Article
Clinical Neurology
Qiao Zhang, Jinlin Chen, Jingjing Lin, Ruichao Liang, Min He, Yanchao Wang, Hong Tan
Summary: In this study, a three-dimensional biodegradable waterborne polyurethane scaffold was investigated as a tool to promote endogenous brain tissue regeneration after intracerebral hemorrhage (ICH). The results showed that the scaffold could attract immune cells and modulate their types and secretions to promote endogenous brain regeneration.
TRANSLATIONAL STROKE RESEARCH
(2023)
Article
Engineering, Biomedical
Qilong Zhao, Yu Zhou, Min Wang
Summary: This study demonstrates a new method for directly placing living endothelial cells within bioactive nanofibrous scaffolds in 3D, using concurrent emulsion electrospinning and coaxial cell electrospraying. The technique allows for deep cell distribution and preservation of cell viability, leading to promising bioactive nanofibrous scaffolds with 3D cell incorporation. By combining structural and biochemical cues, the 3D cell-incorporated scaffolds offer an innovative approach to tissue engineering for creating vascularized structures.
ACTA BIOMATERIALIA
(2021)
Article
Materials Science, Multidisciplinary
Yuxiao Zhou, Warren Grayson
Summary: This article summarizes the application of 3D printing technology in craniofacial bone reconstruction. Through layer-by-layer manufacturing techniques, patient-specific craniofacial structures can be produced for esthetic purposes and functional restoration. The article also discusses the remaining hurdles and opportunities for growth in this technology.
Review
Materials Science, Multidisciplinary
Yujie Chen, Xutao Dong, Muhammad Shafiq, Gregory Myles, Norbert Radacsi, Xiumei Mo
Summary: Electrospinning is a technique used to fabricate nanofibrous three-dimensional scaffolds for tissue engineering, mimicking the extracellular matrix. The selection of materials, methods, and post-processing techniques significantly affect the structure, function, and applications of the scaffolds.
ADVANCED FIBER MATERIALS
(2022)
Review
Polymer Science
Xiangjun Yang, Yuting Wang, Ying Zhou, Junyu Chen, Qianbing Wan
Summary: PCL is widely used in bone tissue engineering for its good biocompatibility and slow degradation rate, and can be blended with other polymers and hydrogels to improve its properties. 3D printing techniques are utilized to fabricate PCL-based composite scaffolds for various clinical applications.
Article
Biophysics
Claudia A. Silva, Margarida M. Fernandes, Clarisse Ribeiro, Senentxu Lanceros-Mendez
Summary: The incidence of bone disorders is increasing, calling for new strategies for bone repair. This study presents the development of piezoelectric scaffolds that mimic the bone's structure and mechano-electric microenvironment to promote cell proliferation and adhesion. The results show that these electromechanically-responsive scaffolds can significantly enhance the proliferation rate and adhesion of osteoblastic cells.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Review
Engineering, Biomedical
Lucas Fabricio Bahia Nogueira, Bianca C. Maniglia, Rene Buchet, Jose Luis Millan, Pietro Ciancaglini, Massimo Bottini, Ana Paula Ramos
Summary: Bones are both an organ and a material, consisting of a hybrid organic/inorganic matrix formed and regulated by a complex array of cells. Understanding the structure and functions of bones can help in developing scaffolds for bone tissue engineering.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Review
Engineering, Biomedical
Shi Hua Tan, Dun An Cliff Chua, Je Re Jeremiah Tang, Carine Bonnard, David Leavesley, Kun Liang
Summary: In vitro 3D human skin models are critical tools in advancing our understanding of skin physiology and function. Many of the existing reconstructed models are limited in terms of structure and complexity, thus failing to recapitulate native human skin. Hydrogels have been identified as useful scaffold materials for fabricating the dermal equivalent of 3D skin models, allowing for greater flexibility and control in scaffold properties and cellular incorporation. This review aims to provide a critical discussion of the biomaterial selection and design strategies in the construction of hydrogel-based full-thickness skin equivalents. At the same time, insights into future developments and technological advances which can accelerate progress in this field are offered.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Greta Babakhanova, Anant Agrawal, Deepika Arora, Allison Horenberg, Jagat B. Budhathoki, Joy P. Dunkers, Joe Chalfoun, Peter Bajcsy, Carl G. Simon
Summary: In tissue engineering, the combination of 3D scaffolds and cells is used to repair or restore tissue function. This study developed a noninvasive OCT method to rapidly assess cell viability and distribution within 3D scaffolds. The results demonstrate the importance of this method for evaluating tissue-engineered medical products.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Engineering, Biomedical
Ziqi Gan, Yifan Zhao, Yeke Wu, Wei Yang, Zhihe Zhao, Lixing Zhao
Summary: Temporomandibular disorder (TMD) is a clinical challenge with limited therapeutic methods. We developed a biomimetic scaffold with optimal reinforcement, demonstrating its superior properties and potential for complex tissue regeneration in animal models.
