Article
Multidisciplinary Sciences
Kaori Sano, Shinji Saito, Tadaki Suzuki, Osamu Kotani, Akira Ainai, Elly van Riet, Koshiro Tabata, Kumpei Saito, Yoshimasa Takahashi, Masaru Yokoyama, Hironori Sato, Takahiro Maruno, Kaede Usami, Susumu Uchiyama, Kiyoko Ogawa-Goto, Hideki Hasegawa
Summary: The functional properties of anti-HA stalk IgA antibodies are impacted by IgA polymerization, but unlike anti-HA head antibodies, this impact is not always positive. The coordination between two binding modules determines whether IgA polymerization enhances or diminishes the anti-viral functions of anti-HA stalk IgA antibodies.
Article
Virology
Wentao Shen, Qian Wang, Zhengxiang Wang, Minxuan Liu, Yingying Du, Lvfeng Yuan, Lu Han, Krzysztof Smietanka, Hualan Chen, Shuai Xu, Qiyun Zhu
Summary: The H7N9 subtype influenza A viruses pose a serious threat to public health, and there is still a lack of vaccines or drugs for humans against H7N9 influenza viruses. In this study, two monoclonal antibodies (MAbs), 4H1E8 and 7H9A6, were identified to specifically recognize and neutralize the H7N9 virus. These antibodies inhibit the conformational change of HA and block the HA-mediated membrane fusion, showing promising prophylactic and therapeutic effects against H7N9 virus infection. These findings suggest that these antibodies could be potentially used for the prevention and treatment of H7N9 influenza virus infection.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Louisa E. Wallace, Erik de Vries, Frank J. M. van Kuppeveld, Cornelis A. M. de Haan
Summary: This study systematically analyzed the role of sialoglycan-cleaving neuraminidase (NA) in viral entry in relation to sialoglycan-binding hemagglutinin (HA) receptor-binding preference, the receptor repertoire displayed on cells, and the presence of mucus decoy receptors. It was found that the dependency on NA activity for influenza A virus (IAV) entry largely depends on HA, with a2-6 sialoglycan-binding viruses being more inhibited by NA inhibitor than a2-3 sialoglycan-preferring viruses. The results indicate that the receptor-binding properties of HA in combination with the receptor repertoire present on cells determine the dependency of IAV on NA activity for entry.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alexander Pralow, Marcus Hoffmann, Terry Nguyen-Khuong, Markus Pioch, Rene Hennig, Yvonne Genzel, Erdmann Rapp, Udo Reichl
Summary: Glycosylation is an important quality attribute for biopharmaceutical production, and the glycan patterns of viral proteins can impact their virulence and immunogenicity. The study focused on analyzing N-glycan structures of HA and NA proteins in IAV, showing differences in glycan microheterogeneity and glycan structures, with variations in individual structure abundances.
Article
Biochemistry & Molecular Biology
Elisa Franzi, Gregory Mathez, Soraya Dinant, Charlotte Deloizy, Laurent Kaiser, Caroline Tapparel, Ronan Le Goffic, Valeria Cagno
Summary: Influenza virus is a major cause of respiratory infections worldwide, and finding alternative antiviral strategies is necessary despite the availability of vaccines and antiviral drugs. This study identifies non-steroidal estrogens as potent inhibitors of influenza virus by interacting with the hemagglutinin protein to prevent viral entry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Irina Alymova, John F. Cipollo, Lisa M. Parsons, Nedzad Music, Ram P. Kamal, Wen-Pin Tzeng, Cynthia S. Goldsmith, Joseph N. Contessa, Kevan L. Hartshorn, Jason R. Wilson, Hui Zeng, Shane Gansebom, Ian A. York
Summary: Individuals with metabolic dysregulation of cellular glycosylation often experience severe influenza disease, with reduced immune response and vaccine efficacy. Our study reveals that imbalanced glycosylation can modify the viral glycome without genomic changes, leading to reduced host immune responses and vaccine efficacy.
Article
Biochemistry & Molecular Biology
Won-Kyung Cho, Myong-Min Lee, Jin Yeul Ma
Summary: Isoquercitrin (IQC) has been shown to exhibit strong anti-influenza A virus infection by preventing viral attachment, entry, and release through virucidal effects. This makes IQC a potential candidate for the development of antiviral drugs against influenza viral infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Chenxin Han, Ziwei Xie, Yadi Lv, Dingxiang Liu, Ruiai Chen
Summary: This study reveals the important role of GRP78 in the adsorption stage of the NDV infection cycle and identifies the critical domain required for GRP78-HN interaction, providing new insights into the molecular mechanisms involved in NDV replication and infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Hemat S. Elsayed, Amany Adel, Dalal S. Alshaya, Fatmah A. Safhi, Areej S. Jalal, Dalia M. A. Elmasry, Karim Selim, Ahmed A. Erfan, Samah Eid, Samy Selim, Mohamed T. El-Saadony, Momtaz Shahein
Summary: In late 2016, different genotypes of highly pathogenic avian influenza virus were reported among domestic and wild bird species. The virus became endemic in poultry, causing significant economic losses. This study found the presence of the virus in ostriches and identified its genetic characteristics.
