Article
Biochemistry & Molecular Biology
Megane Beldjilali-Labro, Rachid Jellali, Alexander David Brown, Alejandro Garcia Garcia, Augustin Lerebours, Erwann Guenin, Fahmi Bedoui, Murielle Dufresne, Claire Stewart, Jean-Francois Grosset, Cecile Legallais
Summary: The goal of this study was to design and evaluate a multi-scale scaffold for promoting the differentiation of C2C12 skeletal myoblasts. The researchers found that the scaffold had tunable mechanical properties and improved the alignment and length of myotubes, leading to enhanced organization of reconstructed skeletal muscle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Abolfazl Mozaffari, Mazeyar Parvinzadeh Gashti
Summary: In this study, tannic acid-crosslinked gelatin nanofibers were fabricated by electrospinning and treated with atmospheric air plasma to enhance their bio-functionality. The results showed that atmospheric air plasma treatment not only improved the hydrophilicity of the nanofibers but also enhanced their bio-functionality against human skin fibroblast cells. Therefore, atmospheric air plasma pre-treatment is recommended for the surface functionalization of gelatin nanofibers in skin tissue engineering applications.
Article
Biotechnology & Applied Microbiology
Lisanne Terrie, Margherita Burattini, Sandra Van Vlierberghe, Lorenzo Fassina, Lieven Thorrez
Summary: Skeletal muscle tissue engineering aims to generate 3D engineered constructs that mimic the structure and function of native muscle. Pulsed electromagnetic field stimulation enhances myotube formation in both 2D and 3D cultures, increasing myotube diameter, fusion index, and myosin heavy chain expression.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Abolfazl Mozaffari, Mazeyar Parvinzadeh Gashti, Mohammad Mirjalili, Masoud Parsania
Summary: The study demonstrated that functionalization of gelatin nanofibers using plasma method can improve their biocompatibility. After plasma treatment, the surface morphology and diameter of nanofibers remained unchanged, but the surface roughness increased and the nanofibers became completely hydrophilic. Using plasma-treated gelatin nanofibers can increase the number of fibroblast cells.
Review
Polymer Science
Muhammad Zikri Aiman Zulkifli, Darman Nordin, Norazuwana Shaari, Siti Kartom Kamarudin
Summary: Tissue engineering integrates biology, medicine, and engineering to design biological substitutes for tissue functions, aiming to avoid organ transplantation. Electrospinning, a widely used technique, can fabricate nanofibrous scaffolds with high surface-to-volume ratio and the ability to mimic extracellular matrices, which are desirable for tissue engineering. However, electrospun scaffolds have limitations such as poor cell penetration and load-bearing capabilities. Various research groups have proposed solutions to overcome these limitations.
Article
Biotechnology & Applied Microbiology
Ana Elisa Antunes dos Santos, Tiago Cotta, Joao Paulo Ferreira Santos, Juliana Sofia Fonseca Camargos, Ana Carolina Correia do Carmo, Erika Gabriele Alves Alcantara, Claudia Fleck, Aline Goncalves Lio Copola, Julia Meireles Nogueira, Gerluza Aparecida Borges Silva, Luciana de Oliveira Andrade, Roberta Viana Ferreira, Erika Cristina Jorge
Summary: Electrospinning is a promising technique for scaffolds production in cultivated meat due to its simplicity, versatility, cost-effectiveness, and scalability. Cellulose acetate (CA) is a biocompatible and low-cost material that can support cell adhesion and proliferation. This study investigated CA nanofibers, with or without a bioactive annatto extract (CA@A), as potential scaffolds for cultivated meat and muscle tissue engineering. The results showed that CA@A nanofibers had increased fiber diameter and reduced stiffness compared to pure CA nanofibers, indicating their potential application as a scaffold for long-term muscle cells culture.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Suihong Liu, Liguo Sun, Haiguang Zhang, Qingxi Hu, Yahao Wang, Murugan Ramalingam
Summary: This study aims to design and fabricate mechanically-tunable nerve conduits with biomimetic structural features of the human nerve, and the results confirm the potential of the combinatorial approach in fabricating mechanically-tunable triple-layered conduits.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agriculture, Multidisciplinary
Qi Zheng, Rong-cui Hu, Cui-yun Zhu, Jing Jing, Meng-yu Lou, Si-huan Zhang, Shuang Li, Hong-guo Cao, Xiao-rong Zhang, Ying-hui Ling
