Article
Polymer Science
Laura Riacci, Angela Sorriento, Leonardo Ricotti
Summary: This study investigated the properties of a novel jellyfish material JellaGel(TM) crosslinked with genipin for embedding human chondrocytes. The hydrogels crosslinked with 1 mM genipin showed higher stability and metabolic activity of chondrocytes. The results suggest a positive impact of genipin on cartilage regeneration hydrogels.
Article
Polymer Science
Sagun Chanmangkang, Sutee Wangtueai, Nantipa Pansawat, Pramvadee Tepwong, Atikorn Panya, Jirawan Maneerote
Summary: This study investigated the extraction of collagens from skipjack tuna tail tendons and evaluated their physiochemical properties and characteristics. The collagens were extracted using vinegar, acetic acid, and pepsin. The results showed that the collagens had good solubility, especially at low pH. The FTIR spectrum indicated the presence of various functional groups, and SEM analysis revealed the microstructure of the collagen surface.
Article
Biochemistry & Molecular Biology
Yanru Ren, Luo Liu, Xin Xiong, Rumen Krastev, Ralf Smeets, Denis Rimashevskiy, Reinhard Schnettler, Said Alkildani, Steffen Emmert, Ole Jung, Mike Barbeck
Summary: This study compared well plates coated with collagen type 0 derived from Rhizostoma pulmo with plates coated with bovine and porcine collagen for cytocompatibility analysis in vitro. The results showed that the well plates coated with jellyfish collagen had similar cytocompatibility to those coated with mammalian collagens, indicating its suitability for in vitro analysis of biomaterials or medical devices.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Igor S. Brum, Carlos N. Elias, Jorge J. de Carvalho, Jorge L. S. Pires, Mario J. S. Pereira, Ronaldo S. de Biasi
Summary: Dental implant treatment requires sufficient bone volume in the site for mechanical stability; when insufficient, surgical techniques like GBR are necessary. Resorbable collagen type I membranes were manufactured for GBR, showing no cytotoxicity and high biocompatibility in both in vitro and in vivo tests. This membrane could be a suitable choice for GBR procedures.
Article
Biotechnology & Applied Microbiology
Rory Gibney, Eleonora Ferraris
Summary: The study utilized aerosol jet printing to process collagen type I and II into dense constructs with suitable mechanical properties for replicating dense collagenous connective tissues. Collagen type II exhibited lower viscosity compared to type I, and both types experienced a drop in viscosity due to aerosol jet printing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Pedro U. Munoz-Gonzalez, Maria C. Lona-Ramos, Luis D. Gutierrez-Verdin, Guadalupe H. Luevano-Colmenero, Fernando Tenorio-Rocha, Rene Garcia-Contreras, Gerardo Gonzalez-Garcia, Argelia Rosillo-de la Torre, Jorge Delgado, Laura E. Castellano, Birzabith Mendoza-Novelo
Summary: The study evaluated the effects of oligourethane and colloidal silica particles on collagen material properties and skin wound healing in rats, concluding that collagen-based composite dressings have promising modulatory effects in skin wound healing.
BIOMEDICAL MATERIALS
(2022)
Article
Astronomy & Astrophysics
Woong-Bae G. Zee, Suk-Jin Yoon, Jun-Sung Moon, Sung-Ho An, Sanjaya Paudel, Kiyun Yun
Summary: Warped disk galaxies can be classified into S and U types. This study found that U types have bluer optical colors and a higher specific star formation rate (sSFR) compared to S types, with more strongly warped U types having an even higher sSFR. It was also observed that the tidal force by the nearest neighbor correlates with S-type warps regardless of the environment, but there is no such correlation for U types in groups/clusters, indicating a possible non-tidal environmental influence such as ram pressure stripping (RPS). U types are more common in groups/clusters and have similar properties to RPS-induced jellyfish galaxies in terms of stellar mass, gas fraction, sSFR enhancement, and phase-space distribution. The study suggests that RPS can be an alternative explanation for U types in groups/clusters, although the majority of U types in fields remain unexplained.
