Article
Biology
Shojiro Haji, Taiki Ito, Carla Guenther, Miyako Nakano, Takashi Shimizu, Daiki Mori, Yasunori Chiba, Masato Tanaka, Sushil K. Mishra, Janet A. Willment, Gordon D. Brown, Masamichi Nagae, Sho Yamasaki
Summary: This study reports that human Dectin-1 acts as a ligand for CLEC-2 and highlights the importance of glycosylation in its function as a CLEC-2 ligand. Furthermore, it is shown that expression of human Dectin-1 can rescue the lethality and lymphatic defect resulting from a deficiency of Podoplanin.
Article
Psychiatry
Diego Fabian Karvat Gracia, Eloisa Maria Pontarolo Gomes, Tamires Amelotti Coelho, Marcelo Carriello, Fabiana Antunes de Andrade, Lorena Bavia, Iara Jose Messias-Reason, Raffael Massuda
Summary: The study analyzed 49 patients with schizophrenia and found a negative association between the Positive and Negative Syndrome Scale total score and ficolin activation, and a positive association between body mass index and serum levels of C3 and CRP. MBL activation was not associated with any independent variables. More studies with larger sample populations are needed to confirm the results.
PSYCHIATRY RESEARCH
(2021)
Article
Immunology
Tianhang Li, Tianyao Liu, Zihan Zhao, Yuchen Pan, Xinyan Xu, Yulin Zhang, Shoubin Zhan, Shengkai Zhou, Wenjie Zhu, Hongqian Guo, Rong Yang
Summary: Immunotherapies offer new treatment options for urothelial bladder cancer, but their efficacy is limited by heterogeneity and therapeutic resistance. Research has shown that the commensal microbiota and its interactions with host immunity play a crucial role in bladder cancer. The urinary mycobiome and C-type lectin receptors (CLRs) may be potential targets for immunotherapy in bladder cancer.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Jan Dohnalek, Tereza Skalova
Summary: The C-type lectin-like fold (CTL fold) is a versatile structural motif found in many proteins, and its interactions play significant roles in various biological processes. Understanding the rules governing these interactions can aid in the development of new protein binders for biomedical and biotechnological applications.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Fisheries
Yang Liu, Zuzhen Wang, Wenjun Wang, Bing Liu, Chunfang Li, Yuandong Sun, Jiri Cao, Kuanyu Xia, Moci Yang, Jinpeng Yan
Summary: In this study, a novel C-type lectin (MaCTL) was cloned and characterized, which plays significant roles in innate immune response through binding to the pathogen-associated molecular patterns (PAMPs) on microorganisms. The results showed that MaCTL is widely expressed in various tissues and upregulated after LPS stimulation and Aeromonas hydrophila challenge, suggesting its involvement in innate immune response. Furthermore, MaCTL can bind to various bacteria and carbohydrates, and exhibit antimicrobial effects.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Cell Biology
Mengfan Zhang, Yang Jing, Wenze Xu, Xiaojing Shi, Wenguang Zhang, Pengfei Chen, Xiaocang Cao, Xinwei Han, Xuhua Duan, Jianzhuang Ren
Summary: It has been found that the C-type lectin COLEC10 is predominantly produced by hepatic stellate cells and is involved in the pathogenesis of liver fibrosis.
CELL DEATH & DISEASE
(2023)
Article
Fisheries
Xiaohan Liu, Jiqing Gao, Xiao Wei, Xinze Zhang, Liping You, Yuanjin Liu, Lizhu Chen, Caili Liu, Chunxiao Sun, Xiuhui Tian, Quanli Zhou, Xiaomin Zhang
Summary: In this study, the potential mechanisms of PAMP binding and opsonic activities of a c-type lectin and a sialic acid-binding lectin from manila clam were investigated. The results showed that both lectins could bind to various pathogen-associated molecular patterns (PAMPs) and enhance the phagocytosis and encapsulation ability of hemocytes. These lectins may function as pattern recognition receptors (PRRs) involved in the innate immune responses of the clam.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Microbiology
Axel Dietschmann, Sebastian Schruefer, Stefanie Westermann, Fiona Henkel, Kirstin Castiglione, Ralf Willebrand, Jasmin Adam, Jurgen Ruland, Roland Lang, Donald C. Sheppard, Julia Esser-von-Bieren, Daniel Radtke, Sven Krappmann, David Voehringer
Summary: Eosinophilia is associated with various inflammatory diseases, such as allergic bronchopulmonary aspergillosis (ABPA). It has been shown that direct interaction between eosinophils and Aspergillus fumigatus is essential for the activation of eosinophils, which then secrete multiple proinflammatory mediators. The PI3K-AKT-mTOR pathway plays a crucial role in A. fumigatus-induced eosinophil activation.
