Article
Oncology
Zoe Valero-Ramon, Carlos Fernandez-Llatas, Gonzalo Collantes, Bernardo Valdivieso, Antonis Billis, Panagiotis Bamidis, Vicente Traver
Summary: In order to support clinical decision-making and performance evaluation, there is a need for innovative tools that make clinical data more accessible. This study developed an exploratory tool using an interactive Process Mining paradigm to present cancer patients' progress over time.
FRONTIERS IN ONCOLOGY
(2023)
Article
Cell Biology
Salima Benbarche, Omar Abdel-Wahab
Summary: A new study introduces a technique to monitor gene expression dynamically and non-invasively by embedding reporters within introns of the parent gene.
NATURE CELL BIOLOGY
(2022)
Article
Transportation Science & Technology
Matteo Scandella, Arnob Ghosh, Michelangelo Bin, Thomas Parisini
Summary: This paper introduces a macroscopic traffic model to study the impact of rational driver behavior on urban traffic congestion. By providing information about red light duration to drivers and adjusting traffic light control strategies, congestion in the network and queue lengths at intersections can be reduced.
TRANSPORTATION RESEARCH PART C-EMERGING TECHNOLOGIES
(2022)
Article
Chemistry, Multidisciplinary
Fatih Gunes, Selim Bayrakli, Abdul Halim Zaim
Summary: This paper investigated how to improve the performance of signalized intersections by designing a traffic-sensitive signal control system and comparing its effectiveness with other adaptive control systems. The results showed that well-planned and correctly chosen techniques can better regulate traffic density and queuing.
APPLIED SCIENCES-BASEL
(2021)
Article
Engineering, Mechanical
Wenbo Huang, Torgeir Moan
Summary: This paper presents an analytical method for predicting traffic loads and load effects of long span bridges using the Poisson model and normal distribution. The method idealizes traffic states as spatially stationary stochastic fields and models the spatial distribution of vehicles using a Poisson point field. The results show that this analytical method is simple, accurate, and comparable to popular microsimulation methods. It can be easily used to develop a design code for long span bridges and combine traffic loads with other loadings for floating bridges.
PROBABILISTIC ENGINEERING MECHANICS
(2022)
Article
Biochemistry & Molecular Biology
Pernille Sos Hovgaard Jensen, Maja Johansen, Lasse K. Bak, Lars Juhl Jensen, Christina Kjaer
Summary: Study finds that gene expression analysis may be influenced by pre-analytical factors, but the collection time, tissue size, collection method, and RNA isolation method do not significantly affect the yield and integrity of brain-derived RNA, with potential practical and financial implications for clinical genomic departments and other laboratory settings.
NEUROCHEMICAL RESEARCH
(2021)
Article
Chemistry, Analytical
Nuria Fontanals, Eva Pocurull, Rosa Montes, Iria Gonzalez-Marino, Sergio Santana-Viera, Manuel Miro, Andreu Rico, Sara Rodriguez-Mozaz, Francesc Borrull, Jose Benito Quintana, Rosa Maria Marce
Summary: This study describes an analytical method based on solid-phase extraction and liquid chromatography coupled to tandem mass spectrometry to monitor the occurrence of a group of seventeen pharmaceuticals in wastewater. The results showed that all studied pharmaceuticals were found in all samples, with concentrations ranging from non-detectable to 10,393 ng/L, and the highest concentrations were for tramadol. Furthermore, the study also found that the pattern of pharmaceutical use was generally similar in all cities monitored.
MICROCHEMICAL JOURNAL
(2023)
Review
Materials Science, Biomaterials
Chang Yang, Yu Jiang, Sai Heng Hao, Xing Yi Yan, De Fei Hong, Hua Naranmandura
Summary: This review comprehensively discusses the importance and development of aptamer-drug conjugates in targeted drug delivery, as well as the potential application of artificial intelligence techniques in aptamer design.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Review
Clinical Neurology
Stine N. Hansen, Anja Holm, Sakari Kauppinen, Henrik Klitgaard
Summary: This article discusses new strategies for epilepsy treatment using RNA-based drugs, including interference with ncRNAs and mRNAs, and their potential applications in different patient populations.
