期刊
ACS APPLIED MATERIALS & INTERFACES
卷 5, 期 23, 页码 12249-12253出版社
AMER CHEMICAL SOC
DOI: 10.1021/am404314p
关键词
G-quadruplex; iridium(III) complex; label-free; AP endonuclease; luminescent
资金
- Hong Kong Baptist University [FRG2/12-13/021]
- Centre for Cancer and Inflammation Research
- School of Chinese Medicine (CCIR-SCM, HKBU)
- Health and Medical Research Fund [HMRF/11101212, HMRF/13121482]
- Research Grants Council [HKBU/201811, HKBU/204612, HKBU/201913]
- French National Research Agency/Research Grants Council Joint Research Scheme [103/2012/A3, 001/2012/A]
- Science and Technology Development Fund, Macao SAR [103/2012/A3, 001/2012/A]
- University of Macau [MYRG091(Y2-L2)-ICMS12-LCH, MYRG121(Y2-L2)-ICMS12-LCH]
We report herein the synthesis and application of a novel G-quadruplex-selective luminescent iridium(III) complex [Ir(ppy)(2)(bcp)](+) (where ppy = 2-phenylpyridine and bcp = 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline) for the sensitive detection of apurinic/apyrimidinic (AP) endonuclease activity. Using endonuclease IV (Endo IV) as a model enzyme, a duplex DNA substrate containing a G-quadruplex-forming sequence is cleaved by Endo IV at the abasic site. This releases the G-quadruplex sequence, which folds into a G-quadruplex and is recognised by the G-quadruplex-selective iridium(III) complex with an enhanced luminescence response. The assay achieved high sensitivity and selectivity for Endo IV over other tested enzymes.
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