Summary: This study characterized the changes in proteomic and transcriptomic expression profiles during the transition from skeletal muscle satellite cells (SSCs) to myotube formation. Potential molecular mechanisms and signaling pathways involved in this transition were identified, and the roles of specific proteins such as SLMAP, STOM, SVIL, AQP1, and SERPINE1 were highlighted. These findings enhance our understanding of myotube formation during early development and repair.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Biotechnology & Applied Microbiology
Michela Licciardello, Gianluca Ciardelli, Chiara Tonda-Turo
Summary: In this study, nanofibers based on a PCL-PANI blend were successfully fabricated using electrospinning technology. The resulting fibrous substrate showed good stability in physiological conditions. To improve the hydrophilicity of the surface, atmospheric plasma treatment was applied, resulting in a more favorable biological response. In vitro tests confirmed the viability and physiological-like morphology of cells cultured on the plasma treated PCL-PANI nanofibrous scaffolds.
BIOENGINEERING-BASEL
(2021)
Review
Biotechnology & Applied Microbiology
Britani N. Blackstone, Summer C. Gallentine, Heather M. Powell
Summary: This systematic review examines the use of electrospun collagen in tissue engineering applications, finding that different crosslinking methods can affect degradation resistance and strength of the scaffolds, which are capable of forming engineered tissues with high similarity to native structures.
BIOENGINEERING-BASEL
(2021)
Article
Materials Science, Multidisciplinary
David Sonnleitner, Natascha Schaefer, Annalena Wieland, Lena Fischer, Patrick Pasberg, Ingo Thievessen, Gregor Lang
Summary: This study presents a completely novel morphology of dumbbell-shaped PCL micro-particles, which combines structural features of fibers and spheres and has great potential in medical applications. The complex structures can be achieved by balancing parameters and spinning dope compositions, allowing for precise control over the aspect ratio of the particles and significantly enhanced cell adhesion.
APPLIED MATERIALS TODAY
(2021)
Review
Biotechnology & Applied Microbiology
Fei Xu, Chloe Dawson, Makenzie Lamb, Eva Mueller, Evan Stefanek, Mohsen Akbari, Todd Hoare
Summary: This review addresses the key design criteria and emerging fabrication techniques for translationally-relevant tissue engineering hydrogels. It suggests combining integrated fabrication approaches with successful translational experiences to accelerate the implementation of hydrogels in tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Ahmad Saudi, Seyed Mojtaba Zebarjad, Hossein Salehi, Elham Katoueizadeh, Aliakbar Alizadeh
Summary: The study examined the morphology, surface contact angle, mechanical properties, in vitro degradation behavior, and water uptake of PCL/PGS/HAp fibers, showing that HAp particles have a positive effect on PC12 viability and adhesion.
MATERIALS CHEMISTRY AND PHYSICS
(2022)
Review
Materials Science, Multidisciplinary
Miaomiao Zhang, Shixin Xu, Ruoying Wang, Yongan Che, Cuicui Han, Wei Feng, Chengwei Wang, Wen Zhao
Summary: Electrospun nanofiber/hydrogel composites have gained significant attention due to their combination of excellent biochemical properties of hydrogel with the biomimetic nature of electrospun fibers. These composites, with tunable mechanical properties, can mimic the microstructure of extracellular matrix (ECM) and microenvironment of cells, making them advantageous for tissue repair. However, a comprehensive review on their application as tissue engineering scaffolds is lacking. This article systematically reviews the preparation and application of electrospun fiber/hydrogel composites in tissue engineering, providing insights for future research directions.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Muheeb Rafiq, Sami-ullah Rather, Taha Umair Wani, Anjum Hamid Rather, Rumysa Saleem Khan, Anees Ellahi Khan, Ibtisam Hamid, Haseeb A. Khan, Abdullah S. Alhomida, Faheem A. Sheikh
Summary: After the discovery of MXene, a new class of 2D material, researchers have found a new scope for their research. MXenes have remarkable physical, chemical, and biological properties, making them useful in various research disciplines. They have been applied in biosensors, bioimaging, tissue engineering, drug delivery systems, and more. MXenes can be functionalized with different atoms/molecules, making them diverse materials. Their potential in nanofibers is even greater than expected.
