Article
Chemistry, Applied
Duc Dung Nguyen, Chun-Hsu Yao, Li-Jyuan Luo, Hung-Chi Chen, Yi-Jen Hsueh, David Hui-Kang Ma, Jui-Yang Lai
Summary: This study explores the effects of oxidation level on gelatin microcarriers for repairing corneal injuries, and finds that high oxidation levels can enhance the adhesion and growth of keratocytes. In a rabbit model of corneal alkali burn injury, the use of microcarriers with appropriate oxidation level effectively reduces corneal swelling, promotes collagen production and keratocan expression, and repairs the collagenous stromal architecture.
CARBOHYDRATE POLYMERS
(2022)
Review
Biochemistry & Molecular Biology
Mithun Santra, Yu-Chi Liu, Vishal Jhanji, Gary Hin-Fai Yam
Summary: Corneal transparency is crucial for vision, but corneal opacity can cause blindness. Conventional corneal transplantation is limited by a lack of donor tissue, making bioengineered corneal tissues an emerging solution. Engineering with extracellular matrix (ECM) scaffolds, particularly using ultra-thin stromal laminar tissues obtained from refractive correction procedures, offers a promising approach for corneal regeneration and restoration of transparency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Adrien Fayon, Deborah Helle, Gregory Francius, Jean-Baptiste Vincourt, Veronique Regnault, Dominique Dumas, Patrick Menu, Reine El Omar
Summary: This study presents an innovative method to extract human ECM derived from the Wharton's jelly of umbilical cords, which shows promising applications in tissue engineering like direct use in vascular tissue engineering. The efficient decellularization and characterization of WJ-ECMaa suggest it can be used in allogeneic conditions with better cytocompatibility and hemocompatibility compared to conventional coatings.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Stefania Croce, Lorenzo Cobianchi, Tamara Zoro, Francesca Dal Mas, Antonia Icaro Cornaglia, Elisa Lenta, Gloria Acquafredda, Annalisa De Silvestri, Maria Antonietta Avanzini, Livia Visai, Szandra Brambilla, Giovanna Bruni, Giulia Di Gravina, Andrea Pietrabissa, Luca Ansaloni, Andrea Peloso
Summary: This study investigates the effect of extracellular matrix (ECM) on the differentiation of porcine mesenchymal stromal cells (pMSCs) into hepatocyte-like cells (HLCs) through decellularization and reseeding. The results suggest that ECM may promote the differentiation of pMSCs into HLCs, but contact with liver ECM alone is not sufficient for complete differentiation.
Article
Biotechnology & Applied Microbiology
Yili Wang, Feng Wen, Xueting Yao, Lulu Zeng, Jiaming Wu, Qinhong He, Huaqiong Li, Lian Fang
Summary: A novel series of composite hydrogels, composed of HAMA, GelMA, and ECM, were prepared and characterized for potential utility in repairing damaged tympanic membrane. The absence of alpha-1,3-galactosyl glycoprotein in the ECM component from alpha-1,3 GalT-KO pigs could prevent rejection. The results are promising but further development is needed for surgical application.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Engineering, Biomedical
Derek W. Nelson, Ryan J. Gilbert
Summary: ECM-mimetic hydrogels are promising regenerative scaffolds for injured nervous system, providing 3D bioactive interfaces to modulate cellular response and support tissue remodeling. Future advancements in their design and use may lead to viable treatments for patients with neural injury.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Promita Bhattacharjee, Mark Ahearne
Summary: There is a need to develop tissue engineering methods to address the shortage of donor corneas for transplantation. A novel approach using free-radical polymerization to fabricate three-dimensional hydrogels for corneal stromal tissue regeneration was investigated. The hydrogels showed rapid gelation, good porosity, enhanced cellular adhesion and proliferation, and no cytotoxicity. This study provides an initial step towards developing clinically applicable ocular implants.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Materials Science, Biomaterials
Swatilekha Hazra, Souradeep Dey, Biman B. Mandal, Charanya Ramachandran
Summary: Silk films have potential as substrates for culturing human corneal endothelial cells. Despite their higher tensile strength compared to native basement membrane, the expression of extracellular matrix proteins and integrins on silk films is comparable to native tissue, confirming their suitability for engineering corneal endothelial cells for transplantation.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Engineering, Biomedical
Cindy J. Farino Reyes, Shantanu Pradhan, John H. Slater
Summary: The study investigates the role of hydrogel properties in regulating the phenotype of triple negative metastatic breast cancer using different cell lines and hydrogel formulations. The results show that hydrogels with varying adhesivity and degradability have different effects on cell growth, dormancy, and other metrics. These findings demonstrate that ECM properties and organ-tropism synergistically regulate cancer cell phenotype and dormancy.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Polymer Science
Bryan Falcones, Hector Sanz-Fraile, Esther Marhuenda, Irene Mendizabal, Ignacio Cabrera-Aguilera, Nanthilde Malandain, Juan J. Uriarte, Isaac Almendros, Daniel Navajas, Daniel J. Weiss, Ramon Farre, Jorge Otero
Summary: Mesenchymal stromal cell (MSC) therapy for acute respiratory diseases relies on MSCs' secretion of paracrine factors. Preconditioning the MSCs by growing them in a lung-derived extracellular matrix (ECM) can enhance their therapeutic efficacy. Bioink based on decellularized porcine lung ECM hydrogels supports 3D culturing of lung-resident MSCs without additional crosslinking, leading to improved cell-matrix interactions and increased expression of key receptors. This approach shows promise for enhancing MSC preconditioning for therapeutic applications.
