Article
Cell Biology
Ling Li, Xiujuan Zhang, Yanni Lin, Xinxin Ren, Tian Xie, Jing Lin, Shumeng Wu, Qinong Ye
Summary: This study found that microRNA-let-7b-5p (let-7b-5p) represses the expression of Hexokinase 2 (HK2) by targeting its 3'-untranslated region. By suppressing HK2-mediated aerobic glycolysis, let-7b-5p restricts breast tumor growth and metastasis. In patients with breast cancer, let-7b-5p expression is significantly downregulated and is negatively correlated with HK2 expression. These findings suggest that the let-7b-5p/HK2 axis plays a key role in aerobic glycolysis as well as breast tumor proliferation and metastasis, and targeting this axis is a potential therapeutic strategy for breast cancer.
CELL DEATH DISCOVERY
(2023)
Article
Biology
Vennila Ponnusamy, Richard T. H. Ip, Moumin A. E. K. Mohamed, Paul Clarke, Eva Wozniak, Charles Mein, Leslie Schwendimann, Akif Barlas, Philippa Chisholm, Ela Chakkarapani, Adina T. Michael-Titus, Pierre Gressens, Ping K. Yip, Divyen K. Shah
Summary: Ponnusamy et al identified let-7b-5p as an elevated microRNA in neonates with moderate to severe encephalopathy using next generation sequencing of dried blood spots. They demonstrated that let-7b-5p exerts its effects through the Hippo-YAP pathway in cell culture and murine models of perinatal brain injury, indicating the need for validation in larger neonate cohorts to confirm its role as a biomarker for neonatal encephalopathy.
COMMUNICATIONS BIOLOGY
(2021)
Article
Environmental Sciences
Li-hua Dong, Jing-jing Huang, Peng Zu, Jing Liu, Xue Gao, Jian-wei Du, Yu-fu Li
Summary: The study revealed that circKDM4C is downregulated in AML patients, and overexpression of circKDM4C in AML cell lines can inhibit proliferation, migration, invasion, and promote ferroptosis. CircKDM4C acts as a sponge of hsa-let-7b-5p to regulate P53 expression in AML. CircKDM4C is negatively correlated with hsa-let-7b-5p, but positively correlated with P53 in AML patients.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yin Zhuang, Sheng Song, Dan Xiao, Xueguang Liu, Xiaofei Han, Shihao Du, Yuan Li, Yanming He, Shujun Zhang
Summary: The pathogenesis of intervertebral disc degeneration is still unclear. In this study, it was found that exosomes secreted by degenerative intervertebral disc-derived nucleus pulposus stem cells could exacerbate annulus fibrosus cell degeneration. This effect was mediated by the let-7b-5p miRNA, which inhibited cell proliferation, migration, and matrix synthesis, and promoted apoptosis. The activation of the IGF1R expression and PI3K-Akt pathway were also inhibited by let-7b-5p.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Oncology
Yujing Huang, Ying Liu, Jing Huang, Lu Gao, Zhenggang Wu, Lu Wang, Lin Fan
Summary: This study discovered that let-7b-5p promotes cell apoptosis in PD by targeting HMGA2, providing a potential theoretical basis for effective therapy in PD.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Cell Biology
Georgia Mandolesi, Francesca Romana Rizzo, Sara Balletta, Mario Stampanoni Bassi, Luana Gilio, Livia Guadalupi, Monica Nencini, Alessandro Moscatelli, Colleen Patricia Ryan, Valerio Licursi, Ettore Dolcetti, Alessandra Musella, Antonietta Gentile, Diego Fresegna, Silvia Bullitta, Silvia Caioli, Valentina Vanni, Krizia Sanna, Antonio Bruno, Fabio Buttari, Chiara Castelli, Carlo Presutti, Francesca De Santa, Annamaria Finardi, Roberto Furlan, Diego Centonze, Francesca De Vito
Summary: The study found that levels of let-7b-5p in cerebrospinal fluid of MS patients are correlated with various microRNAs and inflammation-related protein factors, showing specific expression patterns. Additionally, reduced CSF let-7b-5p levels were associated with progressive MS and negatively correlated with hallmark processes of the disease phases, indicating potential use as a biomarker for MS course.