Article
Immunology
Camille Niveau, Eleonora Sosa Cuevas, Benoit Roubinet, Mylene Pezet, Michel Thepaut, Stephane Mouret, Julie Charles, Franck Fieschi, Ludovic Landemarre, Laurence Chaperot, Philippe Saas, Caroline Aspord
Summary: Dendritic cell subsets play a crucial role in shaping anti-tumour immunity. The study found that melanoma tumour cells display aberrant glycan motifs which can be sensed by dendritic cells through specific receptors. This interaction may lead to abnormal immune responses. The study suggests that targeting the glycan/receptor axis could be a promising strategy for improving cancer immunotherapy.
Article
Fisheries
Crystal Guluarte, Martha Reyes-Becerril, Sergio Rosales-Mendoza, Carlos Angulo
Summary: This study analyzed the MINCLE receptor in Pacific red snapper and investigated the stimulating effects of beta-glucans and antigenic proteins on immune cells. The results showed that beta-glucans enhanced respiratory burst activity and nitric oxide production, and up-regulated receptor and pro-inflammatory cytokine gene expression. These findings highlight the significance of beta-glucans and their receptors in the innate immune response.
AQUACULTURE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ankur Midha, Guillaume Goyette-Desjardins, Felix Goerdeler, Oren Moscovitz, Peter H. Seeberger, Karsten Tedin, Luca D. Bertzbach, Bernd Lepenies, Susanne Hartmann
Summary: Ascariasis is a global health issue affecting both humans and animals, with adult Ascaris nematodes forming chronic infections in the host intestine by interacting with host cells and the microbiota. The parasite-secreted protein AsCTL-42 plays a role in the triad relationship between Ascaris, host cells, and the microbiota, by neutralizing bacterial cells without killing them and reducing invasion of intestinal epithelial cells by Salmonella.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Fisheries
Bao-Rui Zhao, Xin-Xin Wang, Peng-ping Liu, Xian-Wei Wang
Summary: This review summarizes the current knowledge and functions of complement-related proteins in crustaceans, and compares the pro-phenoloxidase activating system in crustaceans with the complement system in mammals.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Immunology
Huanghui Wu, Han Bao, Cong Liu, Qiao Zhang, Ailing Huang, Minxue Quan, Chunhui Li, Ying Xiong, Guozhong Chen, Lichao Hou
Summary: This study investigated the role and mechanism of extracellular nucleosomes and histones, as damage-associated molecular patterns (DAMPs), in progressive neuroinflammation and chronic stress-related emotional disorders. It found that extracellular nucleosomes accelerate microglial inflammation via Clec2d and TLR9, contributing to chronic stress-induced emotional disorders.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Francois Bulteau, Michel Thepaut, Maxime Henry, Amandine Hurbin, Laetitia Vanwonterghem, Corinne Vives, Aline Le Roy, Christine Ebel, Olivier Renaudet, Franck Fieschi, Jean-Luc Coll
Summary: This study found that recombinant MGL fragments can efficiently target Tn-positive human tumors without inducing toxicity, making it a promising candidate for in vivo diagnosis and imaging of human tumors and potentially for therapeutic applications.
MOLECULAR PHARMACEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Lydia Gonzalez-del-Barrio, Laura Perez-Alos, Leon Cyranka, Anne Rosbjerg, Simon Nagy, Zoltan Prohaszka, Peter Garred, Rafael Bayarri-Olmos
Summary: The complement system is a complex cascade involved in pathogen defense and disease pathogenesis. Complement modulators, such as the fused MAP-2:CD55(1-4) inhibitor, show promising potential for treating complement-driven inflammatory diseases. This chimeric inhibitor effectively inhibits complement activation at multiple levels and demonstrates strong inhibitory activity in vitro.
