Article
Biophysics
Md Danish Eqbal, Farha Naaz, Kajal Sharma, Venkat Gundabala
Summary: The development of a 3D glass-PDMS hybrid device allows for complete on-chip generation of cell-laden alginate beads in the presence of electric fields, with active control of droplet size. This approach successfully encapsulated E. coli cells into alginate microbeads and demonstrated promising technology for cell encapsulation with applications in various fields.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Applied
Joachim S. Kjesbu, Daria Zaytseva-Zotova, Sanna Samfors, Paul Gatenholm, Christofer Troedsson, Eric M. Thompson, Berit Lokensgard Strand
Summary: Alginate and cellulose nanofibrils (CNF) microbeads show promising applications in cell encapsulation, thanks to their high stability and biocompatibility. Crosslinking CNF with alginate improves the performance and cell viability of microbeads.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Multidisciplinary
Ik Sung Cho, Prerak Gupta, Nima Mostafazadeh, Sing Wan Wong, Saiumamaheswari Saichellappa, Stephen Lenzini, Zhangli Peng, Jae-Won Shin
Summary: The researchers developed a microfluidic approach to create single cells in compartmentalized 3D hydrogel matrices with tunable ligand presentation. They found that cells elongate with asymmetric presentation of integrin adhesion ligands, while they expand isotropically with symmetric presentation. Furthermore, they discovered that asymmetric ligand presentation influences the lineage commitment of stem cells. This study demonstrates the potential of precisely controlling 3D ligand presentation to direct cell polarity in regenerative engineering and medicine.
Article
Engineering, Multidisciplinary
Mehmet Ali Karaca, Derya Dilek Kancagi, Ugur Ozbek, Ercument Ovali, Ozgul Gok
Summary: Cell transplants in therapeutic studies cannot maintain long-term function inside the donor body. In this study, fibroblasts and MSCs were encapsulated inside alginate microbeads and showed improved viability and functionality during an incubation period of up to 12 days. The encapsulation strategy has the potential to be a promising cell delivery platform for cell therapy-based tissue engineering.
Article
Chemistry, Physical
Xinye Chen, Shuhuan Zhang, Yu Gan, Rui Liu, Ruo-Qian Wang, Ke Du
Summary: A microbead-packed deformable microfluidic system has been developed to efficiently separate plasma and blood cells, with a highly accurate model constructed to support experimental validation. This new device is expected to offer a new solution for centrifuge-free diagnosis and treatment of bloodborne diseases.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Ji Sun, Jingbo Li, Zhikun Huan, Stephen J. Pandol, Dechen Liu, Luoran Shang, Ling Li
Summary: Type 1 diabetes mellitus is characterized by autoimmune damage of pancreatic beta cells. This study develops mesenchymal stem cell (MSC)-laden composite beta cell porous microgels (MGs) to address sustainability for the treatment of diabetes. The results demonstrate that the MGs have great potential for the treatment of diabetes providing a promising platform for clinical beta cell transplantation.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Medicine, Research & Experimental
Adele Soltani, Masoud Soleimani, Mohammad Adel Ghiass, Seyed Ehsan Enderami, Shahram Rabbani, Arefeh Jafarian, Abdolamir Allameh
Summary: The study successfully differentiated insulin-producing cells from adipose-derived stem cells, improved cell survival using a microfluidic system, and effectively treated diabetes in experimental animals.