ACTA BIOMATERIALIA
(2022)
Article
Cell & Tissue Engineering
Prasad Sawadkar, Nandin Mandakhbayar, Kapil D. Patel, Jennifer Olmas Buitrago, Tae Hyun Kim, Poojitha Rajasekar, Ferdinand Lali, Christos Kyriakidis, Benyamin Rahmani, Jeviya Mohanakrishnan, Rishbha Dua, Karin Greco, Jung-Hwan Lee, Hae-Won Kim, Jonathan Knowles, Elena Garcia-Gareta
Summary: The study focuses on using natural polymer scaffolds seeded with stem cells to improve soft tissue repair, finding that collagen-based materials exhibit good cell adhesion and proliferation in vitro, with superior adipogenic properties in vivo. In contrast, fibrin shows poor cellular and adipogenesis properties but higher angiogenesis. Elastin forms the most porous scaffold, with non-aggregated cell morphology in vitro, and degrades the fastest in vivo.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
R. Olmos-Juste, G. Larranaga-Jaurrieta, I. Larraza, S. Ramos-Diez, S. Camarero-Espinosa, N. Gabilondo, A. Eceiza
Summary: In this study, functional alginate and waterborne polyurethane scaffolds were developed for articular cartilage regeneration using 3D bioprinting technology. It was found that scaffolds with a higher alginate content showed better 3D printing performance, structural integrity, and cell viability. After 28 days of experiments, these scaffolds were capable of maintaining cell numbers and synthesizing glycosaminoglycans, indicating their potential application for in vitro regeneration of articular cartilage tissue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Lei Zhan, Lingtian Wang, Jixia Deng, Yi Zheng, Qinfei Ke, Xinrui Yang, Xing Zhang, Weitao Jia, Chen Huang
Summary: Nanogrooved microfiber scaffold shows improved cell adhesion and infiltration, making it important for tissue engineering and wound healing.
Review
Engineering, Biomedical
Drake D. Pedersen, Seungil Kim, William R. Wagner
Summary: Early explorations of tissue engineering and regenerative medicine commonly used simple polyesters as scaffolds. However, with the advancement of technology, a greater variety of synthetic biomaterials, such as biodegradable polyurethanes, have been employed. These polyurethanes can be customized based on their composition and processability, and can incorporate bioactive agents to achieve desired biological responses. While there is much enthusiasm and growth in the literature on biodegradable PU scaffolds, their regulatory approval and routine clinical use are still limited. Nonetheless, there is a growing body of research on the development and pre-clinical applications of these scaffolds.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Polymer Science
Lei Li, Pengfei Lu, Yuting Liu, Junhe Yang, Shengjuan Li
Summary: In this study, 3D bioactive glass/lignocellulose composite scaffolds were successfully fabricated by 3D-printing with NMMO as the ink solvent. The scaffolds showed uniform macropores and rough surfaces, with excellent mechanical properties and hydroxyapatite-forming capability. They also exhibited low cytotoxicity to human bone marrow mesenchymal stem cells. These 3D-printed BG/cellulose scaffolds have potential applications in bone tissue engineering.
Article
Materials Science, Biomaterials
Khalil Dayekh, Kibret Mequanint
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2020)
Article
Chemistry, Physical
Kalin Penev, Teshome Bedada, Ajay K. Ray, Kibret Mequanint
Article
Engineering, Biomedical
Khalil Dayekh, Kibret Mequanint
ACTA BIOMATERIALIA
(2020)
Article
Engineering, Chemical
Rebeca Arambula-Maldonado, Armin Geraili, Malcolm Xing, Kibret Mequanint
Summary: Tissue engineering and regenerative medicine have advanced significantly over the past 30 years, with key modules including cells, biodegradable scaffolds, bioreactors, and biomolecules. These fields involve various disciplines such as biological sciences, chemical engineering, materials chemistry, and polymer processing, requiring a deep understanding of viscous fluid flow, mass transfer, and reaction engineering.
