期刊
ACS APPLIED MATERIALS & INTERFACES
卷 6, 期 22, 页码 20372-20377出版社
AMER CHEMICAL SOC
DOI: 10.1021/am505913d
关键词
prostate-specific antigen (PSA); rolling circle amplification (RCA); DNA origami; DNA nanostructures; signal amplification
资金
- National Basic Research Program of China (973 program) [2013CB932802]
- National Nature Science Foundation of China [21305088, 51073173]
- Shanghai Science and Technology Development Funds [13QB1402100]
- National High Technology Research and Development Program of China [2013AA032203]
Prostate-specific antigen (PSA) is one of the most important biomarkers for the early diagnosis and prognosis of prostate cancer. Although many efforts have been made to achieve significant progress for the detection of PSA, challenges including relative low sensitivity, complicated operation, sophisticated instruments, and high cost remain unsolved. Here, we have developed a strategy combining rolling circle amplification (RCA)-based DNA belts and magnetic bead-based enzyme-linked immunosorbent assay (ELISA) for the highly sensitive and specific detection of PSA. At first, a 96-base circular DNA template was designed and prepared for the following RCA. Single stranded DNA (ssDNA) products from RCA were used as scaffold strand for DNA origami, which was hybridized with three staple strands of DNA. The resulting DNA belts were conjugated with multiple enzymes for signal amplification and then employed to magnetic bead based ELISA for PSA detection. Through our strategy, as low as 50 aM of PSA can be detected with excellent specificity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据