CHINESE CHEMICAL LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Sandra Gaiser, Urs Schutz, Patrick Rupper, Dirk Hegemann
Article
Cell Biology
Tatiana A. Kovalenko, Marie-Noelle Giraud, Anita Eckly, Anne-Sophie Ribba, Fabienne Proamer, Sandrine Fraboulet, Nadezhda A. Podoplelova, Jeremy Valentin, Mikhail A. Panteleev, Carmen Gonelle-Gispert, Stephane Cook, Laurence Lafanechere, Anastasia N. Sveshnikova, Karin Sadoul
Summary: Primary hemostasis involves platelet activation, while secondary hemostasis generates a fibrin clot where active platelets contribute to clot retraction and change its structure over time. Research suggests that platelet distribution within the clot influences clot architecture, with computational models predicting asymmetric forces leading to different platelet contractile behaviors in the periphery versus the center of the clot.
Article
Materials Science, Multidisciplinary
Anne Geraldine Guex, David J. Poxson, Daniel T. Simon, Magnus Berggren, Giuseppino Fortunato, Rene M. Rossi, Katharina Maniura-Weber, Markus Rottmar
Summary: This study proposes a method to manipulate fibroblast to myofibroblast differentiation by controlling the pH of the cellular microenvironment using electronic ion pump technology, leading to reduced expression of scar-related markers and promoting scar-free wound healing.
APPLIED MATERIALS TODAY
(2021)
Article
Chemistry, Multidisciplinary
Jean Schoeller, Fabian Itel, Karin Wuertz-Kozak, Sandra Gaiser, Nicolas Luisier, Dirk Hegemann, Stephen J. Ferguson, Giuseppino Fortunato, Rene M. Rossi
Summary: Surface functionalization of electrospun nanofibers allows for the introduction of additional functionalities while retaining high porosity and surface-to-volume ratio. In this study, chitosan and alginate were sequentially deposited on PLGA nanofibers to form a polyelectrolyte complex for controlled release of ibuprofen. Chitosan with a positive surface charge regulated the interactions between the drug and the coating, resulting in slower release at acidic pH compared to neutral pH.
Article
Biochemistry & Molecular Biology
Ines Borrego, Aurelien Frobert, Guillaume Ajalbert, Jeremy Valentin, Cyrielle Kaltenrieder, Benoit Fellay, Michael Stumpe, Stephane Cook, Joern Dengjel, Marie-Noelle Giraud
Summary: This study found that bone marrow/fibrin-based treatment improved cardiac structure and function after myocardial infarction. The results showed that fibrin influenced gene expression and secretome of bone marrow cells, leading to increased spreading of cardiac cells and proliferation of anti-inflammatory macrophages, which sequentially stimulated cardiac cell proliferation.
Article
Cell Biology
Alexandre Balaphas, Jeremy Meyer, Cecile Gameiro, Aurelien Frobert, Marie-Noelle Giraud, Bernhard Egger, Leo H. Buhler, Carmen Gonelle-Gispert
Summary: The aim of this study was to optimize the isolation of hepatic stellate cells (HSCs) from mouse livers and obtain highly pure HSC populations. Through a two-step collagenase digestion and Nycodenz gradient separation followed by CD11b-negative sorting, we successfully isolated HSCs with high yield, viability, and purity. Flow cytometry analysis showed that CD38 may be a reliable marker for HSCs.
Article
Chemistry, Medicinal
Manish Jain, Jordan Bouilloux, Ines Borrego, Stephane Cook, Hubert van den Bergh, Norbert Lange, Georges Wagnieres, Marie-Noelle Giraud
Summary: Cathepsin B plays an important role in cancer, atherosclerosis, and other inflammatory diseases. A cathepsin B-cleavable polymeric photosensitizer prodrug was synthesized for photodynamic therapy (PDT) of cancer, which showed potential for selective destruction of cells with high cathepsin B activity.
