期刊
AAPS JOURNAL
卷 12, 期 4, 页码 608-616出版社
SPRINGER
DOI: 10.1208/s12248-010-9224-y
关键词
intracellular peptide; mass spectrometry; MHC-I antigen presentation; peptidome; proteasome
资金
- Sao Paulo State Research Foundation (FAPESP) [04/04933-2, 04/14846, 2010/00828-0]
- Financiadora de Estudos e Projetos (FINEP) [A-03/134]
- Brazilian National Research Council (CNPq) [559698/2009-7]
- Instituto de Investigacao em Imunologia iii-Instituto Nacional de Ciencia e Tecnologia [573879/2008-7]
Cells produce and use peptides in distinctive ways. In the present report, using isotope labeling plus semi-quantitative mass spectrometry, we evaluated the intracellular peptide profile of TAP1/beta 2m(-/-) (transporter associated with antigen-processing 1/beta 2 microglobulin) double-knockout mice and compared it with that of C57BL/6 wild-type animals. Overall, 92 distinctive peptides were identified, and most were shown to have a similar concentration in both mouse strains. However, some peptides showed a modest increase or decrease (similar to 2-fold), whereas a glycine-rich peptide derived from the C-terminal of neurogranin (KGPGPGGPGGAGGARGGAGGGPSGD) showed a substantial increase (6-fold) in TAP1/beta 2m(-/-) mice. Thus, TAP1 and beta 2microglobulin have a small influence on the peptide profile of neuronal tissue, suggesting that the presence of peptides derived from intracellular proteins in neuronal tissue is not associated with antigens of the class I major histocompatibility complex. Therefore, it is possible that these intracellular peptides play a physiological role.
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