Article
Materials Science, Biomaterials
Ling Zhang, Na Xiong, Yanfei Liu, Lili Gan
Summary: The study demonstrates that incorporating cell-adhesive ligands in 3D self-assembling peptide hydrogels is effective for maintaining stem cell survival, proliferation, and enhancing osteogenic differentiation. The composite hydrogels also upregulate the expression of pluripotent markers in stem cells, indicating their potential for modulating cell fate.
REGENERATIVE BIOMATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Ting-Wei Kao, Arthur Chiou, Keng-Hui Lin, Yi-Shiuan Liu, Oscar Kuang-Sheng Lee
Summary: Research shows that mechanical stimulation affects the intracellular viscoelasticity of human mesenchymal stem cells, with cells cultured in rigid matrix displaying higher elastic and viscous moduli. This effect remains consistent throughout the differentiation process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Shiqi Xiang, Zhong Li, Madalyn R. Fritch, La Li, Sachin Velankar, Yuwei Liu, Jihee Sohn, Natasha Baker, Hang Lin, Rocky S. Tuan
Summary: This study investigates the adipogenic differentiation of hBMSCs within soft or stiff scaffolds in 3D culture, with a focus on the molecular mechanism involving the CAV1-YAP axis. Soft biomaterials are found to better support adipogenesis of encapsulated hBMSCs, partially mediated by suppression of CAV1 expression levels and activation of YAP signaling pathway.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Pharmacology & Pharmacy
Jeevithan Elango
Summary: This study developed a novel hydrogel system using collagen, chitosan, and VEGF composites for culturing MSCs and investigated their ability for osteogenic differentiation and mineral deposition. The hydrogel loaded with 100 ng/mL VEGF showed significant support for MSC proliferation, fibrillary filament structure, mineralization, alkaline phosphatase, and osteogenesis compared to other hydrogels and control. The hydrogel with VEGF could be used as an appropriate system to culture stem cells for bone and dental regeneration.
Article
Multidisciplinary Sciences
Sook-Kyoung Heo, Yunsuk Choi, Yoo Kyung Jeong, Lan Jeong Ju, Ho-Min Yu, Do Kyoung Kim, Hye Jin Seo, Yoo Jin Lee, Jaekyung Cheon, SuJin Koh, Young Joo Min, Eui-Kyu Noh, Jae-Cheol Jo
Summary: Osteoporosis is a progressive skeletal disease affecting millions worldwide, currently treated with drugs that have severe side effects. Cell replacement therapy using mesenchymal stem cells may be a future treatment strategy. This study found that LIGHT can enhance osteogenesis of hBM-MSCs, possibly through WNT/β-catenin pathway activation.
Review
Biochemistry & Molecular Biology
Parisa Khayambashi, Janaki Iyer, Sangeeth Pillai, Akshaya Upadhyay, Yuli Zhang, Simon D. Tran
Summary: Tissue engineering has a long history in regenerative medicine, and methods such as cell-encapsulated hydrogels and exosomes can enhance the regenerative potential of various tissues, with hydrogels being the most user-friendly, economical, and accessible material for encapsulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Shiva Shariati, Ehsan Seyedjafari, Fatemeh Sadat Mahdavi, Amirhosein Maali, Elaheh Ferdosi-Shahandashti
Summary: In this study, NiFe2O4/ZnO-coated PLLA scaffold was used to evaluate the osteogenic differentiation capability of hAMSCs. The results showed that the NiFe2O4/ZnO-coated PLLA scaffold could enhance the adhesion, proliferation, and differentiation of hAMSCs, as well as promote the expression of osteogenic markers.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Medicine, Research & Experimental
Leiluo Yang, Qing Li, Junhong Zhang, Pengcheng Li, Pingjiang An, Chunqing Wang, Pingsheng Hu, Xue Zou, Xiaowei Dou, Lixin Zhu
Summary: The study investigated the role of Wnt7a in bone formation and demonstrated its key role in MSC differentiation into osteoblasts, offering a potential therapeutic target for bone-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Behnaz Sadat Eftekhari, Dawei Song, Paul A. Janmey
Summary: Electrical stimulation of human mesenchymal stem cells cultured on soft conductive biodegradable substrates promotes neurite outgrowth and upregulates neuronal markers while downregulating glial markers. The combination of soft conductive substrates and electrical stimulation is a promising tool for enhancing neuronal tissue engineering outcomes.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Cell & Tissue Engineering
Rongmei Qu, Kai He, Tingyu Fan, Yuchao Yang, Liyao Mai, Zhiwei Lian, Zhitao Zhou, Yan Peng, Asmat Ullah Khan, Bing Sun, Xiaolan Huang, Jun Ouyang, Xinghua Pan, Jingxing Dai, Wenhua Huang
Summary: The study used single-cell RNA sequencing to investigate the chemically induced osteogenesis process of human adipose MSCs, identifying some cells intrinsically prepared for osteogenesis and a set of genes related to cell stress response.