Article
Immunology
Luca T. Giurgea, Adriana Cervantes-Medina, Kathie-Anne Walters, Kelsey Scherler, Alison Han, Lindsay M. Czajkowski, Holly Ann Baus, Sally Hunsberger, Sabra L. Klein, John C. Kash, Jeffery K. Taubenberger, Matthew J. Memoli
Summary: In this study, it was found that females were more likely to have symptoms and a higher number of symptoms than males after influenza challenge. Neuraminidase inhibition (NAI) titers were found to be predictive of all outcomes of interest.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Pavithra Daulagala, Brian R. Mann, Kathy Leung, Eric H. Y. Lau, Louise Yung, Ruipeng Lei, Sarea I. N. Nizami, Joseph T. Wu, Susan S. Chiu, Rodney S. Daniels, Nicholas C. Wu, David Wentworth, Malik Peiris, Hui-Ling Yen
Summary: Immune imprinting shapes the anti-HA antibody landscape, but the evaluation of anti-HA and anti-NA antibody responses in individuals born within the same birth cohort has been limited. This study characterized NA antigenic variants and found age-dependent imprinting of both anti-HA and anti-NA antibodies. Including NA proteins in influenza vaccine preparations may enhance vaccine efficacy.
Article
Microbiology
Zhao Wang, Jieshi Yu, Zizhang Sheng, Ben M. Hause, Feng Li, Radhey S. Kaushik, Dan Wang
Summary: The N-terminal B-loop and CD helix of the HA2 protein in swine influenza A virus play a significant role in virus replication, with mutations in these regions attenuating virus replication. These mutations could potentially be explored as attenuated vaccine candidates to prevent the spread of influenza within swine populations.
VETERINARY MICROBIOLOGY
(2021)
Article
Cell Biology
Qi Wen Teo, Yiquan Wang, Huibin Lv, Timothy J. C. Tan, Ruipeng Lei, Kevin J. Mao, Nicholas C. Wu
Summary: This study reveals the importance of light-chain and CDR H3 sequences for the HA stem specificity of IGHV1-69 antibodies, providing molecular insights into broadly neutralizing antibody responses to influenza virus and important implications for universal influenza vaccine development.
Article
Chemistry, Multidisciplinary
Victor K. Outlaw, Ross W. Cheloha, Eric M. Jurgens, Francesca T. Bovier, Yun Zhu, Dale F. Kreitler, Olivia Harder, Stefan Niewiesk, Matteo Porotto, Samuel H. Gellman, Anne Moscona
Summary: Lower respiratory tract infections in children globally are largely caused by parainfluenza viruses (HPIVs), with no available vaccines or effective treatments against these pathogens. Research has shown that lipopeptides derived from HPIV3 F can inhibit infection, and the modification of the peptide backbone with beta-amino acid residues has resulted in improved antiviral activity and in vivo stability.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Qiuyu J. Huang, Kangkang Song, Chen Xu, Daniel N. A. Bolon, Jennifer P. Wang, Robert W. Finberg, Celia A. Schiffer, Mohan Somasundaran
Summary: Influenza viruses are highly pleomorphic, making their structural characterization challenging. This report demonstrates the use of convolutional neural networks and cryo-electron tomography to successfully characterize the morphology and glycoprotein density of the H1N1 influenza strain. This approach improves the efficiency of influenza virus analysis and has potential applications in studying other pleomorphic viruses and infected cells.