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Multidisciplinary Sciences
Yicheng Guo, Ying Wang, Xiaohong Zhao, Xue Li, Quan Wang, Wen Zhong, Kibret Mequanint, Rixing Zhan, Malcolm Xing, Gaoxing Luo
Summary: The study introduces a novel visible light-induced blood-resistant hemostatic adhesive (HAD) that efficiently achieves hemostasis by activating and aggregating platelets. HAD demonstrates excellent hemostatic performance on liver incision and severed rat tail, significantly reducing blood loss.
Article
Cell Biology
Kathleen Zohorsky, Shigang Lin, Kibret Mequanint
Summary: The study demonstrated that utilizing bead-bound Jagged1 was effective in activating Notch3 and promoting SMC differentiation/maturation, while magnetic pulling forces did not activate Notch3 but instead provided necessary clustering or traction forces for Notch activation. The findings suggest that manipulation of Jagged1 presentation strategy can improve biomaterial-driven control of SMC behavior.
Article
Engineering, Biomedical
Leyu Wang, Yuqing Liu, Genlan Ye, Yutong He, Bingyun Li, Yezhi Guan, Baoyong Gong, Kibret Mequanint, Malcolm M. Q. Xing, Xiaozhong Qiu
Summary: Injectable and electrically conductive scaffolds with shape-memory behavior and hierarchical porous structure show promising results in functional repair of infarcted heart muscle in rats and minipigs, by increasing fractional shortening, ejection fraction, and reducing the infarcted area.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Engineering, Biomedical
Neda Aslankoohi, Shigang Lin, Kibret Mequanint
Summary: This study introduced dexamethasone-loaded organic-inorganic hybrid microparticles as a standalone osteogenic differentiation inducer. The microparticles supported cell adhesion and proliferation in culture, and induced osteogenic differentiation of stem cells without the need for any additional differentiation media supplements. Therefore, the fluorescent PEA-BG hybrid microparticles show promise as a standalone biomaterial for bone regeneration.
MATERIALS TODAY BIO
(2022)
Article
Polymer Science
Meng Wang, Shigang Lin, Kibret Mequanint
Summary: This study evaluated the potential of using synthesized polymers and electrospun scaffolds to enhance differentiation of iPSC-derived mesenchymal stem cells into smooth muscle cells, providing a feasible strategy for engineering functional vascular tissues.
Article
Multidisciplinary Sciences
Yuqing Liu, Ge Guan, Yinghao Li, Ju Tan, Panke Cheng, Mingcan Yang, Bingyun Li, Quan Wang, Wen Zhong, Kibret Mequanint, Chuhong Zhu, Malcolm Xing
Summary: This study proposes a hydrophobic adhesive UIHA that can perform in the presence of body fluids. The gelation of the hydrophobic fluid enables strong bonding to tissues and exceptional water resistance.
Article
Environmental Sciences
Fitfety M. Teshager, Nigus G. Habtu, Kibret Mequanint
Summary: This study demonstrates the efficient removal of cellulose-reactive anionic dyes using water hyacinth root powder (WHRP) bioadsorbent. By adjusting adsorption conditions such as pH, adsorbent dose, dye concentration, particle size, mixing speed, and temperature, the adsorption performance can be effectively enhanced. The adsorption process exhibits spontaneity and exothermicity in terms of Gibbs free energy and enthalpy, and is controlled by multiple mechanisms.
Article
Polymer Science
Kalin I. I. Penev, Matt Mulligan, Kibret Mequanint
Summary: This study aimed to minimize the dose rate effect of a tetrazolium salt dispersed within a gellan gum matrix by optimizing the concentrations of the salt and gum and adding various agents. The results showed successful minimization of the dose rate effect without sacrificing dosimeter integrity or sensitivity.
Review
Materials Science, Multidisciplinary
Rebeca Arambula-Maldonado, Kibret Mequanint
Summary: Electrically conductive polymers and carbon-based materials have great potential as biomaterials in bone tissue engineering. This review discusses the in vitro and in vivo performances of carbon-based conductive materials and their applications as scaffolds for bone tissue engineering, as well as summarizes the cellular processing mechanisms of these materials.
MATERIALS ADVANCES
(2022)
Review
Nanoscience & Nanotechnology
Armin Geraili, Malcolm Xing, Kibret Mequanint
Summary: Advanced polymeric controlled delivery systems are designed to treat chronic diseases by adjusting drug release profiles, but conventional carriers cannot meet the needs of diseases requiring variable drug release patterns. The importance of individualized therapy and the tunable properties of biodegradable polymers are driving the design and manufacturing of controlled drug delivery systems with adjustable release profiles.
Article
Nanoscience & Nanotechnology
Neda Aslankoohi, Kibret Mequanint
ACS APPLIED BIO MATERIALS
(2020)