Article
Engineering, Biomedical
Onur Hasturk, Jordan A. Smiley, Miles Arnett, Jugal Kishore Sahoo, Cristian Staii, David L. Kaplan
Summary: The susceptibility of mammalian cells to harsh processing conditions limits their use in cell transplantation and tissue engineering. In this study, a method for fabricating Alg templated silk and silk/gelatin composite hydrogel microspheres using centrifugation-based droplet processing is demonstrated. The composite microspheres show improved structural stability and mechanical properties compared to ionically crosslinked Alg microspheres. Human mesenchymal stem and neural progenitor cells can be successfully encapsulated in these composite microspheres and protected from environmental factors.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Immunology
Hao Sun, Zhan Yang, Zhihai Teng, Yanping Zhang, Zhenwei Han, Chao Xu, Zhu Wang, Hu Wang, Hongzhuang Wen, Xiaodong Chen, Changbao Qu, Yaxuan Wang
Summary: Sertoli cell -only syndrome (SCOS) is a type of testicular pathological failure that causes male infertility. The molecular mechanism underlying its development remains unknown. DDX58 has been identified as a key gene in SCOS, and its upregulation in SCOS testicular Sertoli cells is positively correlated with the expression of inflammatory factors. Mechanistically, DDX58 acts as an RNA-binding protein that enhances p65 expression by promoting mRNA stability. The DDX58/p65 regulatory axis might be a therapeutic target for SCOS.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Peng Xiao, Jianpan Huang, Xiongqi Han, Jacinth W. S. Cheu, Yang Liu, Lok Hin Law, Joseph H. C. Lai, Jiyu Li, Se Weon Park, Carmen C. L. Wong, Raymond H. W. Lam, Kannie W. Y. Chan
Summary: The study reports a robust approach to image pH changes in the tumor microenvironment using pH-sensing microbeads. The method provides a sensitive and noninvasive way to monitor the pH changes during treatment.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Food Science & Technology
Yuanhang Yao, Jiaxing Jansen Lin, Xin Yi Jolene Chee, Mei Hui Liu, Saif A. Khan, Jung Eun Kim
Summary: The study demonstrates that microfluidic technology can effectively encapsulate lutein in food products, and both the type of oil and device do not affect the bioaccessibility of lutein.
Article
Nanoscience & Nanotechnology
Jin-Ok Chu, Yoon Choi, Do-Wan Kim, Hye-Seon Jeong, Jong Pil Park, David A. Weitz, Sei-Jung Lee, Hyomin Lee, Chang-Hyung Choi
Summary: This study reports the development of cell-inspired hydrogel microcapsules that effectively encapsulate and retain antioxidants. The microcapsules have an interstitial oil layer and an ionic gelation hydrogel shell, providing enhanced retention and protection of the antioxidants. Addition of a lipophilic reducing agent in the oil layer enhances the antioxidant activity, and lowering the storage temperature further improves the antioxidant activity.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Mohammad Suman Chowdhury, Xingcai Zhang, Leila Amini, Pradip Dey, Abhishek Kumar Singh, Abbas Faghani, Michael Schmueck-Henneresse, Rainer Haag
Summary: This study introduces an oxidation-responsive precursor for creating multifunctional surfactants, which can stabilize microfluidics-generated aqueous droplets, encapsulate different components, enable extraction of biomolecules, fabrication of microcapsules, and monitoring of single-cell gene expression. The development of multifunctional surfactants presents opportunities for a wide range of applications in adsorption, bioanalytics, catalysis, formulations, coatings, and programmable emulsions.
NANO-MICRO LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Jong Hyun Kim, Jong Bin Kim, Ye Hun Choi, Sanghyuk Park, Shin-Hyun Kim
Summary: Elastic photonic microbeads with enhanced color saturation are designed through facile photocuring of oil-in-oil emulsion droplets, offering potential as pragmatic structural colorants for various applications such as printings, paintings, and cosmetics. The high crystallinity of colloidal arrays increases reflectivity at stopband, while index matching reduces incoherent scattering, enhancing color saturation and fluidity.
Article
Chemistry, Analytical
Xiao Zhou, Yuanhang Mao, Miao Gu, Zhen Cheng
Summary: Researchers developed a microfluidic system embedded with a weakly supervised cell counting network (WSCNet) to generate microfluidic droplets, evaluate their quality, and accurately recognize the locations of encapsulated cells.
