Article
Chemistry, Multidisciplinary
Xinchun Ran, Yaoyukun Jiang, Qianzhen Shao, Zhongyue J. Yang
Summary: EnzyKR is a new approach based on a deep learning framework for predicting enantiomeric outcomes in hydrolase-catalyzed kinetic resolution reactions. It automates the selection of enzyme scaffolds for separating a racemic substrate mixture and achieves good prediction performance using a structure-based encoding strategy.
Article
Biophysics
Mostafa Mostafavi, Kazem Mahmoodzadeh, Zohreh Habibi, Maryam Yousefi, Jesper Brask, Mehdi Mohammadi
Summary: Neutrase hNFs are prepared by mixing metal ion aqueous solutions with Neutrase in a phosphate buffer, resulting in precipitation. These hNFs exhibit excellent catalytic activity and stability, and can be used as reusable catalysts for coupling reactions under mild conditions.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Green & Sustainable Science & Technology
Le Zhong, Xiaobo Jiao, Hongtong Hu, Xuejian Shen, Juan Zhao, Yuxiao Feng, Conghai Li, Yingjie Du, Jiandong Cui, Shiru Jia
Summary: In this study, surfactant activated lipase from Aspergillus oryzae was used to prepare magnetic hybrid nanoflowers with Fe3O4 magnetic nanoparticles embedded, showing high catalytic efficiency, tolerance, and reusability. The magnetic hybrid nanoflowers exhibited excellent performance in producing biodiesel from soybean oil, with a high yield even after multiple cycles of reuse.
Article
Chemistry, Physical
Hossein Jafari-Nodoushan, Somayeh Mojtabavi, Mohammad Ali Faramarzi, Nasrin Samadi
Summary: Organic-inorganic hybrid nanoflowers (HNFs) are hierarchical flower-shaped microstructures composed of organic and inorganic constituents. They have a high surface-to-volume ratio and are mainly used for efficient biocatalysis and highly sensitive biosensing. They also have other important applications. However, there are still unknown aspects and future directions of research in this field.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Article
Microbiology
Saori Kataoka, Sayuri Kawamoto, Kazunobu Tsumura, Kazuhiko Ishikawa
Summary: The interesterification activity of lipases was estimated using a water-in-oil emulsion system. By comparing the sequence and crystal structure data of the lipases, it was determined that two amino acids near the active center are one of the factors controlling the hydrolytic and interesterification activities of the enzyme.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Natividad Ortega, Laura Saez, David Palacios, Maria D. Busto
Summary: This study focuses on the thermal behavior and stability of enzymes, specifically Lipase PS and Palatase, at different temperatures. Kinetic modeling revealed that the first-order model and Weibull distribution were the most suitable equations to describe the residual activity of the enzymes, with Lipase PS exhibiting higher thermal stability compared to Palatase. The activation energy values and thermodynamic parameters provided insights into the nature and behavior of the enzymes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Shuhui Wu, Panliang Zhang, Bizhu Sun, Ying Wu, Meiai Huang, Zhuolin Luo, Yingnan Ma, Kewen Tang
Summary: Lipase PS from Pseudomonas cepacia was immobilized on a new support prepared with metal-organic framework (MOF) and polyethyleneimine (PEI), resulting in higher specific activity and enhanced enzyme-substrate affinity compared to free lipase PS. Under optimized conditions, PS@MOF-5-PEI achieved high enantioselectivity and catalytic efficiency for kinetic resolution of TFMP enantiomers.
APPLIED CATALYSIS A-GENERAL
(2023)
Article
Engineering, Environmental
Rong Wang, Ruixi Liu, Shijia Luo, Rui Chen, Fei Li, Jing Yang, Qiang Zhang, Jianlong Wang
Summary: Organic-inorganic hybrid nanoflowers have shown great potential in catalysis, but their photocatalytic sterilization effects have not been fully studied. This study demonstrates that adjusting the band gap and increasing the content of bovine serum albumin (BSA) can enhance the photocatalytic sterilization performance of nanoflowers. Moreover, the material also has a significant impact on bacterial wound healing.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Chemical
Fatemeh Ahmadpoor, Mahmoud Nasrollahzadeh, Mahsa Mohammad
Summary: This study successfully designed an efficient and green hierarchical copper phosphate-lignosulfonate nanoflowers using an eco-friendly self-assembly method for catalytic reduction of organic dyes in water. Lignosulfonate played a critical role in controlling the size and morphology of Cu-LS nanoflowers, which showed high stability and reusability in catalytic tests.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Review
Chemistry, Applied
Li-Cheng Yang, Heping Deng, Hans Renata
Summary: This article summarizes the chemoenzymatic and biocatalytic methodologies for DKR developed in the past decade, highlighting their applications in the preparation of active pharmaceutical ingredients and emerging paradigms.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2022)
Article
Biochemistry & Molecular Biology
Mostafa Mostafavi, Mahtab Beihaghi Poor, Zohreh Habibi, Mehdi Mohammadi, Maryam Yousefi
Summary: In this study, 3D flower-like structured organic-inorganic hybrid nanoflowers (hNFs) with various lipases and graphene quantum dots (GQDs) were prepared and characterized. The addition of GQDs significantly increased the activity of the lipase hNFs. The lipase@GQDs hNFs showed higher activity compared to free lipase and other hNFs without GQDs, and also exhibited improved pH and thermal stability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Chemistry, Applied
Dan-Yang Li, Yu-Jiao Lou, Jian Xu, Xiao-Yang Chen, Xian-Fu Lin, Qi Wu
Summary: Using an electronic effect-guided rational design strategy, significant improvement of enantioselectivity towards bulky pyridyl(phenyl)methanols is achieved in CALB mutants, leading to successful kinetic resolution of various phenyl pyridyl methyl acetates with good yields and ee values.