Article
Materials Science, Multidisciplinary
Kamran Iranshahi, Jean Schoeller, Nicolas Luisier, Michael Chung, Sina Hashemizadeh, Giuseppino Fortunato, Thijs Defraeye, Rene M. Rossi
Summary: This paper explores the impact of solution properties on improving the electrospinning throughput through experimental and theoretical research. It is found that adding polysiloxane additives can significantly improve the production rate of nanofibrous scaffolds by reducing solution surface tension and increasing solution viscosity. Adjusting solution properties is a key factor in increasing electrospinning throughput.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Physiology
Martin Flueck, David Vaughan, Joern Rittweger, Marie-Noelle Giraud
Summary: In this study, the researchers found that carriers of the ACE-DD genotype have an increased risk of developing inactivity-related type II diabetes and exhibit higher blood glucose concentration during exercise compared to carriers of the insertion allele. They observed differences in the activity levels of glucose uptake and metabolism-related factors, as well as AKT phosphorylation levels, in muscle biopsies and blood samples of ACE-DD genotype carriers after exercise.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Chemistry, Multidisciplinary
Lea A. Furer, Angela Diaz Abad, Pius Manser, Yvette Hannig, Simone Schuerle, Giuseppino Fortunato, Tina Buerki-Thurnherr
Summary: The design of safe and effective nanoparticles for commercial and medical applications requires a thorough understanding of their translocation and effects in biological barriers. In this study, thin chitosan/polyethylene oxide membranes with a high porosity and nanofibrous morphology were developed using electrospinning, allowing for the cultivation of a functional placental monolayer. The transfer studies using these membranes showed significantly enhanced permeability compared to commercial microporous membranes, and the transfer data of nanoparticles was highly similar to ex vivo perfusion studies of intact human placental tissue. These newly developed membranes could contribute to establishing physiologically relevant in vitro biobarrier transfer models with superior permeability for a wide range of molecules and particles.
Article
Chemistry, Multidisciplinary
Mohammad-Reza Norouzi, Laleh Ghasemi-Mobarakeh, Fabian Itel, Jean Schoeller, Hossein Fashandi, Aurelio Borzi, Antonia Neels, Giuseppino Fortunato, Rene M. Rossi
Summary: In this study, sodium alginate/poly(epsilon-caprolactone) core/shell nanofibers were successfully fabricated via water-in-oil emulsion electrospinning. The optimal emulsion conditions were determined, and the nanofibers were characterized using various techniques. The results demonstrated the favorable biocompatibility of the nanofibers, making them a promising material for biomedical applications.
NANOSCALE ADVANCES
(2022)
Article
Materials Science, Multidisciplinary
Efe Armagan, Kongchang Wei, Giuseppino Fortunato, Esther Amstad, Rene Michel Rossi
Summary: The purely organic optical O-2 sensor developed in this study utilizes polyimide-based photoemitters embedded within a poly(vinyl alcohol) matrix for continuous and broad-range O-2 sensing in the range of 0-16% O-2. The synthesized photoemitters demonstrate higher sensitivity and broader sensing range to O-2 with longer reaction times, leading to a more rigid polyimide structure. The sensor exhibits a Stern-Volmer quenching constant of 0.2351 kPa(-1) for the linear response range of 0-4% O-2 and can be repetitively used at least 10 times in both the linear range and beyond.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Adrienne Muller, Claudia Fessele, Flavia Zuber, Markus Rottmar, Katharina Maniura-Weber, Qun Ren, Anne Geraldine Guex
Summary: This study developed electrospun membranes of poly(4-hydroxybutyrate) functionalized with gallium protoporphyrin, which showed antibacterial effects against Staphylococcus aureus. The membranes did not exhibit cytotoxic effects on human dermal fibroblasts in vitro, indicating their potential as alternative antibacterial strategies for skin wound treatment.
ACS APPLIED BIO MATERIALS
(2021)