Article
Engineering, Biomedical
Pujhitha Ramesh, Nicholas Moskwa, Zachary Hanchon, Adam Koplas, Deirdre A. Nelson, Kristen L. Mills, James Castracane, Melinda Larsen, Susan T. Sharfstein, Yubing Xie
Summary: In this study, cryoelectrospinning was used to fabricate scaffolds that mimic the structure and function of soft-tissue ECM. The researchers found that these scaffolds supported cell survival and exhibited potential for use in regenerative medicine applications.
Article
Cell Biology
Tim Hammersen, Justyna Buchert, Severin Zietzschmann, Solvig Diederichs, Wiltrud Richter
Summary: Increasing extracellular calcium levels can enhance cartilage neogenesis from human articular chondrocytes and bone marrow-derived mesenchymal stromal cells, but the effect is opposite for the two cell types.
Article
Cell Biology
Ana C. Acosta, Mei Sun, Nabeel Zafrullah, Marcel Y. Avila, Curtis E. Margo, Edgar M. Espana
Summary: During corneal wound healing, activated fibroblasts and myofibroblasts attempt to restore tissue integrity and function. This study found that these transformed cells can regain their normal phenotype once relocated to an ECM with normal signaling.
DISEASE MODELS & MECHANISMS
(2023)
Article
Engineering, Biomedical
Shengran Wang, Changlai Zhu, Bin Zhang, Junxia Hu, Jinghui Xu, Chengbin Xue, Shuangxi Bao, Xiaokun Gu, Fei Ding, Yumin Yang, Xiaosong Gu, Yun Gu
Summary: A favorable microenvironment is crucial for nerve regeneration. This study investigates the components and spatial organization of extracellular matrix (ECM) secreted by different cells and finds that ECM derived from bone marrow mesenchymal cells (BMSCs) is more similar to acellular nerves. ECM-modified nerve grafts (ECM-NGs) co-cultivated with BMSCs exhibit the most promising nerve repair properties and create a superior microenvironment for nerve regeneration, indicating a potential alternative for clinical repair of peripheral nerve defects.
Article
Engineering, Biomedical
Manuela Lopera Higuita, Juan F. Lopera Giraldo, Tiffany L. Sarrafian, Leigh G. Griffiths
Summary: Diseases of small diameter blood vessels are a major part of cardiovascular diseases, with many patients ineligible for autologous vascular grafting. Non-antigenic ECM scaffolds from xenogeneic vascular tissue show potential as ideal off-the-shelf vascular grafts for treating small diameter vascular diseases.
ACTA BIOMATERIALIA
(2021)
Article
Chemistry, Multidisciplinary
Li-Jyuan Luo, Duc Dung Nguyen, Jui-Yang Lai
JOURNAL OF CONTROLLED RELEASE
(2020)
Article
Materials Science, Biomaterials
Duc Dung Nguyen, Li-Jyuan Luo, Jui-Yang Lai
Summary: Understanding the complex interaction between therapeutic nanoparticles and biological entities is crucial for developing effective disease treatments. In this study, different geometrical impacts of silver nanoparticles on their biological activities towards Staphylococcus aureus-induced keratitis were thoroughly assessed. The findings suggest that the geometric effects of therapeutic nanoparticles play a critical role in preclinical treatment of eye-related microbial infections.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Engineering, Environmental
Hung-Yun Lin, Sin-Wen Wang, Ju-Yi Mao, Huan-Tsung Chang, Scott G. Harroun, Han-Jia Lin, Chih-Ching Huang, Jui-Yang Lai
Summary: In this study, carbonized nanogels synthesized from quercetin and lysine were developed to have antibacterial and antioxidant properties for the treatment of bacterial keratitis. The nanogels showed broad-spectrum bacteriostatic effects against various bacteria and good biocompatibility in vitro and in vivo, making them a potential effective treatment for bacterial ocular diseases.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biophysics
Duc Dung Nguyen, Shingjiang Jessie Lue, Jui-Yang Lai
Summary: The size of silver nanoparticles plays a crucial role in their therapeutic efficacy in treating Staphylococcus aureus keratitis, with medium-sized particles demonstrating the best treatment outcomes.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Materials Science, Multidisciplinary
Li-Jyuan Luo, Hong-Jyuan Jian, Scott G. Harroun, Jui-Yang Lai, Binesh Unnikrishnan, Chih-Ching Huang
Summary: The study demonstrated a potential nanocomposite for treating AMD through sustained drug release and targeted therapy. The findings suggest a great potential for clinical intervention in AMD treatment with the multifunctional nanocomposite.
