Article
Chemistry, Multidisciplinary
Victoria E. Paluzzi, Cuizheng Zhang, Chengde Mao
Summary: Small ssDNA circles are difficult to prepare, as they often form concatemers instead of monomeric circles. This study presents a simple method to overcome this problem by designing complementary linker molecules. The method allows for the enzymatic ligation of ssDNA as short as 16 nts into monomeric circles at high concentration and high yield.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Qun Ma, Boqiang He, Guojin Tang, Ran Xie, Peng Zheng
Summary: The immobilization of proteins on nanoparticles has gained attention in recent years. Enzymatic protein immobilization, particularly using the peptide ligase OaAEP1, shows advantages due to site-specific connection. Our study demonstrates the successful C-terminal site-specific protein immobilization on amino-functionalized Fe3O4 nanoparticles mediated by OaAEP1. This provides a new method for site-specific protein immobilization on nanoparticles.
Article
Chemistry, Analytical
Liang-Yong Yang, Xiong-Wei Xu, Yan Lin, Chen-Liu Ye, Wei-Qiang Liu, Zhou-Jie Liu, Guang-Xian Zhong, Yan-Fang Xu, Xin-Hua Lin, Jin-Yuan Chen
Summary: This study developed a nucleic acid template-dominated click chemistry-mediated ligation chain reaction (cc-eLCR) for efficient and specific nucleic acid amplification. The cc-eLCR method, which utilized nM-level DNA probes and an electrochemical platform, successfully detected low-level nucleic acid targets with single-base resolution. Moreover, the cc-eLCR assay was also applied to detect target molecules in cell extracts, showing its potential in medical diagnosis and biomedical research.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Huan Zhang, Yuhong Cao, Dawei Xu, Natalie S. Goh, Gozde S. Demirer, Stefano Cestellos-Blanco, Yuan Chen, Markita P. Landry, Peidong Yang
Summary: The study synthesizes PEI-AuNCs for delivering siRNA into intact plants, achieving efficient gene knockdown without being hindered by the plant cell wall, providing a new approach for broad applications in plant biotechnology.
Article
Chemistry, Multidisciplinary
Alejandro J. Da Silva Sanchez, Curtis Dobrowolski, Ana Cristian, Elisa Schrader Echeverri, Kun Zhao, Marine Z. C. Hatit, David Loughrey, Kalina Paunovska, James E. Dahlman
Summary: In order to predict the effectiveness of lipid nanoparticle (LNP) delivery systems in humans, researchers have investigated whether the mechanism of LNP uptake into cells is consistent across different species. Studies have shown that besides the interaction between apolipoprotein E (ApoE) and low-density lipoprotein receptor (LDLR), other interactions may also affect the targeting ability of LNPs.
Article
Engineering, Environmental
Xiu Han, Yan Lu, Haiping Wu, Xiaoxiang Guan, Xueping Ma, Yanan Chu, Bingjie Zou, Guohua Zhou
Summary: In this study, a prism oligonucleotide nanoparticle (pONP)-based delivery system was designed for the specific release of siRNA into telomerase-positive cancer cells. Both in vitro and in vivo tests confirmed the high precision and effectiveness of this nanoparticle cage in delivering siRNA. This delivery method shows great promise for targeted siRNA release into cancer cells.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Michiko Kimoto, Si Hui Gabriella Soh, Hui Pen Tan, Itaru Okamoto, Ichiro Hirao
Summary: Our study demonstrates the cognate base pair selectivity of T4 DNA ligase using a hydrophobic unnatural base pair (Ds-Pa) for template-dependent ligation. The efficient recognition of Ds-Pa pairing and exclusion of noncognate pairings by Ds indicate higher selectivity compared to natural base pairings. This hydrophobic base pairing can be utilized in enzymatic ligation for genetic alphabet expansion, paving the way for the creation of semi-synthetic organisms containing unnatural base pairs.
Article
Chemistry, Analytical
Wanling Cui, Xiaoyang Fan, Congcong Shen, Qingshuai Chen, Xiumei Zhang, Enguang Lv, Chonghui Li, Qiang Li, Rui Wang, Shicai Xu, Jihua Wang
Summary: In this study, a multiple primers-mediated exponential rolling circle amplification strategy was proposed for label-free and highly sensitive fluorescent analysis of T4 PNK and T4 DNA ligase activity. The strategy showed extremely high detection sensitivity and exhibited excellent inhibitor evaluation effects. Moreover, it could detect T4 PNK in HeLa cell lysate, indicating its potential application in disease diagnosis and treatment evaluation.
