Article
Optics
Ping Liu, Yuechao Jiao, Xuzhao Chai, Yanxia Ma, Sheng Liu, Xiangqian Fang, Fuling Fan, Li Xue, Jianxun Han, Qin Liu
Summary: This article comprehensively summarizes the latest advances in single-walled carbon nanotube (SWCNT)-based field effect transistor (FET) and optical biosensors. The basic structure and physical properties of SWCNTs are introduced, followed by a systematic summary of the recent developments in SWCNT-FET biosensors and optical biosensors. The current challenges in SWCNT synthesis and bio-sensing devices are discussed, and future perspectives are provided for this rapidly developing field.
JOURNAL OF LUMINESCENCE
(2022)
Article
Chemistry, Multidisciplinary
Naveed A. Bakh, Xun Gong, Michael A. Lee, Xiaojia Jin, Volodymyr B. Koman, Minkyung Park, Freddy T. Nguyen, Michael S. Strano
Summary: This study developed a nanosensor platform for selective molecular recognition of ascorbic acid and riboflavin in a murine model, demonstrating reproducible and stable results even after exposure to mouse serum for three days, and achieving an order of magnitude improvement in p-value during analyte sensing by using a geometrical encoding scheme to correct for optical and mechanical artifacts.
Article
Nanoscience & Nanotechnology
Matthew Card, Mitchell Gravely, S. Zahra M. Madani, Daniel Roxbury
Summary: Single-walled carbon nanotubes (SWCNTs) have great potential for single molecule detection, but conventional glass coverslips can adversely affect their fluorescence properties. Research shows that using a hydrogel platform can significantly reduce fluorescence deviation in SWCNTs and enable localized molecule detection.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Nanoscience & Nanotechnology
Mitchell Gravely, Aidan Kindopp, Lauren Hubert, Matthew Card, Aceer Nadeem, Christopher Miller, Daniel Roxbury
Summary: The non-covalent biomolecular functionalization of fluorescent single-walled carbon nanotubes (SWCNTs) has led to their extensive use in in vitro and in vivo sensing and imaging applications. However, it has been observed that these SWCNTs tend to aggregate in protein-containing environments before interacting with cells at the nanobiointerface. This study shows that initially aggregated SWCNTs do not further accumulate within subcellular locations and that SWCNTs with a low degree of aggregation can induce significant deleterious effects on cells.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Verena Wulf, Gili Bisker
Summary: The incorporation of SWCNTs as fluorescent probes enables real-time monitoring of the morphology and time-dependent structural changes of Fmoc-diphenylalanine hydrogels. Spectral modulations of SWCNT-fluorescence allow for the observation of structural changes induced by cross-linking ions and polymer additives in the hydrogels.
Article
Chemistry, Multidisciplinary
Yui Konno, Michio Yamada, Mitsuaki Suzuki, Yutaka Maeda
Summary: Functionalization of single-walled carbon nanotubes (SWCNTs) alters the near-infrared photoluminescence wavelength and emission efficiency. The binding configuration and degree of functionalization affect these modifications, and controlling the degree of functionalization is crucial. A stepwise approach combining SWCNTs functionalization and subsequent reactions can effectively control their photoluminescence properties. This study focused on the reductive alkylation of SWCNTs using bromoalkanes with Boc-protected amino groups and subsequent deprotection and amidation reactions to understand the effects of different reagents on SWCNTs' properties.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Physical
Yutao Zhang, Yahui Li, Haijian Wen, Leitao Cao, Wenxin Xiao, Jintao Zhao, Zeyuan Nan, Suxia Zhang, Song Qiu, Qingwen Li
Summary: This study presents a novel method based on electrochemical shortening and selective adsorption to precisely control the length and distribution of single-walled carbon nanotubes (SWCNTs). Experimental results reveal that SWCNTs with smaller diameters are more prone to truncation, resulting in shorter lengths. Remarkably, a length-dependent fluorescence enhancement was observed when the length of semiconducting-SWCNTs (s-SWCNTs) was reduced to below 120 nm. This research lays the foundation for further exploration of length-dependent properties and applications of s-SWCNTs.