Article
Pharmacology & Pharmacy
Elisabetta Esposito, Maddalena Sguizzato, Markus Drechsler, Paolo Mariani, Federica Carducci, Claudio Nastruzzi, Rita Cortesi
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2017)
Article
Cell Biology
Francesca Mancuso, Mario Calvitti, Domenico Milardi, Giuseppe Grande, Giulia Falabella, Iva Arato, Stefano Giovagnoli, Federica Vincenzoni, Francesca Mancini, Claudio Nastruzzi, Maria Bodo, Tiziano Baroni, Massimo Castagnola, Riccardo Marana, Alfredo Pontecorvi, Riccardo Calafiore, Giovanni Luca
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2018)
Article
Toxicology
Francesca Mancuso, Iva Arato, Cinzia Lilli, Catia Bellucci, Maria Bodo, Mario Calvitti, Maria Chiara Aglietti, Marco dell'Omo, Claudio Nastruzzi, Riccardo Calafiore, Giovanni Luca, Lorella Marinucci
TOXICOLOGY IN VITRO
(2018)
Article
Polymer Science
Elisabetta Esposito, Maddalena Sguizzato, Christian Bories, Claudio Nastruzzi, Rita Cortesi
Article
Pharmacology & Pharmacy
Elisabetta Esposito, Alessandra Pecorelli, Maddalena Sguizzato, Markus Drechsler, Paolo Mariani, Federica Carducci, Franco Cervellati, Claudio Nastruzzi, Rita Cortesi, Giuseppe Valacchi
CURRENT DRUG DELIVERY
(2018)
Article
Materials Science, Multidisciplinary
Gabriele Pitingolo, Antoine Riaud, Claudio Nastruzzi, Valerie Taly
ADVANCED ENGINEERING MATERIALS
(2019)
Article
Biochemical Research Methods
Gabriele Pitingolo, Valerie Taly, Claudio Nastruzzi
Article
Chemistry, Analytical
Gabriele Pitingolo, Antoine Riaud, Claudio Nastruzzi, Valerie Taly
Review
Pharmacology & Pharmacy
Elisabetta Esposito, Claudio Nastruzzi, Maddalena Sguizzato, Rita Cortesi
CURRENT PHARMACEUTICAL DESIGN
(2019)
Article
Nanoscience & Nanotechnology
Elisabetta Esposito, Maddalena Sguizzato, Markus Drechsler, Paolo Mariani, Federica Carducci, Claudio Nastruzzi, Giuseppe Valacchi, Rita Cortesi
BEILSTEIN JOURNAL OF NANOTECHNOLOGY
(2019)
Article
Nanoscience & Nanotechnology
Silvia Mannucci, Federico Boschi, Barbara Cisterna, Elisabetta Esposito, Rita Cortesi, Claudio Nastruzzi, Enrica Cappellozza, Paolo Bernardi, Andrea Sbarbati, Manuela Malatesta, Laura Calderan
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2020)
Article
Pharmacology & Pharmacy
Fatih Yanar, Ali Mosayyebi, Claudio Nastruzzi, Dario Carugo, Xunli Zhang
Article
Chemistry, Analytical
Anna Nastruzzi, Gabriele Pitingolo, Giovanni Luca, Claudio Nastruzzi
Article
Medicine, Research & Experimental
Letizia Penolazzi, Elisabetta Lambertini, Stefania D'Agostino, Michela Pozzobon, Maria Pina Notarangelo, Pantaleo Greco, Pasquale De Bonis, Claudio Nastruzzi, Roberta Piva
Summary: The ability of degenerate intervertebral disc (IVD) cells to respond to physiological conditions in their niche, such as hypoxic environment and trophic factors, was investigated. Results showed that degenerate IVD cells were able to re-acquire discogenic phenotype and adapt to hypoxia after exposure to appropriate stimuli. This study opens new possibilities for engineering IVD by manipulating degenerated IVD cells to promote cell activity and repair.
Article
Materials Science, Multidisciplinary
Anna Nastruzzi, Franco Cicerchia, Annalisa Fortini, Claudio Nastruzzi
Summary: Infections acquired in public spaces pose a burden on health systems, and antimicrobial material surfaces play a role in infection prevention.
EMERGENT MATERIALS
(2021)