期刊
ACS CHEMICAL BIOLOGY
卷 7, 期 12, 页码 1935-1949出版社
AMER CHEMICAL SOC
DOI: 10.1021/cb300449t
关键词
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资金
- Action Thematique Incitative Genopole
- ANR Blanche TRANSFOLDPROT [BLAN08-1_339991]
Proteins subjected to an electric field and forced to pass through a nanopore induce blockades of ionic current that depend on the protein and nanopore characteristics and interactions between them. Recent advances in the analysis of these blockades have highlighted a variety of phenomena that can be used to study protein translocation and protein folding, to probe single-molecule catalytic reactions in order to obtain kinetic and thermodynamic information, and to detect protein-antibody complexes, proteins with DNA and RNA aptamers, and protein-pore interactions. Nanopore design is now well controlled, allowing the development of future biotechnologies and medicine applications.
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