Article
Agricultural Engineering
Yu -Chen Liu, Javier Ramiro-Garcia, Lara M. Paulo, Camilla Maria Braguglia, Maria Cristina Gagliano, Vincent O'Flaherty
Summary: The feasibility of psychrophilic anaerobic treatment of LCFA-containing dairy wastewater was evaluated and compared to mesophilic anaerobic treatment in this study. The results showed that LCFA in dairy wastewater could be effectively treated at 15 degrees Celsius, with removal rates exceeding 90%. However, efficient long-term treatment required prior acclimation of the biomass to psychrophilic temperatures. Microbial community analysis revealed the crucial roles of putative syntrophic fatty acid bacteria and Methanocorpusculum in LCFA degradation during both mesophilic and psychrophilic treatments. Additionally, a fungal-bacterial biofilm was found to be important during the psychrophilic treatment. Overall, these findings demonstrate the potential of psychrophilic anaerobic treatment for industrial wastewaters and emphasize the importance of understanding the microbial communities involved in the process.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Michel A. Hoogenkamp, Bernd W. Brandt, Alexa M. G. A. Laheij, Johannes J. de Soet, Wim Crielaard
Summary: This study found that dental unit water systems in the Netherlands are prone to biofilm formation, with microbial load and composition influenced by various factors. Significant effects on microbial load and microbiome were only seen when adhering to disinfection protocols. Analysis of relaxed biofilm samples provides better insight into the presence of opportunistic pathogens compared to traditional methods like heterotrophic plate counts.
Review
Microbiology
Ida Clement Thaarup, Anne Kristine Servais Iversen, Mads Lichtenberg, Thomas Bjarnsholt, Tim Holm Jakobsen
Summary: This review summarizes the knowledge obtained from human clinical samples regarding bacterial biofilm survival strategies in chronic wounds, clarifying the limitations of the current knowledge in this specific topic. While several attributes of biofilms are speculated to contribute to their enhanced persistence in chronic wounds, only some of these attributes have been demonstrated to exist in human wounds.
Review
Biochemistry & Molecular Biology
Yumei Ge, Qingqing Wang
Summary: The occurrence of chronic wounds is a major global health issue. Fungi play a substantial role in these wounds, but their prevalence is often underestimated in clinical treatment. Fungi can cause local infections and lead to multiresistant species, making wounds difficult to heal. Candida albicans yeast and Trichophyton rubrum filamentous fungi are the main types involved in chronic wound infection. This review outlines the latest research on fungi in chronic wounds and addresses challenges and future perspectives in diagnosing and managing these wounds.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Microbiology
Samuel Verbanic, John M. Deacon, Irene A. Chen
Summary: The factors that drive compositional shifts in wound microbiomes are still being uncovered. The virome, including bacteriophages, may play an important role in shaping bacterial community structure and function in chronic wounds. The wound virome is highly diverse, with many phages targeting known pathogens, and may influence bacterial community composition and functionality in ways that impact healing outcomes.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Andrea I. Medina Lopez, Daniel R. Fregoso, Anthony Gallegos, Daniel J. Yoon, Jaime J. Fuentes, Robert Crawford, Hawa Kaba, Hsin-ya Yang, R. Rivkah Isseroff
Summary: Non-healing wounds are a major medical challenge, and it has been found that beta-adrenergic antagonists can reduce the growth of common wound pathogens and limit biofilm formation. These findings provide new insights for the treatment of chronic skin wounds.
Article
Microbiology
Bryn Short, Ahmed Bakri, Abdullah Baz, Craig Williams, Jason Brown, Gordon Ramage
Summary: The management of chronic wounds is a challenging task and a burden on healthcare professionals and services. Chronic wounds are susceptible to microbial infection, which worsens their management and clinical outcomes. However, the role of fungi in chronic wounds is largely ignored, and their influence is not fully understood.
