期刊
ACS APPLIED MATERIALS & INTERFACES
卷 4, 期 12, 页码 6450-6453出版社
AMER CHEMICAL SOC
DOI: 10.1021/am302268t
关键词
graphene oxide; hybridization chain reaction; MicroRNA; fluorescence sensor; enzyme-free; fluorescence imaging
资金
- National Natural Science Foundation of China [20925519, 91127035, 21105020]
- Natural Science Foundation of Hebei Province [B2011201175, B2012201108]
A new enzyme-free signal amplification-based assay for microRNA (miRNA) detection is developed by using hybridization chain reaction (HCR) coupled with a graphene oxide (GO) surface-anchored fluorescence signal readout pathway. MiRNAs can efficiently initiate HCR between two species of fluorescent hairpin probes. After HCR, both of the excess hairpin probes and the HCR products will be anchored on the GO surface. The fluorescence of the hairpin probes can be completely quenched by GO, whereas the HCR products maintain strong fluorescence. Therefore, integrating HCR strategy for signal amplification with selective fluorescence quenching effects of GO provides a versatile miRNA assay.
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