期刊
APPLIED SCIENCES-BASEL
卷 8, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/app8060930
关键词
podocytes; half-life; chitosan; protein expression; scaffold
类别
资金
- CONACYT [377122]
- laboratory of virology and immunology of biological science faculty of the Universidad Autonoma de Nuevo Leon
Loss of podocytes has been a main pathology present in renal diseases; the leak of these specialized cells increases the permeability of the glomerular basal membrane (GMB) and protein release affecting the glomeruli, the main structure of the kidney. The study of different physiopathology mechanism has been a challenge because of the short lifetime of podocytes in vitro. We obtained and characterized composites based on Chitosan (CTS), polyvinyl alcohol (PVA), type IV collagen and integrin (31) as a possible application in primary culture of podocytes. Podocytes were extracted from the urine of patients with Idiopathic Nephrotic Syndrome (INS). To evaluate biocompatibility, we assessed cell viability through the lactate dehydrogenase assay. Immunohistochemical staining was used to detect the expression of specific proteins from podocytes such as podocin, and podocalyxin and CD80, a marker of cellular stress. The results showed that our synthesis method promotes the copolymerization of the components in the scaffold. Due to its reactivity, the amine group of chitosan made links with type IV collagen and integrin (31). The swelling and degradation tests allowed us to select the material with the best mechanical properties for cellular culture. The expression of podocin and podocalyxin remains the same in the culture of podocytes on the scaffold; in contrast, CD80 expression increased. The viability of podocytes cultured on the CTS/PVA/type IV collagen/integrin (31) scaffold increased in comparison to the culture control.
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