MICROCHEMICAL JOURNAL
(2022)
Article
Engineering, Biomedical
Yu Xia, Guoyi Tang, Yi Chen, Changbing Wang, Min Guo, Tiantian Xu, Mingqi Zhao, Yongjian Zhou
Summary: RNA interference (RNAi) is a promising method for treating malignant tumors, and in this study, RGDfC-SeNPs were successfully synthesized as a tumor-targeted carrier for delivering siSox2 to HepG2 liver cancer cells. The results showed that RGDfC-Se@siSox2 effectively silenced Sox2, inhibited tumor cell proliferation, migration, and invasion, induced apoptosis through reactive oxygen species production and mitochondrial membrane potential disruption, and significantly inhibited tumor growth in mice bearing HepG2 tumors. These findings suggest that RGDfC-SeNPs could be a valuable gene carrier for specific gene-targeted therapy in HCC.
BIOACTIVE MATERIALS
(2021)
Article
Chemistry, Physical
Elzbieta Pedziwiatr-Werbicka, Michal Gorzkiewicz, Sylwia Michlewska, Maksim Ionov, Dzmitry Shcharbin, Barbara Klajnert-Maculewicz, Cornelia E. Pena-Gonzalez, Javier Sanchez-Nieves, Rafael Gomez, F. Javier de la Mata, Maria Bryszewska
Summary: Gene therapy is a promising approach for treating diseases, but efficient delivery systems for nucleic acids are necessary. This study evaluated the use of gold nanoparticles as siRNA delivery systems, showing that dendronization of AuNPs could be a promising way to develop carriers for anticancer gene therapy.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Analytical
Bingzhi Li, Bin Zhou, Yue Chen, Xing Zhang, He Huang
Summary: In this study, a new strategy was proposed to sensitively detect transcription factors using T4 DNA ligase protection assay and rolling circle amplification. By preventing the ligation reaction through the binding of T4 DNA ligase to the nick region of dsDNA, the undesired dissociation of TF-DNA complexes was effectively avoided, improving the accuracy of the assay. Through sequence engineering, multiple transcription factors can be detected at low picomolar levels.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Esther H. Roh, Thomas H. Epps, Millicent O. Sullivan
Summary: The article introduces a mathematical framework that can predict siRNA-mediated gene silencing with minimal experimental data and guide nanocarrier design. It reveals that siRNA dilution is a key determinant of gene silencing kinetics, and the experimental and predicted results align closely in vitro and in vivo settings.
Article
Genetics & Heredity
Fatma Uysal, Ozgur Cinar, Alp Can
Summary: DNA methylation, regulated by Dnmt1 and Dnmt3a enzymes, plays a critical role in preimplantation embryo development. Knockdown of Dnmt1 and Dnmt3a resulted in embryos arresting at early stages, with different effects on global DNA methylation levels. Additionally, the distinct subcellular localization of Dnmt1 and Dnmt3a may contribute to their specific functions during embryonic development.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Oncology
Mohammad Heiat, Ehsan Rezaei, Javad Gharechahi, Masoumeh Abbasi, Javad Behroozi, Mohammad Ali Abyazi, Behzad Baradaran
Summary: Silencing SIX4 inhibits the survival, migration, and invasion ability of pancreatic cancer cells, increases apoptosis and autophagy rates, and reduces mitochondrial membrane potential. Furthermore, knockdown of SIX4 affects the expression of genes related to apoptosis and metastasis. Overall, SIX4 plays a critical role in the development of pancreatic cancer.