Article
Polymer Science
Pierre C. Dromel, Deepti Singh, Alfredo Alexander-Katz, Motoichi Kurisawa, Myron Spector, Michael Young
Summary: Engineering matrices for cell therapy require design criteria that include the ability of these materials to support, protect, and enhance cellular behavior in vivo. The chemical and mechanical formulation of the biomaterials can influence not only target cell phenotype but also cellular differentiation. By manipulating the stiffness of the hydrogel, the differentiation potential of human retinal progenitor cells can be controlled.
Review
Polymer Science
Aiah A. El-Rashidy, Sara El Moshy, Israa Ahmed Radwan, Dina Rady, Marwa M. S. Abbass, Christof E. Doerfer, Karim M. Fawzy El-Sayed
Summary: The stiffness of the extracellular matrix plays a regulatory role in directing the osteogenic differentiation of MSCs, with MSCs sensing and responding to their ECM to undergo differentiation. Customizing scaffold materials to mimic cells' natural environment is crucial in the tissue engineering process.
Article
Chemistry, Applied
Han-Sem Kim, JunHwee Jang, Jun-Sung Oh, Eun-Jung Lee, Cheol-Min Han, Ueon Sang Shin
Summary: In this study, alendronate was tethered to an AlA to form AlACa, and mixed with alginate to create AlAA hydrogel. The hydrogel exhibited appropriate gelation time and mechanical stiffness, promoting cellular proliferation and differentiation. It has potential as a scaffold in bone tissue engineering.
CARBOHYDRATE POLYMERS
(2023)
Article
Cell Biology
Yoon Young Go, Sung-won Chae, Jae-Jun Song
Summary: Human chorionic membrane extracts (CME) enhance osteogenesis by providing an osteoconductive environment for osteoblasts. CME contains various osteogenesis-related stimulators and triggers osteogenic differentiation through specific cellular signaling pathways.
CELL PROLIFERATION
(2022)
Article
Anatomy & Morphology
Li Hua, Xiaohong Zhang
Summary: The study revealed that MALAT1 modulated differentiation of human periodontal ligament stem cells by regulating miR155-5p during osteogenic differentiation.