Letter
Critical Care Medicine
Olivia C. Leavy, Shwu-Fan Ma, Philip L. Molyneaux, Toby M. Maher, Justin M. Oldham, Carlos Flores, Imre Noth, R. Gisli Jenkins, Frank Dudbridge, Louise V. Wain, Richard J. Allen
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2021)
Article
Ecology
Mohammad H. Mirhakkak, Sascha Schaeuble, Tilman E. Klassert, Sascha Brunke, Philipp Brandt, Daniel Loos, Ruben Uribe, Felipe Senne de Oliveira Lino, Yueqiong Ni, Slavena Vylkova, Hortense Slevogt, Bernhard Hube, Glen J. Weiss, Morten O. A. Sommer, Gianni Panagiotou
Summary: The study reconstructed a metabolic model of Candida albicans to investigate bacterial-fungal metabolic interactions in the gut, predicting key gut bacterial species modulating C. albicans colonization levels. The findings were confirmed through metagenomic sequencing and fungal growth experiments, demonstrating the potential impact of gut microbiome on harmful levels of C. albicans.
Article
Infectious Diseases
Long T. Hoang, Pooja Jain, Timesh D. Pillay, Mica Tolosa-Wright, Umar Niazi, Yemisi Takwoingi, Alice Halliday, Luis C. Berrocal-Almanza, Jonathan J. Deeks, Peter Beverley, Onn Min Kon, Ajit Lalvani
Summary: This study evaluated the diagnostic accuracy of blood transcriptomic signatures in patients with suspected tuberculosis, but found that they lacked sufficient accuracy for diagnosis in routine clinical practice. Transcriptional signatures were not clinically useful for diagnosing suspected tuberculosis, even in patients with highly probable tuberculosis where there is a great unmet need.
LANCET INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Julia Alcoba-Florez, Helena Gil-Campesino, Diego Garcia-Martinez de Artola, Oscar Diez-Gil, Agustin Valenzuela-Fernandez, Rafaela Gonzalez-Montelongo, Laura Ciuffreda, Carlos Flores
Summary: This study aimed to increase testing capacity for COVID-19 in Spain through sample pooling and found that a pool size of five samples had higher sensitivity compared to larger pool sizes. Utilizing sample pooling strategy with a pool size of five allowed for efficient detection of individual positive samples in positive pools with minimal loss of sensitivity.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Editorial Material
Dermatology
C. Flores
BRITISH JOURNAL OF DERMATOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Eva Suarez-Pajes, Ana Diaz-de Usera, Itahisa Marcelino-Rodriguez, Beatriz Guillen-Guio, Carlos Flores
Summary: Admixed populations result from interbreeding of two or more ancestral populations, with genomes defined by inherited genetic tracts from these parental groups. Various methods can be used to determine the ancestry apportionment of admixed individuals, aiding in the discovery of genetic loci linked to diseases and traits. Admixture mapping studies in human diseases have significant biomedical implications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Alice Halliday, Tereza Masonou, Mica R. Tolosa-Wright, Yanping Guo, Long Hoang, Robert Parker, Aime Boakye, Yemisi Takwoingi, Amarjit Badhan, Pooja Jain, Ishita Marwah, Luis C. Berrocal-Almanza, Jonathan Deeks, Peter Beverley, Onn Min Kon, Ajit Lalvani
Summary: Measurement of Mtb-specific cellular immune signatures by flow cytometry can differentiate active TB from LTBI, improving and accelerating the diagnosis of tuberculosis.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Geriatrics & Gerontology
Katharina Busch, Melanie Kny, Nora Huang, Tilman E. Klassert, Magdalena Stock, Alexander Hahn, Sebastian Graeger, Mihail Todiras, Sibylle Schmidt, Bishwas Chamling, Michael Willenbrock, Stefan Gross, Doreen Biedenweg, Arnd Heuser, Claus Scheidereit, Christian Butter, Stephan B. Felix, Oliver Otto, Friedrich C. Luft, Hortense Slevogt, Jens Fielitz
Summary: The study showed that in septic cardiomyopathy, Nlrp3 knockout mice prevented cardiac atrophy and improved diastolic cardiac function. IL-1 beta caused cardiomyocyte atrophy, impaired contractility and relaxation, and decreased cell deformation.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2021)
Article
Microbiology
Tilman E. Klassert, Rasmus Leistner, Cristina Zubiria-Barrera, Magdalena Stock, Mercedes Lopez, Robert Neubert, Dominik Driesch, Petra Gastmeier, Hortense Slevogt
Summary: The study revealed that the bacterial community structure in hospitals can quickly stabilize within a few weeks after patient occupancy, with a significant increase in bacterial biomass and diversity during the colonization process. Additionally, the results highlighted a rise in antibiotic resistance determinants on hospital floors, emphasizing the importance of infection control strategies in healthcare settings.