ASTROPHYSICAL JOURNAL
(2022)
Article
Polymer Science
Nur Izzah Md Fadilah, Nazeha Ahmat, Looi Qi Hao, Manira Maarof, Nor Fadilah Rajab, Ruszymah Binti Hj Idrus, Mh Busra Fauzi
Summary: This study aimed to validate the biological safety of ovine tendon collagen type-I (OTC-I) under ISO and GLP settings. OTC-I was successfully extracted using acetic acid, and tests showed no adverse effects or abnormalities. Therefore, OTC-I is a potential medical device candidate for wound care management.
Article
Chemistry, Medicinal
Malachy Maher, Veronica Glattauer, Carmine Onofrillo, Serena Duchi, Zhilian Yue, Timothy C. Hughes, John A. M. Ramshaw, Gordon G. Wallace
Summary: This study compares the potential and performance differences of collagen from marine and porcine sources in tissue engineering. Collagen derived from marine sources has lower risk of disease transfer and is more compatible with religious and ethical groups. Although it has inferior mechanical properties and is prone to degradation, marine-derived collagen is still considered a promising biomaterial.
Article
Biochemistry & Molecular Biology
Sarah M. Ali, Nashaita Y. Patrawalla, Nilabh S. Kajave, Alan B. Brown, Vipuil Kishore
Summary: Collagen methacrylation is a promising method for generating cell-laden hydrogels with improved mechanical properties. In this study, the effects of collagen species, collagen extraction methods, and photoinitiators on the physical properties of methacrylated collagen hydrogels were investigated. Human collagen showed the highest methacrylation degree, and lithuim phenyl-2,4,6-trimethylbenzoylphosphinate was more cytocompatible than Irgacure-2959. The rat methacrylated collagen hydrogels had higher compressive modulus and cell viability compared to bovine collagen hydrogels. Human methacrylated collagen hydrogels demonstrated superior print fidelity.
Article
Cell Biology
Nicola L. Stevenson, Dylan J. M. Bergen, Yinhui Lu, M. Esther Prada-Sanchez, Karl E. Kadler, Chrissy L. Hammond, David J. Stephens
Summary: Knockout of the golgin giantin leads to skeletal and craniofacial defects due to changes in glycosylation and extracellular matrix deposition. Our study reveals a conserved role for giantin in collagen biosynthesis and extracellular matrix assembly, as well as a giantin-dependent pathway for intracellular procollagen processing.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Orthopedics
Charlie M. M. Waugh, Rouhollah Mousavizadeh, Jenny Lee, Hazel R. C. Screen, Alexander Scott
Summary: Hypercholesterolemia is associated with tendon pathology and injury prevalence. Lipids can accumulate in the tendon's extracellular spaces, which may disrupt its hierarchical structure and the tenocytes physicochemical environment. We hypothesized that the tendon's ability to repair after injury would be attenuated with elevated cholesterol levels, leading to inferior mechanical properties.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Chemistry, Medicinal
Eva Martins, Gabriela S. Diogo, Ricardo Pires, Rui L. Reis, Tiago H. Silva
Summary: Marine collagen and silica-based materials have been investigated as potential biomaterials for tissue engineering. The study confirmed the biocompatibility of these materials and their ability to support cell adhesion and proliferation.
Review
Chemistry, Medicinal
Isabella D'Ambra, Louise Merquiol
Summary: The review explores the potential use of jellyfish as a sustainable source of high-value compounds. It suggests that jellyfish can be used to obtain various valuable products, but their exploitation needs to be optimized due to seasonal fluctuations in abundance.
Article
Chemistry, Applied
Yadong Zhao, Kunyu Lu, Xinyue Piao, Yan Song, Libin Wang, Rusen Zhou, Pingping Gao, Heng Yen Khong
Summary: This study evaluated the effect of adding collagen on the gel properties of surimi and observed the influence of collagen type for the first time. Compared to type II, collagen type I has higher water solubility and more charged amino acids, which allows it to better intertwine with surimi myofibrillar proteins, resulting in higher exposure of protein functional domains, more conformational changes of myosin, and stronger formation of chemical forces among proteins. These improvements accelerate the gelation rate, leading to a well-stabilized surimi gel. Surimi gels containing collagen I have more compact structures with uniformly distributed smaller pores compared to those containing collagen II, resulting in higher water holding capacity and better texture. The fortification performance of collagen I in surimi gel and the elucidated interaction mechanism between collagen and myofibrillar protein will guide the further use of collagen as an effective additive in the food industry.