Article
Cell Biology
Federica Scaroni, Caterina Visconte, Maria Serpente, Maria Teresa Golia, Martina Gabrielli, Marijn Huiskamp, Hanneke E. Hulst, Tiziana Carandini, Milena De Riz, Anna Pietroboni, Emanuela Rotondo, Elio Scarpini, Daniela Galimberti, Charlotte E. Teunissen, Maureen van Dam, Brigit A. de Jong, Chiara Fenoglio, Claudia Verderio
Summary: This study identifies miR-150-5p and let-7b-5p packaged in blood myeloid EVs as possible biomarkers for cognitive deficits in MS.
Article
Medicine, General & Internal
Yadi Zhang, Sihui Tang, Wanchun Yang, Fangbing Du
Summary: let-7b-5p inhibits the proliferation and migration of PDGF-induced PASMCs by modulating IGF1.
Article
Biotechnology & Applied Microbiology
Sezin Aday, Inbal Hazan-Halevy, Aranzazu Chamorro-Jorganes, Maryam Anwar, Meir Goldsmith, Nicholas Beazley-Long, Susmita Sahoo, Navneet Dogra, Walid Sweaad, Francesco Catapano, Sho Ozaki-Tan, Gianni D. Angelini, Paolo Madeddu, Andrew V. Benest, Dan Peer, Costanza Emanueli
Summary: miRNAs regulate gene expression by post-transcriptional inhibition, sEVs with miRNAs promote angiogenesis, LNPs are used for RNA therapeutics delivery. This study identified and developed bioinspired artificial exosomes containing proangiogenic miRNA, showing potential in promoting angiogenesis.
Article
Genetics & Heredity
Wanjin Chen, Haibo Wang, Yuanlong Shen, Shouwen Wang, Deng Liu, Hongchuan Zhao, Guobin Wang, Fan Huang, Wei Wang, Ruolin Wu, Liujin Hou, Zhenghui Ye, Xinghua Zhang, Xiaoping Geng, Xiaojun Yu
Summary: The aim of this study was to investigate the effect of let-7c-5p on hepatocellular carcinoma (HCC) and its molecular pathway. The expression level of let-7c-5p was reduced in HCC and was associated with shorter survival time of HCC patients. Let-7c-5p inhibited HCC cell proliferation, invasion, and migration, and promoted apoptosis. CDCA8 was identified as a downstream mRNA of let-7c-5p that was negatively regulated by it. CDCA8 was found to be related to immune cells and immune pathways. Let-7c-5p suppresses HCC by down-regulating CDCA8, contributing to the understanding of HCC pathogenesis and drug development.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Immunology
Yao Wang, Xi Jia, Ling Zhou, Jie Yin, Yunxiao Zhao, Lan Dai, Bin Zuo, Yang He
Summary: The study found that let-7b-5p expression was elevated in B cells of ITP patients and B cell survival was enhanced. BAFF and B cell receptor stimulation can induce the expression of let-7b-5p in vitro. Overexpression of let-7b-5p in B cells enhances cell survival and promotes BAFF-R expression.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Psychiatry
Sanne Roumans, Kristina Sundquist, Ashfaque A. Memon, Anna Hedelius, Jan Sundquist, Xiao Wang
Summary: The study found that lower plasma levels of let-7b-5p are associated with a higher future risk of MDD. These results need validation in a large cohort to explore its potential as a peripheral biomarker for MDD.
