Article
Biochemistry & Molecular Biology
Divya Seth, Colin T. Stomberski, Precious J. McLaughlin, Richard T. Premont, Kathleen Lundberg, Jonathan S. Stamler
Summary: The aim of this study was to investigate the interactions between nitric oxide synthase (NOS) isoforms and proteins, as well as the mechanisms by which they mediate S-nitrosylation. The results showed that each NOS isoform interacts with and S-nitrosylates many proteins, but a large proportion of S-nitrosylated proteins are not associated with NOS. Additionally, the interactomes and S-nitrosylomes of individual NOS isoforms are largely unique, indicating specific roles for each isoform in S-nitrosylation.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Agriculture, Multidisciplinary
Yulin Zhao, Haoyan Huang, Ning Lv, Chunyan Huang, Huili Chen, Han Xing, Chaorui Guo, Ning Li, Di Zhao, Xijing Chen, Yongjie Zhang
Summary: In this study, the thiol-GSH conjugates of GP, termed M532-1 and M532-2, were identified for the first time. The catalytic activities of GSTs were investigated both in vitro and in vivo. It was found that GSTA1-1 and GSTA4-4 exhibited high activity in the formation of both thiol-GSH conjugates, with GSTA4-4 specifically catalyzing M532-2 formation in vitro. The study demonstrated the inactivation of GP by GSTs and suggested that interindividual variability of GSTs may be a risk factor for susceptibility to GE-induced hepatotoxicity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Stanislava Vrankova, Zuzana Galandakova, Jakub Benko, Martina Cebova, Igor Riecansky, Olga Pechanova
Summary: The results of the study indicate that 10 weeks of social isolation led to a significant decrease in PPI, accompanied by a decrease in NOS activity in the cerebral cortex and the cerebellum, an increase in iNOS in the hippocampus, and an increase in CD concentration in cortex homogenate. On the other hand, 29 weeks of social isolation had an opposite effect on NOS activity, with an increase in the cerebral cortex and the cerebellum in animals reared in a social environment, accompanied by a decrease in CD concentration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Biochemistry & Molecular Biology
Tiffany M. Russell, Des R. Richardson
Summary: GSTs are versatile proteins that play important roles in detoxification and cellular nitric oxide metabolism. They have the ability to convert organic nitrates into nitric oxide, thereby regulating vasodilation. GSTs are also considered suitable targets for pharmaceutical development, particularly in the field of anti-cancer drugs.
Article
Microbiology
Anna C. Lienkamp, Jan Burnik, Thomas Heine, Eckhard Hofmann, Dirk Tischler
Summary: This study investigated the styrene-specific degradation pathway in Actinobacterium Gordonia rubripertincta CWB2, characterizing two glutathione S-transferases. The enzymes were shown to be crucial for the ability of CWB2 to produce valuable chemical compounds, suggesting potential for biotechnological applications. The research provides insights into both the specific bacterial degradation pathway and the rare field of actinobacterial glutathione metabolism.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemistry & Molecular Biology
Tiffany M. Russell, Des R. Richardson
Summary: Glutathione-S-transferases (GSTs) are isozymes that play important roles in both detoxification and redox stress suppression by conjugating glutathione (GSH) to xenobiotics and interacting with cytotoxic nitric oxide (NO). The interactions between NO and GSTs involve binding and storage of NO as dinitrosyl-dithiol iron complexes (DNICs). GSTP1 can inhibit inducible nitric oxide synthase (iNOS) and suppress c-Jun N-terminal kinase (JNK) and nuclear factor kappa B (NF-kappa B) pathways, leading to decreased apoptosis and modulating NO-mediated ROS generation. These studies highlight the innovative role of GSTs in NO metabolism and their interactions with multiple effector proteins.
Article
Ophthalmology
Tadanobu Yoshikawa, Kenji Obayashi, Kimie Miyata, Keigo Saeki, Nahoko Ogata
Summary: The study revealed a significant association between higher serum ADMA levels and severe glaucoma, which remained significant after adjusting for potential confounders.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Review
Food Science & Technology
Joseph Festa, Mariasole Da Boit, Aamir Hussain, Harprit Singh
Summary: Cardiovascular disease is the leading cause of global death, with endothelial dysfunction being a predictor for most CVD. Polyphenols from sources like wine and grape extracts can help prevent endothelial dysfunction, while anthocyanin flavonoids found in berries also improve vascular function.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Nutrition & Dietetics
Qing Li, Yushi Du, Panyin Xiang, Guanyu Chen, Xiaoxian Qian, Shuangshuang Li, Yihui Mao, Wenhua Ling, Dongliang Wang
Summary: Brussels chicory, a typical vegetable in Mediterranean diets, has been found to stabilize advanced atherosclerotic plaques in the brachiocephalic artery of apoE-deficient mice. This study investigated the mechanism behind this effect and found that Brussels chicory improved oxidative stress, leading to the stabilization of aortic plaques and inhibition of monocyte adhesion.