Article
Biotechnology & Applied Microbiology
Long Zhang, Chaofeng Mu, Tinghong Zhang, Dejun Yang, Chenou Wang, Qiong Chen, Lin Tang, Luhui Fan, Cong Liu, Jianliang Shen, Huaqiong Li
Summary: By assembling RNase resistant RNA nanoparticles (NPs) based on the 3WJ structure from Phi29 DNA packaging motor, we successfully developed RNA NPs equipped with epidermal growth factor receptor (EGFR) targeting aptamer and XBP1 siRNA, which could deplete XBP1 expression, suppress tumor growth, sensitize TNBC to chemotherapy, and inhibit angiogenesis in vivo, demonstrating the potential of RNA NPs as an effective platform for siRNA delivery and therapy for chemotherapy-resistant TNBC.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Aowen Liu, Ming Cheng, Yixuan Zhou, Pan Deng
Summary: Oligonucleotides (OGNs) are a relatively new modality that provides unique opportunities for expanding therapeutic targets. Liquid chromatography-mass spectrometry (LC-MS) is now becoming the preferred method for bioanalysis of OGNs. While ion pairing reversed-phase liquid chromatography (IP-RPLC) has been widely used, there are technical issues associated with these methods. IP-free methods, such as hydrophilic interaction liquid chromatography (HILIC) and anion-exchange techniques, have emerged as promising approaches. In this review, the state-of-the-art IP-RPLC-MS bioanalytical methods for OGNs and their metabolites published in the past 10 years (2012-2022) are critically reviewed. Recent advances in IP-reagent-free LC-MS bioanalysis methods are discussed. Finally, future opportunities for developing new methods for comprehensive bioanalysis of OGNs are described.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Neurosciences
Wenjing Peng, Firas Kobeissy, Stefania Mondello, Chloe Barsa, Yehia Mechref
Summary: Neurological diseases have a significant impact on millions of people worldwide, and their prevalence is increasing due to global aging. Glycosylation, a post-translational modification, has been found to play a vital role in neurological diseases and can provide novel biomarkers and information for disease pathology. Mass spectrometry is considered the most powerful tool for glycan analysis, and various techniques and strategies have been developed to enhance its identification and quantitation in neurological samples.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Casper F. T. Van der Ven, Mark W. Tibbitt, Joao Conde, Alain van Mil, Jesper Hjortnaes, Pieter A. Doevendans, Joost P. G. Sluijter, Elena Aikawa, Robert S. Langer
Summary: The study developed a drug delivery platform using gold nanoparticles and polymer nanoparticles hydrogel to improve miRNA delivery efficiency. The results demonstrated that AuNP-miRNAs could be linearly released with functionality in vitro, leading to a decrease in Luciferase reporter activity in HEK293 cells and successful tracking in a 3D bioprinted CAVD model. In the mouse model, AuNP-miRNAs mainly cleared via the liver and kidneys, showing potential for in vivo applications.
Review
Chemistry, Medicinal
Danielle de Brito E. Cunha, Ana Beatriz Teixeira Frederico, Tamiris Azamor, Juliana Gil Melgaco, Patricia Cristina da Costa Neves, Ana Paula Dinis Ano Bom, Tatiana Martins Tilli, Sotiris Missailidis
Summary: The depth and versatility of siRNA technologies make them suitable for disease targets that cannot be treated with small molecule drugs or require precise gene turn-off. However, the use of siRNA faces several challenges, which can be overcome by utilizing nanometric delivery carriers. siRNA has shown progress in clinical trials and has become a valuable asset for biopharmaceutical companies.
Article
Engineering, Environmental
Christopher Malefors, Erik Svensson, Mattias Eriksson
Summary: Automated tools for waste quantification can provide a more accurate understanding of food waste. This study evaluated a tool for quantifying plate waste in primary school canteens and found it to be highly accurate. The findings revealed that a minority of students accounted for a majority of plate waste, highlighting the importance of targeted interventions to reduce waste.
RESOURCES CONSERVATION AND RECYCLING
(2024)
Article
Chemistry, Organic
Fabienne Seyfert, Mathis Mitha, Hans-Achim Wagenknecht
Summary: The study presents a new strong reducing catalyst for the photoredox catalytic addition of alcohols to alkyl olefins, allowing non-activated substrates to undergo Markovnikov addition. This method also demonstrates tolerance towards various functional groups.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Sara Muller, Felix Manger, Lorenz Graf von Reventlow, Alexander Colsmann, Hans-Achim Wagenknecht
Summary: Supramolecular chemistry enables the construction of complex molecular architectures and the design of collective photophysical properties. Using DNA as a template for supramolecular structures offers precise control of chromophore position. Interaction studies between fullerenes and DNA show potential for advancements in optoelectronic applications.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
David Rombach, Bernhard Birenheide, Hans-Achim Wagenknecht
Summary: Properly activated sulfur hexafluoride can serve as a valuable pentafluorosulfanylation reagent under photoredox catalysis, enabling the synthesis of oxygen-containing heterocycles in a single step from substituted alkenes. This advanced protocol, supported by theoretical calculations, not only provides insights into the pentafluorosulfanylation process and carbon-carbon bond formation, but also significantly expands the scope of valuable building blocks for medicinal and material chemistry.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Review
Chemistry, Multidisciplinary
Jonas Groner, Achim Goepferich, Miriam Breunig
Summary: Atherosclerosis, a chronic inflammatory disease, remains the leading cause of death in developed countries. Current cardiovascular drugs fail to address the root cause of the disease. Recent studies suggest that nanoparticulate carriers can enhance the bioavailability of statins and thiazolidinediones in atherosclerotic plaque, potentially improving their therapeutic effects.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Biochemistry & Molecular Biology
Hans-Achim Wagenknecht
Summary: Local DNA photodamaging by light is well-studied, while the mechanisms of remote DNA photodamage are only partially understood. Remote photodamage involves pathways to remote oxidatively generated DNA damage and DNA-mediated energy transport, providing an important extension to our understanding of light-induced DNA damage.
