期刊
ACTA BIOMATERIALIA
卷 8, 期 1, 页码 82-88出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2011.08.013
关键词
Cell adhesion; ECM (extracellular matrix); Fibronectin; Micropatterning; Traction force microscopy
资金
- Boston University
- NHLBI [HL-096005]
Quantification of the traction forces that cells apply to their surroundings has been critical to the advancement of our understanding of cancer, development and basic cell biology. This field was made possible through the development of engineered cell culture systems that permit optical measurement of cell-mediated displacements and computational algorithms that allow conversion of these displacements into stresses and forces. Here, we present a novel advancement of traction force microscopy on polyacrylamide (PAA) gels that addresses limitations of existing technologies. Through an indirect patterning technique, we generated PAA gels with fluorescent 1 mu m dot markers in a regularized array. This improves existing traction measurements since (i) multiple fields of view can be measured in one experiment without the need for cell removal; (ii) traction vectors are modeled as discrete point forces, and not as a continuous field, using an extremely simple computational algorithm that we have made available online; and (iii) the pattern transfer technique is amenable to any of the published techniques for producing patterns on glass. In the future, this technique will be used for measuring traction forces on complex patterns with multiple, spatially distinct ligands in systems for applying strain to the substrate, and in sandwich cultures that generate quasi-three-dimensional environments for cells. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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