Article
Chemistry, Multidisciplinary
Anatolii Kashchuk, Oleksandr Perederiy, Chiara Caldini, Lucia Gardini, Francesco Saverio Pavone, Anatoliy M. Negriyko, Marco Capitanio
Summary: Particle localization plays a crucial role in advanced biological techniques. This paper presents a universal method based on local gradient calculation, which provides faster and more accurate particle localization under high noise conditions, and can be applied in nanomechanical measurements and microscopy imaging.
Article
Chemistry, Multidisciplinary
Xiaochen Tan, Kevin Welsher
Summary: A new method is introduced to track individual nanoparticles in real-time and measure their protein coronas, revealing that the number of proteins in the dynamic in situ protein corona is twice that of the ex situ measured hard protein corona.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Henok Getachew Girma, Kwang Hun Park, Dongseob Ji, Yejin Kim, Hye Min Lee, Seungju Jeon, Seo-Hyun Jung, Jin Young Kim, Yong-Young Noh, Bogyu Lim
Summary: Although commercialization of semiconducting single-walled carbon nanotube (sc-SWNT) sensors for various gases is hindered by fabrication difficulties, a new method has been developed to fabricate sc-SWNT-based hydrogen (H-2) sensors with excellent reproducibility and high sensitivity. By chemically immobilizing sc-SWNTs and decorating with Pd nanoparticles, uniform-density and monolayer sc-SWNT films are achieved. The optimized sc-SWNT sensor shows high room-temperature response and selectivity for H-2 detection.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Physical
Tian Tian, Huan Yin, Luoxi Zhang, Mingkui Zhu, Defu Ma, Feng Shao, Nantao Hu, Zhi Yang, Yafei Zhang, Yanjie Su
Summary: This study investigates the interfacial charge transfer between gas molecules and SWCNTs using the Raman shift of the 2D peak in SWCNTs. An exponential relationship between the 2D peak position and NO2 concentration has been established, providing a simple method to evaluate charge transfer and gas molecule adsorption state.
APPLIED SURFACE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Boris I. Godoy, Nicholas A. Vickers, Sean B. Andersson
Summary: Single Particle Tracking (SPT) is a powerful method for studying biomolecular dynamics in living cells, revealing trajectories of individual particles and motion model parameters. However, existing algorithms assume constant parameters, while this study proposes an estimation algorithm to determine time-varying parameters in systems that switch between different linear models of motion.
Article
Biochemistry & Molecular Biology
Alessandra Antonucci, Melania Reggente, Alice J. Gillen, Charlotte Roullier, Benjamin P. Lambert, Ardemis A. Boghossian
Summary: The internalization of near-infrared optical nanoprobes in photosynthetic microbes has various applications, but the properties of microbial cell walls can significantly affect nanoprobe uptake and cellular response. This study investigates the interaction of optical nanoprobes with different species and their impact on cell viability. It finds a strong dependence of cell integrity, activity, and viability on the surface functionalization of nanoprobes, and differential uptake of nanoprobes within a single filament.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Minfang Zhang, Ying Xu, Mei Yang, Masako Yudasaka, Toshiya Okazaki
Summary: The study compared the effects of BSA and PEG dispersants on the biodistribution and toxicity of oxidized super-growth single-wall carbon nanotubes injected intravenously into mice over a period of three months. Results showed that both dispersants had limited impact on the biodistribution and toxicity of the carbon nanotubes.
Article
Engineering, Environmental
Qianru Wu, Lu Qiu, Lili Zhang, Huaping Liu, Ruixue Ma, Piao Xie, Runluan Liu, Pengxiang Hou, Feng Ding, Chang Liu, Maoshuai He
Summary: A newly developed Co-MgO catalyst was found to enable the growth of specific types of carbon nanotubes by forming stabilized Co nanoparticles at high temperatures. The selective growth was found to be temperature-dependent and correlated with the relative thermodynamic stability of the carbon nanotube nuclei at different temperatures.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Zhi-Gang Wang, Lei Wang, Don C. Lamb, Hua-Jie Chen, Yusi Hu, Hong-Peng Wang, Dai-Wen Pang, Shu-Lin Liu
Summary: Brain diseases pose an increasing threat to human health, while understanding the transport mechanisms of drugs in live brains remains limited. Through tracking the dynamics of WGA in live brains, this study revealed influences of specific-binding molecules and ECS nature on transport dynamics, paving the way for exploring the biophysics of drug transport in live brains and identifying potential new therapeutic applications for brain diseases.
