibronectin that enhance cell adhesion, proliferation, and differentiation, offering a new approach to cell culture. The peptides showed significant effects on primary cells and stem cells, increasing proliferation and promoting differentiation into specific cell types such as osteoblasts. The results suggest potential applications in the production of core materials for cell culture such as scaffolds and culture medium components.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yi-Kai Hong, Chen-Han Wu, Yu-Chen Lin, Yu-Lun Huang, Kuo-Shu Hung, Tsung-Pin Pai, Yen-Ting Liu, Tzu-Chi Chen, Hardy Chan, Chao-Kai Hsu
Summary: This study demonstrated that ASC-J9 treatment of keloid fibroblasts can inhibit the activation of fibroblasts, reduce cell division, inflammation, ROS generation, and extracellular matrix synthesis, as well as suppress IL-6 protein secretion. Inhibiting the ROS/STAT3/IL-6 axis by ASC-J9 may represent a potential therapeutic approach to suppress cell proliferation and ECM production in keloids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Karen G. Soans, Ana Patricia Ramos, Jaydeep Sidhaye, Abhijeet Krishna, Anastasia Solomatina, Karl B. Hoffmann, Raimund Schluessler, Jochen Guck, Ivo F. Sbalzarini, Carl D. Modes, Caren Norden
Summary: Cell migration is crucial for organismal development, but the influence of physical properties on migration phenomena in vivo remains unclear. In this study, the researchers used zebrafish optic cup formation to investigate the role of extracellular matrix (ECM) properties in cell migration. They found that rim cells migrate over an immobile ECM and require cryptic lamellipodia for movement. The topology of the ECM changes along the migration path and is accompanied by changes in cell-matrix interactions. Matrix porosity is linked to efficient migration.
Article
Engineering, Biomedical
Sang Woo Lee, Se Eun Park, Gi Seok Jeong
Summary: The study found that MCTs-ECM tumor grafts promote high activity of MMP2 and MMP9, increasing cancer cell motility and showing enhanced drug resistance after anticancer drug treatment, further activating cancer cell motility, providing a useful macroscopic tumor model for evaluating the efficacy of anticancer drugs targeting aggressive cancer cells.
Article
Cardiac & Cardiovascular Systems
Matthew C. Watson, Corin Williams, Raymond M. Wang, Luke R. Perreault, Kelly E. Sullivan, Whitney L. Stoppel, Lauren D. Black III
Summary: This study aimed to determine the proliferation and maturation potential of cardiomyocytes from hypoplastic hearts and investigate whether these behaviors are influenced by biochemical signaling from the extracellular matrix and cyclic mechanical stretch.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Oncology
Qiang Huang, Chi-Yao Hsueh, Yu-Jie Shen, Yang Guo, Jia-Meng Huang, Yi-Fan Zhang, Jiao-Yu Li, Hong-Li Gong, Liang Zhou
Summary: The study demonstrates that TGFS1 packaged in sEV can reprogram NFs into CAFs, shedding light on an important mechanism for cancer-recruited stroma responses.
Review
Immunology
Lucas Albacete-Albacete, Miguel Sanchez-alvarez, Miguel Angel del Pozo
Summary: ECM composition and architecture are tightly regulated, with diseases associated to alterations in the levels of proteins such as TnC. EV-dependent ECM secretion may be a key mechanism underlying diseases related to ECM integrity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Jill T. Kuwabara, Akitoshi Hara, Sumit Bhutada, Greg S. Gojanovich, Jasmine Chen, Kanani Hokutan, Vikram Shettigar, Anson Y. Lee, Lydia P. DeAngelo, Jack R. Heckl, Julia R. Jahansooz, Dillon K. Tacdol, Mark T. Ziolo, Suneel S. Apte, Michelle D. Tallquist
Summary: Reducing fibroblasts in the heart has a protective effect and can alleviate cardiac fibrosis after injury. The loss of fibroblasts does not significantly alter the quantitative composition of the extracellular matrix (ECM) but reduces the distribution and quantity of collagen VI. This study has important implications for the treatment of heart disease.
Article
Cardiac & Cardiovascular Systems
Michael Warkala, Dongying Chen, AnnJosette Ramirez, Ali Jubran, Michael Schonning, Xia Wang, Huaning Zhao, Sophie Astrof
Summary: The development of the fourth pharyngeal arch arteries involves a multistep process with contributions from SHF-derived ECs, remodeling of endothelial plexus into PAAs, and remodeling of PAAs into the aortic arch artery and its major branches. Cell-ECM interactions regulated by integrin alpha 5 beta 1 and Fn1 are essential at each developmental stage.