APPLIED MATERIALS TODAY
(2021)
Article
Engineering, Biomedical
Pei-Hsuan Lin, Hong-Jyuan Jian, Yu-Jia Li, Yu-Fen Huang, Anisha Anand, Chih-Ching Huang, Han-Jia Lin, Jui-Yang Lai
Summary: In this study, carbonized nanogels (CNGs) were developed as long-lasting eye drops for the treatment of dry eye syndrome (DES). These CNGs show high biocompatibility and anti-inflammatory properties, providing significant therapeutic effects and holding great potential as innovative drugs for the treatment of dry eye disease.
ACTA BIOMATERIALIA
(2022)
Article
Medicine, Research & Experimental
Yi-Jen Hsueh, Yaa-Jyuhn James Meir, Jui-Yang Lai, Chieh-Cheng Huang, Tsai-Te Lu, David Hui-Kang Ma, Chao-Min Cheng, Wei-Chi Wu, Hung-Chi Chen
Summary: In this study, topical application of ascorbic acid (AA) was found to ameliorate benzalkonium chloride-induced rabbit corneal endothelial damage. The mechanism involved the transportation of AA into B4G12 cells via GLUT1, leading to increased ERK phosphorylation and cell proliferation. These findings suggest that CEC proliferation stimulated through the noncanonical AA-GLUT1-ERK axis contributes to AA-enhanced healing of corneal endothelial dysfunction (CED).
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Engineering, Environmental
Li-Jyuan Luo, Duc Dung Nguyen, Chih-Ching Huang, Jui-Yang Lai
Summary: This study reports an advanced therapeutic hydrogel sheet constructed using a functional hydrogel and a ternary drug-carrier system, providing continuous multistage drug release for the treatment of corneal abrasion with significant efficacy, representing over an eight-fold improvement compared to conventional eye drops.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Cell & Tissue Engineering
David Hui-Kang Ma, Yi-Jen Hsueh, Kevin Sheng-Kai Ma, Yueh-Ju Tsai, Shiang-Fu Huang, Hung-Chi Chen, Chi-Chin Sun, Ming-Tse Kuo, An-Shine Chao, Jui-Yang Lai
Summary: The study presented long-term outcomes of two clinical cases of COMET transplantation, demonstrating the promising potential of collagenase-based suspension culture technique for enhancing the long-term survival of transplanted OMECs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Engineering, Biomedical
Duc Dung Nguyen, Li-Jyuan Luo, Jui-Yang Lai
Summary: This study reports a rational strategy to develop a therapeutic thermogel with prolonged anti-inflammatory and corneal-protective effects, showing great promise for topical treatment of ocular inflammation and advancement of ophthalmic formulations.
MATERIALS TODAY BIO
(2022)
Review
Engineering, Environmental
Duc Dung Nguyen, Jui-Yang Lai
Summary: ITNPs are a special class of nanomaterials with intrinsic therapeutic capabilities, which can be utilized to treat diseases associated with oxidative stress, inflammation, angiogenesis, and infection. Recent advancements in the field of nanotherapeutics have been significantly influenced by ITNPs.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Yu-Jia Li, Shih-Chun Wei, Han-Wei Chu, Hong-Jyuan Jian, Anisha Anand, Amit Nain, Yu-Fen Huang, Huan-Tsung Chang, Chih-Ching Huang, Jui-Yang Lai
Summary: In this study, a novel nanoVelcro dressing made from quercetin (QNV) was developed, which exhibited antibacterial, anti-oxidative, and anti-inflammatory effects, as well as endotoxin neutralization. The dressing effectively inhibited the growth of various bacteria and accelerated wound closure rate in infected wounds.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Anisha Anand, Binesh Unnikrishnan, Ju-Yi Mao, Chin-Jung Lin, Jui-Yang Lai, Chih-Ching Huang
Summary: This study demonstrates the use of CNGs/GO nanocomposite membrane for the efficient removal of Gram-negative and Gram-positive bacteria from contaminated water. The membrane shows great potential as a filter without any extra power consumption.
Article
Medicine, Research & Experimental
Li-Jyuan Luo, Duc Dung Nguyen, Jui-Yang Lai
Summary: By controlling the shell thickness of hollow mesoporous ceria nanoparticles (HMCNs), sustained release of Y-27632 was achieved, demonstrating effective treatment for up to 10 days in a rabbit model of glaucoma.
Article
Materials Science, Biomaterials
Han-Wei Li, Ju-Yi Mao, Chia-Wen Lien, Chu-Kuei Wang, Jui-Yang Lai, Ranju Prasad Mandal, Huan-Tsung Chang, Lung Chang, David Hui-Kang, Chih-Ching Huang
JOURNAL OF MATERIALS CHEMISTRY B
(2020)