Article
Multidisciplinary Sciences
Sergey Savitskiy, Rudolf Wachtel, Danial Pourjafar-Dehkordi, Hyun-Seo Kang, Vanessa Trauschke, Don C. Lamb, Michael Sattler, Martin Zacharias, Aymelt Itzen
Summary: The proteolysis of Rab32 by Salmonella typhimurium affects various biochemical and structural properties of the GTPase in diverse ways, demonstrating a localized increase in conformational flexibility and a disruption in the interaction with downstream effectors.
Article
Chemistry, Multidisciplinary
Nader Al Danaf, Waldemar Schrimpf, Patrick Hirschle, Don C. Lamb, Zhe Ji, Stefan Wuttke
Summary: The study reveals that in metal-organic framework crystals, the migration of organic linkers through postsynthetic exchange process can lead to the accumulation of missing-linker defects, resulting in a gradient of defects within the crystal. This process successfully incorporates bulky functional groups into MOFs, expanding their capability to host complex guest species.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Optics
Lea Krafft, Elena Gofas-Salas, Yann Lai-Tim, Michel Paques, Laurent Mugnier, Olivier Thouvenin, Pedro Mece, Serge Meimon
Summary: The new imaging modality presented in the study offers a range of image contrast from full-field to confocal contrast, depending on the pattern size, providing various trade-offs in terms of contrast and acquisition speed. This allows users to choose the most efficient system for a specific clinical application.
Article
Biophysics
Maria Hoyer, Alvaro H. Crevenna, Jose Rafael Cabral Correia, Andrea G. Quezada, Don C. Lamb
Summary: This study used zero-mode waveguides to directly observe the dynamic nucleation process of actin filaments mediated by gelsolin. The stability of actin dimers determines whether elongation occurs and a conformational change in actin associated with filament formation can prevent elongation.
BIOPHYSICAL JOURNAL
(2022)
Article
Multidisciplinary Sciences
Jack P. K. Bravo, Kira Bartnik, Luca Venditti, Julia Acker, Emma H. Gail, Alice Colyer, Chen Davidovich, Don C. Lamb, Roman Tuma, Antonio N. Calabrese, Alexander Borodavka
Summary: It was found that NSP2 autoregulates its chaperone activity through its C-terminal region, promoting RNA-RNA interactions. Additionally, structural proteomics data revealed that the CTR does not directly interact with RNA, but accelerates RNA release from NSP2.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Physics, Multidisciplinary
P. K. Messer, A-K Henss, D. C. Lamb, J. Wintterlin
Summary: This article introduces a multiresolution wavelet particle detection algorithm for tracking and localizing adsorbed atoms and molecules in high-speed scanning tunneling microscopy data. The algorithm can accurately detect particles and obtain extended trajectories by linking particle positions, allowing for the investigation of the motion of single atoms on a surface.
NEW JOURNAL OF PHYSICS
(2022)
Article
Biochemical Research Methods
Viacheslav Mazlin, Olivier Thouvenin, Samer Alhaddad, Martine Boccara, Claude Boccara
Summary: Label-free optical tomography method, full-field optical transmission tomography (FF-OTT), has been developed to reveal intracellular structures and metabolic dynamics. FF-OTT utilizes a common path interferometer and modulates the phase of scattered light by changing the position of the focus, resulting in optical sectioning. Imaging of single-cell diatoms and ex vivo biological samples demonstrates the effectiveness of FF-OTT.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Chemistry, Multidisciplinary
Christin Kirst, Fabian Knechtel, Manuel Gensler, David Fischermeier, Jens Petersen, Nader A. Danaf, Jonathan Tietze, Armin Wedel, Don C. Lamb, Roland Mitric, Konstantin Karaghiosoff
Summary: This study presents new multidentate phosphine oxide ligands and their respective ZnCl2 complexes that exhibit interesting luminescent properties, particularly intense blue luminescence in the crystalline state. The research suggests that the conformation of the molecule, whether restricted by the crystal environment, aggregation, or the steric fixation by the coordinating central atom, can block nonradiative relaxation from the excited state to the ground state. These findings may provide a general mechanism to explain the luminescence properties of organic phosphine oxides.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Frank Mieskes, Evelyn Ploetz, Fabian Wehnekamp, Virgile Rat, Don C. Lamb
Summary: Feedback-based single-particle tracking is a powerful technique for studying particle behavior with high spatiotemporal resolution. This study demonstrates the new modalities available when performing 3D orbital tracking with a second detection channel, including dual-color tracking, tracking interacting particles, and monitoring spectral signature.