CIRCULATION RESEARCH
(2021)
Article
Engineering, Biomedical
Soichi Iwai, Satoko Kishimoto, Yuto Amano, Akihiro Nishiguchi, Michiya Matsusaki, Akinori Takeshita, Mitsuru Akashi
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2019)
Article
Chemistry, Multidisciplinary
Sarah Bertlein, Daichi Hikimoto, Gernot Hochleitner, Julia Huemmer, Tomasz Jungst, Michiya Matsusaki, Mitsuru Akashi, Jurgen Groll
Article
Cell & Tissue Engineering
Maki Takeda, Shigeru Miyagawa, Satsuki Fukushima, Atsuhiro Saito, Emiko Ito, Akima Harada, Ryohei Matsuura, Hiroko Iseoka, Nagako Sougawa, Noriko Mochizuki-Oda, Michiya Matsusaki, Mitsuru Akashi, Yoshiki Sawa
TISSUE ENGINEERING PART C-METHODS
(2018)
Article
Biotechnology & Applied Microbiology
Sho Shinohara, Takanori Kihara, Shinji Sakai, Michiya Matsusaki, Mitsuru Akashi, Masahito Taya, Jun Miyake
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2013)
Article
Biochemistry & Molecular Biology
Masahiro Matsumoto, Michiya Matsusaki, Mitsuru Akashi
MACROMOLECULAR BIOSCIENCE
(2014)
Article
Chemistry, Analytical
Muhammad Asri Abdul Sisak, Fiona Louis, Sun Hyeok Lee, Young-Tae Chang, Michiya Matsusaki
Review
Engineering, Chemical
Ghmkin Hassan, Said M. Afify, Shiro Kitano, Akimasa Seno, Hiroko Ishii, Yucheng Shang, Michiya Matsusaki, Masaharu Seno
Summary: Cancer stem cells (CSCs) play a crucial role in drug resistance and relapse, and utilizing biomaterials to mimic the microenvironment can aid in understanding their behavior and identifying specific CSCs in different types of cancer. This technology holds promise in simplifying the complexity of the microenvironment and providing insights for future drug screening.
Article
Chemistry, Analytical
Satoko Sasaki, Tomoko Suzuki, Kyojiro Morikawa, Michiya Matsusaki, Kae Sato
Summary: This study introduces a new technique for fabricating microfluidic devices using microbial transglutaminase-gelatin gels instead of PDMS. The gelatin-based device with a coverslip as the bottom allows high-magnification observations and has been shown to have different cell dynamics compared to glass and PDMS surfaces. Co-culturing human umbilical vein endothelial cells (HUVECs) and normal human dermal fibroblasts (NHDFs) resulted in blood vessel-like structures, and gelatin was found to be a suitable substrate for cell adhesion. The device's ability for high-magnification microscopic observation and easy sample introduction make it more convenient than traditional gelatin microfluidics.
Article
Chemistry, Analytical
Huiting Zhang, Dong-Hee Kang, Marie Piantino, Daisuke Tominaga, Takashi Fujimura, Noriyuki Nakatani, J. Nicholas Taylor, Tomomi Furihata, Michiya Matsusaki, Satoshi Fujita
Summary: The blood-brain barrier (BBB) is a selective barrier that controls transport between the blood and neural tissue. Researchers have developed a 3D BBB model that replicates key features of the native BBB, allowing for the study of disease and drug delivery effects. They have also developed a non-invasive approach using optical coherence tomography (OCT) to quantify the microvessel network in the BBB model.
Article
Biology
Kiminori Yanagisawa, Masamitsu Konno, Hao Liu, Shinji Irie, Tsunekazu Mizushima, Masaki Mori, Yuichiro Doki, Hidetoshi Eguchi, Michiya Matsusaki, Hideshi Ishii
Meeting Abstract
Oncology
Michiya Matsusaki
Meeting Abstract
Oncology
Soichi Iwai, Satoko Kishimoto, Kyoko Nishiyama, Akinori Takeshita, Masakazu Hamada, Michiya Matsusaki, Mitsuru Akashi
Article
Chemistry, Multidisciplinary
Sooyeon Kim, Yang Zhou, Norimitsu Tohnai, Hirotaka Nakatsuji, Michiya Matsusaki, Mamoru Fujitsuka, Mikiji Miyata, Tetsuro Majima
CHEMISTRY-A EUROPEAN JOURNAL
(2018)
Article
Biochemistry & Molecular Biology
Yoshiya Asano, Tadashi Odagiri, Hiroe Oikiri, Michiya Matsusaki, Mitsuru Akashi, Hiroshi Shimoda
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)
Article
Engineering, Biomedical
Yu Bin Lee, Joong-yup Lee, Hayeon Byun, Taufiq Ahmad, Mitsuru Akashi, Michiya Matsusaki, Heungsoo Shin