Article
Chemistry, Organic
Fabian Weick, Desiree Steuernagel, Arina Belov, Hans-Achim Wagenknecht
Article
Chemistry, Medicinal
Raphael Mietzner, Ramona Pawlak, Ernst R. Tamm, Achim Goepferich, Rudolf Fuchshofer, Miriam Breunig
Summary: The loss of Schlemm's canal cell function may be the main cause of primary open-angle glaucoma, and Angpt-1 mimetic nanoparticles could potentially serve as a therapeutic option for restoring this function.
Review
Chemistry, Organic
David Rombach, Hans-Achim Wagenknecht
Summary: This review summarizes recent efforts in the photoactivation of inert sulfur hexafluoride and provides insights into the photochemical mechanisms of switching between fluorination and pentafluoro-sulfanylation reactions of organic substrates.
SYNTHESIS-STUTTGART
(2022)
Article
Biochemical Research Methods
Julian Gebhard, Lara Hirsch, Christian Schwechheimer, Hans-Achim Wagenknecht
Summary: Fluorescent DNA probes were prepared using a modular approach and a click post-synthetic modification strategy. The probes showed high fluorescence light-up effect and brightness. Additionally, electron transfer and energy transfer processes were utilized to further enhance the fluorescence readout.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Desiree Steuernagel, Hans-Achim Wagenknecht
Summary: Acetals and ketals are important protecting groups for carbonyl compounds. A new method for acetalization and ketalization using photoredox catalysis has been developed. The method utilizes a biscyanolated perylene bisimide as an electron-poor photocatalyst and green light (525 nm LED). Silylenolethers derived from aldehydes react efficiently to give acetals with good to excellent yields, and the method shows a broad substrate range and high functional group tolerance. While aldehydes can be directly converted into their respective acetals, ketones must first be converted into silylenolethers before ketalization. The method does not require the use of acids or photoacids, additives or H-atom transfer reagents, expanding the substrate scope and repertoire of photoredox catalysis in carbonyl chemistry.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Philipp Geng, Eileen List, Franziska Ronicke, Hans-Achim Wagenknecht
Summary: Two green fluorescent tetrazine-modified cyanine-styryl dyes were synthesized for bioorthogonal labelling of DNA. The fluorescence of these dyes can be released by their interaction with DNA and through the conjugated tetrazine as quencher moiety. The dyes can penetrate cells at low concentrations and undergo fluorogenic reactions with BCN-modified DNA in living cells. The two-factor fluorescence release significantly improves the signal-to-noise ratio, eliminating the need for washing procedures prior to cell imaging.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Physical
Desiree Steuernagel, David Rombach, Daniel Sack, Martin Koos, Burkhard Luy, Hans-Achim Wagenknecht
Summary: A new method was developed to activate CCl3F using photoredox catalysis and functionalize alkenes with the CCl2F group. N-phenylphenothiazine was used as a highly reducing organophotocatalyst. This approach combines the efficient disposal of ozone depleting substances and greenhouse gases with the synthesis of valuable organic compounds, complements the concept of atom transfer radical addition, and provides direct synthetic access to the CCl2F group as a structural motif. The broad substrate scope and multifunctionalized product class enable various subsequent transformations.
Editorial Material
Chemistry, Multidisciplinary
Desiree Steuernagel, Hans-Achim Wagenknecht
Summary: Desiree Steuernagel and Hans-Achim Wagenknecht from the Karlsruhe Institute of Technology are featured on the cover of this issue. The image showcases a traffic roundabout, symbolizing the photocatalytic cycle for the conversion of aldehydes and silylenolethers into their acetals. Read the full article at 10.1002/chem.202203767.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Hulya Ucar, Hans-Achim Wagenknecht
Summary: Cyanolated distyrylbenzene conjugated to 2'-deoxyuridine is a new building block for supramolecular DNA architectures that combine aggregation-induced emission and sequence-selective binding. Cyanolated distyrylbenzenes occupy a high number of binding sites on the DNA template. Light can be collected and transferred to terminal Atto dyes in this assembly. Mixed DNA architectures with perylene can be programmed by the sequence of the DNA template.
CHEMICAL COMMUNICATIONS
(2022)
