期刊
ACTA BIOMATERIALIA
卷 8, 期 7, 页码 2795-2806出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2012.04.013
关键词
Osteochondral defect; Amniotic fluid-derived stem cells; Osteogenic differentiation; Chondrogenic differentiation; Bilayered scaffold
资金
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/30745/2006]
- Telemedicine and Advanced Technology Research Center (TATRC) at the US Army Medical Research and Materiel Command [W81XWH-07-1-0718]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/30745/2006] Funding Source: FCT
The development of osteochondral tissue engineered interfaces would be a novel treatment for traumatic injuries and aging associated diseases that affect joints. This study reports the development of a bilayered scaffold, which consists of both bone and cartilage regions. On the other hand, amniotic fluid-derived stem cells (AFSCs) could be differentiated into either osteogenic or chondrogenic cells, respectively. In this study we have developed a bilayered scaffolding system, which includes a starch/polycaprolactone (SPCL) scaffold for osteogenesis and an agarose hydrogel for chondrogenesis. AFSC-seeded scaffolds were cultured for 1 or 2 weeks in an osteochondral-defined culture medium containing both osteogenic and chondrogenic differentiation factors. Additionally, the effect of the presence or absence of insulin-like growth factor-1 (IGF-1) in the culture medium was assessed. Cell viability and phenotypic expression were assessed within the constructs in order to determine the influence of the osteochondral differentiation medium. The results indicated that, after osteogenic differentiation, AFSCs that had been seeded onto SPCL scaffolds did not require osteochondral medium to maintain their phenotype, and they produced a protein-rich, mineralized extracellular matrix (ECM) for up to 2 weeks. However, AFSCs differentiated into chondrocyte-like cells appeared to require osteochondral medium, but not IGF-1, to synthesize ECM proteins and maintain the chondrogenic phenotype. Thus, although IGF-1 was not essential for creating osteochondral constructs with AFSCs in this study, the osteochondral supplements used appear to be important to generate cartilage in long-term tissue engineering approaches for osteochondral interfaces. In addition, constructs generated from agarose-SPCL bilayered scaffolds containing pre-differentiated AFSCs may be useful for potential applications in regeneration strategies for damaged or diseased joints. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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