Article
Nutrition & Dietetics
Agnieszka Zembron-Lacny, Edyta Wawrzyniak-Gramacka, Anna Ksiazek, Aleksandra Zagrodna, Wieslaw Kopec, Malgorzata Slowinska-Lisowska
Summary: Intense physical exercise leads to increased production of reactive oxygen and nitrogen species. This study examined the effects of dipeptide intake combined with exercise on the oxi-antioxidant response. The results showed that dipeptide intake led to a significant increase in the NO/H2O2 ratio after exercise, indicating enhanced antioxidant status. Additionally, dipeptides were found to reduce C-reactive protein levels, suggesting their anti-inflammatory properties. These findings suggest that dipeptides have potential in reducing oxidative stress and inflammation in physically active individuals.
Article
Biochemistry & Molecular Biology
Bright Starling Emerald, Sahar Mohsin, Crystal D'Souza, Annie John, Hussain El-Hasasna, Shreesh Ojha, Haider Raza, Basel Al-Ramadi, Ernest Adeghate
Summary: Nitric oxide is involved in the development of diabetes, with neuronal nitric oxide synthase playing a role in the pancreatic beta cell metabolism. However, nNOS disappears quickly in pancreatic beta cells while increasing in pancreatic nerves during the early stages of diabetes. Oxidative stress, measured by ROS and TBARS, is increased in the early stages of diabetes but decreases over time.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Gianfranco Matrone, Sung Yun Jung, Jong Min Choi, Antrix Jain, Hon-Chiu Eastwood Leung, Kimal Rajapakshe, Cristian Coarfa, Julie Rodor, Martin A. Denvir, Andrew H. Baker, John P. Cooke
Summary: Despite the importance of nitric oxide signaling, the role of inducible nitric oxide synthase (iNos) in nuclear translocation and S-nitrosylation in tissue regeneration has been discovered in this study. Kdm1a S-nitrosylation is shown to be crucial for tailfin regeneration, altering its binding to the CoRest complex and impairing H3K4 demethylase activity, highlighting the importance of this post-translational modification in the repair process.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Amir Asgari, Paul Jurasz
Summary: Megakaryocytes are important members of the hematopoietic system, responsible for regulating vascular homeostasis through platelets. The process of megakaryopoiesis generates mature megakaryocytes which release proplatelets into blood vessels. Nitric oxide (NO) plays a modulatory role in hematopoiesis and hemostasis. This review summarizes the effect of NO and its signaling on megakaryopoiesis and thrombopoiesis under physiological and pathophysiological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Cheng-Ta Wu, Rio L. C. Lin, Pei-Hsun Sung, Feng-Chih Kuo, Hon-Kan Yip, Mel S. Lee
Summary: This study identified the association between NOS polymorphism and ONFH, as well as its relationship with coagulopathy, which may contribute to a higher risk of bilateral ONFH.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Plant Sciences
Yuwei Cao, Leifeng Xu, Hua Xu, Panpan Yang, Guoren He, Yuchao Tang, Xianyu Qi, Meng Song, Jun Ming
Summary: The study identified the LhGST gene in Asiatic hybrid lilies, which may be involved in anthocyanin sequestration and transport in lily tepals. Functional analysis confirmed the role of LhGST in anthocyanin accumulation. Additionally, the promoter of LhGST contains various hormone- and stress-responsive elements, and is regulated by a MYB transcription factor.
PLANT CELL REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
Cristine Schmidt, Daniel Moreira-Goncalves, Mario Santos, Adelino Leite-Moreira, Jose Oliveira
Summary: Exercise training can improve symptoms, quality of life, and cardiac function in HFpEF patients, but its effect on mortality and hospitalization needs further investigation.