Article
Biochemical Research Methods
Salvatore Azzollini, Tual Monfort, Olivier Thouvenin, Kate Grieve
Summary: Recent advances in optical imaging techniques, including dynamic optical coherence tomography (D-OCT) methods, have enabled label-free live optical imaging of dynamic cellular and subcellular features. These techniques analyze the temporal fluctuations of an optical signal associated with intracellular organelle movements to obtain a metric representing cell motility and metabolic state. They allow visualization of cells in compact, static environments and evaluation of their physiology. This review compares various dynamic OCT techniques, discusses current applications, and explores future prospects for the field.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Biochemical Research Methods
Tual Monfort, Salvatore Azzollini, Tasnim Ben Yacoub, Isabelle Audo, Sacha Reichman, Kate Grieve, Olivier Thouvenin
Summary: Dynamic full-field optical coherence tomography (D-FFOCT) is a valuable imaging technique that can capture subcellular biological structures and metabolic activity in complex 3D samples in real time without the need for labeling or invasion. However, DFFOCT often encounters fringe artifacts near reflective surfaces and is highly sensitive to vibrations. In this study, we propose a new configuration called interface Self-Referenced (iSR) D-FFOCT, which uses the sample coverslip as a defocused reference arm, effectively eliminating the aforementioned artifacts. We demonstrate the imaging capability of iSR D-FFOCT on 2D fibroblast cell cultures, which are known for their flatness.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Jules Scholler, Diana Mandache, Marie Christine Mathieu, Aicha Ben Lakhdar, Marie Darche, Tual Monfort, Claude Boccara, Jean-Christophe Olivo-Marin, Kate Grieve, Vannary Meas-Yedid, Emilie Benoit a la Guillaume, Olivier Thouvenin
Summary: Machine learning is employed to interpret D-FF-OCT images of breast surgical specimens, achieving a high accuracy rate above 88% at the image level and above 96% at the specimen level. This demonstrates the potential of D-FF-OCT coupled with machine learning to provide a rapid and accurate histopathology diagnosis.
JOURNAL OF MEDICAL IMAGING
(2023)
Article
Neurosciences
Martin Carbo-Tano, Mathilde Lapoix, Xinyu Jia, Olivier Thouvenin, Marco Pascucci, Francois Auclair, Feng B. Quan, Shahad Albadri, Vernie Aguda, Younes Farouj, Elizabeth M. C. Hillman, Ruben Portugues, Filippo Del Bene, Tod R. Thiele, Rejean Dubuc, Claire Wyart
Summary: Researchers investigate the mesencephalic locomotor region in larval zebrafish and its role in triggering forward locomotion by activating specific sets of hindbrain V2a reticulospinal neurons.
NATURE NEUROSCIENCE
(2023)
Article
Biology
Olivier Thouvenin, Yasmine Cantaut-Belarif, Ludovic Keiser, Francois Gallaire, Claire Wyart
Summary: The circulation of cerebrospinal fluid (CSF) is crucial in development, with recent research on zebrafish embryos showing bidirectional flow in the spinal cord. By automating the quantification of CSF velocity, researchers have been able to study genetic mutants and cilia dynamics to understand the impact on CSF flow. This approach can be extended to study diverse biological solutions and model flow dynamics.