Article
Cell & Tissue Engineering
Jeayoung Park, Petr Baranov, Aybike Aydin, Hany Abdelgawad, Deepti Singh, Wanting Niu, Motoichi Kurisawa, Myron Spector, Michael J. Young
CELL TRANSPLANTATION
(2019)
Article
Engineering, Biomedical
Shengyong Ng, Wai Jin Tan, Michelle Mi Xue Pek, Min-Han Tan, Motoichi Kurisawa
Article
Clinical Neurology
Teck Chuan Lim, Emiri Mandeville, Ding Weng, Li-Shan Wang, Motoichi Kurisawa, Kimberly Leite-Morris, Magdy H. Selim, Eng H. Lo, Myron Spector
TRANSLATIONAL STROKE RESEARCH
(2020)
Article
Materials Science, Multidisciplinary
Pierre Colombe Dromel, Deepti Singh, Alfredo Alexander-Katz, Motoichi Kurisawa, Myron Spector, Michael Young
APPLIED MATERIALS TODAY
(2020)
Article
Cell & Tissue Engineering
Abdulmonem Alshihri, Wanting Niu, Peer Kaemmerer, Mansour Al-Askar, Atsushi Yamashita, Motoichi Kurisawa, Myron Spector
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Review
Engineering, Biomedical
Shengyong Ng, Motoichi Kurisawa
Summary: Lab-grown or cultured meat has garnered attention but faces obstacles in replicating realistic meat tissues and scaling up production efficiently. By understanding the molecular basis of desirable meat properties and the role of food biopolymers in mimicking these properties, efforts are made to bridge the fields of meat science and biomaterials engineering to inspire strategies addressing these challenges.
ACTA BIOMATERIALIA
(2021)
Article
Polymer Science
Ki Hyun Bae, Kiat Hwa Chan, Motoichi Kurisawa
Summary: This study reports the synthesis of poly(acrylic acid)-EGCG conjugates that self-assemble into micellar nanoparticles with enhanced resistance against autoxidation. These nanoparticles not only exhibit superior stability and cytocompatibility, but also show excellent ROS-scavenging and anti-inflammatory effects.
Article
Biochemistry & Molecular Biology
Ki Hyun Bae, Fritz Lai, Betul Oruc, Motomi Osato, Qingfeng Chen, Motoichi Kurisawa
Summary: The article describes a micellar nanocomplex formed from EGCG and daunorubicin, which can release the drug in an acidic environment. It has better cytotoxic effect on multidrug-resistant leukemia cells and enhances the accumulation of the drug in the nucleus, increases intracellular reactive oxygen species, and induces caspase-mediated apoptosis. This provides a promising strategy for overcoming chemoresistance in leukemia therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Sijing Xiong, Susi Tan, Peng Huang, Yao Li, Joo Eun Chung, Motoichi Kurisawa, Daniele Zink, Jackie Y. Y. Ying
Summary: Nanoscale systems for anticancer drug delivery can reduce toxicity towards non-tumor cells. Micellar nanocomplexes (MNCs) comprising green tea catechin derivatives have been developed for efficient delivery of anticancer proteins. MNCs showed potent cytotoxicity against breast cancer cells, regardless of HER2/neu expression levels, while they were not toxic for normal human cells. These findings suggest that green tea catechin derivative-based MNCs could improve the efficacy and safety of therapies with anticancer proteins.
BIOMATERIALS SCIENCE
(2023)
Article
Polymer Science
Pierre C. Dromel, Deepti Singh, Alfredo Alexander-Katz, Motoichi Kurisawa, Myron Spector, Michael Young
Summary: Engineering matrices for cell therapy require design criteria that include the ability of these materials to support, protect, and enhance cellular behavior in vivo. The chemical and mechanical formulation of the biomaterials can influence not only target cell phenotype but also cellular differentiation. By manipulating the stiffness of the hydrogel, the differentiation potential of human retinal progenitor cells can be controlled.
Article
Chemistry, Multidisciplinary
Fan Lee, Ki Hyun Bae, Shengyong Ng, Atsushi Yamashita, Motoichi Kurisawa
Summary: The study found that hyaluronic acid-EGCG conjugates have the potential as an anti-arthritic agent, targeting fibroblast-like synoviocytes, and exhibiting superior anti-proliferative and anti-inflammatory activities.
Article
Materials Science, Biomaterials
Kun Liang, Ki Hyun Bae, Akiko Nambu, Bibek Dutta, Joo Eun Chung, Motomi Osato, Motoichi Kurisawa
BIOMATERIALS SCIENCE
(2020)
Review
Materials Science, Biomaterials
Kun Liang, Ki Hyun Bae, Motoichi Kurisawa
JOURNAL OF MATERIALS CHEMISTRY B
(2019)
