期刊
ACS CHEMICAL BIOLOGY
卷 13, 期 9, 页码 2427-2432出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.8b00639
关键词
-
资金
- Cancer Research UK [C309/A11566]
- Cancer Research UK Accelerator Award [C1362/A20263]
APOBEC3B (A3B) deamination activity on ssDNA is considered a contributing factor to tumor heterogeneity and drug resistance in a number of human cancers. Despite its clinical impact, little is known about A3B ssDNA substrate preference. We have used nuclear magnetic resonance to monitor the catalytic turnover of A3B substrates in real-time. This study reports preferred nucleotide sequences for A3B substrates, including optimized 4-mer oligonucleotides, and reveals a breadth of substrate recognition that includes DNA sequences known to be mutated in drug-resistant cancer clones. Our results are consistent with available clinical and structural data and may inform the design of substrate-based A3B inhibitors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据