Article
Polymer Science
Beste Cagdas Tunali, Omer Akturk, Durukan Sahingoz, Mustafa Turk, Ayten Celebi Keskin
Summary: In this study, polyethyleneimine-capped gold nanoparticles (PEI-AuNPs) were synthesized using the pulsed laser ablation in liquid (PLAL) technique, and then combined with Bombyx mori silk fibroin (SF) to form nanocomposites (PEI-SF-AuNPs). The biocompatibility of PEI-AuNPs and PEI-SF-AuNPs in breast cancer cells MCF-7 and MDA-MB231 were tested, and it was found that PEI-AuNP1 showed the least cytotoxicity. Therefore, PEI-AuNP1 nanocomposites can be suggested as siRNA carriers in gene-based breast cancer therapies.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Chemistry, Analytical
Isaac N. Larkin, Viswanadham Garimella, Gokay Yamankurt, Alexander W. Scott, Hang Xing, Chad A. Mirkin
ANALYTICAL CHEMISTRY
(2020)
Review
Chemistry, Multidisciplinary
Guoqiang Liu, Sarah Hurst Petrosko, Zijian Zheng, Chad A. Mirkin
Article
Chemistry, Physical
Seung-Hoon Lee, Taegon Oh, Jehyeok Ryu, Chad A. Mirkin, Jae-Won Jang
CHEMISTRY OF MATERIALS
(2020)
Article
Chemistry, Multidisciplinary
Peng-Cheng Chen, Yuan Liu, Jingshan S. Du, Brian Meckes, Vinayak P. Dravid, Chad A. Mirkin
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Immunology
Lei Qin, Shuya Wang, Donye Dominguez, Alan Long, Siqi Chen, Jie Fan, Jihae Ahn, Kacper Skakuj, Ziyin Huang, Andrew Lee, Chad Mirkin, Bin Zhang
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Lin Sun, Haixin Lin, Yuanwei Li, Wenjie Zhou, Jingshan S. Du, Chad A. Mirkin
ADVANCED MATERIALS
(2020)
Article
Chemistry, Multidisciplinary
Bo Shen, Liliang Huang, Jiahong Shen, Lingyuan Meng, Edward J. Kluender, Chris Wolverton, Bozhi Tian, Chad A. Mirkin
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Chemistry, Multidisciplinary
Seokhyoung Kim, Cindy Y. Zheng, George C. Schatz, Koray Aydin, Kyoung-Ho Kim, Chad A. Mirkin
Article
Multidisciplinary Sciences
Wenjie Zhou, Zizhuo Liu, Ziyin Huang, Haixin Lin, Devleena Samanta, Qing-Yuan Lin, Koray Aydin, Chad A. Mirkin
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Jingshan S. Du, Donghoon Shin, Teodor K. Stanev, Chiara Musumeci, Zhuang Xie, Ziyin Huang, Minliang Lai, Lin Sun, Wenjie Zhou, Nathaniel P. Stern, Vinayak P. Dravid, Chad A. Mirkin
Article
Chemistry, Multidisciplinary
Zachary J. Urbach, Sarah S. Park, Steven L. Weigand, James E. Rix, Byeongdu Lee, Chad A. Mirkin
Summary: The study demonstrates that in a magnetic field, cubic Fe3O4 nanoparticles functionalized with DNA can exhibit unexpected crystallographic alignment of the entire superlattice, expanding the potential of crystal engineering with DNA through physical and chemical control.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Ho Fung Cheng, Shunzhi Wang, Chad A. Mirkin
Summary: Recent studies have shown that PAEs can behave as electron equivalents at small size and low DNA grafting density, however, the polydispersity in the number of DNA strands on the surfaces of these NPs limits the structural uniformity and tailorability of NP-based EEs. By assembling molecularly precise EEs with complementary NP-based PAEs, three distinct metallic phases can be formed, demonstrating that molecular-based EEs can influence specific phase formation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Editorial Material
Chemistry, Multidisciplinary
Jingshan S. Du, Wenjie Zhou, Sara M. Rupich, Chad A. Mirkin
Summary: The structure of MTPs is determined by specific crystallographic features, and their formation pathways include growth from multiply twinned seeds or stepwise formation of new twin boundaries on single-crystalline seeds. Recent studies have utilized in situ transmission electron microscopy to provide real-time evidence for both pathways, which will aid in the design of robust MTP syntheses. Further research on the structural evolution and energetics of nanoparticles is needed for a complete understanding of MTP formation pathways.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Inorganic & Nuclear
Ho Fung Cheng, McKinley K. Paul, Andrea D'Aquino, Charlotte L. Stern, Chad A. Mirkin
Summary: The study reveals that in a multiphasic solvent environment, dynamic weak-link approach (WLA) complexes demonstrated the ability to shuttle between aqueous and organic phases and modulate their denticity as they partition into different phases. This highly reconfigurable property of WLA complexes can be utilized to design biphasic reaction networks or chemical separations driven by straightforward salt metathesis reactions.
INORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Sasha B. Ebrahimi, Devleena Samanta, Benjamin E. Partridge, Caroline D. Kusmierz, Ho Fung Cheng, Arabela A. Grigorescu, Jorge L. Chavez, Peter A. Mirau, Chad A. Mirkin
Summary: Researchers have developed a strategy based on forced intercalation (FIT) to transduce subtle changes in aptamer structures into fluorescent readouts, expanding the scope of aptamer recognition for detecting steroids. This technology offers advantages such as rapid readout, simple instrumentation, and low sample volumes for studying steroids in clinical samples.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
