期刊
ACS NANO
卷 6, 期 10, 页码 8665-8673出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn302202t
关键词
dielectrophoresis; force spectroscopy; intermolecular interactions; microfluidic device; quasi-equilibrium unbinding events; high-force pulling assay
类别
资金
- Korean Ministry of Education and Science [NRF-2010-0026223, NRF-2010-0027238, NRF-2010-0013619, NRF-2009-0068841]
- University of Illinois at Urbana-Champaign
The simultaneous investigation of a large number of events with different types of intermolecular interactions, from nonequilibrium high-force pulling assays to quasi-equilibrium unbinding events in the same environment, can be very important for fully understanding intermolecular bond-rupture mechanisms. Here, we describe a novel dielectrophoretic force spectroscopy technique that utilizes microsized beads as multifunctional probes for parallel measurement of intermolecular forces with an extremely wide range of force rate (10(-4) to 10(4) pN/s) inside a microfluidic device. In our experiments, various forces, which broadly form the basis of all molecular interactions, were measured across a range of force loading rates by multifunctional probes of various diameters with a throughput of over 600 events per mm(2), simultaneously and in the same environment. Furthermore, the individual bond-rupture forces, the parameters for the characterization of entire energy landscapes, and the effective stiffness of the force spectroscopy were determined on the basis of the measured result;. This method of determining intermolecular forces could be very useful for the precise and simultaneous examination of various molecular interactions, as it can be easily and cost-effectively implemented within a microfluidic device for a range of applications including immunoassays, molecular mechanics, chemical and biological screening, and mechanobiology.
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