CURRENT CLINICAL MICROBIOLOGY REPORTS
(2023)
Article
Multidisciplinary Sciences
Brett Wagner Mackenzie, Melissa Zoing, Fiona Clow, David W. Waite, Fiona J. Radcliff, Michael W. Taylor, Kristi Biswas, Richard G. Douglas
Summary: The role of Staphylococcus aureus in chronic rhinosinusitis is still uncertain. Analysis showed no significant differences in S. aureus strain-level diversity between patients with and without CRS. Additionally, there may be an antagonistic interaction between S. aureus and D. pigrum.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, General & Internal
Hanna Luze, Ives Bernardelli de Mattos, Sebastian Philipp Nischwitz, Martin Funk, Alexandru Cristian Tuca, Lars-Peter Kamolz
Summary: In this study, the antimicrobial activity of antiseptic-loaded bacterial nanocellulose (BNC) against in vitro biofilms was investigated. The results showed that BNC loaded with Octenisept (R) could completely eradicate Staphylococcus aureus biofilm, while BNC loaded with Octenisept (R) or Prontosan (R) could also eliminate Candida albicans biofilm.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Vincent Falanga, Roslyn Rivkah Isseroff, Athena M. Soulika, Marco Romanelli, David Margolis, Suzanne Kapp, Mark Granick, Keith Harding
Summary: Chronic wounds are wounds that do not heal within an expected time frame due to prolonged dysregulated inflammation. Advances in understanding the cellular and molecular aspects of basic science and innovative treatment modalities have improved the management of chronic wounds. However, gaps still exist in scientific knowledge, highlighting the need for further research to enhance understanding of chronic wounds.
NATURE REVIEWS DISEASE PRIMERS
(2022)
Article
Microbiology
Sameer Gupta, Alexandra J. Poret, David Hashemi, Amarachi Eseonu, Sherry H. Yu, Jonathan D'Gama, Victor A. Neel, Tami D. Lieberman
Summary: Infections following cutaneous surgical procedures are rare, and the microbiome of normally healing surgical skin wounds has not been thoroughly studied. This research found that potentially pathogenic bacteria were suppressed in noninfected wounds, while members of the Corynebacterium genus were dominant. This suggests that commensal bacteria on the skin may play a role in reducing infection risk.
MICROBIOLOGY SPECTRUM
(2023)
Article
Gastroenterology & Hepatology
Ashish Chauhan, Ramesh Kumar, Sanchit Sharma, Mousumi Mahanta, Sudheer K. Vayuuru, Baibaswata Nayak, Sonu Kumar, Shalimar
Summary: Intestinal flora plays a critical role in immunity against HBV. FMT may be a potential immunomodulatory therapy in CHB patients. The study showed FMT could be effective in viral suppression and HBeAg clearance in HBeAg-positive CHB patients.
DIGESTIVE DISEASES AND SCIENCES
(2021)
Review
Immunology
Zoya Versey, Waleska Stephanie da Cruz Nizer, Emily Russell, Sandra Zigic, Katrina G. DeZeeuw, Jonah E. Marek, Joerg Overhage, Edana Cassol
Summary: Delayed wound healing can lead to chronic non-healing ulcers in individuals with immobility and co-morbid conditions, posing risks of infection and inflammation. The role of wound microbiome in driving dysregulated immune activation requires further investigation for developing effective therapeutics.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Plant Sciences
Brandon Kristy, Alyssa A. Carrell, Eric Johnston, Jonathan R. Cumming, Dawn M. Klingeman, Kimberly Gwinn, Kimberly C. Syring, Caroline Skalla, Scott Emrich, Melissa A. Cregger
Summary: This study investigates the effect of chronic drought stress on the belowground microbiomes of Populus trichocarpa trees. The results show that reduced irrigation influenced the composition of microbiomes, particularly bacteria, in the rhizosphere and bulk soil compartments. Drought-tolerant trees exhibited enriched growth-promoting microorganisms in the root endosphere and rhizosphere, which can be utilized to improve biofeedstock productivity in regions prone to periodic drought.
PHYTOBIOMES JOURNAL
(2022)
Review
Biotechnology & Applied Microbiology
Suhana Chattopadhyay, Leena Malayil, Emmanuel F. Mongodin, Amy R. Sapkota
Summary: Research indicates a wide range of bacterial and fungal microbiomes present in tobacco products, with some known human pathogens and others as potential opportunistic pathogens. Future studies should focus on the viability of these microorganisms and their potential transfer to the respiratory tract of users, potentially impacting their health.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Lindsay Kalan, Michael Loesche, Brendan P. Hodkinson, Kristopher Heilmann, Gordon Ruthel, Sue E. Gardner, Elizabeth A. Grice
Article
Infectious Diseases
Lindsay R. Kalan, Deanna M. Pepin, Imran Ul-Haq, Steve B. Miller, Michelle E. Hay, Roderick J. Precht
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2017)
Article
Dermatology
Michael Loesche, Sue E. Gardner, Lindsay Kalan, Joseph Horwinski, Qi Zheng, Brendan P. Hodkinson, Amanda S. Tyldsley, Carrie L. Franciscus, Stephen L. Hillis, Samir Mehta, David J. Margolis, Elizabeth A. Grice
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2017)
Article
Multidisciplinary Sciences
Lindsay Kalan, Mi Zhou, Michele Labbie, Benjamin Willing
Article
Cell Biology
Carly B. Deusenbery, Lindsay Kalan, Jacquelyn S. Meisel, Sue E. Gardner, Elizabeth A. Grice, Kara L. Spiller
WOUND REPAIR AND REGENERATION
(2019)
Article
Ecology
J. Z. Alex Cheong, Chad J. Johnson, Hanxiao Wan, Aiping Liu, John F. Kernien, Angela L. F. Gibson, Jeniel E. Nett, Lindsay R. Kalan
Summary: The study explored the overall biofilm community structure in a three-membered community derived from a chronic wound, showing that priority effects and inter-bacterial competition significantly shape the community structure. The attachment of Citrobacter freundii to Candida albicans is mediated by mannose-binding lectins, and co-cultures of these two species trigger neutrophil death and inflammation. These results enhance understanding of fungal-bacterial interactions and their impact on wound healing.