ADVANCED SYNTHESIS & CATALYSIS
(2021)
Article
Chemistry, Physical
Jiaojiao Gao, Hui Liu, Kexin Wu, Jifeng Yan, Huayu Li, Ruixuan Yang, Cheng Tong, Lingyan Pang, Junqi Li
Summary: In this study, dual-functional nanoflowers Cu-NFs were prepared through a simple method and utilized to construct a sensitive colorimetric biosensor for the detection of carcinoembryonic antigen. The colorimetric sensor showed a wide linear range and low detection limit, with successful application in human serum samples, suggesting promising applications in medical diagnosis and portable detection.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jacek Duleba, Tomasz Siodmiak, Michal Piotr Marszall
Summary: This study investigated the immobilization of Amano lipase PS from Burkholderia cepacia onto a polyacrylic support, which resulted in improved activity in nonaqueous media. The enantioselectivity of the immobilized lipase was evaluated in the kinetic resolution of (R,S)-1-phenylethanol, and the effects of different non-aqueous solvents were assessed. The study also proposed a novel method to study the lipolytic activity of the immobilized lipase using multicomponent unsaturated fatty acids in an aqueous medium.
PROCESS BIOCHEMISTRY
(2022)
Review
Materials Science, Biomaterials
Xiao Liang, Yong Liu, Kai Wen, Wei Jiang, Quanshun Li
Summary: Enzyme immobilization in inorganic hybrid nanoflowers is a biomimetic mineralization method with mild reaction conditions, which helps to obtain ideal biocatalysts. Immobilized enzymes in inorganic hybrid nanoflowers have been successfully used in biocatalysis, producing medical intermediates and biodiesel, as well as in developing biosensors for detecting toxic substances and disease biomarkers.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Biochemistry & Molecular Biology
Shweta Tiwari, Seema Kirar, Uttam Chand Banerjee, Kishore Babu Neerupudi, Sushma Singh, Aabid Abdullah Wani, Prasad Bharatam, Inder Pal Singh
BIOORGANIC CHEMISTRY
(2020)
Article
Biotechnology & Applied Microbiology
Gopal Patel, Neeraj Singh Thakur, Varun Kushwah, Mahesh D. Patil, Shivraj Hariram Nile, Sanyog Jain, Uttam Chand Banerjee, Guoyin Kai
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Surbhi Soni, Bharat P. Dwivedee, Uttam C. Banerjee
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Acoustics
Gopal Patel, Mahesh D. Patil, Sujit Tangadpalliwar, Shivraj Hariram Nile, Prabha Garg, Guoyin Kai, Uttam Chand Banerjee
Summary: This study focused on modeling and optimizing the production of mycophenolic acid (MPA) from Penicillium brevicompactum using ultrasonic stimulation. By optimizing the ultrasonic treatment conditions, the MPA production was significantly improved.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Amit Kumar Mittal, Uttam Chand Banerjee
Summary: This study synthesized inorganic nanoparticles of various sizes and shapes using plant extracts and phytochemicals, and evaluated their safety, toxicity and efficacy through a series of biological and histopathological assays. Results showed that the nanoparticles exhibited high therapeutic effectiveness with minimal toxic effects on organisms, suggesting their potential for emergency management of life-threatening conditions.
