Article
Chemistry, Physical
N. K. Murugasenapathi, K. A. Esther Jebakumari, S. Jamal Mohamed, K. Giribabu, Tamilarasan Palanisamy
Summary: In this study, the redox reaction of Nile Blue A on a glassy carbon surface was investigated using pinhole-free silica-coated silver nanoparticles for Raman signal enhancement. The interference from silver in the reaction mechanism was observed and the pinhole-free silica layer prevented hot electron transfer, providing interference-free enhancement to the Raman signals.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Sungje Bock, Yun-Sik Choi, Minhee Kim, Yewon Yun, Xuan-Hung Pham, Jaehi Kim, Bomi Seong, Wooyeon Kim, Ahla Jo, Kyeong-Min Ham, Sung Gun Lee, Sang Hun Lee, Homan Kang, Hak Soo Choi, Dae Hong Jeong, Hyejin Chang, Dong-Eun Kim, Bong-Hyun Jun
Summary: This paper reports a method to synthesize near-infrared (NIR) SERS nanoprobes SiO2@Au@Au NPs and achieve control over the nanogaps by adjusting the size of gold nanoparticles, resulting in effective SERS hotspots. SiO2@Au@Au NPs showed detectable in vivo SERS signals in animal tissue specimens and exhibited distinct SERS signals for different Raman label compounds injected into nude mice.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Optics
Chloe Duffield, Nana Lyu, Yuling Wang
Summary: This study identified the ideal conditions for bulk synthesis of silver-gold core-shell nanoparticles with a 2:1 ratio of silver nitrate to hydroquinone, resulting in stable, abundant, and highly reproducible Raman signals. After stability testing, the SERS nanotags showed stability with minimal signal change, indicating great potential for cancer and disease diagnosis.
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Fahad M. M. Aldosari
Summary: This study investigated the size dependency of gold nanoparticles (AuNPs) on their surface-enhanced Raman scattering (SERS) activity by using labeled AuNPs. The results showed that the SERS signal increases with the increasing size of AuNPs, and the preferred candidates for Raman labelling of spherical gold within the range of 5 nm to 100 nm are BPT and 4-AATP.
Article
Biochemical Research Methods
Stefano Fornasaro, Alessandro Esposito, Fiorella Florian, Alberto Pallavicini, Luigina De Leo, Tarcisio Not, Cristina Lagatolla, Marica Mezzarobba, Alessia Di Silvestre, Valter Sergo, Alois Bonifacio
Summary: Significant differences were found in the faecal samples of celiac patients and those on a gluten-free diet using SERS spectroscopy and 16S rRNA sequencing analysis. Although there were no significant differences in bacterial composition, the relative intensity of xanthine and hypoxanthine in the SERS spectra of patients on a gluten-free diet was significantly increased.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Hajir Hilal, Qiang Zhao, Jeongwon Kim, Sungwoo Lee, MohammadNavid Haddadnezhad, Sungjae Yoo, Soohyun Lee, Woongkyu Park, Woocheol Park, Jaewon Lee, Joong Wook Lee, Insub Jung, Sungho Park
Summary: This study demonstrates a new three-dimensional nanostructure with polarization-independent SERS activity, prepared through a precise synthesis method. This unique structure shows efficient interaction with light and analytes, providing new possibilities for optical-based sensing.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Caterina Dallari, Riccardo Innocenti, Elena Lenci, Andrea Trabocchi, Francesco Saverio Pavone, Caterina Credi
Summary: This study presents the synthesis of novel bioorthogonal Raman reporter-labeled nanoparticles using a versatile and cost-effective method, and demonstrates their potential for various applications in biological analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Xiao Liang, Pu Zhang, Minghai Ma, Tao Yang, Xiangwei Zhao, Rui Zhang, Minxuan Jing, Rundong Song, Lei Wang, Jinhai Fan
Summary: A ratiometric SERS assay was developed for non-invasive detection of bladder tumor, showing excellent stability, high sensitivity, high specificity, and potential for clinical diagnosis with superior results compared to current methods.
