Review
Chemistry, Multidisciplinary
Viktoriya Sokolova, Matthias Epple
Summary: Calcium phosphate nanoparticles, due to their similarity to human hard tissue, have high biocompatibility and biodegradability, making them efficient carriers for various biomolecules. They can be functionalized with fluorescing dyes for imaging, and find applications in transfection, gene silencing, imaging, immunization, and bone substitution.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Engineering, Biomedical
Pichaporn Sutthavas, Zeinab Tahmasebi Birgani, Pamela Habibovic, Sabine van Rijt
Summary: Mesoporous silica nanoparticles (MSNs) can effectively promote bone regeneration by surface coating multiple ions, inducing osteogenic gene expression under basic cell culture conditions. Using MSNs as delivery vehicles requires comparatively lower ion doses to achieve similar osteogenic effects compared to direct ion administration in the medium.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Annapaola Coppola, Carmela Vigorito, Patrizia Lombari, Yuselys Garcia Martinez, Margherita Borriello, Francesco Trepiccione, Diego Ingrosso, Alessandra F. Perna
Summary: In this study, the researchers investigated the effect of a new uremic toxin called lanthionine on vascular calcification. The results showed that lanthionine can promote calcification in human endothelial cells by increasing calcium content, inducing the expression of important genes related to mineralization, and affecting the activity of certain proteins. These findings provide insights into the link between uremic toxins and mineral-bone disorder in chronic kidney disease.
Article
Engineering, Biomedical
Nataniel Bialas, Elena K. Muller, Matthias Epple, Ingrid Hilger
Summary: The study showed that cationic silicacoated calcium phosphate nanoparticles carrying encapsulated siRNA can effectively inhibit NF-kappa B p65 gene and protein expression, reduce inflammatory responses, and decrease the expression levels of pro-inflammatory cytokines. These nanoparticles are particularly suitable for the treatment of inflammatory diseases.
Review
Chemistry, Multidisciplinary
Joachim Enax, Frederic Meyer, Erik Schulze zur Wiesche, Matthias Epple
Summary: Calcium and phosphate are essential nutrients for bone and teeth growth, as well as for other biochemical purposes. Calcium phosphates, including hydroxyapatite and tricalcium phosphate, are generally safe as food additives since they dissolve in the stomach and are easily absorbed by the body.
Review
Chemistry, Multidisciplinary
Yupeng Xiao, Zhao Li, Alberto Bianco, Baojin Ma
Summary: This review focuses on the application of calcium ion overload in cancer therapy. Calcium ion overload can induce apoptosis and can be enhanced through different strategies, including surface modification and optimization of nanomaterials, increasing intracellular H+ levels and photothermal effects, and regulating the influx and efflux of calcium ions.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Rashed AlSahafi, Heba Mitwalli, Abdullah Alhussein, Abdulrahman A. Balhaddad, Theeb A. Alquria, Mary Anne S. Melo, Christopher D. Lynch, Thomas W. Oates, Ke Zhang, Hockin. H. K. Xu, Michael D. Weir
Summary: This study developed a novel rechargeable nano-calcium phosphate and nano-calcium fluoride resin-based cement and investigated its mechanical properties and ion release, recharge, and re-release. The results showed that the resin cement with both 12.5% nano-calcium phosphate and 12.5% nano-calcium fluoride demonstrated higher bond strength and flexural strength compared to the commercial control. It also released and re-released calcium, phosphate, and fluoride ions.
JOURNAL OF DENTISTRY
(2022)
Article
Chemistry, Multidisciplinary
Soon Gil Jeong, Yeong Chae Ryu, Byeong Hee Hwang
Summary: The novel synergistic nanocomplexes designed in this study efficiently transfected cells, improved gene expression, and showed good biocompatibility. This new strategy provides an efficient and safe gene carrier for treating various genetic diseases.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Metallurgy & Metallurgical Engineering
Man-fei Yi, Liang-jian Chen, Hui-li He, Lei Shi, Chun-sheng Shao, Bo Zhang
Summary: Improvements in calcium phosphate nanoparticles carriers can enhance gene transfection efficiency and promote osteoblast differentiation. The Mg-CaPNPs-RGD-TAT-CKIP-1 siRNA carrier system showed enhanced cellular uptake and inducement of osteogenesis.
