Article
Chemistry, Analytical
Zhihao Feng, Xiaoyi Li, Changxiang Fang, Junying Li, Rongfei Wang, Weihua Hu
Summary: Oblique-incidence reflectivity difference (OIRD) is a newly established method with great potential for real-time and label-free immunoassay. This study developed a label-free OIRD immunoassay biochip with improved sensitivity by using a standard glass slide coated with polystyrene (PS) microsphere monolayer as the substrate. The immunoassay sensitivity of this biochip was greatly improved compared to the conventional biochip on glass slide, and the improvement was attributed to the high capture efficiency and enhanced intrinsic OIRD sensitivity of the porous PS monolayer. This work provides valuable insights for the development of high-performance label-free OIRD bio-detection devices.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Pharmacology & Pharmacy
Michael D. Scholle, Patrick T. O'Kane, Sandra Dib, Zachary A. Gurard-Levin
Summary: This study describes a duplexed biochemical assay that can efficiently screen potential drugs against COVID-19. The results provide insights into the structural features required for inhibiting the activity of 3CLpro and guide the development of optimized antiviral compounds.
ANTIVIRAL RESEARCH
(2022)
Article
Chemistry, Analytical
Yueli Zhao, Weihua Fan, Anwei Liu, Shihua Pan, Chongxiao Xu, Hailun Peng, Bingling Yin, Xiaodong Wang, Jianhua Dong, Zhiguo Pan
Summary: Heatstroke is a life-threatening injury that can lead to central nervous system dysfunction and severe multiple organ failure syndrome. Cell-cell adhesion and cell permeability are important factors in characterizing heatstroke. A biochip-based electrical model was proposed and applied to investigate the process of heatstroke. The results suggest that the model can be used for monitoring biological processes and other biomedical applications.
Article
Chemistry, Multidisciplinary
Ningyu Wu, Min Wang, Zulipiya Shadike, Zhe Hu, Yanhong Hu, Yue Gao
Summary: Anode-free lithium batteries with high-initial-efficiency, long-cycle-life, and long-calendar-life have been achieved by using an organic Li salt monolayer bonded on Cu. The functional salt results in the formation of a LiF-rich interphase on Cu and guides Li nucleation and growth, reducing Li loss and suppressing galvanic Li corrosion and Li-electrolyte reactions. The activated cell retains 95.2% capacity after 600 cycles and shows no performance damage during calendar aging.
Article
Chemistry, Analytical
Hessamaddin Sohrabi, Mir Reza Majidi, Karim Asadpour-Zeynali, Alireza Khataee, Ahad Mokhtarzadeh
Summary: This research demonstrates a label-free electrochemical-based genosensing assay for the sensitive detection of the L-fuculokinase genome related to Haemophilus influenzae (H. influenzae). Multiple electrochemical modifier-tagged agents were effectively used to enhance the electrochemical responses. The designed genosensing bio-platform achieved low detection and quantification limits (LOD and LOQ) of 6.14 fM and 11 fM, respectively, and demonstrated a wide linear range of 0.1 to 1000 pM. The assay showed high selectivity, good performance, and reliable repeatability and reproducibility, making it a promising method for rapidly and quantitatively detecting H. influenzae.
Article
Chemistry, Analytical
Madisson Chabas, Olivier Pible, Jean Armengaud, Beatrice Alpha-Bazin
Summary: To accurately and rapidly identify microorganisms from complex microbiota for clinical diagnostic purposes, an innovative label-free multiplexing method for the identification of microorganisms using tandem mass spectrometry is proposed in this paper. The method involves offline reversed-phase fractionation of individual peptidomes and achieves multiplexing by mixing fractions of staged hydrophobicity. This approach enables the identification of up to 21 microorganisms in a single 60-minute run without the use of labeling reagents, providing new perspectives for high-throughput proteotyping of bacteria in large culturomics projects.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Michael D. Scholle, Cheng Liu, Jerome Deval, Zachary A. Gurard-Levin
Summary: The study presents the first label-free and high-throughput assay for NSP14 ExoN activity, providing a new approach to antiviral drug discovery efforts. By screening small molecules, optimizing the assay method, identifying candidate inhibitors, and conducting counterscreens for selectivity and intercalation, the study prepares the groundwork for further drug screening.
Article
Chemistry, Multidisciplinary
Yuning Wang, Yu Liu, Shouzhi Yang, Jia Yi, Xiaoyu Xu, Kun Zhang, Baohong Liu, Kun Qian
Summary: A multifunctional self-assembled AuNPs array (MSANA)-based LDI-MS platform was developed for direct amino acids analysis and metabolic profiling in patient CSF samples. The platform demonstrated high selectivity and micromolar sensitivity for capturing and analyzing aromatic amino acids. It also exhibited excellent reproducibility (RSD < 10%) compared to the widely used direct matrix spotting approach (RSD < 44%). The platform was successfully used for metabolic profiling of CSF, enabling the discrimination of medulloblastoma patients from non-tumor controls within minutes. Overall, the MSANA-based LDI-MS platform shows potential clinical values for large-scale metabolic diagnostics and pathogenic mechanism study.