Article
Virology
Meiling Dai, Hongbo Guo, Jos C. F. M. Dortmans, Jojanneke Dekkers, Johan Nordholm, Robert Daniels, Frank J. M. van Kuppeveld, Erik de Vries, Cornelis A. M. de Haan
JOURNAL OF VIROLOGY
(2016)
Article
Cell Biology
Johan Nordholm, Jeanne Petitou, Henrik Ostbye, Diogo V. da Silva, Dan Dou, Hao Wang, Robert Daniels
JOURNAL OF CELL BIOLOGY
(2017)
Article
Cell Biology
Dan Dou, Ivan Hernandez-Neuta, Hao Wang, Henrik Ostbye, Xiaoyan Qian, Swantje Thiele, Patricia Resa-Infante, Nancy Mounogou Kouassi, Vicky Sender, Karina Hentrich, Peter Mellroth, Birgitta Henriques-Normark, Guelsah Gabriel, Mats Nilsson, Robert Daniels
Article
Multidisciplinary Sciences
Shady Younis, Wael Kamel, Tina Falkeborn, Hao Wang, Di Yu, Robert Daniels, Magnus Essand, Jorma Hinkula, Goran Akusjarvi, Leif Andersson
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Virology
Diogo V. da Silva, Johan Nordholm, Dan Dou, Hao Wang, Jeremy S. Rossman, Robert Daniels
JOURNAL OF VIROLOGY
(2015)
Article
Microbiology
Hao Wang, Dan Dou, Henrik Ostbye, Rebecca Revol, Robert Daniels
NATURE MICROBIOLOGY
(2019)
Article
Virology
Henrik Ostbye, Jin Gao, Mira Rakic Martinez, Hao Wang, Jan-Willem de Gier, Robert Daniels
JOURNAL OF VIROLOGY
(2020)
Correction
Microbiology
Hao Wang, Dan Dou, Henrik Ostbye, Rebecca Revol, Robert Daniels
NATURE MICROBIOLOGY
(2020)
Article
Microbiology
Jin Gao, Hongquan Wan, Xing Li, Mira Rakic Martinez, Laura Klenow, Yamei Gao, Zhiping Ye, Robert Daniels
Summary: Virions are commonly used as antigen source for viral vaccines, with the antigen abundance determined by the content of each protein in the virus. By exchanging viral backbone internal genes, the NA to HA ratio in virions can be improved to produce vaccines with higher NA content that can elicit more balanced neutralizing antibody responses to NA and HA. This approach could help improve the suboptimal efficacy of current influenza vaccines and provide broader coverage against circulating strains.
Article
Virology
Jin Gao, Laura Klenow, Lisa Parsons, Tahir Malik, Je-Nie Phue, Zhizeng Gao, Stephen G. Withers, John Cipollo, Robert Daniels, Hongquan Wan
Summary: This study compared several rNAs produced by the baculovirus/insect cell system with different tetramerization motifs and NA domains, and found that the combination of tetramerization and NA domains significantly impacts the biochemical, immunogenic, and protective properties of the rNAs. Single-dose immunizations were shown to be more informative for evaluating the immune response and protective efficacy of rNAs.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Jin Gao, Xing Li, Laura Klenow, Tahir Malik, Hongquan Wan, Zhiping Ye, Robert Daniels
Summary: This study compared the antigenicity of neuraminidase (NA) antigens from recent influenza vaccine strains and circulating viruses. The results showed that NAs from circulating H1N1 viruses and vaccine strains for the 2017-2021 seasons are antigenically similar but distinct from the previous H1N1 strain. Changes in N1 antigenicity were attributed to accumulated substitutions over time. NAs from circulating H3N2 viruses and the 2020-2021 vaccine strains showed similar antigenicity but varied across different seasons.
Article
Biochemical Research Methods
Tahir Malik, Laura Klenow, Alexandros Karyolaimos, Jan-Willem de Gier, Robert Daniels
Summary: Reverse genetics systems have been used to study viral replication and pathogenesis, as well as for therapeutic development. In this study, the stability of genes encoding influenza surface antigens was investigated when cloned into a common plasmid and transformed into E. coli. It was found that incorporating lac operators or a transcriptional terminator into the plasmid reduced gene expression and stabilized the viral genes. Sandwiching the viral gene between two lac operators provided the greatest stability. Stabilization was shown to be Lac repressor-dependent and crucial for plasmid propagation in E. coli.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Infectious Diseases
Anoopjit Singh Kooner, Yue Yuan, Hai Yu, Hyeog Kang, Laura Klenow, Robert Daniels, Xi Chen
Summary: Sialidases or neuraminidases are enzymes that cleave sialic acid and are expressed by various organisms, including pathogens. This study found that influenza enzymes have a more promiscuous substrate binding pocket.
INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Guillermo Moreno-Pescador, Mohammad Reza Arastoo, Victoria Thusgaard Ruhoff, Salvatore Chiantia, Robert Daniels, Poul Martin Bendix
Summary: Many cellular processes involve the lateral organization of membrane proteins into nanoscale domains. The mechanisms behind the clustering of membrane proteins into nanoscale lipid domains are not well understood. In this study, a method combining optical trapping, thermo-plasmonic-mediated membrane fusion, and confocal imaging was developed to investigate the phase affinity of membrane proteins. It was observed that the transferred membrane proteins partitioned into the liquid disordered phase of the model membranes. This generic platform allows the study of phase affinity for any plasma membrane protein labeled with a fluorescent marker.