Article
Biochemistry & Molecular Biology
Alexandra Paul, Belen Chanclon, Cecilia Brannmark, Pernilla Wittung-Stafshede, Charlotta S. Olofsson, Ingrid Wernstedt Asterholm, Sapun H. Parekh
Summary: Brown adipose tissue (BAT) is highly metabolically active and can catabolize nutrients to produce heat. Studies have shown that dietary fatty acids can regulate lipid deposition in BAT, similar to white adipose tissue (WAT). This study aimed to compare the effects of sustained dietary intervention on lipid metabolism and deposition in WAT and interscapular BAT.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Chemistry, Physical
Andrew P. Carpenter, Patricia Khuu, Tobias Weidner, Colin P. Johnson, Steven J. Roeters, Joe E. Baio
Summary: Dysferlin is a critical protein involved in repairing muscle injuries by recruiting vesicles to the injured site. Studies show that its N-terminal C2A domain can change its binding orientation based on the lipid composition of the membrane.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Review
Materials Science, Biomaterials
Shivani Chaudhary, Doyel Ghosal, Pravesh Tripathi, Sachin Kumar
Summary: The properties of biomaterials significantly influence a cell's ability to adhere to its surface and regulate cell functioning. No generalized rule has been established to predict cellular processes on biomaterial surfaces. Recent studies have identified cellular metabolism as another critical aspect that regulates cell behavior. This review highlights how different physiochemical properties of scaffolds influence cell metabolism and emphasizes the importance of cell metabolism as a guiding principle for tissue engineering.
BIOMATERIALS SCIENCE
(2023)
Article
Spectroscopy
Mette H. Rasmussen, Cherno Jaye, Daniel Fischer, Tobias Weidner
Summary: Calcium minerals are widely distributed in geology and life chemistry, and understanding their phase and chemical state is crucial for various processes. Photoemission spectroscopies such as NEXAFS and scanning transmission X-ray microscopy have been instrumental in identifying and characterizing calcium minerals. This study presents a comprehensive list of reference spectra for different calcium minerals obtained through imaging with a NEXAFS microscope.
JOURNAL OF ELECTRON SPECTROSCOPY AND RELATED PHENOMENA
(2023)
Article
Chemistry, Multidisciplinary
Jan Thogersen, Adam S. Chatterley, Tobias Weidner, Frank Jensen
Summary: The susceptibility of aqueous dipeptides to photodissociation by deep ultraviolet irradiation was investigated using femtosecond spectroscopy and density functional theory calculations. The primary photo dynamics showed that most of the excited dipeptides withstood the deep ultraviolet excitation, while a small portion dissociated by decarboxylation. When dissociation occurred, the peptide bond remained intact and subsequent reactions were possible. The low photodissociation yield and resilience of the peptide bond were attributed to rapid internal conversion and efficient vibrational relaxation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Thaddeus W. Golbek, Halil I. Okur, Sergey Kulik, Jan Dedic, Sylvie Roke, Tobias Weidner
Summary: When a nanoparticle enters a biological environment, it quickly forms a dense protein layer called the protein corona. Using ultrafast sum frequency scattering spectroscopy, researchers can determine the structure and mode of action of biomolecules at interfaces. In this study, the action of lysozyme on lipid monolayers was investigated using sum frequency scattering spectroscopy.
Article
Chemistry, Multidisciplinary
Daria Maltseva, Sayantan Chatterjee, Chun-Chieh Yu, Mateusz Brzezinski, Yuki Nagata, Grazia Gonella, Anastasia C. Murthy, Jeanne C. Stachowiak, Nicolas L. Fawzi, Sapun H. Parekh, Mischa Bonn
Summary: Biomolecular condensates are dynamic membrane-less organelles that are rich in proteins and play important roles in various subcellular processes. However, abnormal phase transitions of disordered proteins in biomolecular condensates can lead to the formation of irreversible fibrils and aggregates, which are associated with neurodegenerative diseases. This study investigates the role of hydrophobic interactions in the phase transitions of the low-complexity domain of the FUS protein. The findings show that a hydrophobic interface drives fibril formation and molecular ordering of FUS at the air/water interface, resulting in solid-like film formation.
