Article
Optics
Aleksandr Barulin, Inki Kim
Summary: Hyperlenses offer the potential for bioimaging beyond the diffraction limit. Researchers demonstrate the feasibility of sub-diffraction fluorescence correlation spectroscopy using a spherical gold/silicon multilayered hyperlens. The hyperlens can effectively distinguish nanoscale transient trapping sites in simulated 2D lipid diffusion in cell membranes.
Article
Chemistry, Analytical
Juan Hu, Li-yuan Pan, Yueying Li, Xiaoran Zou, Bing-jie Liu, BingHua Jiang, Chun-yang Zhang
Summary: This study demonstrates the sensitive detection of SIRT1 using a single quantum dot (QD)-based nanosensor, which can be used for enzyme kinetic parameter measurement and SIRT1 inhibitor screening.
Article
Biochemical Research Methods
Miguel Bernabe-Rubio, Minerva Bosch-Fortea, Miguel A. Alonso, Jorge Bernardino de la Serna
Summary: The assembly of the primary cilium in polarized epithelial cells involves the formation of the centrosome-associated membrane patch (CAMP) from the remnant-associated membrane patch (RAMP), from which the ciliary membrane stems. A correlative imaging approach has been developed to quantitatively analyze the lipid collective assembly at the plasma membrane over time and different spatial scales. These findings provide insights into the mechanisms behind the biogenesis of the ciliary membrane in polarized epithelial cells.
Article
Physiology
Hugo McGuire, Rikard Blunck
Summary: This study observed clustering of KcsA channels in lipid bilayer and demonstrated that the clustering was mediated by microdomains induced by the channel in the lipid matrix. The clustering required specific lipid phases and negative spontaneous curvature, which is important for understanding the oligomerization of membrane proteins.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Chemistry, Analytical
Jiseong Park, Yongdeok Ahn, Wonhee John Lee, Siwoo Jin, Sejoo Jeong, Jaeyong Kim, Young-Sam Lee, Jong-Chan Lee, Daeha Seo
Summary: Researchers have developed a method called lipid-MAP that uses nanoparticles, single-molecule localization microscopy, and a hidden Markov model algorithm to analyze the phase heterogeneity of lipid membranes. They were able to detect and characterize small and transient lipid domains in live cells.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Irina Starostina, Yoon-Kwan Jang, Heon-Su Kim, Jung-Soo Suh, Sang-Hyun Ahn, Gyu-Ho Choi, Myungeun Suk, Tae-Jin Kim
Summary: This study reveals that TRPM7-induced Ca2+ influx mainly occurs at the DRM, and chemically induced mechanical perturbations in the cell mechanosensitive apparatus substantially reduce Ca2+ influx through TRPM7, preferably located at the DRM. Such perturbations include the disintegration of lipid rafts, microtubules, or actomyosin filaments; the alteration of actomyosin contractility; and the inhibition of focal adhesion and Src kinases.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Hotaka Kobayashi, Robert H. Singer
Summary: This study reports single-molecule methods to visualize each step of miRNA-mediated gene silencing inside cells. It is found that miRNAs preferentially bind to translated mRNAs and induce translational repression and mRNA decay after binding to mRNAs.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Claudia Matthaeus, Justin W. Taraska
Summary: Caveolae are plasma membrane invaginations with a diameter of 70-100 nm, abundant in specific cell types and characterized by specific lipid binding proteins. They provide support for proteins involved in cell-specific signaling pathways and play an important role in lipid trafficking and metabolism through their ability to detach from the plasma membrane and move through the cytosol.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Jacob J. Kinnun, Haden L. Scott, Rana Ashkar, John Katsaras
Summary: The relationship between cell membrane structure and function is a fundamental principle in biology; neutron scattering techniques can be used to study hydrogen-rich materials such as biomembranes; modeling small-angle neutron scattering data and molecular dynamics simulations can reveal the structure, dynamics, and molecular interactions of biological membranes.
FRONTIERS IN CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
B. Jonas, D. Heinze, E. Schoell, P. Kallert, T. Langer, S. Krehs, A. Widhalm, K. D. Joens, D. Reuter, S. Schumacher, A. Zrenner
Summary: The authors introduce an all-optical nonlinear method to tailor and control the single photon emission. They demonstrate energy tuning and polarization control of the emitted photons through a laser-controlled down-conversion process.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Ming-hao Liu, Chuan-rui Wang, Wen-jing Liu, Qinfeng Xu, Chun-yang Zhang
Summary: In this study, a quantum dot-mediated fluorescence resonance energy transfer biosensor was developed for the amplification-free measurement of TET2. With high sensitivity and homogeneity, this biosensor enables accurate detection of TET2 at the cellular level, offering a new platform for TET2-related medical research and clinical diagnostics.
Article
Chemistry, Analytical
Sujitha Puthukodan, Martina Hofmann, Mario Mairhofer, Hannah Janout, Jonas Schurr, Fabian Hauser, Christoph Naderer, Johannes Preiner, Stephan Winkler, Dmitry Sivun, Jaroslaw Jacak
Summary: Extracellular vesicles (EVs) have immense potential as therapeutic agents and diagnostic tools due to their role in cell-cell communication. This study utilized single-molecule microscopy techniques to comprehensively characterize and measure the uptake of HEK293T cell-derived EVs (eGFP-labeled) in HeLa cells. The analysis revealed that 68% of fluorescently labeled EVs had an average size of 45 nm. 3D colocalization analysis of two-color direct stochastic optical reconstruction microscopy (dSTORM) images showed that 25% of uptaken EVs colocalized with transferrin.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Mahdi Asgari, Dominique Coquillat, Guido Menichetti, Valentina Zannier, Nina Diakonova, Wojciech Knap, Lucia Sorba, Leonardo Viti, Miriam Serena Vitiello
Summary: Low-dimensional nanosystems show promise in manipulating and controlling photons with large sensitivities, and by quantum engineering tailored energy levels of localized electrons can result in efficient quantum sensors. Utilizing few-electron physics, millimeter-wave nanodetectors have been developed to efficiently sense radiation at 0.6 THz with low noise levels, opening up prospects for quantum communications and cryptography.
Article
Physics, Applied
Yao Ding, Zhengyang Bai, Guoxiang Huang, Weibin Li
Summary: We investigate facilitation-induced transparency (FIT) enabled by strong and long-range Rydberg atom interactions between two spatially separated optical channels.
PHYSICAL REVIEW APPLIED
(2023)
Article
Biochemistry & Molecular Biology
Garth L. Nicolson, Gonzalo Ferreira de Mattos
Summary: The evolution of cell membrane structure has progressed from early models placing proteins external to lipid bilayers towards the fluid-mosaic membrane model, revealing a complex and dynamic organization with specialized domains and essential lipid-protein interactions.