JOURNAL OF CENTRAL SOUTH UNIVERSITY
(2021)
Review
Cell & Tissue Engineering
Mohit Parekh, Vito Romano, Kareem Hassanin, Valeria Testa, Rintra Wongvisavavit, Stefano Ferrari, Atikah Haneef, Colin Willoughby, Diego Ponzin, Vishal Jhanji, Namrata Sharma, Julie Daniels, Stephen B. Kaye, Sajjad Ahmad, Hannah J. Levis
Summary: The corneal endothelium plays a crucial role in maintaining corneal transparency, and its non-regenerative nature necessitates the need for corneal transplants. With limited availability of corneal donor tissues globally, researchers are exploring the cultivation of human corneal endothelial cells in vitro and investigating potential biomaterials for tissue engineering applications.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Engineering, Environmental
Thales R. Machado, Jussara S. da Silva, Renata R. Miranda, Valtencir Zucolotto, Maximo S. Li, M. Valle Martinez de Yuso, Juan J. Guerrero-Gonzalez, Ieda L. V. Rosa, Manuel Algarra, Elson Longo
Summary: In this study, stable activator-free photoluminescent nanoparticles based on amorphous calcium phosphate (ACP) were successfully obtained and their potential application in latent fingerprint imaging was validated. These nanoparticles showed non-toxicity in human skin cells and could produce fingerprint images effectively under near-ultraviolet irradiation.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Zeqing Zhao, Yaxi Sun, Qingchen Qiao, Li Zhang, Xianju Xie, Michael D. Weir, Abraham Schneider, Hockin H. K. Xu, Ning Zhang, Ke Zhang, Yuxing Bai
Summary: The study successfully prevascularized the CPC scaffold by co-culturing hPDLSCs and hUVECs, showing excellent osteogenic and angiogenic capabilities. The co-culture group outperformed monoculture group, presenting promising applications in dental, craniofacial, and orthopedic fields.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Suzana Inkret, Marija Curlin, Kristina Smokrovic, Nikolina Kalcec, Nikolina Peranic, Nadica Maltar-Strmecki, Darija Domazet Jurasin, Maja Dutour Sikiric
Summary: This study investigated the influence of different stabilizing agents on the precipitation of calcium phosphates composites with silver nanoparticles (AgNPs). It was found that AgNPs can modify the precipitation process of calcium phosphates, and the precipitation conditions can be fine-tuned by choosing the appropriate stabilizing agent. This is of special interest for the preparation of biomaterials.
Article
Cell Biology
Kristen L. Segars, Nicholas A. Azzari, Stephanie Gomez, Cody Machen, Celeste B. Rich, Vickery Trinkaus-Randall
Summary: Research has shown that changes in corneal stiffness and signaling contribute to decreased wound healing efficacy in older mice. As mice age, the stiffness of the cornea changes, leading to longer healing times. Additionally, there is reduced calcium signaling at the wound site in older mice, resulting in lower cell activity compared to younger mice.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Physical
Abdullah Alhussein, Rashed Alsahafi, Areej Alfaifi, Mohammad Alenizy, Ibrahim Ba-Armah, Abraham Schneider, Mary-Ann Jabra-Rizk, Radi Masri, Guadalupe Garcia Fay, Thomas W. Oates, Jirun Sun, Michael D. Weir, Hockin H. K. Xu
Summary: This study developed a low-shrinkage-stress nanocomposite with antibiofilm and remineralization capabilities, which effectively countered the progression of recurrent caries. The novel nanocomposite displayed potent antibiofilm effects and released significant amounts of remineralizing ions.
Article
Ophthalmology
Sonia Razafimino, Elias Flockerzi, Elena Zemova, Christian Munteanu, Berthold Seitz
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Editorial Material
Ophthalmology
Doris Fraenkel, Loic Hamon, Isabel Weinstein, Berthold Seitz, Loay Daas
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Ophthalmology
Tim Berger, Berthold Seitz, Loic Hamon, Fidelis Flockerzi, Ursula Schlotzer-Schrehardt, Loay Daas
Summary: This study used light and transmission electron microscopy to examine corneal buttons after DALK surgery. The findings revealed irregularities in collagen distribution and degeneration of keratocytes in the interface area, suggesting that the choice of surgical method can affect the postoperative visual outcome.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Ophthalmology
Haris Sideroudi, Elias Flockerzi, Theresia Jullien, Loic Hamon, Berthold Seitz
Summary: The study investigated the risk factors for keratoconus progression in different age groups. The factors associated with progression were corneal radius, eye rubbing, corneal thinning, contact lens use, and corneal thickness. Further analysis revealed that anterior chamber volume and keratoconus match index were associated with progression in children, while other factors like distance from apex to the thinnest point and Vogt striae were associated with progression in young and middle-aged adults.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Ophthalmology
Kassandra Xanthopoulou, Georgia Milioti, Loay Daas, Cristian Munteanu, Berthold Seitz, Elias Flockerzi
Summary: The efficacy of accelerated epithelium-off corneal crosslinking was evaluated in keratoconus patients < 18 years of age. The study showed that this treatment can stabilize KC progression even > 2 years postoperatively and leads to a deep demarcation line.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Ophthalmology
Zhen Li, Daniel Boehringer, Tanja Stachon, Mahsa Nastaranpour, Fabian Norbert Fries, Berthold Seitz, Myriam Ulrich, Cristian Munteanu, Achim Langenbucher, Nora Szentmary