Article
Multidisciplinary Sciences
Lucas Gewehr, Benedikt Junglas, Ruven Jilly, Johannes Franz, Wenyu Eva Zhu, Tobias Weidner, Mischa Bonn, Carsten Sachse, Dirk Schneider
Summary: The dynamin-like protein SynDLP in the cyanobacterium Synechocystis sp. PCC 6803 forms ordered oligomers and interacts with negatively charged thylakoid membrane lipids independently of nucleotides. The structural characteristics of SynDLP suggest it to be a bacterial ancestor of eukaryotic dynamin.
NATURE COMMUNICATIONS
(2023)
Letter
Chemistry, Multidisciplinary
Arianna Marchioro, Thaddeus W. Golbek, Adam S. Chatterley, Tobias Weidner, Sylvie Roke
COMMUNICATIONS CHEMISTRY
(2023)
Article
Chemistry, Physical
Thaddeus W. Golbek, Tobias Weidner
Summary: The orientation of proteins on nanoparticle surfaces plays a crucial role in determining the toxicity, function, and identity of the nanoparticles. This study reveals that protein orientation on nanoparticle surfaces is influenced by coulombic forces between peptides across different particle volumes. The findings suggest that flat model systems are limited in accurately predicting protein orientation and that charge interactions should be considered in the design and assessment of medical nanoparticles.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Multidisciplinary Sciences
Steven J. Roeters, Kris Strunge, Kasper B. Pedersen, Thaddeus W. Golbek, Mikkel Bregnhoj, Yuge Zhang, Yin Wang, Mingdong Dong, Janni Nielsen, Daniel E. Otzen, Birgit Schiott, Tobias Weidner
Summary: This study reports a mechanism explaining why lipid membranes catalyze the formation of harmful aggregates at elevated concentrations.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Jan Thogersen, Tobias Weidner, Frank Jensen
Summary: The primary photolysis dynamics of aqueous carbonate and hydrogen carbonate when excited at lambda = 200 nm were studied using UV pump-Vis probe and UV pump-IR probe transient absorption spectroscopy and density functional theory calculations. The dissociation and electron detachment processes were observed, as well as the assistance of water molecules in the dissociation of CO32-. The primary photolysis of hydrogen carbonate was obscured by the photo-products of carbonate, but the two-photon ionization of water allowed for the study of aqueous carbonic acid.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Physical
Andrew P. Carpenter, Patricia Khuu, Tobias Weidner, Colin P. Johnson, Steven J. Roeters, Joe E. Baio
Summary: Dysferlin plays a critical role in muscle membrane repair by recruiting vesicles to the injured site. Research shows that its binding orientation is influenced by the lipid composition of the membrane.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Chemistry, Physical
Kris Strunge, Tucker Burgin, Thaddeus W. Golbek, Steven J. Roeters, Jim Pfaendtner, Tobias Weidner
Summary: α-synuclein folds into a defined secondary structure at the air-water interface, promoting its aggregation and fibrillation. Understanding the role of hydrophobic surfaces in this process is important for studying severe brain disorders such as Parkinson's disease.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Mohammadhasan Hedayati, Yuan- Chen, Justin R. Houser, Yujen Wang, Sajjad Norouzi, Hsin-Chih Yeh, Sapun H. Parekh
Summary: This study utilizes intramolecular Forster resonance energy transfer (FRET) and fluorescence lifetime microscopy (FLIM) to investigate molecular changes in fibrin protein with high spatial and temporal resolution. The results provide insights into the conformational changes of fibrin protein during macroscopic loading, allowing for the direct visualization of biomaterial mechanics and structure in cell-ECM scaffolds.
CHEMICAL COMMUNICATIONS
(2023)