Article
Chemistry, Multidisciplinary
Sevde Puza, Stefanie Caesar, Chetan Poojari, Michael Jung, Ralf Seemann, Jochen S. Hub, Bianca Schrul, Jean-Baptiste Fleury
Summary: Lipid droplets (LDs) are organelles derived from the endoplasmic reticulum (ER) membrane and play a crucial role in lipid storage. The distribution of proteins between phospholipid bilayer and LD monolayer membranes is determined by the collective properties of protein-intrinsic and lipid-mediated features. In this study, researchers produced a freestanding phospholipid bilayer using microfluidics and observed the insertion of micrometer-sized LDs into the bilayer. They found that the LDs presented a lens shape and there was a diffusion barrier between the LD monolayer and the bilayer. This barrier may influence the partitioning of proteins between the ER membrane and LDs in living cells.
Review
Biochemistry & Molecular Biology
Yingying Song, Jing Gu, Jingya You, Yiyang Tao, Yao Zhang, Lizhuo Wang, Jialin Gao
Summary: Sidt2 is a lysosomal membrane protein that plays a crucial role in maintaining the normal morphology and stability of lysosomes, as well as regulating glucose and lipid metabolism and nucleotide transport.
Review
Biochemistry & Molecular Biology
Johannes Thoma, Bjorn M. Burmann
Summary: Membrane proteins are evolved to reside in the hydrophobic lipid bilayers of cellular membranes, bridging different aqueous compartments separated by the membrane and dynamically interacting with their surrounding lipid environment. This interaction not only stabilizes membrane proteins, but also influences their folding, structure, and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Nicolas Bloemeke, Kevin Meighen-Berger, Manuel Hitzenberger, Nina C. Bach, Marina Parr, Joao Pl Coelho, Dmitrij Frishman, Martin Zacharias, Stephan A. Sieber, Matthias J. Feige
Summary: One-third of the human proteome is composed of membrane proteins, which are susceptible to misfolding and require folding assistance from chaperones. Calnexin, an abundant ER chaperone, interacts with a large number of membrane proteins, including misfolded ones, using its lectin domain and transmembrane domain for recognition and binding. This study reveals the widespread role of calnexin in recognizing client proteins in the lipid bilayer and highlights its importance in supporting membrane protein biogenesis.
Article
Multidisciplinary Sciences
Kang-Cheng Liu, Hudson Pace, Elin Larsson, Shakhawath Hossain, Aleksei Kabedev, Ankita Shukla, Vanessa Jerschabek, Jagan Mohan, Christel A. S. Bergstrom, Marta Bally, Christian Schwieger, Madlen Hubert, Richard Lundmark
Summary: Caveolae, important plasma membrane invaginations, are shaped by the coat protein Cavin1. This study reveals the mechanism of Cavin1 assembly at the membrane interface, involving initial PI(4,5)P-2-dependent membrane adsorption, subsequent partial separation and membrane insertion. This intricate mechanism facilitates membrane curvature generation and dynamic assembly-disassembly of Cavin1 at the membrane.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Rafael Alvarez, Pablo V. Escriba
Summary: GPCR interacts with lipids to bring G proteins together in specific membrane microdomains, enabling efficient signal transduction. The lipid composition and specific protein regions play a crucial role, but the molecular mechanisms underlying the membrane distribution of each G protein isoform are still largely unknown.
Review
Cell Biology
Fubito Nakatsu, Asami Kawasaki
Summary: This article discusses the mechanism of lipid transport within cells, focusing on the role of oxysterol-binding protein (OSBP)-related proteins (ORPs) in lipid transport and their significance in cellular functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Benedict M. Long, Britta Forster, Sacha B. Pulsford, G. Dean Price, Murray R. Badger
Summary: Membraneless organelles containing Rubisco enzyme are common in organisms using CO2 concentrating mechanisms for photosynthetic carbon acquisition. The Rubisco condensates in different organisms, like carboxysomes in cyanobacteria and proteobacteria, and pyrenoids in some algae and hornworts, enhance CO2 fixation compared to free enzyme. Mathematical models suggest that the enhanced function of carboxysomes is due to elevated CO2 levels generated within the organelle via colocalized carbonic anhydrase and inwardly diffusing bicarbonate ions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Leonhard H. Urner, Florian Junge, Francesco Fiorentino, Tarick J. El-Baba, Denis Shutin, Gideon Noelte, Rainer Haag, Carol V. Robinson
Summary: Membrane protein purification is crucial for isolating therapeutic targets. However, the role of detergent structure in this process is not well understood, leading to failed preparations and increased costs. This study evaluates the use of hydrophilic-lipophilic balance (HLB) concept to optimize dendritic oligoglycerol detergents. The findings provide qualitative HLB guidelines and enable investigation of lipid binding strengths and protein oligomerization.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Radoslaw Starosta, Telma C. Santos, Andreia F. Dinis F. de Sousa, Maria Soledade Santos, M. Luisa Corvo, Ana Isabel Tomaz, Rodrigo F. M. de Almeida
Summary: This study investigates the possible role of the cell membrane in the mechanism of action of two ruthenium complexes against human tumor cells. The results show that these complexes increase the permeability of lipid vesicles and alter the biophysical properties of the membranes. Moreover, the effects of the complexes depend on changes in membrane lipid composition.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Multidisciplinary Sciences
Jeong-Su Park, Jun-Hyeong Kim, Won-Chang Soh, Na-Young Kim, Kyung-Sik Lee, Chang-Hyun Kim, Ik-Joo Chung, Sunjae Lee, Hye-Ran Kim, Chang-Duk Jun
Summary: This study reveals the mechanism of TCR release following T cell activation. It shows that T cell activation leads to TCR shedding in microvilli, which involves trogocytosis and enzymatic vesiculation. Unlike TCR internalization, this shedding event increases surface TCR expression and promotes metabolic reprogramming for cell division and survival.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Francesco Spinozzi, Jean-Pierre Alcaraz, Maria Grazia Ortore, Landry Gayet, Aurel Radulescu, Donald K. Martin, Marco Maccarini
Summary: This study provides a detailed characterization of liposomes containing OprF using small-angle neutron scattering (SANS) and a specific model for data analysis. The quantification of crucial structural information is important for the design and therapeutic applications of liposomal delivery systems.