Article
Multidisciplinary Sciences
Katja Koeppen, Amanda Nymon, Roxanna Barnaby, Laura Bashor, Zhongyou Li, Thomas H. Hampton, Amanda E. Liefeld, Fred W. Kolling, Ian S. LaCroix, Scott A. Gerber, Deborah A. Hogan, Swetha Kasetty, Carey D. Nadell, Bruce A. Stanton
Summary: The study demonstrates that microRNAs in extracellular vesicles secreted by human airway epithelial cells can regulate protein expression, antibiotic sensitivity, and biofilm formation by Pseudomonas aeruginosa. This bidirectional interdomain communication provides direct evidence for transfer of miRNAs in EVs from eukaryotic cells to prokaryotes, resulting in phenotypic alterations in the prokaryote. The findings suggest potential clinical applications for using let-7b-5p and a beta-lactam antibiotic in nanoparticles or EVs to benefit patients with antibiotic-resistant P. aeruginosa infections.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Si-Yang Wang, Yue Xu, Quan Hong, Xiang-Mei Chen, Guang-Yan Cai
Summary: This study demonstrated that aged mice are more susceptible to AKI injury and identified an important role for the upregulation of the senescence-associated DNA damage/p53 pathway in this susceptibility. MSC treatment can reduce the degree of DNA damage and apoptosis. Furthermore, downregulation of let-7b-5p may lead to increased renal AKI susceptibility, and the presence of let-7b-5p in MSC-derived Exos can alleviate tubular epithelial cell apoptosis by inhibiting p53.
CELL AND TISSUE RESEARCH
(2022)
Article
Oncology
Teeranut Asavasupreechar, Ryoko Saito-Koyama, Yasuhiro Miki, Keiichi Tamai, Jiro Abe, Chihiro Inoue, Ikuro Sato, Viroj Boonyaratanakornkit, Hironobu Sasano
Summary: This study revealed that the presence of progesterone receptor (PR) and PR isoform B (PRB) is correlated with better clinical outcomes in breast cancer and non-small cell lung cancer (NSCLC). The microRNA let-7b can inhibit cell proliferation by inducing PR and PRB expression in breast cancer cell lines. The let-7b-E2F1 interaction was found to be responsible for the inhibition of PRB in breast cancer cells.
ENDOCRINE-RELATED CANCER
(2023)
Article
Cell Biology
Irene Soffritti, Maria D'Accolti, Gloria Ravegnini, Maria-Cristina Arcangeletti, Clara Maccari, Flora De Conto, Adriana Calderaro, Elisabetta Caselli
Summary: Viral infections, particularly by HCMV and HHV-6, may play a role in the onset and progression of systemic sclerosis by modulating miRNA expression and causing cell fibrosis, potentially serving as biomarkers for SSc diagnosis, assessment of disease progression, and antifibrotic therapies.
Review
Medicine, General & Internal
Antonio De Leo, Donatella Santini, Claudio Ceccarelli, Giacomo Santandrea, Andrea Palicelli, Giorgia Acquaviva, Federico Chiarucci, Francesca Rosini, Gloria Ravegnini, Annalisa Pession, Daniela Turchetti, Claudio Zamagni, Anna Myriam Perrone, Pierandrea De Iaco, Giovanni Tallini, Dario de Biase
Summary: Ovarian carcinomas are a heterogeneous group of neoplasms with different types and characteristics. The WHO classifies them into five distinct types, each with unique molecular features. Advances in molecular pathology have improved our understanding of ovarian carcinomas and can guide personalized clinical decisions.
Review
Endocrinology & Metabolism
Cecilia Catellani, Gloria Ravegnini, Chiara Sartori, Sabrina Angelini, Maria E. Street
Summary: Growth hormone and the insulin-like growth factor system play crucial roles in cell proliferation, differentiation, apoptosis, and angiogenesis, while miRNAs and lncRNAs regulate gene expression and have been linked to diseases such as cancer.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Oncology
Federica D'Amico, Anna Myriam Perrone, Simone Rampelli, Sara Coluccelli, Monica Barone, Gloria Ravegnini, Marco Fabbrini, Patrizia Brigidi, Pierandrea De Iaco, Silvia Turroni
Summary: This pilot study on the trajectory of gut microbiota in patients with epithelial ovarian cancer undergoing chemotherapy found distinct changes in the microbiota of platinum-resistant patients, potentially linked to therapeutic failure. In contrast, platinum-sensitive patients showed more diverse and stable microbiota, which may be beneficial for treatment.
