期刊
ACS CHEMICAL BIOLOGY
卷 3, 期 10, 页码 625-634出版社
AMER CHEMICAL SOC
DOI: 10.1021/cb800143w
关键词
-
资金
- Wellcome Trust [071463]
- Wellcome Trust International [076254/Z/04/Z]
Glycosylphosphatidylinositol (GPI)-anchored proteins are abundant in the protozoan parasite Tryponosoma brucei, the causative agent of African sleeping sickness in humans and the related disease Nagana in cattle, and disruption of GPI biosynthesis is genetically and chemically validated as a drug target. Here, we examine the ability of enzymes of the trypanosomal GPI biosynthetic pathway to recognize and process a series of synthetic dimannosyl-glucos-aminylphosphatidylinositol analogues containing systematic modifications on the mannose residues. The data reveal which portions of the natural substrate are important for recognition, explain why mannosylation occurs prior to inositol acylation in the trypanosomal pathway, and identify the first inhibitor of the third alpha-mannosyltransferase of the GPI biosynthetic pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据