Article
Chemistry, Analytical
Nolan File, Joseph Carmicheal, Alexey V. Krasnoslobodtsev, Nicole C. Japp, Joshua J. Souchek, Sudesna Chakravarty, Michael A. Hollingsworth, Aaron A. Sasson, Gopalakrishnan Natarajan, Prakash G. Kshirsagar, Maneesh Jain, Chihiro Hayashi, Wade M. Junker, Sukhwinder Kaur, Surinder K. Batra
Summary: SERS immunoassay biosensors have great potential for clinical diagnostics due to their high sensitivity and the growing interest in multi-marker panels. However, difficulties in designing compatible extrinsic Raman labels have hindered their development. Previous studies have focused on selecting Raman reporter molecules (RRMs) based on their spectroscopic characteristics for multiplexing, as well-differentiated spectra are essential for simultaneous detection. However, these candidates often induce aggregation of SERS nanotags made of gold nanoparticles. Therefore, a better understanding of factors affecting the aggregation of RRM-coated gold nanoparticles is needed.
Article
Spectroscopy
Yan Dong, Wei Dong, Xin Liang, Yuan-Rui Wang, Feng Xu, Li Li, Lu Han, Li-Rui Jiang
Summary: A TB-activated fluorescence-surface-enhanced Raman spectroscopy (SERS) dual-mode optical nanoprobe (MRAu) was developed for the detection of thrombin. The nanoprobes combined SERS and fluorescence methods, allowing for a wider detection range of thrombin and a lower detection limit. The effectiveness and practicality of the nanoprobe were verified in human serum and it was also successfully used to evaluate the inhibitory effect of active components against thrombin in Panaa notoginseng.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Engineering, Environmental
Ting Wang, Shuangpeng Wang, Zehua Cheng, Jinchao Wei, Lele Yang, Zhangfeng Zhong, Hao Hu, Yitao Wang, Bingpu Zhou, Peng Li
Summary: Pesticide residues pose a significant threat to human health and the environment, driving the need for rapid and effective detection methods. Core-shell nanostructures as novel SERS substrates have been widely utilized in rapid pesticide detection research. Understanding and monitoring pesticide residues can be improved through the classification and evaluation of different core-shell nanostructures and early-warning analysis.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Nanoscience & Nanotechnology
Ammar Nayfeh, Ayman Rezk, Noha Elhalawany, Majid Al Ruqeishi, Adem Kocyigit, Ersin Bahceci, Munir Hasan Nayfeh
Summary: Metal nanostructures can create near-field super hotspots under light irradiation, which can enhance nonlinear optical processes. By simulating concentric layered silicon-metal core-shell nanostructures, it is found that super hotspots can emerge at the silicon-vacuum interface and be tuned by adjusting the silicon thickness. Additionally, the plasmonic resonance red shifts with the thickness of the silicon shell, providing potential applications for Raman scattering and photovoltaics.
Article
Nanoscience & Nanotechnology
Lan Thi Dang, Huy Le Nguyen, Hai Van Pham, Mai Thi Tuyet Nguyen
Summary: In this report, Au@Ag core-shell nanorod structures were developed for contaminants sensing by SERS. The silver shell thickness and plasmon wavelength were tuned by changing coating time and silver precursor amount. These structures showed ultra-sensitive detection ability for Nile blue A dye and Fenobucarb pesticide sensing. The Au@Ag core-shell sample with minimal silver shell thickness exhibited the highest Raman enhancement factor due to optimal enhancement of the electromagnetic field of bimetallic structures. This report demonstrates the potential applicability of combining unique features of two plasmonic metals in SERS-based analysis.
Article
Chemistry, Analytical
Zhi-Yong Guo, Chen Zhang, Lin-min Chen, Mei-huang Zeng, Qiu-hong Yao, Ting-Xiu Ye, He-zhou Luo, Xiao-mei Chen, Xi Chen
Summary: Shell-isolated colloid plasmonic nanomaterials-based nanoreactor is a versatile platform widely used in catalyst or SERS sensors. A competitive conjugative-mediated nanoreactor is introduced to determine glucose with SERS, which constructs glucose-conjugating nanoreactor using coordinated deposition colloidal gold nanoparticles with sodium nitroprusside framework (Au@SNF) and covalently bonded 4-mercaptopyridine (4-Mpy). The nanoreactor combines well-defined structure, conjugative-mediated mechanism, and internal standards method for improved signal indication and correction, enabling quantitative SERS detection of glucose in complex samples.
Article
Chemistry, Analytical
Ding Wang, Yiru Zhao, Shen Zhang, Liping Bao, Huijun Li, Jingcheng Xu, Bin He, Xumin Hou
Summary: This study developed a rapid and accurate method for the detection of acute myocardial infarction using surface-enhanced Raman scattering (SERS) technique. By preparing 4-Mercaptobenzoic acid embedded Au@Ag core-shell nanospheres, the detection of cardiac troponin I (cTn I) was successfully achieved. The prepared SERS nanotags could complete the analysis procedure within 15 minutes, indicating a faster detection speed and higher accuracy.
