期刊
ACS CHEMICAL BIOLOGY
卷 6, 期 1, 页码 61-74出版社
AMER CHEMICAL SOC
DOI: 10.1021/cb100285n
关键词
Directed evolution; Mammalian expression system; Post-translational modification; Protein engineering; Retrovirus; Screening
资金
- Fonds der Chemischen Industrie
- Deutsche Forschungsgemeinschaft (DEG) [UR 215/1-1]
The display and analysis of proteins expressed on biological surfaces has become an attractive tool for the study of molecular interactions In enzymology, protein engineering, and high-throughput screening. Among the growing number of established display systems, retroviruses offer a unique and fully mammalian platform for the expression of correctly folded and post-translationally modified proteins In the context of cell plasma membrane-derived particles. This Is of special interest for therapeutic applications such as gene therapy and vaccine development and also offers advantages for the engineering of mammalian proteins toward customized binding affinities and catalytic activities. This review critically summarizes the basic concepts and applications of retroviral display and analyses its benefits in comparison to other display techniques.
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