Article
Cell Biology
Esther Klaile, Juan P. Prada Salcedo, Tilman E. Klassert, Matthias Besemer, Anne-Katrin Bothe, Adrian Durotin, Mario M. Mueller, Verena Schmitt, Christian H. Luther, Marcus Dittrich, Bernhard B. Singer, Thomas Dandekar, Hortense Slevogt
Summary: The study demonstrates that ligation of CEACAM receptors has differential effects on the immune functions of human neutrophils in response to C. albicans, altering apoptosis, cytokine secretion, and other responses.
CELLULAR IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katrin Boettcher, Kerstin Braunschmidt, Gianna Hirth, Karsten Schaerich, Tilman E. Klassert, Magdalena Stock, Janine Sorgatz, Sabine Fischer-Burkart, Steffen Ullrich, Samantha Frankenberger, Daniel Kritsch, Christian Kosan, Ralf Kueppers, Lothar J. Strobl, Hortense Slevogt, Ursula Zimber-Strobl, Berit Jungnickel
Summary: p53 plays a crucial role in maintaining genome stability by regulating DNA repair and translesion synthesis. Mice lacking p53 show altered translesion polymerase-mediated mutagenesis, suggesting a potential role of p53 in non-B cells affecting B cell mutations. Inhibition of p53 leads to increased somatic hypermutation in human B lymphoma cells, indicating that loss of p53 function may promote genetic instability in vivo during antibody diversification.
MOLECULAR IMMUNOLOGY
(2021)
Article
Infectious Diseases
Tilman E. Klassert, Cristina Zubiria-Barrera, Robert Neubert, Magdalena Stock, Antony Schneegans, Mercedes Lopez, Dominik Driesch, Gregor Zakonsky, Petra Gastmeier, Hortense Slevogt, Rasmus Leistner
Summary: This study investigates the effects of different sanitization strategies on the microbial structures and the ecological balance of the environmental microbiome in the clinical setting. The results show that probiotic sanitization leads to changes in the environmental microbiota and a significant reduction in antibiotic resistance genes.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Public, Environmental & Occupational Health
Luis C. Berrocal-Almanza, Ross J. Harris, Simon M. Collin, Morris C. Muzyamba, Olivia D. Conroy, Adil Mirza, Anne-Marie O'connell, Lynn Altass, Sarah R. Anderson, H. Lucy Thomas, Colin Campbell, Dominik Zenner, Nick Phin, Onn Min Kon, E. Grace Smith, Ajit Lalvani
Summary: This study evaluated the effectiveness of a primary care-based ITBI testing and treatment programme for migrants from high-incidence countries in high tuberculosis incidence areas in England. The results showed that the programme helped to diagnose active tuberculosis earlier and lower the risk of progression to tuberculosis.
LANCET PUBLIC HEALTH
(2022)
Article
Immunology
Mario M. Mueller, Christian Baldauf, Stella Hornischer, Tilman E. Klassert, Antony Schneegans, Andrea Behnert, Mathias W. Pletz, Stefan Hagel, Hortense Slevogt
Summary: Exposure of human monocytes to Staphylococcus aureus or TLR2 ligands induces a similar tolerant cell state to that induced by LPS. In this state, pro-inflammatory gene expression is downregulated and glycoprotein expression changes are similar to other TLR ligands but also exhibit some distinct patterns in S. aureus-stimulated monocytes. Monocytes from patients with S. aureus bacteremia still retain pro-inflammatory responsiveness to S. aureus stimulation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Michelle Maurer, Tilman E. E. Klassert, Bettina Loeffler, Hortense Slevogt, Lorena Tuchscherr
Summary: This study presents a novel and straightforward strategy to isolate RNA from intracellular S. aureus after 90 min, 24 h, and 48 h postinfection. Real-time PCR data showed that the reference genes gyrB, aroE, tmRNA, gmk, and hu were the most stable under different conditions and were used for normalization. The extracted RNA from intracellular S. aureus showed low variability in acetylation levels during the early phase of infection, demonstrating its high quality.