Summary: The study assessed the impact of various factors, including donor age, storage time, and corneal ring size, on the outgrowth of human limbal epithelial cells. The results showed that lower donor age, fewer medium changes during storage, shorter medium time, and smaller corneoscleral ring size were associated with higher success rates of LEC outgrowth. A stepwise linear regression algorithm was employed to analyze the influencing parameters.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Ophthalmology
Ning Chai, Tanja Stachon, Mahsa Nastaranpour, Zhen Li, Berthold Seitz, Myriam Ulrich, Achim Langenbucher, Nora Szentmary
Summary: This study investigated the effect of Rose Bengal photodynamic therapy (RB-PDT) on the viability and proliferation of different human corneal cells. The results showed that RB-PDT did not have an effect on cell viability or proliferation alone, but it significantly decreased viability and proliferation at specific light doses.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2023)
Article
Ophthalmology
Tim Berger, Nora Szentmary, Ning Chai, Elias Flockerzi, Loay Daas, Tanja Stachon, Berthold Seitz
Summary: The purpose of this research is to investigate the expression of cytokines and ROS-related genes in stromal cells of healthy and keratoconus (KC) corneas. Expression analysis was performed for cytokines and ROS-related genes in corneal fibroblasts (HCFs/KC-HCFs) or keratocytes (Keratocytes/KC-Keratocytes) using qPCR and ELISA. The study found that the expression of most inflammatory markers was decreased in keratocytes compared to fibroblasts, and there were no differences in cytokine expression between healthy and keratoconus cells. TNF-alpha expression was increased in KC keratocytes compared to corneal fibroblasts. No differential expression of ROS-related genes was detected between healthy and diseased cells in both fibroblasts and keratocytes.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2023)
Article
Chemistry, Multidisciplinary
Marina Breisch, Mateusz Olejnik, Kateryna Loza, Oleg Prymak, Nina Rosenkranz, Juergen Buenger, Christina Sengstock, Manfred Koeller, Goetz Westphal, Matthias Epple
Summary: Six types of titanium dioxide particles with different sizes, shapes, and crystal structures were prepared and characterized. The cytotoxicity and induction of reactive oxygen species of these particles were assessed, and it was found that all particles except for rutile microspheres had negligible toxicity. The rough surface of rutile microspheres induced pro-inflammatory cell reactions.
Article
Oncology
Theodora Tsimpaki, Nikolaos E. Bechrakis, Berthold Seitz, Miriam M. Kraemer, Hongtao Liu, Sami Dalbah, Ekaterina Sokolenko, Utta Berchner-Pfannschmidt, Miltiadis Fiorentzis
Summary: The study aimed to establish a new in vivo patient-derived xenograft (PDX) model for uveal melanoma (UM) based on the chick chorioallantoic membrane (CAM) assay. Various techniques and tools were investigated for the assessment of growth, vascularity, and viability of the implanted grafts. The CAM-based PDX model has potential for novel diagnostic and therapeutic experimentation in the field of ocular melanoma.
Article
Chemistry, Multidisciplinary
Kathrin Kostka, Matthias Epple
Summary: Calcium phosphate nanoparticles were modified with polyethyleneimine or carboxymethylcellulose and coated with a silica shell terminated with azide or alkyne groups. These particles were then covalently functionalized with proteins or gold nanoparticles using click chemistry. The functionalized particles showed good dispersibility in water, were easily taken up by cells, and were not cytotoxic.
CHEMISTRY-SWITZERLAND
(2023)
Article
Medicine, General & Internal
Alaa Din Abdin, Machteld Devenijn, Roxana Fulga, Achim Langenbucher, Berthold Seitz, Hakan Kaymak
Summary: This study aimed to investigate the prevalence of geographic atrophy (GA) in advanced age-related macular degeneration (AMD) and the proportion of eyes eligible for treatment with intravitreal pegcetacoplan. A retrospective cross-sectional study was conducted, including all AMD patients who visited a specific center in 2021. It was found that at least a quarter of eyes with advanced AMD would meet the indication criteria for treatment with pegcetacoplan, and neovascular AMD was associated with a higher likelihood of foveal involvement in GA compared to dry AMD.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Health Care Sciences & Services
Madeleine A. Perrault, Gabriele Lauer, Sabine Voss, Berthold Seitz, Barbara Kaesmann-Kellner
Summary: This study aims to compare the etiology and fitting of low vision aids in visually impaired children and adolescents with that of adults. The study found that children had better visual acuity compared to adults and the most common cause of visual impairment in children was albinism while it was age-related macular degeneration in adults. Fitting magnifying visual aids significantly improved near and distance visual acuity, with optical aids being the most commonly prescribed aids.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Ophthalmology
Burak Mergen, Tim Berger, Loic Hamon, Loay Daas, Berthold Seitz
Summary: This case report demonstrates that quadruple DMEK may be a feasible approach for managing chronic endothelial cell decompensation secondary to iris-claw ACIOL implantation.
EUROPEAN JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Ophthalmology
Hakan Kaymak, Kai Neller, Birte Graff, Achim Langenbucher, Berthold Seitz, Hartmut Schwahn, Karsten Klabe
Summary: Patients with mild and moderate macular disorders, such as AMD and ERM, show an induced contrast adaptation, i.e. a gain in contrast sensitivity, which is not different from healthy subjects.
CURRENT EYE RESEARCH
(2023)