HEART FAILURE REVIEWS
(2022)
Review
Endocrinology & Metabolism
Giorjines Boppre, Florencio Diniz-Sousa, Lucas Veras, Jose Oliveira, Helder Fonseca
Summary: Exercise, combined exercise regimens, and interventions starting more than 6 months after bariatric surgery were effective in promoting reductions in body weight, waist circumference, and body mass index. Exercise following bariatric surgery did not appear to favor improvements in body composition.
OBESITY SCIENCE & PRACTICE
(2022)
Correction
Surgery
Florencio Diniz-Sousa, Giorjines Boppre, Lucas Veras, Alba Hernandez-Martinez, Jose Oliveira, Helder Fonseca
Review
Surgery
Florencio Diniz-Sousa, Giorjines Boppre, Lucas Veras, Alba Hernandez-Martinez, Jose Oliveira, Helder Fonseca
Summary: The study aimed to assess the effect of exercise on bone mineral density after bariatric surgery. Systematic search found that exercise had a positive effect on BMD at the total hip, femoral neck, lumbar spine, and 1/3 radius. However, the certainty of evidence was low.
Article
Sport Sciences
Florencio Diniz-Sousa, Tiago Granja, Giorjines Boppre, Lucas Veras, Vitor Devezas, Hugo Santos-Sousa, John Preto, Leandro Machado, Joao Paulo Vilas Boas, Jose Oliveira, Helder Fonseca
Summary: Patients who undergo bariatric surgery have an increased risk of falls. A study found that a supervised multicomponent exercise intervention program can improve balance parameters, reducing falls and associated injuries in these patients.
INTERNATIONAL JOURNAL OF SPORTS MEDICINE
(2022)
Review
Surgery
Giorjines Boppre, Florencio Diniz-Sousa, Lucas Veras, Jose Oliveira, Helder Fonseca
Summary: The study found that exercise can reduce cardiometabolic risk factors after bariatric surgery. Furthermore, aerobic plus resistance training had better effects, and interventions starting after 6 months post-surgery and lasting for more than 12 weeks showed more significant results.
Article
Biology
Suiane Cavalcante, Manuel Teixeira, Ana Duarte, Miriam Ferreira, Maria Simoes, Maria Conceicao, Mariana Costa, Ilda P. Ribeiro, Ana Cristina Goncalves, Jose Oliveira, Fernando Ribeiro
Summary: This study aimed to assess the effect of different durations of exercise sessions on the circulating levels of endothelial progenitor cells (EPCs) and endothelial cells (CECs) among adults with cardiovascular risk factors. The findings showed that a 30-minute multicomponent exercise session significantly increased the circulating number of EPCs, indicating positive effects on vascular health.
Article
Sport Sciences
Lucas Veras, Florencio Diniz-Sousa, Giorjines Boppre, Ana Resende-Coelho, Edgar Moutinho-Ribeiro, Vitor Devezas, Hugo Santos-Sousa, John Preto, Joao Paulo Vilas-Boas, Leandro Machado, Jose Oliveira, Helder Fonseca
Summary: This study developed accelerometry-based equations to predict pGRF and pLR during walking and running, and compared the predicted values with actual values, demonstrating high accuracy of these equations.
EUROPEAN JOURNAL OF SPORT SCIENCE
(2023)
Article
Peripheral Vascular Disease
Susana Lopes, Jose Mesquita-Bastos, Catarina Garcia, Daniela Figueiredo, Jose Oliveira, Guilherme Guimaraes, Linda S. Pescatello, Jorge Polonia, Alberto J. Alves, Fernando Ribeiro
Summary: The response of blood pressure to acute exercise appears to predict the ambulatory blood pressure response to exercise training in patients with resistant hypertension.