Review
Plant Sciences
Linhui Yu, Chao Zhou, Jilian Fan, John Shanklin, Changcheng Xu
Summary: Lipid remodeling, defined as post-synthetic modifications of membrane lipids, plays a crucial role in regulating cellular membrane properties and functions. Processes affected by lipid remodeling include lipid metabolism, membrane repair, cellular homeostasis, fatty acid trafficking, cellular signaling, and stress tolerance. Recent research focuses on understanding the mechanisms of membrane lipid remodeling, particularly the enzymes involved in modifying major membrane lipids such as phosphatidylcholine and monogalactosyldiacylglycerol.
Article
Cell Biology
Zachary Graber, Desmond Owusu Kwarteng, Shannon M. Lange, Yannis Koukanas, Hady Khalifa, Jean W. Mutambuze, Edgar E. Kooijman
Summary: Diacylglycerol pyrophosphate (DGPP) is an anionic phospholipid formed in response to stress stimuli, and it interacts with proteins and ions in the membrane, potentially affecting membrane properties and dynamics. However, the impact of these interactions on stress response and overall cellular function of DGPP is still unknown.
Review
Biochemistry & Molecular Biology
Tomohiro Kimura, Atsuko K. Kimura, Richard M. Epand
Summary: Plasmalogen, a major phospholipid in cell membranes, exhibits differential molecular characteristics compared to diacyl glycerophospholipid due to its sn-1 vinyl-ether linkage. NMR plays a significant role in studying plasmalogen at the molecular and tissue levels, providing valuable insights into its regulation of biological membrane processes. The shared enzymes in the de novo synthesis pathways of plasmalogen and diacyl glycerophospholipid contribute to a dynamic crosstalk between these lipids, which plays a crucial role in maintaining lipid balance and compensating for defects. However, this counterbalancing mechanism may lead to imbalances and differential effects on membrane processes in diseased states.
PROGRESS IN LIPID RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Sudeshna Manna, Debayan Sarkar, Seergazhi G. Srivatsan
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Multidisciplinary Sciences
Danish Umar, Arundhoti Das, Suman Gupta, Somdeb Chattopadhyay, Debayan Sarkar, Gauri Mirji, Jeet Kalia, Gopalakrishnan Aneeshkumar Arimbasseri, Jeannine Marie Durdik, Satyajit Rath, Anna George, Vineeta Bal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Pharmacology & Pharmacy
Federica Gasparri, Debayan Sarkar, Sarune Bielickaite, Mette Homann Poulsen, Alexander Sebastian Hauser, Stephan Alexander Pless
Summary: This study investigates the impact of missense variants in the inter-subunit interface of P2X2 receptors in the human population. The results show that these mutations may lead to increased ATP affinity in the P2X2 receptor channels, and there is significant energetic coupling between double mutants at the subunit interface.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemical Research Methods
Debayan Sarkar, Satyajit Mishra, Rahul Nisal, Sumita Majhi, Rohit Shrivas, Yashaswi Singh, V. S. Anusree, Jeet Kalia
Summary: Peptide toxins secreted by venomous animals can bind to and modulate the function of mammalian ion channel proteins. These toxins are highly specific to their target ion channels, making them powerful tools for ion channel biology. However, producing fluorescently labeled toxins rich in cysteine residues presents technical challenges. In this study, we evaluate different protein bioconjugation approaches and find that C-terminal bioconjugation via the sortase technology is successful in producing fluorescently labeled toxins.
BIOCONJUGATE CHEMISTRY
(2022)