Review
Oncology
Gloria Ravegnini, Francesca Gorini, Eugenia De Crescenzo, Antonio De Leo, Dario De Biase, Marco Di Stanislao, Patrizia Hrelia, Sabrina Angelini, Pierandrea De Iaco, Anna Myriam Perrone
Summary: Endometrial cancer is the most common gynecological cancer with challenging diagnostic and prognostic features. miRNAs play an important role in EC but there is currently lack of consensus in studies. Future research needs to explore more diagnostic and prognostic biomarkers and address the challenges in the current classification schemes.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Review
Oncology
Giulia Dondi, Eleonora Porcu, Alessandra De Palma, Giuseppe Damiano, Eugenia De Crescenzo, Linda Cipriani, Maria Dirodi, Gloria Ravegnini, Antonio De Leo, Margherita Nannini, Martina Ferioli, Alessio G. Morganti, Maria Abbondanza Pantaleo, Pierandrea De Iaco, Anna Myriam Perrone
Summary: Uterine sarcomas, rare cancers sometimes diagnosed in women of childbearing age, may be treated using fertility-sparing treatments in selected early stage cases. The outcomes of such treatments are influenced by the histology of the tumor, with leiomyosarcoma showing the worst prognosis and highest death rate among the subtypes mentioned in the systematic review.
Article
Oncology
Gloria Ravegnini, Antonio De Leo, Camelia Coada, Francesca Gorini, Dario de Biase, Claudio Ceccarelli, Giulia Dondi, Marco Tesei, Eugenia De Crescenzo, Donatella Santini, Angelo Gianluca Corradini, Giovanni Tallini, Patrizia Hrelia, Pierandrea De Iaco, Sabrina Angelini, Anna Myriam Perrone
Summary: The study found that miR-499a-3p and miR-499a-5p were overexpressed in CTNNB1 mutant EC patients, while NSMP patients with low miR-499a-5p expression had longer overall survival. Combining CTNNB1 status and miR-499a-5p levels allows better stratification of NSMP patients and may promote personalized treatment in intermediate-risk patients.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Maria A. Pantaleo, Milena Urbini, Angela Schipani, Margherita Nannini, Valentina Indio, Antonio De Leo, Bruno Vincenzi, Antonella Brunello, Giovanni Grignani, Mariaelena Casagrande, Elena Fumagalli, Elena Conca, Maristella Saponara, Elisa Gruppioni, Annalisa Altimari, Dario De Biase, Giovanni Tallini, Gloria Ravegnini, Daniela Turchetti, Marco Seri, Andrea Ardizzoni, Paola Secchiero, Annalisa Astolfi
Summary: Germline SDHA variants are highly prevalent in SDHA-deficient GIST, and the disease may occur in older adults as well. Genetic testing and surveillance should be conducted for SDHA mutation carriers and their relatives.
FRONTIERS IN ONCOLOGY
(2022)
Article
Medicine, General & Internal
Camelia Alexandra Coada, Giulia Dondi, Gloria Ravegnini, Antonio De Leo, Donatella Santini, Eugenia De Crescenzo, Marco Tesei, Alessandro Bovicelli, Susanna Giunchi, Ada Dormi, Marco Di Stanislao, Alessio G. Morganti, Dario De Biase, Pierandrea De Iaco, Anna Myriam Perrone
Summary: Endometrial cancer is the most common gynecological malignancy. Risk stratification should be based on the integration of histologic, clinical, and molecular parameters.
Review
Biochemistry & Molecular Biology
Gloria Ravegnini, Pierandrea De Iaco, Francesca Gorini, Giulia Dondi, Isabella Klooster, Eugenia De Crescenzo, Alessandro Bovicelli, Patrizia Hrelia, Anna Myriam Perrone, Sabrina Angelini
Summary: Epithelial ovarian cancer is highly lethal due to nonspecific symptoms and lack of screening tests, leading to delayed diagnosis and treatment. The current clinical biomarker CA-125 is not sensitive or specific enough to monitor chemotherapy response or predict relapse risk. Thus, identifying circulating miRNAs associated with therapeutic treatments is crucial for personalized patient management.
