☆ 4.5 Article

14-3-3 Binding to Cyclin Y contributes to cyclin Y/CDK14 association

ACTA BIOCHIMICA ET BIOPHYSICA SINICA (2014)

期刊

ACTA BIOCHIMICA ET BIOPHYSICA SINICA

卷 46, 期 4, 页码 299-304

出版社

OXFORD UNIV PRESS

DOI: 10.1093/abbs/gmu005

关键词

cyclin Y; 14-3-3 protein; 14-3-3 binding motif

类别

Biochemistry & Molecular Biology Biophysics

资金

Ministry of Science and Technology of China [2010CB912103]
Science and Technology Commission of Shanghai Municipality [2JC1409300]

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Reagent

摘要

Cyclin Y is a highly conserved cyclin among eumetazoans, yet its function and regulation are poorly understood. To search for Cyclin Y-interacting proteins, we screened a yeast two-hybrid library using human Cyclin Y (CCNY) as a bait and identified the following interactors: CDK14 and four members of the 14-3-3 family (epsilon, beta, eta, tau). The interaction between CCNY and 14-3-3 proteins was confirmed both in vitro and in vivo. The results showed that Ser-100 and Ser-326 residues in CCNY were crucial for 14-3-3 binding. Interestingly, binding of CCNY to 14-3-3 significantly enhanced the association between CCNY and CDK14. Our findings may add a new layer of regulation of CCNY binding to its kinase partner.

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