MATERIALS TODAY COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Seema Kirar, Dasharath Chaudhari, Neeraj S. Thakur, Sanyog Jain, Jayeeta Bhaumik, Joydev K. Laha, Uttam C. Banerjee
Summary: A novel A4 type porphyrin photosensitizer was successfully synthesized and encapsulated into gelatin nanoparticles for the development of hydrophilic phototherapeutic nanoagents. The synthesized nanoprobes exhibited good fluorescence performance, indicating potential application in bioimaging. The results of the study demonstrated that these nanoprobes can serve as effective phototherapeutic agents against cancer cells and microbes.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Neeraj S. Thakur, Narattam Mandal, Gopal Patel, Seema Kirar, Y. Nikhileshwar Reddy, Varun Kushwah, Sanyog Jain, Yogeshvar N. Kalia, Jayeeta Bhaumik, Uttam C. Banerjee
Summary: The co-encapsulation of zinc phthalocyanine and quercetin in lipid polymer hybrid nanoparticles significantly enhances the photodynamic anticancer activity. This strategy demonstrates good drug delivery and antitumor effects in in vivo experiments, offering promise as an important approach for future cancer therapy.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Dharam Pal, Gopal Patel, Prakashkumar Dobariya, Shivraj Hariram Nile, Abhay H. Pande, Uttam Chand Banerjee
Summary: In this study, a codon-optimized gene encoding for rhIFN-beta((C17S)) protein was designed and expressed in E. coli SE1. The optimized medium with specific components was found to provide better growth of recombinant cells and increased expression of the protein compared to traditional medium, showing potential for large-scale production of rhIFN-beta.
Review
Chemistry, Multidisciplinary
Seema Kirar, Neeraj Singh Thakur, Yeddula Nikhileshwar Reddy, Uttam Chand Banerjee, Jayeeta Bhaumik
Summary: Nanophotosensitizers have the potential to selectively destroy microbial and cancerous cells through the generation of reactive oxygen species via light irradiation. Tetrapyrrolic macromolecules such as porphyrins and phthalocyanines possess unique photophysical properties but also have limitations like low water solubility and lack of specificity. Researchers are working on incorporating photosensitizers into different types of nanoparticles to improve photodynamic therapy quality.
JOURNAL OF PORPHYRINS AND PHTHALOCYANINES
(2021)
Article
Chemistry, Medicinal
Shailendra Sisodiya, Subarno Paul, Hiteshkumar Chaudhary, Preeti Grewal, Gulshan Kumar, Divine P. Daniel, Biswajit Das, Deepika Nayak, Sankar K. Guchhait, Chanakya N. Kundu, Uttam C. Banerjee
Summary: Two series of compounds were synthesized and screened against cancer cells, with some showing significant apoptotic anti-proliferation effects on cancer cells; they also exhibited relatively less cytotoxicity to normal cells and showed more efficiency in hTopoII alpha inhibition compared to the anticancer drug etoposide.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Linga Banoth, Kezia Devarapalli, Indrani Paul, Karuna Narayan Thete, Sandip Pawar, Uttam Chand Banerjee
Summary: This study isolated a microorganism producing enantioselective lipase for kinetic resolution of racemic alcohols, successfully improving lipase activity and growth conditions. Lipase production and resolution of alcohol compounds were achieved in laboratory scale and bioreactor.
JOURNAL OF THE INDIAN CHEMICAL SOCIETY
(2021)
Review
Chemistry, Physical
Sahil Verma, Rahul Narayanlal Choudhary, Akash Prakash Kanadje, Uttam Chand Banerjee
Summary: Hydrolases, particularly lipases, play a significant role in the pharmaceutical industry by catalyzing the hydrolysis of ester bonds with high specificity and robustness, making them suitable for various organic synthesis procedures. Genetic engineering has further enhanced the catalytic capabilities of lipases, allowing for the development of customized enzymes for greener biocatalytic drug synthesis. Lipases may also play a key role in developing cascade reactions in organic synthesis, making them a promising candidate for future biocatalytic research in the pharmaceutical industry.
Article
Biotechnology & Applied Microbiology
Shraddha Lambhiya, Gopal Patel, Uttam Chand Banerjee
Summary: This study isolated and partially purified transaminase from wild Bacillus licheniformis and immobilized it on copper nanoflowers to enhance its kinetic properties and stability. The immobilized transaminase showed high reusability and potential application in resolving racemic alpha-methyl benzylamine.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Chemistry, Multidisciplinary
Shweta Tiwari, Seema Kirar, Uttam Chand Banerjee, Neerupudi Kishore Babu, Sushma Singh, Inder Pal Singh
JOURNAL OF THE INDIAN CHEMICAL SOCIETY
(2020)
Article
Biotechnology & Applied Microbiology
Gopal Patel, Taresh P. Khobragade, Sachin R. Avaghade, Mahesh D. Patil, Shivraj Hariram Nile, Guoyin Kai, Uttam Chand Banerjee
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2020)