Article
Cell Biology
J. Z. Alex Cheong, Aiping Liu, Clayton J. Rust, Collin L. Tran, Sameeha E. Hassan, Lindsay R. Kalan, Angela L. F. Gibson
Summary: This study evaluated the cytotoxicity and antimicrobial activity of chlorhexidine gluconate (CHG), a wound cleansing agent, in human skin using clinically relevant models. The results showed that CHG significantly affected the metabolic activity of skin explants and had persistent cytotoxicity, impairing wound healing in vivo. CHG also had limited antimicrobial activity, with viable bacterial cells residing deep within wound tissue even after CHG application.
WOUND REPAIR AND REGENERATION
(2022)
Article
Microbiology
Mary Hannah Swaney, Shelby Sandstrom, Lindsay R. Kalan
Summary: The skin microbiome plays a crucial role in maintaining skin health, and cobamide sharing may mediate dynamics within skin microbial communities.
Review
Biochemistry & Molecular Biology
Elizabeth C. Townsend, Lindsay R. Kalan
Summary: Research has focused on identifying the balanced skin microbiome and developing therapeutics, but this ideal balance may not exist. The skin microbiome changes with age due to various factors. Local skin physiology, genetics, microbe-host interactions, and microbe-microbe interactions determine the precise composition of the skin microbiome for individuals. This review examines the factors that shape the skin microbiome throughout life and discusses the impact of disruptions to this balance on dermatological diseases and overall health.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Article
Microbiology
Rauf Salamzade, Mary Hannah Swaney, Lindsay R. Kalan
Summary: In this study, comparative genomics was used to investigate the genetic traits that differentiate a phylogenetically related group of Corynebacterium, the Corynebacterium tuberculostearicum species complex. The study found that different species within the complex are associated with specific body sites and that one species increases in abundance during atopic dermatitis flares.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Mary Hannah Swaney, Amanda Nelsen, Shelby Sandstrom, Lindsay R. Kalan
Summary: The microorganisms living on human skin have adapted to the challenging conditions that usually hinder microbial growth. They have developed a preference for high concentrations of sweat, while the preference for sebum varies among different bacterial species. These findings provide insights into the composition of skin microbiomes and underscore the importance of studying microorganisms in their natural environment.
MICROBIOLOGY SPECTRUM
(2023)
Article
Genetics & Heredity
Rauf Salamzade, J. Z. Alex Cheong, Shelby Sandstrom, Mary Hannah Swaney, Reed M. Stubbendieck, Nicole Lane Starr, Cameron R. Currie, Anne Marie Singh, Lindsay R. Kalan
Summary: A bioinformatics tool called lsaBGC was developed to analyze and compare biosynthetic gene clusters (BGCs) in bacterial taxa. By using lsaBGC, new insights into the evolution and diversity of BGCs in skin microbiomes were discovered. The tool identified the presence of a specific BGC in Staphylococcus aureus but its absence in Staphylococcus epidermidis. Additionally, novel single-nucleotide variants (SNVs) within BGCs of the Corynebacterium tuberculostearicum complex were identified.
MICROBIAL GENOMICS
(2023)
Review
Immunology
Mary Hannah Swaney, Lindsay R. Kalan
Summary: The human skin functions as a barrier against the external environment and provides protection for the skin microbiome, which includes resident microbiota. The cooperation between the microbiome, host skin cells, and the immune system is crucial for maintaining skin health. Disruption of this balance, such as pathogen invasion, can impair skin function.
INFECTION AND IMMUNITY
(2021)
Article
Biotechnology & Applied Microbiology
Joe A. Lemire, Lindsay Kalan, Natalie Gugala, Alexandru Bradu, Raymond J. Turner