期刊
ACTA BIOMATERIALIA
卷 8, 期 9, 页码 3313-3325出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2012.05.023
关键词
Biomimetic material; Intervertebral disc tissue engineering; Silk; Extracellular matrix; Fibrous scaffold
资金
- Indo-Swiss Joint Research Programme [ISJRP 123 003]
- Indian Council of Medical Research project [09/NCD-1]
- Department of Biotechnology, Government of India
A novel design of silk-based scaffold is developed using a custom-made winding machine, with fiber alignment resembling the anatomical criss-cross lamellar fibrous orientation features of the annulus fibrosus of the intervertebral disc. Crosslinking of silk fibroin fibers with chondroitin sulphate (CS) was introduced to impart superior biological functionality. The scaffolds, with or without CS, instructed alignment of expanded human chondrocytes and of the deposited extracellular matrix while supporting their chondrogenic redifferentiation. The presence of CS crosslinking could not induce statistically significant changes in the measured collagen or glycosaminoglycan content, but resulted in an increased construct stiffness. By offering the combined effect of cell/matrix alignment and chondrogenic support, the silk fibroin scaffolds developed with precise fiber orientation in lamellar form represent a suitable substrate for tissue engineering of the annulus fibrosus part of the intervertebral disc. Crown Copyright (C) 2012 Published by Elsevier Ltd. on behalf of Acta Materialia Inc. All rights reserved.
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