Article
Chemistry, Multidisciplinary
Saikat Das, Taishu Sekine, Haruna Mabuchi, Sakiat Hossain, Subhabrata Das, Shun Aoki, Shuntaro Takahashi, Yuichi Negishi
Summary: In this study, two newly synthesized silver cluster-assembled materials (SCAMs) composed of Ag-14 and Ag-12 chalcogenolate cluster cores bridged by acetylenic bispyridine linkers were reported. The linker structures and electrostatic interaction between positively charged SCAMs and negatively charged DNA enable the SCAMs to suppress the high background fluorescence of single-stranded DNA probes with SYBR Green I nucleic acid stain, resulting in a high signal-to-noise ratio for label-free target DNA detection.
CHEMICAL COMMUNICATIONS
(2023)
Review
Chemistry, Multidisciplinary
Juan Wang, Pepijn W. H. Pinkse, Loes Segerink, Jan C. T. Eijkel
Summary: Photonic crystals are materials that can confine and manipulate incident light, and have been extensively studied for the development of optical sensors with high adsorption and enhanced light-matter interaction. They are mainly utilized through five sensing principles, including sensing of refractive index variations, sensing by lattice spacing variations, enhanced fluorescence spectroscopy, surface-enhanced Raman spectroscopy, and configuration transitions.
Article
Chemistry, Multidisciplinary
Qiangqiang Shi, Chengzhou Song, Minglong Chen, Jie Xu, Shaoqiu Zheng, Jiajia Tan, Jialin Zhang, Ning Wang, Jinming Hu, Shiyong Liu
Summary: Polymeric nanoparticles (NPs) are commonly used for drug delivery, and their biological behavior is usually tracked using fluorescence or isotope labeling methods. However, these labeling techniques may affect the properties of NPs and result in inaccurate information. It is therefore crucial to develop label-free techniques for accurately assessing the biological fate of polymeric NPs. In this study, discrete oligourethane amphiphiles (DOAs) were used to create nanorods (NRs) with distinct surface functional groups. These NRs can be quantified without labeling and their surface functional groups were found to significantly impact their biological behavior.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Yuming Jiang, Alexandre Hutton, Caleb W. Cranney, Jesse G. Meyer
Summary: This article introduces an improved direct infusion and ion mobility method for proteome analysis, and proposes a label-free quantification method DISPA-LFQ, which can rapidly identify and quantify proteins in complex mixtures. This technology has important applications in drug screening, biomarker discovery, and clinical analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Saunak Das, Fumitaka Ishiwari, Yoshiaki Shoji, Takanori Fukushima, Michael Zharnikov
Summary: Triptycene-based molecules provide a promising platform for assembly on solid substrates; the versatility of the approach can be significantly increased by adding specific functional groups; the possibility of click reaction and subsequent derivatization reaction is tested and verified on an ethynyl-substituted triptycene monolayer.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Chemistry, Multidisciplinary
Xun Liu, Yu Zhao, Yuzhe Ding, Jianhua Wang, Juewen Liu
Summary: Gold nanoparticles (AuNPs) have unique optical properties and are widely used in biosensors. This study investigated the effect of hairpin DNA structure and sequence on the stabilization of AuNPs and its implications in AuNP-based label-free colorimetric biosensors. The results showed that hairpin DNA with a tail provided optimal protection for AuNPs, and the flexible tail region favored adsorption to AuNPs. The sensitivity of a DNA sensing assay was doubled when using hairpin DNA with a tail compared to tail-free hairpin.
Article
Chemistry, Analytical
Jiehao Guan, Kaiyu He, Sundaram Gunasekaran
Summary: In this study, a highly sensitive and selective electrochemical probe was designed for detecting antibiotics in foods. By modifying the electrode with nanomaterials and DNA nanotechnology, the conductivity and biocompatibility were improved, and the detection sensitivity was enhanced with the use of self-assembled TDN. The results showed that the electrochemical probe was able to detect antibiotics such as ampicillin in a short time, with high detection limit and stability.