Review
Health Care Sciences & Services
Gloria Ravegnini, Martina Ferioli, Alessio Giuseppe Morganti, Lidia Strigari, Maria Abbondanza Pantaleo, Margherita Nannini, Antonio De Leo, Eugenia De Crescenzo, Manuela Coe, Alessandra De Palma, Pierandrea De Iaco, Stefania Rizzo, Anna Myriam Perrone
Summary: The contribution of radiomics and AI in the radiological preoperative assessment of patients with uterine sarcomas (USs) is still insufficiently supported by evidence. While promising, the quality of studies should be a top priority in the utilization of these new technologies.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Gloria Ravegnini, Margherita Nannini, Valentina Indio, Cesar Serrano, Francesca Gorini, Annalisa Astolfi, Aldo Di Vito, Fabiana Morroni, Maria Abbondanza Pantaleo, Patrizia Hrelia, Sabrina Angelini
Summary: GISTs with PDGFRA D842V mutation show differential miRNA expression compared to non-D842V mutated GISTs. Differentially expressed miRNAs mainly target genes involved in the immune response pathways. Additionally, the miRNA-mRNA network reveals the indolent behavior of D842V mutated GISTs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Health Care Sciences & Services
Martina Ruscelli, Thais Maloberti, Angelo Gianluca Corradini, Francesca Rosini, Giulia Querzoli, Marco Grillini, Annalisa Altimari, Elisa Gruppioni, Viviana Sanza, Alessia Costantino, Riccardo Ciudino, Matteo Errani, Alessia Papapietro, Sara Coluccelli, Daniela Turchetti, Martina Ferioli, Susanna Giunchi, Giulia Dondi, Marco Tesei, Gloria Ravegnini, Francesca Abbati, Daniela Rubino, Claudio Zamagni, Emanuela D'Angelo, Pierandrea De Iaco, Donatella Santini, Claudio Ceccarelli, Anna Myriam Perrone, Giovanni Tallini, Dario de Biase, Antonio De Leo
Summary: This study aims to evaluate the impact of integrated molecular and pathologic risk stratification in the clinical practice of endometrial carcinoma (EC). It found that molecular classes and risk groups were correlated with disease-free survival. The study supports the prognostic importance of EC molecular classification and the essential role of histopathologic assessment in patients' management.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Oncology
Camelia Alexandra Coada, Miriam Santoro, Vladislav Zybin, Marco Di Stanislao, Giulia Paolani, Cecilia Modolon, Stella Di Costanzo, Lucia Genovesi, Marco Tesei, Antonio De Leo, Gloria Ravegnini, Dario De Biase, Alessio Giuseppe Morganti, Luigi Lovato, Pierandrea De Iaco, Lidia Strigari, Anna Myriam Perrone
Summary: This study investigated the potential of radiomic features extracted from pre-surgical CT scans to predict disease-free survival (DFS) in endometrial cancer (EC) patients. Machine learning models achieved high sensitivities and specificities in both training and test sets, showing promise for predicting EC recurrence.
Review
Oncology
Federica Medici, Stefania Rizzo, Milly Buwenge, Alessandra Arcelli, Martina Ferioli, Gabriella Macchia, Francesco Deodato, Savino Cilla, Pierandrea De Iaco, Anna Myriam Perrone, Silvia Strolin, Lidia Strigari, Gloria Ravegnini, Alberto Bazzocchi, Alessio G. Morganti
Summary: Sarcopenia is a syndrome characterized by age-related loss of skeletal muscle mass and function, worsens radiation-induced toxicity and prognosis, and has a negative impact on the quality of life. Myosteatosis, a pathological infiltration of muscle tissue by adipose tissue, is correlated with prognosis in cancer patients. Sarcopenic obesity, characterized by large fat mass, is independently associated with higher mortality and worse complications compared to sarcopenia alone. Assessment of sarcopenia is commonly done using CT images. Several studies have shown the negative impact of sarcopenia on overall survival in patients undergoing radiation therapy for various tumors. Further research is needed to find ways to prevent and manage sarcopenia during and after radiation therapy in both adult and pediatric patients.