HYPERTENSION RESEARCH
(2022)
Article
Multidisciplinary Sciences
Maxime Boy, Valeria Bisio, Lin-Pierre Zhao, Fabien Guidez, Berenice Schell, Emilie Lereclus, Guylaine Henry, Juliette Villemonteix, Fernando Rodrigues-Lima, Katia Gagne, Christelle Retiere, Lise Larcher, Rathana Kim, Emmanuelle Clappier, Marie Sebert, Arsene Mekinian, Olivier Fain, Anne Caignard, Marion Espeli, Karl Balabanian, Antoine Toubert, Pierre Fenaux, Lionel Ades, Nicolas Dulphy
Summary: NK cells in MDS patients with TET2 mutations exhibit phenotypic defects, including increased DNA methylation, reduced expression of KIR, perforin, and TNF-a. Inhibition of TET2 in NK cells from healthy donors decreases their cytotoxicity, indicating its critical role in NK cell function. Treatment with azacytidine enhances KIR and cytolytic protein expression, as well as IFN-gamma production in NK cells from patients.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Lin-Pierre Zhao, Marie Sebert, Arsene Mekinian, Olivier Fain, Marion Espeli, Karl Balabanian, Nicolas Dulphy, Lionel Ades, Pierre Fenaux
Article
Chemistry, Analytical
Lucas Veras, Florencio Diniz-Sousa, Giorjines Boppre, Vitor Devezas, Hugo Santos-Sousa, John Preto, Joao Paulo Vilas-Boas, Leandro Machado, Jose Oliveira, Helder Fonseca
Summary: The purpose of this study was to develop prediction equations for peak ground reaction force (pGRF) and peak loading rate (pLR) in high-impact activities for adults with various body masses. Regression equations were developed using accelerometry data, with body mass and peak acceleration or acceleration rate as predictors. The equations had high accuracy for predicting pGRF, with a coefficient of determination (R-2) of at least 0.83 and a mean absolute percentage error (MAPE) below 14.5%. However, the accuracy of the pLR prediction equations was lower than that of the pGRF equations.
Article
Multidisciplinary Sciences
Adrienne Anginot, Julie Nguyen, Zeina Abou Nader, Vincent Rondeau, Amelie Bonaud, Maria Kalogeraki, Antoine Boutin, Julia P. Lemos, Valeria Bisio, Joyce Koenen, Lea Hanna Doumit Sakr, Amandine Picart, Amelie Coudert, Sylvain Provot, Nicolas Dulphy, Michel Aurrand-Lions, Stephane J. C. Mancini, Gwendal Lazennec, David H. McDermott, Fabien Guidez, Claudine Blin-Wakkach, Philip M. Murphy, Martine Cohen-Solal, Marion Espeli, Matthieu Rouleau, Karl Balabanian
Summary: WHIM Syndrome is a rare immunodeficiency caused by mutations in CXCR4 gene. This study found that 25% of WHIM patients have decreased bone mineral density and bone defects leading to osteoporosis. The researchers also discovered that impaired CXCR4 signaling disrupts cell cycle progression and osteogenic commitment of skeletal stromal/stem cells, resulting in imbalanced bone tissue.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Suiane Cavalcante, Sofia Viamonte, Rui S. Cadilha, Ilda P. Ribeiro, Ana Cristina Goncalves, Joao Sousa-Venancio, Marisol Gouveia, Manuel Teixeira, Mario Santos, Jose Oliveira, Fernando Ribeiro
Summary: This study aims to evaluate the effects of a single exercise bout on the circulating levels of EPCs and CECs in heart failure patients. The results showed that a single exercise bout increased the levels of EPCs but didn't change the levels of CECs. Heart failure patients had lower levels of EPCs compared to the age-matched group, but the exercise bout restored their EPCs levels to a level similar to the age-matched group.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Vincent Jachiet, Laure Ricard, Pierre Hirsch, Florent Malard, Laurent Pascal, Odile Beyne-Rauzy, Pierre Peterlin, Alexandre Thibault Jacques Maria, Norbert Vey, Maud D'Aveni, Marie-Pierre Gourin, Sophie Dimicoli-Salazar, Anne Banos, Stefan Wickenhauser, Louis Terriou, Benoit De Renzis, Eric Durot, Shanti Natarajan-Ame, Anne Vekhoff, Laurent Voillat, Sophie Park, Julien Vinit, Celine Dieval, Azeddine Dellal, Vincent Grobost, Lise Willems, Julien Rossignol, Eric Solary, Olivier Kosmider, Nicolas Dulphy, Lin Pierre Zhao, Lionel Ades, Pierre Fenaux, Olivier Fain, Mohamad Mohty, Beatrice Gaugler, Arsene Mekinian
Summary: Background systemic inflammatory and autoimmune diseases (SIADs) occur in a significant proportion of myelodysplastic syndrome (MDS) patients. The recently identified VEXAS syndrome, associated with somatic mutations in UBA1, is characterized by severe inflammatory conditions and hematological abnormalities, including MDS. However, the mechanisms underlying the association between MDS and SIADs are largely unknown. This study aimed to evaluate myeloid immune cell subsets in MDS patients with and without SIAD and compare them to healthy controls.
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