Article
Chemistry, Multidisciplinary
Blaise R. Kimmel, Justin A. Modica, Kelly Parker, Vinayak Dravid, Milan Mrksich
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Multidisciplinary Sciences
Alison A. Bayly, Benjamin R. McDonald, Milan Mrksich, Karl A. Scheidt
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Chemistry, Multidisciplinary
Justin A. Modica, Tsatsral Iderzorig, Milan Mrksich
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Biochemistry & Molecular Biology
Sarah E. Anderson, James E. Longbotham, Patrick T. O'Kane, Fatima S. Ugur, Danica Galonic Fujimori, Milan Mrksich
Summary: By using a new assay, the study explored the binding preferences of the PHD1 domain of histone demethylase KDMSA for H3 ligands, revealing tolerance for H3Q5 mutations and a high-affinity interaction with Q5-serotonylated H3. This work demonstrates the value of PI-SAMDI and fluorescence polarization in investigating protein-ligand binding.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Kevin J. Metcalf, Blaise R. Kimmel, Daniel J. Sykora, Justin A. Modica, Kelly A. Parker, Eric Berens, Raymond Dai, Vinayak P. Dravid, Zena Werb, Milan Mrksich
Summary: This study presents a method to synthetically tune protein conjugates for improved cancer therapy. By utilizing a megamolecule approach, the researchers were able to increase cytotoxicity effects on cancer cells by modifying the domain stoichiometry of the conjugates. The results demonstrate that synthetic control of protein domain stoichiometry can enhance the structure-function relationships of protein conjugate therapeutics for cancer treatment.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Anming Yang, Huanyu Tao, Lindsey C. Szymczak, Liang Lin, Junfeng Song, Yi Wang, Silei Bai, Justin Modica, Sheng-You Huang, Milan Mrksich, Xinxin Feng
Summary: Site-specific modification of proteins using enzymatic incorporation of Dha provides a traceless ligation method and enhances peptide labeling efficiency. This approach has important applications in biological research and drug development.
ACS CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Blaise R. Kimmel, Milan Mrksich
Summary: This paper introduces an enzyme building block for assembling megamolecules, which inhibits protein CRABP2 to control protein domains. By conjugating the inhibitor and a fluorescent tag to a backbone, precise control over the placement of protein domains within a single molecule is achieved.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Jennifer Grant, Blaise R. Kimmel, Lindsey C. Szymczak, Juliet Roll, Milan Mrksich
Summary: This paper presents a method that combines a microfluidic device and self-assembled monolayers to calculate the cooperativity in binding of calcium ions to PAD2. The method uses a small amount of enzyme solution and fluidic inputs, and utilizes iSAMDI to analyze the reactions. This work establishes a high-throughput and label-free approach for studying enzyme-ligand interactions and expands the applicability of microfluidics and MALDI imaging mass spectrometry.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Multidisciplinary Sciences
Zeynab Mousavikhamene, Daniel J. Sykora, Milan Mrksich, Neda Bagheri
Summary: This research successfully introduced an algorithm that could distinguish cancer from non-cancer cells in high-resolution single cell images by exploiting structural differences in the cytoskeleton. Overcoming the barrier of insufficient data in machine learning methodologies, the study identified the most discriminatory features to predict cell type accurately with as few as 100 cells per line. Furthermore, normalizing cell shape improved discrimination of cell lines with difficult-to-distinguish phenotypes, demonstrating the generalizability of the algorithm across various tissue origins.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Amit A. Nagarkar, Samuel E. Root, Michael J. Fink, Alexei S. Ten, Brian J. Cafferty, Douglas S. Richardson, Milan Mrksich, George M. Whitesides
Summary: The study demonstrates that digital data can be stored in mixtures of fluorescent dye molecules on a surface, tethered by amide bonds. Individual dyes in the mixture can be distinguished using a microscope equipped with a fluorescence detector, encoding them into binary information. The use of molecular mixtures instead of sequence-defined macromolecules reduces synthesis time and eliminates sequencing requirements.
ACS CENTRAL SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Cesar A. Patino, Prithvijit Mukherjee, Eric J. Berns, Elamar Hakim Moully, Liliana Stan, Milan Mrksich, Horacio D. Espinosa
Summary: In this study, we developed a novel live-cell analysis device that can perform membrane permeabilization and content sampling. The device interfaces with surface-based biosensors and allows for the delivery of biomolecules and multimodal analysis. Optimization of electroporation parameters enables control over the sampled contents, and the impact of cell morphology on sampling is investigated using deep-learning enhanced image analysis.
Article
Biochemistry & Molecular Biology
Kelly A. Parker, Stephanie Ribet, Blaise R. Kimmel, Roberto dos Reis, Milan Mrksich, Vinayak P. Dravid
Summary: Soft electron microscopy is essential for studying the characteristics of macromolecules, using low electron doses to enhance contrast. By performing scanning transmission electron microscopy in a scanning electron microscope, larger structures can be efficiently characterized with high contrast.
Article
Multidisciplinary Sciences
Che-Fan Huang, Cara J. Gottardi, Milan Mrksich
Summary: Phosphorylation plays a crucial role in cellular signaling, and dysregulation of phosphorylation can lead to diseases. However, while most studies focus on kinases, the role of phosphatases is often overlooked. This study demonstrates that basic amino acids adjacent to phosphotyrosines can restrict the activity of tyrosine phosphatases, providing insights into the mechanisms underlying disease-associated mutations and potential treatment strategies.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Adam J. Pluchinsky, Daniel J. Wackelin, Xiongyi Huang, Frances H. Arnold, Milan Mrksich
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Chemistry, Analytical
Sarah E. Anderson, Natalie S. Fahey, Jungsoo Park, Patrick T. O'Kane, Chad A. Mirkin, Milan Mrksic