Article
Spectroscopy
Rafael J. G. Rubira, Sabrina A. Camacho, Carlos J. L. Constantino, Santiago Sanchez-Cortes
Summary: In this study, silver nanoparticles were utilized as SERS substrates to detect commercial samples of pesticides. The strong interaction between amino groups and oxygenated groups was confirmed to enhance the sensitivity of SERS detection.
JOURNAL OF RAMAN SPECTROSCOPY
(2022)
Article
Chemistry, Applied
Eloi Feitosa, Renata D. Adati, Carlos J. L. Constantino, Pedro H. B. Aoki
Summary: The study found that there is an exchange interaction between DODAB monolayers and vesicles at different concentrations, especially more significant at higher vesicle concentrations.
JOURNAL OF SURFACTANTS AND DETERGENTS
(2022)
Article
Biochemistry & Molecular Biology
Lucas S. A. Pereira, Sabrina A. Camacho, Alexandre M. Almeida, Renato S. Goncalves, Wilker Caetano, Christine DeWolf, Pedro H. B. Aoki
Summary: In this study, the interaction between the photosensitizer hypericin and different cell membranes was investigated. The results showed that hypericin had a strong binding and penetration ability in DPPC and DOPC monolayers, while electrostatic repulsions played a major role in the interactions with DOPG and DOPS. The irradiation of DOPC monolayers containing hypericin resulted in material loss due to hydrocarbon chain cleavage, while the effects on irradiated DOPS and DOPG monolayers were mild and attributed to hypericin self-aggregation.
CHEMISTRY AND PHYSICS OF LIPIDS
(2022)
Article
Biophysics
Sabrina A. Camacho, Mirella B. Kobal, Lucas G. Moreira, Maria J. Bistaffa, Thamires C. Roque, Wallance M. Pazin, Karina A. Toledo, Osvaldo N. Oliveira Jr, Pedro H. B. Aoki
Summary: The use of photoexcited gold shell-isolated nanoparticles (AuSHINs) has been found to effectively reduce the viability of tumor cells while causing minimal damage to healthy cells in vitro. These effects can be explained by the different interactions of AuSHINs with tumor and healthy cell lipid extracts at the molecular level. AuSHINs penetrate deeper into tumor cell membranes, affecting the lipid chain cleavage and causing more significant photoinduced damage compared to healthy cells.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Physical
Nathan C. Lindquist, Ariadne Tuckmantel Bido, Alexandre G. Brolo
Summary: This paper investigates the mechanism of fluctuations in Surface Enhanced Raman Spectroscopy (SERS) signals and finds that the underlying source of these fluctuations is a complex interplay of different effects. High-speed SIF activity is present in both dry and wet nanoparticle environments, and a simple model is proposed to explain the contributions to the fluctuations.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Chemistry, Analytical
Pearl Thompson, Ana Livia de Carvalho Bovolato, Gisela Ibanez-Redin, Alexandre G. Brolo
Summary: A rapid lateral flow screening tool using saliva samples and cell phone quantification has been developed, providing sensitive detection of IgG antibodies against SARS-CoV-2 within 10 minutes.
Article
Chemistry, Analytical
Ariadne Tuckmantel Bido, Alexandre G. Brolo, Arash Azarakhshi
Summary: Surface-enhanced Raman scattering (SERS) is a sensitive spectroscopic technique, but its reproducibility has been an issue in analytical chemistry applications. This study demonstrates that reliable quantification of SERS immunoassay at low concentrations is possible with careful experimental design and appropriate data analysis statistics.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Minh-Hai Tran, Ian Booth, Bruno G. daFonseca, Peter Berrang, Jeremy E. Wulff, Alexandre G. Brolo
Summary: An economical and facile method has been developed to synthesize a precursor for carbon films and materials. This precursor can be easily coated onto substrates and converted into a graphitic-like structure after mild thermal treatment. This method allows for the coating of glass surfaces of different shapes and forms.
