期刊
FRONTIERS IN IMMUNOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2018.00118
关键词
antibody display technology; next-generation sequencing; phage display; antibody libraries; in vitro selection; antibody therapeutics
类别
资金
- Australian National Health and Medical Research Council [APP1090875, APP1148051, APP1113904, APP1113790]
- Australian Research Council [DP16010491]
In vitro selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage- and other antibody-display libraries, as well as NGS platforms and analysis tools. Moreover, we discuss recent examples relating to the use of NGS to assess library diversity, clonal enrichment, and affinity maturation.
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