期刊
ACS APPLIED MATERIALS & INTERFACES
卷 6, 期 19, 页码 17296-17305出版社
AMER CHEMICAL SOC
DOI: 10.1021/am505469n
关键词
versatile matrix; redox enzyme; gold nanoparticles; hydrogen peroxide biosensors; self-assembled monolayers
资金
- National Natural Science Foundation of China [21275113, 21075096, 21375041]
A versatile matrix was fabricated and utilized as a universal interface for the construction of enzyme-based biosensors. This matrix was formed on the gold electrode via combining self-assembled monolayer of 2,3-dimercaptosuccinic acid with gold nanoparticles. Gold nanoparticles were electrochemically deposited. Electrochemistry of three redox enzymes (catalase, glucose oxidase, and horseradish peroxidase) was investigated on such a matrix. The electrocatalytic monitoring of hydrogen peroxide and glucose was conducted on this matrix after being coated with those enzymes. On them the monitoring of hydrogen peroxide and glucose shows rapid response times, wide linear working ranges, low detection limits, and high enzymatic affinities. This matrix is thus a versatile and suitable platform to develop highly sensitive enzyme-based biosensors.
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