Article
Chemistry, Multidisciplinary
Makayla M. Schmidt, Emily A. Farley, Marit A. Engevik, Trey N. Adelsman, Ariadne Tuckmantel Bido, Nathan D. Lemke, Alexandre G. Brolo, Nathan C. Lindquist
Summary: The concept of plasmonic hotspots is central to nanophotonics, specifically in surface-enhanced Raman scattering (SERS). SERS signals from single molecules often exhibit fluctuations, challenging the concept of static hotspots. Recent experiments have shown SERS intensity fluctuations (SIFs) to occur over a wide range of timescales due to various physical mechanisms causing SERS and the dynamic nature of light-matter interaction. This study presents a high-speed acquisition system that captures SERS spectra with microsecond time resolution, revealing that high-speed SIF events occur with relatively equal probability over a broad spectral range.
Article
Instruments & Instrumentation
Kirsty Milligan, Kendra Scarrott, Jeffrey L. Andrews, Alexandre G. Brolo, Julian J. Lum, Andrew Jirasek
Summary: Raman spectroscopy is a useful tool for obtaining biochemical information, but interpreting the data can be challenging. Our group has demonstrated a non-negative matrix factorization framework as an alternative to dimensionality reduction techniques for Raman spectroscopy data related to radiation response monitoring. We evaluated the accuracy of the model in reconstructing mixture solutions of known concentrations and considered factors such as spectral bases, noise thresholds, and different biochemical groups. Overall, the model performed well and solid bases spectra were comparable to solution bases spectra. The inclusion of an unconstrained component did not significantly affect the deconstruction, as long as all biochemicals were included in the model.
APPLIED SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Moyra F. Vieira, Ana Livia de Carvalho Bovolato, Bruno G. da Fonseca, Celly M. S. Izumi, Alexandre G. Brolo
Summary: A biosensor was developed for directly detecting human immunoglobulin G (IgG) and adenosine triphosphate (ATP) using gold nanoparticles/polystyrene-b-poly(2-vinylpyridine) (AuNP/PS-b-P2VP) nanocomposites. The substrates were functionalized with carboxylic acid groups to bind anti-IgG and anti-ATP and detect IgG and ATP. SEM images showed gold nanoparticles adsorbed onto a porous thin film. UV-VIS and SERS were used to characterize substrate functionalization and antibody-target interaction. PCA discriminated between samples before and after affinity tests. The biosensor was sensitive to IgG concentrations with a limit of detection of 1 μg/mL.
Article
Spectroscopy
Bruno G. daFonseca, Sapanbir S. Thind, Ian Booth, Alexandre G. Brolo
Summary: Principal component analysis (PCA) and linear discriminant analysis (LDA) were used to classify different types of carbon material based on their Raman spectra, providing an avenue for automated classification of carbon materials using Raman spectroscopy and machine learning.
JOURNAL OF RAMAN SPECTROSCOPY
(2023)
Article
Chemistry, Multidisciplinary
Minh-Hai Tran, Ian Booth, Arash Azarakhshi, Peter Berrang, Jeremy Wulff, Alexandre G. Brolo
Summary: This paper introduces a new method for large-scale synthesis of graphene that minimizes the creation of structural defects. The method involves hydrogen functionalization of graphite followed by thermal dehydrogenation, and it is demonstrated to be effective for the preparation of high-quality graphene films.
Article
Chemistry, Analytical
Ariadne Tuckmantel Bido, Katherine J. I. Ember, Dominique Trudel, Madeleine Durand, Frederic Leblond, Alexandre G. Brolo
Summary: The SARS-CoV-2 pandemic has posed a challenge in containing the spread of the virus, especially in low and middle-income countries due to limited access to screening tests. In this study, a low-cost biosensor device based on localized surface plasmon resonance (LSPR) was developed for the screening of SARS-CoV-2. The sensor showed high sensitivity and specificity in detecting the virus in saliva samples, providing a potential solution for screening challenges in underserved communities.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Makayla M. Schmidt, Emily A. Farley, Marit A. Engevik, Trey N. Adelsman, Ariadne Tuckmantel Bido, Nathan D. Lemke, Alexandre G. Brolo, Nathan C. Lindquist
Summary: The concept of plasmonic hotspots plays a crucial role in the field of nanophotonics, particularly in surface-enhanced Raman scattering (SERS). Recent experiments have shown that these hotspots exhibit significant fluctuations at different timescales, which are likely caused by the complex interplay of multiple effects. This study presents a high-speed acquisition system that captures the dynamic processes of SERS fluctuations and finds that these fluctuations occur over a wide spectral range.
